黄芪多糖对健康体检者淋巴细胞亚群的调节作用

目的：探讨黄芪多糖对健康成人免疫力的调节作用。方法：研究对象为本实验室26名工作人员，为自愿受试者，纳入标准：①健康体检合格。②年龄21～68岁。实验时间为2004—01—12／02—12，每日早晚各2次服用黄芪多糖，每次一袋5g，2周1个疗程，总疗程共4周，分别比较用药前及用药后第2周、第4周免疫5项指标及外周血白细胞检测。免疫5项指标用Simultest TM IMK-Lymphocyte Kit荧光抗体对血液进行双标染色，以FACS Calibur流式细胞仪进行分析，SimulSET获取数据并分析结果。结果：服药2，4周后与服药前相比，总淋巴细胞水平有明显增高趋势，差异有显著性意义(P&;lt;0．05)，T辅助细胞和T抑制细胞(CD3，CD4／CD3，CD Ratio)比值增高；T抑制细胞(CD3+，CD8+)水平降低，4周后变化更加明显(P&;lt;0．01)，NK细胞活性有增高趋势，但差异无显著性，其他指标无明显变化。并且服药后肌酸激酶、乳酸脱氢酶、肌酸激酶同功酶及天冬氨酸氨基转移酶、丙氨酸氨基转移酶等酶的水平没有明显变化。结论：黄芪多糖通过增高T辅助细胞和T抑制细胞(CD3，CD4／CD3，CD Ratio)比值及总淋巴细胞、NK细胞活性来提高人体免疫水平。未造成心脏、肝脏功能的改变。     

黄芪多糖对健康体检者淋巴细胞亚群的调节作用

目的:探讨黄芪多糖对健康成人免疫力的调节作用。方法:研究对象为本实验室26名工作人员,为自愿受试者,纳入标准:①健康体检合格。②年龄21～68岁。实验时间为2004-01-12/02-12,每日早晚各2次服用黄芪多糖,每次一袋5g,2周1个疗程,总疗程共4周,分别比较用药前及用药后第2周、第4周免疫5项指标及外周血白细胞检测。免疫5项指标用SimultestTMIMK-LymphocyteKit荧光抗体对血液进行双标染色,以FACSCalibur流式细胞仪进行分析,SimulSET获取数据并分析结果。结果:服药2,4周后与服药前相比,总淋巴细胞水平有明显增高趋势,差异有显著性意义(P<0.05),T辅助细胞和T抑制细胞(CD3,CD4/CD3,CDRatio)比值增高;T抑制细胞(CD3+,CD8+)水平降低,4周后变化更加明显(P<0.01),NK细胞活性有增高趋势,但差异无显著性,其他指标无明显变化。并且服药后肌酸激酶、乳酸脱氢酶、肌酸激酶同功酶及天冬氨酸氨基转移酶、丙氨酸氨基转移酶等酶的水平没有明显变化。结论:黄芪多糖通过增高T辅助细胞和T抑制细胞(CD3,CD4/CD3,CDRatio)比值及总淋巴细胞、NK细胞活性来提高人体免疫水平。未造成心脏、肝脏功能的改变。     

肿瘤患者外周血T淋巴细胞亚群的变化及临床价值分析

目的 分析肿瘤患者外周血T淋巴细胞亚群的变化及临床价值。方法 选取治疗的肿瘤组患者60例,另选取同期健康体检人员60例作为对照组。抽取所有研究人员外周静脉血2ml,采用流式细胞仪法对T淋巴细胞进行测定;另抽取肘静脉血3ml采用免疫散射比浊法测定血清中IgG、IgA、IgM含量。结果 对比各组间治疗前后外周血T淋巴细胞亚群检测结果及IgG、IgA、IgM含量。治疗后肺癌、食道癌及其他肿瘤组CD3+、CD4+及CD4+/CD8+水平均高于治疗前,CD8+水平低于治疗前,差异有统计学意义(P0.05);与对照组相比,肿瘤组治疗前后CD3+、CD4+及CD4+/CD8+水平均较低,CD8+水平均较高,差异有统计学意义(P0.05);肿瘤组IgG、IgA、IgM含量均高于对照组,差异无统计学意义(P0.05)。结论 对肿瘤患者实施外周血T淋巴细胞亚群各指标测定,有助于及时准确地掌握患者免疫功能变化,从而为临床诊治提供参考依据,更好地明确治疗方案,对评估治疗效果及预后具有重要作用,值得推广应用。     

华蟾素注射液体外对小鼠脾淋巴细胞功能影响的研究

目的观察华蟾素注射液对小鼠脾淋巴细胞增殖、活化的影响,探讨其对免疫系统调节的作用,为阐明其免疫作用机制提供药理学依据。方法无菌分离小鼠脾淋巴细胞,制备淋巴细胞悬液,按临床给药量设置华蟾素注射液不同浓度组给药。MTT法分别检测刀豆蛋白A(Con A)刺激和脂多糖(LPS)刺激的小鼠脾淋巴细胞增殖;用华蟾素注射液预孵育淋巴细胞6 h,分别加入Con A或LPS诱导淋巴细胞活化,利用流式细胞仪通过Aimplex对细胞上清液进行GM-CSF、TNF-α,IFN-γ,IL-1α,IL-2,IL-6,IL-10细胞因子检测,评价其免疫效果。结果华蟾素注射液7.82mg/ml、15.63 mg/ml和31.25 mg/ml浓度组与Con A协同刺激淋巴细胞增殖。1.96 mg/ml和62.50 mg/ml浓度组与LPS协同抑制细胞增殖。华蟾素注射液可促进TNF-α和IL-6分泌,一定程度抑制IFN-γ和GM-CSF分泌,与不同刺激剂作用可不同程度促进或抑制IL-2、IL-1α和IL-10的分泌。结论华蟾素注射液可一定程度促进B淋巴细胞增殖,但对体液免疫的作用效果和作用机制还有待进一步研究。     

血必净注射液对严重创伤患者外周血T淋巴细胞亚群变化的干预研究

目的 探讨血必净注射液对严重创伤患者外周血T淋巴细胞亚群变化的影响及其临床意义.方法 将33例严重刨伤患者随机分为对照组(16例)和血必净治疗组(17例).对照组患者给予常规救治,血必净治疗组患者则在常规救治的基础上早期加用血必净注射液治疗.采用双抗体标记流式细胞分析技术测定患者人院时、伤后3 d和5 d的外周血T淋巴细胞及其亚群CD4 、CD8 的百分率及CD4 /CD8 比值.结果 两组严重创伤患者伤后3 d和5 d时,外周血CD4 和CD4 /CD8 比值均较人院时明显下降(CD4 对照组为(32.40±6.02)%、(34.19±5.63)%比(45.09±6.57)%,治疗组为(34.30±5.46)%、(38.55±6.47)%比(46.10±6.02)%;CD4 /CD86 比值对照组为1.11±0.20、1.15±0.20比1.60±0.20,治疗组为1.20±0.22、1.32±0.24比1.66±0.24,P均＜0.01].伤后5 d时,血必净治疗组患者的外周血CD4 和CD4 /CD8 比值明显回升[(38.55±6.47)%、1.32±0.243,与对照组[(34.19±5.63)%、1.15±0.20]比较差异有统计学意义(P均＜0.05).结论 严重创伤患者在常规救治的基础上早期使用血必净注射液,能较快纠正T细胞辅助/抑制免疫炎症调节网络的紊乱或抑制,有助于维持免疫炎症调节功能的平衡.     

肺癌患者外周血T淋巴细胞亚群的变化

化疗的同时附加免疫支持药物是临床医生针对恶性肿瘤患者采取的一个生物治疗手段。就化疗联合免疫支持治疗对肺癌患者机体免疫功能的影响进行研究，报告如下。     

他可莫司对肝移植受者外周血T淋巴细胞亚群的影响及临床意义

目的探讨他可莫司对肝移植后受者外周血T淋巴细胞亚型的影响及临床意义。方法采用微粒子酶免疫分析法进行他可莫司全血谷浓度测定，监测16例肝移植受者在第1周、第2周、第1个月-第4个月及第6个月的血药浓度。全血样本同时用血细胞分析仪测定血常规，流式细胞仪检测外周血T淋巴细胞亚群。结果他可莫司平均血药浓度基本在推荐治疗窗范围内。他可莫司显著减少肝移植受者外周血CD3^＋CD4^CD8^-细胞百分比，但减少的程度并不受他可莫司血药浓度影响。接受他可莫司治疗后，肝移植早期受者外周血淋巴细胞总数和CD3^＋T淋巴细胞的绝对计数都显著减少。结论他可莫司主要抑制肝移植受者CD3^＋CD4^＋CD8^-T淋巴细胞亚群，但其全血谷浓度并不反应T淋巴细胞亚群受影响的程度。提示应在肝移植早期监测他可莫司全血谷浓度和血常规及T淋巴细胞亚群的三项组合检验，为实施个体化检验和临床用药治疗方案调整提供方便和帮助。     

高强度超声介入治疗对宫颈癌小鼠模型肿瘤增殖、免疫功能及脾细胞Th1/Th2亚群的影响

目的 探讨高强度超声介入治疗对宫颈癌小鼠模型肿瘤增殖、免疫功能及脾细胞Th1/Th2亚群的影响。方法 42只宫颈癌小鼠按随机数字表法分为3组-模型组、超声1组与超声2组,每组14只。超声1组与超声2组均给予进行局部高强度超声治疗,超声1组的单点单次治疗时间为10 s,超声2组的单点单次治疗时间为20 s,共治疗15次;模型组不给予超声治疗。所有小鼠均治疗观察4周,记录3组小鼠的体重、肿瘤体积、肿瘤增殖、免疫功能及脾细胞Th1/Th2亚群变化情况。结果 超声1组与超声2组治疗第2周与第4周的小鼠体重均高于模型组,肿瘤体积均小于模型组,超声2组较超声1组变化更显著,差异均有统计学意义(P<0.05)。超声1组与超声2组治疗第4周与第8周的小鼠肿瘤细胞增殖率均低于模型组,超声2组低于超声1组,差异均有统计学意义(P<0.05)。超声1组与超声2组治疗第4周与第8周的CD4⁺T细胞比例和CD8⁺T细胞比例高于模型组,超声2组高于超声1组,差异均有统计学意义(P<0.05)。超声1组与超声2组治疗第4周与第8周的Bcl-2、MMP-9...     

双歧杆菌联合多磺酸黏多糖封包及地奈德乳膏治疗慢性湿疹的效果及对炎症因子和外周血T淋巴细胞亚群的影响

目的：探究双歧杆菌联合多磺酸黏多糖封包及地奈德乳膏治疗慢性湿疹的疗效及对炎症因子和外周血T淋巴细胞亚群的影响。方法：选取2020年6月至2021年12月海南省第五人民医院收治的110例慢性湿疹患者为研究对象,按随机数字表法随机分为对照组与观察组,每组55例。对照组予以多磺酸黏多糖封包+地奈德乳膏治疗,观察组在对照组基础上增加双歧杆菌治疗。比较两组临床疗效及治疗前后瘙痒、皮损面积、皮损严重程度评分及血清炎症因子[干扰素-γ(interferon-γ,IFN-γ)、白细胞介素-4(interleukin-4,IL-4)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)]、外周血T淋巴细胞亚群(CD4⁺、CD8⁺、CD4⁺/CD8⁺)水平。结果：观察组临床总有效率明显高于对照组(89.09%vs 70.91%,P<0.05)。观察组治疗后瘙痒、皮损面积、皮损严重程度评分均明显低于对照组(均P<0.05)。与对照组相比,观察组治疗后血清IFN-γ水平更高,血清IL-...     

Effect of blood donation on the establishment of normal ranges of lymphocyte subsets

BACKGROUND: Absolute counts of CD4+ T-lymphocytes are used in the management of patients with human immunodeficiency virus infection. Low absolute counts of CD3+CD4+ cells have also been observed in healthy people–a phenomenon called idiopathic CD4 lymphocytopenia. It is common practice for normal ranges for lymphocyte subsets to be derived from samples taken from blood donors. STUDY DESIGN AND METHODS: A sample of EDTA blood was taken through the donation line tubing, after donation from 565 blood donors in Sydney, Australia, who were selected from a range of age groups. An additional 12 donors provided a predonation sample as well as a postdonation sample. Hematologic assays were performed on two analyzers. Samples were stained for CD3, CD4, CD8, CD19, and CD56 and analyzed on a flow cytometer. RESULTS: Three donors were found to have absolute CD3+CD4+ counts < 300 cells per microL. The percentage of CD3+CD4+ cells was found to increase with age. Both the percentage and the absolute count of CD3+CD8+ cells decreased with age, which resulted in an increased CD4:CD8 ratio with age. Men had consistently higher absolute counts of CD3-CD56+ cells than women. The 12 additional donors all had greater percentages of CD3+CD4+ cells and lower absolute counts for CD3+, CD3+CD4+, CD3+CD8+, CD19+ and CD3-CD56+ cells after donation than they had before donation (p < 0.001). CONCLUSION: It is not satisfactory to base normal ranges for lymphocyte subsets on donor blood, from which the blood sample has been obtained after donation.     

健康成人外周血T淋巴细胞亚群绝对计数正常值调查

目的建立健康成年人外周血T淋巴细胞亚群绝对计数正常参考值.方法使用三标荧光抗体及TriCOUNT计数管,分别对298例健康成年人外周血T淋巴细胞亚群进行标记,采用FACSCaliburTM型流式细胞仪进行检测并分析结果,SPSS 10.0 软件进行统计学处理.结果 18～57岁健康成年人外周血中CD3 细胞绝对计数值为(1 242±404)个/μl ;CD3 CD4 细胞的绝对计数值为(651±209)个/μl ;CD3 CD8 细胞的绝对计数值为(524±283)个/μl ;CD3 CD4 CD8 细胞的绝对计数值为(17±10)个/μl .男性CD3 、CD3 CD8 细胞数比女性显著升高(P＜0.01).结论在相同地域、相同种族,可以建立健康成年人外周血的T淋巴细胞亚群绝对计数正常参考值.     

Circannual variation in lymphocyte subsets, revisited

BACKGROUND: Circadian and circannual variations in lymphocyte subsets, especially CD8+ T-lymphocytes, have been reported. This study focuses on CD4+ T-lymphocyte seasonal variation over a 6-year 8-month period. STUDY DESIGN AND METHODS: Lymphocyte subsets were quantitated monthly for four healthy individuals from 1986 through 1992 as part of a flow cytometry quality-control program. RESULTS: In general, there were no significant seasonal changes in the total number of white cells or in total lymphocyte counts. The absolute numbers of CD4+ T-lymphocytes were lowest in summer when the CD8+ T-lymphocytes were highest. Mean CD4+ T-lymphocyte counts were 846, 967, 618, and 695 per microL for Subjects 1 through 4, respectively, in winter and 432, 670, 355, and 766 per microL, respectively, in summer. Two healthy subjects had CD4+ T-lymphocyte counts lower than 300 per microL on one or more occasions during the study period. In three of the four subjects, the percentage of B-lymphocytes in winter was almost double that in summer. In one of the four subjects, no circannual rhythm was observed in these lymphocyte subpopulations. CONCLUSION: The seasonal variation in CD4+ T- lymphocyte counts demonstrated in three healthy individuals over almost 7 years is again of interest in light of renewed consideration of using surrogate tests, such as CD4+ T-lymphocyte counts, to screen for AIDS- like diseases that may be in the blood supply.     

Analysis of lymphocyte subsets in HIV-negative neurosyphilis patients

It is unclear how Treponema pallidum affects the immune response among various lymphocyte subsets in neurosyphilis patients with different clinical stages. In order to determine the immune response by T. pallidum infection, we detected the peripheral blood lymphocyte subsets among 14 asymptomatic neurosyphilis patients, 19 early neurosyphilis patients, 9 late neurosyphilis patients, and 50 healthy persons. The result indicated that the number of CD3+CD8+ lymphocytes was significantly higher in neurosyphilis patients than in the control group (χ² = 4.427, P = 0.035). The number of CD3+CD8+ lymphocytes was significantly higher in the asymptomatic neurosyphilis group than in the early neurosyphilis group, late neurosyphilis group, and control group (F = 4.644, P = 0.005). The number of NK cells was significantly lower in neurosyphilis patients than in the control group (χ² = 13.226, P = 0.000). The number of NK cells in neurosyphilis patients with different clinical stages was also lower than in the control group (F = 4.402, P = 0.006). The number of CD3+ lymphocytes, CD3+CD4+ lymphocytes, and B lymphocytes had no difference among the 4 groups. The results indicated that the progression of neurosyphilis may be related to the continued reduction in the number of NK cells and to the continued increase in CD3+CD8+ lymphocytes.     

常见恶性肿瘤患者机体淋巴细胞亚群和活化T淋巴细胞的分析

目的：对比研究常见恶性肿瘤患者术前与术中淋巴细胞亚群及活性T 淋巴细胞差异状况,研究手术对恶性肿瘤患者淋巴细胞亚群及其活化的影响。方法利用流式细胞仪测42例常见恶性肿瘤患者手术前、中的淋巴细胞亚群及其活性细胞。结果恶性肿瘤患者术中 CD3＋、CD4＋、CD8＋较术前有所降低,CD3＋与 CD4＋淋巴细胞差异有统计学意义（P ＜0．05）,与 CD8＋差异无统计学意义（P ＞0．05）。NK、CD19＋、CD3＋ HLA-DR＋明显升高,NK 细胞与 CD19＋差异无统计学意义（P ＞0．05）,与CD3＋ HLA-DR＋差异有统计学意义（P ＜0．05）。结论恶性肿瘤患者细胞免疫功能处于抑制状态,手术使恶性肿瘤患者的细胞免疫功能进一步损害。手术、创伤等的应激反应使 NK、CD19＋、活性 T 淋巴细胞的表达上升。恶性肿瘤患者建议手术前使用免疫反应调节剂增强患者的细胞免疫功能,以确保术后的放、化疗等后续治疗的效果。