厘米波致猪视网膜神经细胞损伤及硒的防护效应

目的：观察厘米波对体外培养的猪视网膜神经细胞的脂质过氧化损伤作用及亚硒酸钠的防护作用。方法：体外２猪视网膜神经细胞,按微波强度分为３组进行微波辐照１ｈ,选６０ｍＷ／ｃｍ＾２组进行防护在２液中加入不同浓度的亚硒酸钠后辐照,照后即刻测培养液温度、细胞风ＧＳＨ－Ｐｘ,ＳＯＤ活性、ＭＤＡ含量和细胞内Ｓｅ浓度,结果：辐照后ＧＳＨ－Ｐｘ和ＳＯＤ活性下降,ＭＤＡ含量升高,变化程度随微波辐照强度增大而增大,加亚硒     

微波诱导视网膜神经细胞凋亡及硒的保护作用

目的 观察微波诱导视网膜神经细胞凋亡及硒的保护作用。方法 体外培养视网膜神经细胞,按微波辐照强度分为４组辐照１ｈ,选３０ｍＷ．ｃｍ＾－２组为防护组,在培养液中加入Ｎａ２ＳｅＯ３后辐照,照后测培养液温度,细胞存活率,激光共聚焦显微镜观察细胞凋亡并进行荧光强度的定量分析。结果 培养液温度,细胞凋亡数量及荧光强度 随微波辐照强度增大而增加,加Ｎａ２ＳｅＯ３后辐照可使凋亡细胞数量减少,荧光强度减弱。结论微     

微波辐射对大鼠血中谷胱甘肽过氧化物酶的影响

本文报道了微波辐射对大鼠血中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性的影响。结果表明，除照射剂量４０ｍＷ／ｃｍ＾２组与对照组比较无显著性差异外（Ｐ〉０．０５），６０ｍＷ／ｃｍ＾２、８０ｍＷ／ｃｍ＾２与对照组比较存在高度显著性差异（Ｐ〈０．０１）。提示微波辐射在剂量大于６０ｍＷ／ｃｍ＾２时对大鼠体内谷胱甘肽过氧化物酶有损害作用。     

锌对微波致猪视网膜神经节细胞损伤的防护作用

目的 探讨微波对体外培养的猪视网膜神经节细胞的脂质过氧化损伤作用及耐受剂量,为进一步研究微波的眼度损伤机制及其防护提供一定的实验依据,方法 体外培养猪视网膜神经节细胞,按微波辐照强度分为对照组,30mW.cm^-2组,60mW.cm^-2组及各辐照剂量加锌组,微波理疗机于微波屏蔽室内辐照1h,辐照后立即于光镜及电镜观察细胞形态变化,测定超氧化物歧化酶（SOD）和丙二醛（MDA）活性。结果 微波辐照后,细胞有聚集现象,部分细胞轴突消失,电镜可见线粒体及内质网肿胀。细胞MDA活性明显增高,SOD降低,加锌各组光镜下细胞形态变化不明显,电镜显球线体轻度肿胀,嵴完整,MDA活性有所恢复,SOD活性增高。结论 微波可引起视网膜视经节细胞脂质过氧化损伤,锌可提高细胞的抗氧化能力,在一定程度上减轻微波对视网膜神经节细胞的过氧化损伤。     

微波辐射对大鼠血中谷胱甘肽过氧化物酶的影响

本文报道了微波辐射对大鼠血中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性的影响。结果表明，除照射剂量４０ｍＷ／ｃｍ２组与对照组比较无显著性差异外（Ｐ＞０．０５），６０ｍＷ／ｃｍ２、８０ｍＷ／ｃｍ２组与对照组比较存在高度显著性差异（Ｐ＜０．０１）。提示微波辐射在剂量大于６０ｍＷ／ｃｍ２时对大鼠体内谷胱甘肽过氧化物酶有损害作用。     

不同频率及功率密度毫米波辐射对子代学习记忆功能 …

为阐明毫米波对胚胎产生损伤效应的阈值及导致成年子鼠学习记忆功能降低的机理，验证毫米波有无频率特异性。用３７．４￣６０ＧＨｚ，功率密度１ｍＷ／ｃｍ＾２￣８ｍＷ／ｃｍ＾２毫米波，在小鼠怀孕６ｄ￣１５ｄ时进行２ｈ／ｄ辐射，用电迷宫对成年子鼠进行学习记忆功能测试，用受体放射配基结合分析（ＲＢＡ）对子鼠脑胆碱能－Ｍ受体（Ｍ－Ｒ）进行测定。结果显示：３７．４，４２．２ＧＨｚ毫米波，≥５ｍＷ／ｃｍ＾２辐射可导致     

铝酞菁D及其与黄夹甙或维拉帕米合用对肿瘤细胞的光动力治疗作用

目的 研究新型光敏剂铝酞菁Ｄ（ＡｌＰＣ－Ｄ）单用及与黄夹甙或维拉帕米合用对体外培养肿瘤细胞的光动力学治疗作用。方法 细胞经药物作用２ｈ后，用红光辐照一定时间，继续培养２４ｈ，台盼蓝排染法测定药物对肿瘤细胞的抑制率。结果 ２．５￣１０．０μｇ／ｍｌ ＡｌＰＣ－Ｄ配以５．４或１０．８Ｊ／ｃｍ＾２的红光射照，能显著杀伤Ｋ５６２和ＳＧＣ７９０１细胞，并有剂量依赖性。０．００１和０．０１μｇ／ｍｌ黄夹甙或单     

微波对大鼠肝、血清中MDA含量及SOD活性影响

０　引言　微波辐照已经成为人们生活和劳动的重要环境因素之一,也是军队军事作业和战场作战可能造成人员健康和战斗力伤害的一种环境物理因素．我们对微波辐照后大鼠肝和血清中丙二醛（ＭＤＡ）含量、超氧化物歧化酶（ＳＯＤ）活性的影响进行了初步观察．１　材料和方法１．１　动物分组　实验动物由取得合格证的上海实验动物中心提供的ＳＤ雄性健康大鼠２４只,体质量（２００±２０）ｇ,经１ｗｋ适应性饲养后,随机分为６１ｍＷ／ｃｍ２组、３０ｍＷ／ｃｍ２组及对照组,每组动物８只．１．２　微波辐射实验参数　微波源输出功率１５２…     

铝酞菁D及其与黄夹甙或维拉帕米合用对肿瘤细胞的光动力治疗作用

目的研究新型光敏剂铝酞菁Ｄ（ＡｌＰＣ－Ｄ）单用及与黄夹甙或维拉帕米合用对体外培养肿瘤细胞的光动力治疗作用。方法细胞经药物作用２ｈ后，用红光辐照一定时间，继续培养２４ｈ，台盼蓝排染法测定药物对肿瘤细胞的抑制率。结果２．５～１０．０μｇ／ｍｌＡｌＰＣ－Ｄ配以５．４或１０．８Ｊ／ｃｍ２的红光射照，能显著杀伤Ｋ５６２和ＳＧＣ７９０１细胞，并有剂量依赖性。０．００１和０．０１μｇ／ｍｌ黄夹甙或单用无效剂量的维拉帕米（５μｇ／ｍｌ）与ＡｌＰＣ－Ｄ合用，能增强后者对Ｋ５６２细胞的光动力作用。结论ＡｌＰＣ－Ｄ对体外培养的肿瘤细胞具有光动力治疗作用，黄夹甙和维拉帕米能增强其作用。     

高密度脂蛋白氧化修饰对大鼠巨噬细胞胆固醇流出的影响及其…

研究高密度脂蛋白－３（ＨＤＬ３）氧化修饰对其介导大鼠腹腔巨噬细胞胆固醇（ｃｈ）流出功能影响及细胞ｃｈ流出机制。ＨＤＬ３和其组分载脂蛋白Ａ－Ｉ（ＡｏｏＡ－Ｉ）经Ｃｕ＾２＋氧化修饰后分别与巨噬细胞（ＭΦ）一起培养，测定细胞ｃｈ流出量。结果：牛血清白蛋白（对照组）介导３．９８％细胞ｃｈ流出，ＨＤＬ３和氧化ＨＤＬ３（Ｏｘ－ＨＤＬ３）组分别为４１．７８％和１７．４３％，ＡｐｏＡ－Ｉ和Ｏｘ－ＡｐｏＡ－Ｉ分别为     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.