羟基磷灰石硅胶复合材料的细胞毒性检测

目的研究和开发羟基磷灰石硅胶复合材料,并对生产的4种组织代用品的细胞毒性进行了研究。方法将4种组织代用品的浸提液与宫颈癌HeLa细胞及淋巴母细胞AHH-1细胞接触2、3、5、6天后,用酶联免疫仪分别在492nm和570nm波长下测定各孔光吸收值,计算细胞相对增值率,用六级毒性分类法评级,并进行形态学观察。结果培养期4种材料浸提液培养的细胞均大量增殖,形态正常,毒性级为0～1级。结论羟基磷灰石硅胶复合材料具有良好的细胞生物相容性。     

羟基磷灰石纳米粒子抑制皮肤鳞状细胞癌细胞增殖的机制

目的：探讨羟基磷灰石(HAP)纳米溶胶抑制皮肤鳞癌细胞株(A431)增殖的作用机制。方法：实验组用含浓度为400mg/L HAP纳米溶胶的DMEM培养液培养A431细胞。在复合培养的第1、2、3、4、5天,通过免疫荧光显微镜观察A431细胞凋亡过程中形态结构的变化;透射电镜观察A431细胞超微结构的改变。结果：免疫荧光显微镜下显示,实验组在复合培养的第1、2天出现少量绿荧光阳性细胞,细胞形态不规则,第3天可见红绿荧光双标阳性细胞,第4、5天出现较多的红荧光阳性细胞,细胞的染色质聚集于核膜周围成块状或新月形;透射电镜下可见实验组在复合培养的第2天细胞内出现HAP纳米粒子,细胞形态各异,胞质空泡化,核仁裂解,染色质边集,部分细胞胞质脱落。结论：HAP纳米溶胶抑制A431细胞增殖的作用机制可能为：HAP纳米粒子进入细胞内,直接作用于细胞内的细胞器,引起细胞凋亡、胀亡和胞体割裂。     

羧甲基壳聚糖膜对人皮肤黑素细胞的生物相容性研究北大核心CSCD

目的 探讨羧甲基壳聚糖膜对健康人黑素细胞的生物相容性,探索羧甲基壳聚糖膜搭载黑素细胞的可行性.方法 使用流延法联合戊二醛交联法制备羧甲基壳聚糖膜,黑素细胞分离培养技术分离培养健康人黑素细胞,使用HMB45染色、MTT法、NaOH裂解法、酪氨酸酶活性检测等方法检测羧甲基壳聚糖膜对黑素细胞功能的影响.结果 倒置显微镜下观察,黑素细胞在羧甲基壳聚糖膜上分布均匀,形态正常.免疫组化染色显示,贴附于羧甲基壳聚糖膜的黑素细胞抗HMB45单克隆抗体染色阳性.利用MTT法测定的黑素细胞增殖曲线显示,羧甲基壳聚糖可以支持黑素细胞的正常生长,黑素合成量（A值为0.083±0.015）与培养皿所培养的黑素细胞（A值0.066±0.008）比较,差异无统计学意义（t=2.38,P＞0.01）;黑素细胞酪氨酸酶活性（4值0.234±0.083）与培养皿培养的黑素细胞（A值0.241±0.061）比较,差异无统计学意义（t=0.23,P＞0.05）.结论 羧甲基壳聚糖膜可以维持黑素细胞的正常功能,具有较好的生物相容性.     

新型生物创伤敷料的制备与研究

目的 以猪小肠黏膜下层为原料制备新型生物敷料.方法 按Abraham方法制备猪小肠黏膜下层,冻干后冷冻粉碎为直径15～30 μm的小肠黏膜下层粉末,将该粉末置于含有1%胃蛋白酶和3%乙酸的水溶液中混合消化48h,将得到的小肠黏膜下层溶液冷冻干燥制备成小肠黏膜下层海绵,最后采用碳化亚胺对干燥后的海绵进行交联改性,从而得到猪小肠黏膜下层海绵.通过形态结构、吸水率、降解性能等方面的研究,对其作为创伤敷料的有效性进行初步评价.结果 小肠黏膜下层海绵呈三维网孔状结构,孔径为100～200μm.1%SIS海绵具有很强的吸水能力并在磷酸缓冲液中具有较好的稳定性,1%SIS海绵在大鼠骨骼肌内埋置4w后可完全降解,周围骨骼肌纤维修复良好,无变性、坏死等不良现象发生.结论 猪SIS海绵具备作为新型生物敷料的潜能,具有止血、防渗出能力强的特点,能为细胞的生长和繁殖提供营养和代谢环境,可完全降解并达到组织永久性替代的目的.     

羟基磷灰石纳米粒子对A431细胞增殖抑制作用的研究

目前对皮肤鳞状细胞癌的治疗一般首选外科手术切除,但对一些不适宜皮肤外科治疗的癌前病变以及某些特殊部位如口腔内、阴茎等处皮损面积较大的病变,现有的治疗手段疗效均不理想.已有学者研究发现,羟基磷灰石(hydroxya-patite,HAP)纳米粒子具有抗癌活性[1-2].本课题研究HAP纳米溶胶对皮肤鳞癌细胞株(A431)细胞增殖的影响,为应用HAP纳米粒子治疗皮肤鳞状细胞癌提供实验依据.     

羟基磷灰石纳米粒子对A431细胞增殖抑制作用的研究

目前对皮肤鳞状细胞癌的治疗一般首选外科手术切除,但对一些不适宜皮肤外科治疗的癌前病变以及某些特殊部位如口腔内、阴茎等处皮损面积较大的病变,现有的治疗手段疗效均不理想.已有学者研究发现,羟基磷灰石(hydroxya-patite,HAP)纳米粒子具有抗癌活性[1-2].本课题研究HAP纳米溶胶对皮肤鳞癌细胞株(A431)细胞增殖的影响,为应用HAP纳米粒子治疗皮肤鳞状细胞癌提供实验依据.     

羟基磷灰石纳米粒子对A431细胞增殖抑制作用的研究

目前对皮肤鳞状细胞癌的治疗一般首选外科手术切除,但对一些不适宜皮肤外科治疗的癌前病变以及某些特殊部位如口腔内、阴茎等处皮损面积较大的病变,现有的治疗手段疗效均不理想.已有学者研究发现,羟基磷灰石(hydroxya-patite,HAP)纳米粒子具有抗癌活性[1-2].本课题研究HAP纳米溶胶对皮肤鳞癌细胞株(A431)细胞增殖的影响,为应用HAP纳米粒子治疗皮肤鳞状细胞癌提供实验依据.     

羟基磷灰石纳米粒子对A431细胞增殖抑制作用的研究

目前对皮肤鳞状细胞癌的治疗一般首选外科手术切除,但对一些不适宜皮肤外科治疗的癌前病变以及某些特殊部位如口腔内、阴茎等处皮损面积较大的病变,现有的治疗手段疗效均不理想.已有学者研究发现,羟基磷灰石(hydroxya-patite,HAP)纳米粒子具有抗癌活性[1-2].本课题研究HAP纳米溶胶对皮肤鳞癌细胞株(A431)细胞增殖的影响,为应用HAP纳米粒子治疗皮肤鳞状细胞癌提供实验依据.     

羟基磷灰石纳米粒子对A431细胞增殖抑制作用的研究

目前对皮肤鳞状细胞癌的治疗一般首选外科手术切除,但对一些不适宜皮肤外科治疗的癌前病变以及某些特殊部位如口腔内、阴茎等处皮损面积较大的病变,现有的治疗手段疗效均不理想.已有学者研究发现,羟基磷灰石(hydroxya-patite,HAP)纳米粒子具有抗癌活性[1-2].本课题研究HAP纳米溶胶对皮肤鳞癌细胞株(A431)细胞增殖的影响,为应用HAP纳米粒子治疗皮肤鳞状细胞癌提供实验依据.     

羟基磷灰石纳米粒子对A431细胞增殖抑制作用的研究

目前对皮肤鳞状细胞癌的治疗一般首选外科手术切除,但对一些不适宜皮肤外科治疗的癌前病变以及某些特殊部位如口腔内、阴茎等处皮损面积较大的病变,现有的治疗手段疗效均不理想.已有学者研究发现,羟基磷灰石(hydroxya-patite,HAP)纳米粒子具有抗癌活性[1-2].本课题研究HAP纳米溶胶对皮肤鳞癌细胞株(A431)细胞增殖的影响,为应用HAP纳米粒子治疗皮肤鳞状细胞癌提供实验依据.     

In vivo efficacy of biocompatible silver nanoparticles cream for empirical wound healing

Wound healing is a complicated process that begins at the onset of injury and a continued process till complete healing. The emergence of nanotechnology has provided a new therapeutic modality to silver nanoparticles in treatment of wounds. However, the safety of these silver nanoparticles in the process of wound healing is yet to be elucidated; nevertheless, biocompatibility is the primary concern. Biosynthesis of silver nanoparticles was synthesized using aqueous clove extract and silver nitrate solution under microwave and the obtained particles size were 30–60 nm and roughly spherical in shape. The present study focused on the efficacy of biocompatible silver nanoparticles in vivo wound healing process. Consequently, this study supported the incorporation of biosynthesized silver nanoparticles in wound dressings as a cream formulation for improved healthcare.     

Biocompatibility of microparticles into soft tissue fillers

The increasing need for long-lasting injectable soft tissue fillers for the treatment of wrinkles and folds requires a critical discussion of the biocompatibility on a scientific background. Since biological fillers made of collagen and hyaluronic acid will be resorbed over time, copolymer biomaterials with microparticles have been developed in recent years. The microparticles followed special and essential demands because of the interaction with the tissue. In search of an ideal soft tissue filler substance, a variety of biomaterials with microparticles suspended have been created for injecting into dermal defects, into the urethra of patients with urinary incontinence, and in patients with vocal cord insufficiency. The particles differ in chemical composition, surface structure, surface charge, and particle size and evoke different host reactions, accordingly.     

Intense pulsed light vs silicone gel sheet in the management of hypertrophic scars: an interventional comparative trial in the Indian population

Introduction: Hypertrophic scars are abnormal scars that are a source of great functional, psychological and financial burden to the patient. Currently available treatment modalities are either very cumbersome to use or have not provided very satisfactory results. Intense pulsed light (IPL) therapy for the management of hypertrophic scars has been recently introduced. However, most of the studies available are from the Western population. In this trial, we aim to study the effect of IPL on the South Indian population with darker skin and compare it with an existing treatment modality (silicone gel sheet). Materials and Methods: This trial was an interventional clinical trial conducted from September 2015 to March 2017 on 28 patients with 65 hypertrophic scars. Each scar was divided into 2 equal parts (4.6 x 1 cm2). One half of the scar was treated to 4 sessions of 29J/cm2 of IPL at 3 weekly intervals. A silicone gel sheet (SGS) was applied over the other half and changed at 3 weekly intervals. The Vancouver scar scale was used to grade both halves of the scar before treatment, at each visit and 3 weeks after treatment completion. Results: Scars treated with IPL showed a significant reduction in the pliability (p=0.002) and hyperpigmentation (p=0.000) as compared to scars treated with SGS. Though the percentage reduction in the height of the scar was more in the IPL group (15.4% as compared to 4.6%in the SGS group), the result was not statistically significant (p<0.065). As most scars in our study showed normal vascularity at the beginning, the percentage reduction in vascularity between both groups was the same (p<0.597). The reduction in the median VSS score was more in the IPL group compared to SGS group (p=0.00). Conclusion: IPL therapy offers a safe and effective means of hypertrophic scar treatment, especially on large scars where other treatment modalities may not be feasible. Minimal side effects that occur can be prevented with proper pre, intra and post procedure cooling.     

复方壳多糖真皮替代物体外抗菌作用的研究

目的 研究复方壳多糖真皮替代物对金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、白念珠菌的体外抑菌作用。方法 制备复方壳多糖真皮替代物和复方胶原凝胶真皮,分别为实验组和对照组,各取50μL滴加在涂满细菌或真菌的平板,每种菌实验组和对照组样本各6个,孵育24h后观察抑菌环。结果 复方壳多糖真皮替代物对金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌具有抑菌作用,抑菌环均大于复方胶原凝胶真皮替代物,差异有统计学意义。两组对白念珠菌无抑菌作用。结论 复方壳多糖真皮替代物是有体外抗细菌作用的组织工程产品。     

中药肌能饮联合壳聚糖季铵盐硅凝胶对瘢痕疙瘩修复的观察

目的探讨中药肌能饮联合壳聚糖季铵盐硅凝胶对瘢痕疙瘩修复的效果。方法 98例瘢痕疙瘩患者使用该方法治疗后3个月,按照显效、有效和无效标准进行疗效分析。结果用中药肌能饮联合壳聚糖季铵盐硅凝胶治疗瘢痕疙瘩患者3个月后,瘢痕疙瘩修复的有效率为93.9%,未见不良反应。3个月后随访,患者情况基本稳定。结论中药肌能饮联合壳聚糖季铵盐硅凝胶对瘢痕疙瘩的修复效果好,安全性高。     

Age-related changes in skin topography and microcirculation

Skin topography and microvasculature undergo characteristic changes with age. Although several non-invasive bioengineering methods are currently available to measure them quantitatively, few publications have referred to their relationship with age in different anatomical sites. This study was carried out to observe the age-related changes of the skin topography and skin microcirculation. The microrelief was assessed with special processing software from scanning by interference fringe profilometry of silicone replicas performed on two sites (volar forearm and back of hand) on 50 female volunteers (aged 20–74 years who consisted of ten probands in each decade). The superficial vascular network of both sites was assessed by videocapillaroscopy, and the subpapillary vascular plexus was studied with laser Doppler flowmetry. Skin color, which is affected by blood flow, was observed by colorimeter. The skin roughness and the mean height between peak and valley increased with age. There were statistically significant differences between the evaluated sites. This study also shows that the capillary loops in the dermal papillae decrease but the subpapillary plexus increase with age. The interference fringe profilometry associated with videocapillaroscopy may be useful and accurate to measure the efficacy of medical or cosmetic products to delay skin aging.     

Vitamin E-derived copolymers continue the challenge to hemodialysis biomaterials

Improving material biocompatibility has been a continuous effort and remains a major goal of dialysis therapy. In this respect, vitamin E-modified copolymers have been used to produce a generation of biomaterials that has offered new clinical challenges and the chance of further improving the quality of synthetic hemodialyser membranes. This mini review article describes the evolution of these copolymers that only recently have been adopted to develop new vitamin E-modified polysulfone hemodialysers. Biomaterial characteristics and clinical aspects of these membranes are discussed, starting from the most recent contributions that have appeared in the literature that are of interest for the community of nephrology and dialysis specialists, as well as biomaterial scientists.