HLA-DRB1、DQB1基因与汉族哮喘的相关性研究

探讨 HL A- DRB1、DQB1位点基因在中国汉族哮喘家系中与哮喘的相关性。用序列特异性引物 -聚合酶链反应 (PCR- SSP)方法 ,对 10 1例哮喘家系成员和 6 0例正常对照者进行了 HL A- DRB1、DQB1等位基因的分型 ,并分析了 DRB1、DQB1基因在两组中的分布。结果示 ,与正常对照组比较 ,哮喘患者组 DRB1* 15等位基因频率 (2 0 .93% )较正常对照组 (6 .6 7% )明显增高 (χ2 =10 .95 ,P<0 .0 5 ) ;DQB1* 0 6 0 1等位基因频率在哮喘患者组(31.4 0 % )较正常对照组 (7.5 % )明显增高 (χ2 =2 3.0 8,Pc<0 .0 1)。同时发现 HL A- DRB1* 0 7等位基因频率在对屋尘螨抗原皮试阳性家系成员中 (2 7.4 2 % )较家系中皮试阴性者 (5 .36 % )显著增高 (χ2 =10 .83,Pc<0 .0 5 )。HL A- DRB1* 15和 DQB1* 0 6 0 1可能是汉族哮喘的遗传等位易感基因 ,HL A- DRB1* 0 7在限定对屋尘螨抗原特异性 Ig E反应过程中起重要作用     

HLA—DRB1、DQB1基因多态性与流行性出血热的相关性

目的从基因水平探讨HLA—DRB1、DQB1等位基因多态性与流行性出血热的相关性,以阐述其免疫遗传学特征。方法应用序列特异性引物聚合酶链反应技术检测流行性出血热患者和正常对照组各50例的HLA—DRB1、DQB1等位基因。结果流行性出血热患者与正常对照组比较,HLA—DRB1＊16等位基因分布频率明显增高（Pc=0．0106）,两组间其余HLA-DRB1、-DQB1等位基因分布频率差异无统计学意义（Pc〉0．05）。结论HLA-DRB1＊16等位基因与流行性出血热呈正相关,可能为流行性出血热易感基因之一。     

上海地区Ⅰ型自身免疫性肝炎与HLA-DRB1等位基因的相关性研究

目的 分析HLA－DRB1等位基因与上海地区I型自身免疫性肝炎（AIH）的相关性，探讨AIH的遗传易感背景。方法 采用序列特异性多聚酶链反应（PCR－SSP），对32例I型AIH患者和48例健康对照者进行HLA－DRB1等位基因及有关基因亚型的分析。结果 HLA－DR4基因频率在I型AIH患者中较健康对照组显著增高[46.9%与20.8%；相对危险度（RR）＝3．35，χ^2=5.99,P=0.014]。其他等位基因在两组间差异无显著性。进一步对HLA－DR4等位基因亚型的分析表明，I型AIH患者组DRB1^*0405的基因频率较健康对照组有增加趋势（21．9％与6．3％，χ^2=4.23,P=0.04，但Pc=0.08)。HLA－DRβ分子的第3等位基因高变区第71位精氨酸残基的频率在I型AHI患者中显著增高（46.9%与18.8%,χ^2=7.14,P=0.008)。结论 上海地区I型AIH的发病与HLA－DR4以及HLA－DRB1第3高变区DR7位精氨酸残基相关。     

山东沿海地区碘营养状况和HLA等位基因DRB1*0301、DQB1*0602对自身免疫性甲状腺疾病发病的影响

目的　将山东沿海地区碘营养状况和HLA DRB1 0 3 0 1、DQB1 0 60 2等位基因等因素结合起来分析 ,观察它们对自身免疫性甲状腺疾病 (AITDs)发病的影响。方法　应用多聚酶链反应 序列特异性引物 (PCR SSP)技术 ,对已确诊的Graves病 (GD) 90例 ,桥本甲状腺炎 (HT) 43例和正常对照 90例 ,扩增其HLA等位基因DRB1 0 3 0 1、DQB1 0 60 2的目的DNA片段。同时采用国家标准化方法砷铈催化分光光度法测定不同人群尿碘浓度。结果　山东沿海地区GD和HT患者尿碘中位数显著高于对照组 (P <0 .0 5 )。GD和HT组尿碘浓度高于 3 0 0 μg/L者分布频率均显著高于对照组 (P <0 .0 5 )。DRB1 0 3 0 1( -)GD、HT患者尿碘中位数显著高于DRB1 0 3 0 1( -)的对照组 (P<0 .0 5 ) ;DQB1 0 60 2 ( +)GD、HT患者尿碘中位数显著高于DQB1 0 60 2 ( +)的对照组 (P <0 .0 5 )。多因素logistic回归分析发现尿碘浓度对数、DRB1 0 3 0 1与GD、HT的发病呈正相关 ,DQB1 0 60 2与GD、HT的发病呈负相关。结论　该地区GD、HT患者的碘摄入量偏高 ;碘和HLA等位基因DRB1 0 3 0 1、DQB1 0 60 2共同影响GD、HT的发病     

HLAⅡ类基因与狼疮性器官系统损害的相关性研究

目的　分析HLA Ⅱ类基因与狼疮性器官、系统损害的相关性 ,探讨系统性红斑狼疮(SLE)从基因到临床表达的发病机制。方法　应用PCR SSOPH技术检测 113例确诊SLE病人的DR、DQA1、DQB1等位基因。结果　浆膜炎与DQB1 0 6 0 1正相关 ;神经精神症状与DRB1 15 0 1正相关 ;血肌酐增高与DQB1 0 30 2、DQB1 0 40 1正相关 ;血尿素氮增高与DQB1 0 2 0 1负相关 ;蛋白尿与DQB1 0 30 1、DRB3 0 2 0 2、DRB3 0 30 1、DRB1 110 1负相关 ;血尿与DQB1 0 30 1、DRB3 0 30 1负相关 ;管型尿与DRB1 0 30 2正相关 ;贫血和白细胞减少分别与DQB1 0 2 0 1正相关 ;血小板减少与DRB1 15 0 2和DRB1 12 0 2正相关 ;淋巴细胞减少与DRB1 0 30 2正相关 ;C3降低与DQB1 0 30 3正相关 ,与DQB1 0 30 1负相关 ;狼疮细胞阳性与DRB1 15 0 1负相关。结论　多个HLA Ⅱ类等位基因和SLE的临床表达有相关性 ,国人的SLE遗传背景与其他人种有所不同。     

抗SSA和SSB抗体与HLA-Ⅱ基因的相关性分析

目的：探讨云南汉族系统性红斑狼疮（SLE）患者抗SSA和SSB抗体与HLA－DRB1、DQA1、DQB1等位基因及单体型的相关性。方法：采用多聚酶链反应－序列特异性引物（PCR－SSP）技术对63例云南汉族SLE患者和54名同民族健康对照进行DRB1、DQA1、DQB1基因分型。结果：云南汉族SLE患者中DR15（P＜0．01）、DR16（P＜0．05）、DQA1＊0102（P＜0．05）、DQA1＊0103（P＜0．01）、DQB1＊0601（P＜0．05）等位基因频率明显增高；抗SSA和SSB抗性阳性的SLE病人中DQA1＊0103频率均显著增高（P＝0．042，P＝0．006）；抗SSB抗体阳性的SLE患者中的DQA1＊0501 频率均显著增高（P＝0．009）。结论：云南汉族SLE 抗SSA和SSB抗体的产生与DQA1＊0103等位基因相关；抗SSB抗体的产生还与DQA1＊0501相关。     

1型糖尿病HLA-DPB1、DQB1基因与其自身抗体相关性研究

目的：研究HLA－DPB1、DQB1基因在中国汉族1型糖尿病患者中的多态性分布及其与自身抗体的相关性。方法：采用基于核酸序列测定的基因分型技术,确定68例1型糖尿病患者及50例对照的HLA－DPB1、DQB1基因型。用酶联免疫吸附法测定自身抗体[谷氨酸脱羧酶抗体（GADA）,胰岛细胞抗体（ICA）,胰岛素自身抗体（IAA）]。结果：在1型糖尿病患者中,DPB1＊0402和DQB1＊0301等位基因频率显著低于对照,DPB1＊2201及DQB1＊0201,＊0303及＊0604等位基因频率显著高于对照。在等位基因为DPB1＊0402的患者IAA阳性率显著低于此等位基因阴性者,在等位基因为DQB1＊0201的患者GADA阳性率显著高于此等位基因阴性者。结论：在中国汉族人群中,DPB1＊2201,DQB1＊0201,＊0303,＊0604是1型糖尿病易感性等位基因,DPB1,＊0402及DQB1＊0302是该病保护性等位基因,DPB1＊0402与IAA呈负相关,DQB1＊0201与GADA呈正相关。     

30例重症肌无力患者人类白细胞抗原-Ⅱ基因多态性测定及分析

目的 探讨人类白细胞抗原（HLA）-Ⅱ基因多态性在贵州汉族人群重症肌无力（MG）发病中的作用。方法选择本地区汉族人群中30例MG患者（MG组）、36例神经内科非自身免疫性疾病患者（疾病对照组）及30例健康查体者（正常对照组）,采用PCR-序列特异性引物（PCR-SSP）技术进行HLA-Ⅱ类基因DRB1、DQB1位点分型,并对其与Mc的相关性进行分析。结果MG组HLA-DRB1＊0901和DQB1＊0303等位基因频率显著高于其他两组（P〈0．05）,DQB1＊0601等位基因频率显著低于其他两组（P〈0．05）。结论HIA-DRB1＊0901及DQB1＊0303可能为MC的易感基因,而HLA—DQ＊0601为保护基因。     

儿童期1型糖尿病与HLA—DQB1等位基因的关联研究

目的 研究哈尔滨市儿童期1型糖尿病与HLA－DQB1等位基因的关联关系。方法 选取49例0－14岁发病的1型糖尿病患者和75名健康儿童对照,运用PCR－SSOP技术对部分HLA－DQB1等位基因进行了分型。结果 发现在HLA－DQB1位点上,病例组的DQB1＊0201、＊0303、＊0401频率显著高于对照组,而DQB1＊0301、DQB1＊0501和＊0601频率是显著下降的。结论 研究提示：HLA－DQB1位点的＊0201、＊0303和＊0401对儿童期1型糖尿病具有强易感性,而DQB1＊0301、＊0501和＊0601具有保护性。但未证实DQB1＊0602具有保护作用。     

中国人群1型糖尿病HLA-DQ基因多态性的Meta分析

目的　综合评价中国人群HLA DQ基因多态性与 1型糖尿病 (DM)的关联性。方法　以 1型DM组和健康对照组的各HLA DQ等位基因频数(基因型频数、单倍型频数 )分布的OR值为统计量,全面检索相关文献;应用Meta分析软件包REVMAN4. 2,在基因分型水平上,对各研究的结果进行一致性检验和数据合并,并评估发表偏倚。结果　等位基因DQA1* 0301、DQA1* 0501、DQB1* 0201、DQB1* 0303、DQB1* 0401和DQB1* 0604是中国人群 1型DM的危险基因 (均P<0. 05), 他们的合并OR值分别为2. 83、2. 90、4. 17、1. 65、2. 00和 3. 00;基因型 (或单倍型 )DQA1* 0301 /DQB1* 0201、DQA1* 0301 /DQB1*0302、DQA1* 0501 /DQB1* 0201、DQA1* 0301 /DQB1* 0201 /DRB1* 0301和DQB1* 0302 /DRB1* 0405是中国人群 1型DM的危险基因型(或单倍型,均P<0. 05),他们的合并OR值分别为 8. 95、3. 09、6. 01、6. 57和 14. 85。而等位基因DQA1* 0101、DQA1* 0102、DQA1* 0103、DQA1* 0104、DQA1* 0201、DQA1* 0401、DQA1* 0601、DQB1* 0301、DQB1* 0501、DQB1* 0503、DQB1* 0601和DQB1* 0602是中国人群 1型DM的保护等位基因(均P<0. 05),他们的合并OR值分别为 0. 47、0. 38、0. 21、0. 07、0. 44、0. 39、0. 44、0. 19、0. 33、0. 32、0. 42和 0. 28; 基因型     

The association between the genetic polymorphism of HLA-DQA1, DQB1, and DRB1 and serum alanine aminotransferase levels in chronic hepatitis C in the Chinese population

Background and Aim:  To investigate a possible association between HLA genes with serum alanine aminotransferase (ALT) levels and evaluate whether the HLA-DQA1, DQB1, and DRB1 genes could influence the development of liver damage in chronic hepatitis C.
Methods:  A total of 145 patients with chronic hepatitis C virus (HCV) infection (36 patients with persistently normal ALT values; 109 patients with elevated ALT levels) and 160 uninfected healthy controls were examined for HLA-DQA1, DQB1, and DRB1 molecules by using polymerase chain reaction–sequencing based typing (PCR-SBT).
Results:  Among the patients chronically infected with HCV, the frequencies of DQA1*0501, DQB1*0301, and DRB1*0401 alleles were significantly increased in the normal ALT group compared with those with abnormal ALT levels, whereas that of DQB1*0201 was significantly lower. As compared to uninfected healthy controls, DQA1*0501, DQB1*0301, and DRB1*0401 allele frequencies were also statistically higher in the normal ALT group, whereas that of DQB1*0201 was the inverse. The haplotype frequencies of DQA1*0301-DQB1*0301, DQA1*0501-DQB1*0301, and DRB1*1101-DQB1*0301 were found to be significantly higher in the normal ALT group. Multivariate logistic regression indicated that female sex, and the DQB1*0301 allele and DRB1*0401 allele were independently associated with normal ALT values, whereas DQB1*0201 allele was the inverse.
Conclusions:  These results suggest that particular HLA alleles may have an influence on the serum ALT level of chronic HCV infection as a host genetic factor in the Chinese population. The DQA1*0501, DQB1*0301, and DRB1*0401 alleles, and the DQA1*0301-DQB1*0301, DQA1*0501-DQB1*0301, and DRB1*1101-DQB1*0301 haplotypes seem to be associated with low hepatitis activity; whereas DQB1*0201 allele is closely correlated with the progression of liver injury in chronic HCV infection.     

HLA-DRB1 allele polymorphisms in genetic susceptibility to esophageal carcinoma

AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal carcinoma in Han Chinese in Hubei Province. METHODS: HLA-DRB1 allele polymorphisms were typed by polymerase chain reaction with sequence-specific primers(PCR-SSP) in 42 unrelated patients with esophageal cancer and 136 unrelated normal control subjects and the associated HLA-DRB1 allele was measured by nucleotide sequence analysis with PCR.SAS software was used in statistics. RESULTS: Allele frequency (AF) of HLA-DRB1*0901 was significantly higher in esophageal carcinoma patients than that in the normal controls (0.2500 vs 0.1397, P=0.028, the odds ratio 2.053, etiologic fraction 0.1282). After analyzed the allele nucleotide sequence of HLA-DRB1*0901 which approachs to the corresponded exon 2 sequence of the allele in genebank. There was no association between patients and controls in the rested HLA-DRB1 alleles. CONCLUSION: HLA-DRB1*0901 allele is more common in the patients with esophageal carcinoma than in the healthy controls, which is positively associated with the patients of Hubei Han Chinese. Individuals carrying HLA-DRB1*0901 may be susceptible to esophageal carcinoma.     

HLA class II genotypes associated with chronic hepatitis C virus infection and response to alpha-interferon treatment in Poland

AIMS/BACKGROUND: Recent evidence suggests that spontaneous clearance of hepatitis C virus (HCV) may be associated with the HLA DQB1*0301 allele but there is still some debate over the role of other alleles and HLA haplotypes in HCV infection. As this may best be resolved by studying genetically different populations, we have investigated HLA class II-encoded susceptibility and resistance to HCV infection in a relatively sedentary population of patients from northwestern Poland. METHODS: The distributions of HLA class II DRB1, DQA1, DQB1 and DPB1 alleles were determined by standard PCR-protocol in 129 unrelated patients with chronic hepatitis C (anti-HCV and HCV-RNA positive) and 103 healthy unrelated racially-matched control subjects. Fifty-five patients were treated with alpha-interferon (5 MIU thrice weekly for 6 months) out of whom 29 showed a complete response and 26 were non-responders. RESULTS: A significantly reduced frequency of the DQB1*0301 allele in the patients was observed (24.0% vs. 38.8%; p=0.015). Additionally, two different DR-DQ haplotypes were found to be associated with chronic HCV infection: DRB1*1501-DQA1*01-DQB1*0602 (24.0% vs. 12.6%; p= 0.027) and DRB1*0701-DQA1*0201-DQB1*02 (31.8 vs. 12.6%; p=0.0006), the latter difference being most pronounced in those patients who responded to alpha-interferon treatment (41.4% vs. 12.6%; p=0.00048). CONCLUSIONS: The results confirm the negative association between chronic HCV and DQB1*0301 and identify two novel genetic associations. In particular, the DRB1*0701-DQA1*0201-DQB1*02 haplotype is associated with both chronic infection and response to alpha-interferon. Interestingly, the same haplotype is reportedly associated with non-response to hepatitis B vaccination.     

HLA class II genotypes associated with chronic hepatitis C virus infection and response to α‐interferon treatment in Poland

Abstract: Aims/Background: Recent evidence suggests that spontaneous clearance of hepatitis C virus (HCV) may be associated with the HLA DQB1*0301 allele but there is still some debate over the role of other alleles and HLA haplotypes in HCV infection. As this may best be resolved by studying genetically different populations, we have investigated HLA class II‐encoded susceptibility and resistance to HCV infection in a relatively sedentary population of patients from northwestern Poland. Methods: The distributions of HLA class II DRB1, DQA1, DQB1 and DPB1 alleles were determined by standard PCR‐protocol in 129 unrelated patients with chronic hepatitis C (anti‐HCV and HCV‐RNA positive) and 103 healthy unrelated racially‐matched control subjects. Fifty‐five patients were treated with α‐interferon (5 MIU thrice weekly for 6 months) out of whom 29 showed a complete response and 26 were non‐responders. Results: A significantly reduced frequency of the DQB1*0301 allele in the patients was observed (24.0% vs. 38.8%; p=0.015). Additionally, two different DR‐DQ haplotypes were found to be associated with chronic HCV infection: DRB1*1501‐DQA1*01‐DQB1*0602 (24.0% vs. 12.6%; p=0.027) and DRB1*0701‐DQA1*0201‐DQB1*02 (31.8 vs. 12.6%; p=0.0006), the latter difference being most pronounced in those patients who responded to α‐interferon treatment (41.4% vs. 12.6%; p=0.00048). Conclusions: The results confirm the negative association between chronic HCV and DQB1*0301 and identify two novel genetic associations. In particular, the DRB1*0701‐DQA1*0201‐DQB1*02 haplotype is associated with both chronic infection and response to α‐interferon. Interestingly, the same haplotype is reportedly associated with non‐response to hepatitis B vaccination.     

Human leukocyte antigen class Ⅱ DQB1*0301, DRB1*1101 alleles and spontaneous clearance of hepatitis C virus infection: A meta-analysis

AIM: To assess the associations of human leukocyte antigen (HLA) class Ⅱ DQB1*0301 and/or DRB1*1101 allele with spontaneous hepatitis C virus (HCV) clearance by meta-analysis of individual dataset from all studies published till date.METHODS: To clarify the impact of HLA class Ⅱ polymorphisms on viral clearance, we performed a metaanalysis of the published data from 11 studies comparing the frequencies of DQB1*0301 and DRB1*1101 alleles in individuals with spontaneous resolution to those with persistent infection. As we identified the heterogeneity between studies, summary statistical data were calculated based on a random-effect model.RESULTS: Meta-analyses yielded summary estimatesodds ratio (OR) of 2.36 [95%CI (1.62, 3.43), P<0.00001]and 2.02 [95%CI (1.56, 2.62), P<0.00001] for the effects of DQB1*0301 and DRB1*1101 alleles on spontaneous clearance of HCV, respectively.CONCLUSION: These results support the hypothesis that specific HLA class Ⅱ alleles might influence the susceptibility or resistance to persistent HCV infection.Both DQB1*0301 and DRB1*1101 are protective alleles and present HCV epitopes more effectively to CD4+T lymphocytes than others, and subjects with these two alleles are at a lower risk of developing chronic HCV infection. Large, multi-ethnic confirmatory and welldesigned studies are needed to determine the host genetic determinants of HCV infection.     

HLA-DRB3*0101 is associated with Graves' disease in Jamaicans

OBJECTIVES: Graves' disease is associated with different human leucocyte antigen (HLA) genes in different populations. This studywasdesigned to examinethe HLA class II associations with Graves' disease in Jamaicans. PATIENTS: One hundred and six Jamaicans with Graves' disease and 104 controls. DESIGN: Oligotyping for HLA-DRB1, DRB3, DQA1 and DQB1 alleles was performed using the polymerase chain reaction sequence specific oligonucleotide probe (PCR-SSOP) technique. RESULTS The frequency of HLA-DRB3 *0101 was increased significantly in the patients compared to controls (38.7% vs. 19.2%; RR = 2.72; Pc < 0.015). The protective alleles for Graves' disease were DRB1 *0901 (0.9% vs. 20.2%; RR = 0.04; Pc < 0.001), DRB1*1001 (0.0% vs. 11%; RR = 0.0%; Pc < 0.01) and DRB4 *0101 (0.0% vs. 12.5%; RR = 0.0; Pc < 0.05). A high female to male ratio of Graves' disease, 25 :1, was observed. Other associated autoimmune diseases were rare and no significant HLA class II associations were found with clinical markers of disease. CONCLUSIONS: Jamaican patients with Graves' disease share the DRB3 *0101 susceptible allele and the DRB4 *01 protective allele but not the susceptible haplotype DRB1 *0301, DRB3*0101, DQA1*0501 with Caucasians.     

Human leukocyte antigen class Ⅱ DQB1＊0301, DRB1＊1101 alleles and spontaneous clearance of hepatitis C virus infection： A metaanalysis

AIM: To assess the associations of human leukocyte antigen (HLA) class II DQB1*0301 and/or DRB1*1101 allele with spontaneous hepatitis C virus (HCV) clearance by meta-analysis of individual dataset from all studies published till date. METHODS: To clarify the impact of HLA class II polymorphisms on viral clearance, we performed a meta-analysis of the published data from 11 studies comparing the frequencies of DQB1*0301 and DRB1*1101 alleles in individuals with spontaneous resolution to those with persistent infection. As we identified the heterogeneity between studies, summary statistical data were calculated based on a random-effect model. RESULTS: Meta-analyses yielded summary estimates-odds ratio (OR) of 2.36 [95%CI (1.62, 3.43), P<0.00001] and 2.02 [95%CI (1.56, 2.62), P<0.00001] for the effects of DQB1*0301 and DRB1*1101 alleles on spontaneous clearance of HCV, respectively. CONCLUSION: These results support the hypothesis that specific HLA class II alleles might influence the susceptibility or resistance to persistent HCV infection. Both DQB1*0301 and DRB1*1101 are protective alleles and present HCV epitopes more effectively to CD4+T lymphocytes than others, and subjects with these two alleles are at a lower risk of developing chronic HCV infection. Large, multi-ethnic confirmatory and well-designed studies are needed to determine the host genetic determinants of HCV infection.     

HLA DPB1*0201 gene confers disease susceptibility in japanese with childhood onset type I diabetes, independent of HLA-DR and DQ genotypes

HLA is an important etiologic genetic factor in Type I diabetes and specific HLA-class II genes are closely related to the onset of the disease. Many differences in the patterns of susceptible and resistant DRB1, DQA1, and DQB1 genes have been observed among various ethnic groups. We have previously shown that DRB1*0405, DRB1*0901 and DQA1*0301-DQB1*0302 were the major susceptible alleles or haplotype to Type I diabetes while DR-DQ haplotype studies suggested the important role of DR and DQ alleles in susceptibility and resistance in Japanese patients. Based on the analysis of 90 Japanese patients with childhood onset Type I diabetes and 136 unrelated healthy Japanese controls by polymerase chain reaction-restriction fragment polymorphism method (PCR-RFLP), we report here the association of Type I diabetes with DPB1*0201 (relative risk = 2.29; Pc = 0.027) in this population. Comparison of linkage disequilibrium patterns between patients and controls showed that the significantly high prevalence of DPB1*0201 among patients cannot be attributed simply to linkage disequilibrium with susceptible DRB1 alleles and DQA1-DQB1 haplotypes. Our results suggest that in addition to alleles at the DRB1, DQA1, DQB1 loci, polymorphism at DPB1 locus also influences the risk of Type I diabetes.     

Human leukocyte antigen class II and cervical cancer risk: a population-based study

The critical role of the human leukocyte antigen (HLA) system in presenting peptides to antigen-specific T cell receptors may explain why only some human papillomavirus (HPV)-infected women progress to cervical cancer. HLA class II DRB1 and DQB1 genes were examined in 315 women with invasive squamous cell cervical cancer (SCC) and 381 control subjects. Increased risks of SCC were associated with DRB1*1001, DRB1*1101, and DQB1*0301, and decreased risks were associated with DRB1*0301 and DRB1*13. Of squamous cell tumors, those containing HPV-16 were different from those not containing HPV-16 for 3 alleles: DRB1*0401, DRB1*07, and DQB1*06. Increased risks of SCC were associated with DRB1*0401-DQB1*0301 (odds ratio [OR], 1.7; 95% confidence interval [CI], 1.1-2.7) and DRB1*1101-DQB1*0301 (OR, 2.5; 95% CI, 1.4-4.5), and decreased risks were associated with DRB1*0301-DQB1*02 (OR, 0.7; 95% CI, 0.5-1.0) and DRB1*13-DQB1*06 (OR, 0.6; 95% CI, 0.4-0.9) haplotypes. These results add to the evidence that certain HLA class II alleles or allele combinations, or genes linked to them, make some women more susceptible to SCC.