艾灸对感染EHFV大鼠血桨血栓素和前列环素的调整作用

<正> 流行性出血热(EHF)的基本病理改变为广泛性小血管损害。血浆血栓素(TXA_2)和前列环素(PGI_2)是体内调节血小板-血管壁功能的重要因子。因此,这两种血管活性物质与EHF的发展和转归有其重要的内在联系。我院周楣声等应用中医灸法治疗     

Influence of moxibustion on TXA2 and PGI2 in plasma of rat infected epidemic hemorrhagic fever virus (EHFV)]

In this paper, the model of rat infected EHFV was made and the influence of moxibustion on TXA2 and PGI2 in its plasma was observed. The results show that the content of TXA2 increased and PGI2 decreased in rat significantly after the abdominal inoculation of EHFV, and the content of TXA2 decreased and PGI2 increased markedly in rat infected EHFV to normal level after treatment with moxibustion, suggesting that the regulative function of moxibustion on TXA2 and PGI2 is one of its nerve-endocrine-immune regulations to the body, and there is an important significance. This study provides an important reference for mechanism exploration of moxibustion preventing and treating EHF.     

Experiment research on moxibustion treating infected EHFV rat

In this experiment, we established a rat model of EHFV infected by abdominal cavity, and observed the effect of moxibustion on the changes of antigens, antibodies, neural transmitters in blood and tissue on 60 weanling Wistar rats with EHF. The rats were divided into three groups: normal control group (N. group), EHF control group (C. group) and EHF with moxibustion group (M. group) at random. Moxibustion was given at "Shenshu" for 30 minutes daily, 7 days. The 14th day after infection of EHFV, blood and lungs of the rats were fetched. The results obtained are as follows: 1. The effect value of EHF antibodies dilution: in C. group, it is 1:267 +/- 82 (n = 8), in M.group, it is 1:336 +/- 176 (n = 10), there is a significantly different of specific antibodies between C.group and M.group. The results show that moxibustion had an effect of enhancing the function of immune system in rats with EHF. 2. The examination rate of EHFV antigens in lungs, in C.group, it is 62.5% (n = 8), in M.group, it is 49.4% (n = 16). The results show that moxibustion may enhance the immune function and the effect of eliminating virus of lymphocytes in lungs. 3. The value of BUN in serum: in N.group, it is 15.3 +/- 2.50 (n = 10), in C.group, it is 24.63 +/- 7.24 (n = 10), and in M.group, it is 16.36 +/- 4.59 (n = 26). There is a significantly different between C.group and N.group or M.group (P less than 0.001). There is no significantly different between N.group and M.group (P greater than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)     

艾灸对感染EHF病毒大鼠的实验研究

<正> 流行性出血热(EHF)属于中医学温疫、疫疹、疫痧等范畴。我院应用祖国医学灸法在疫区治疗EHF病人能退热、抗体克、防治肾功能损害和缩短疗程,对出血热各期治疗均有较好的疗效。因此,深入探讨艾灸治疗EHF的机理研究具有重要的理论和临床意义。本实验应用腹腔感染EHF病毒的离乳大鼠为模型,观察灸治对大鼠病毒抗原、抗体及血液和组织内神经递质的影响。     

COMPARISON RESEARCH ON THE EFFECTS OF ELECTROACUPUNCTURE AND MOXIBUSTION ON THE BRAIN-GUT FUNCTIONS OF PATIENTS WITH DIARRHEA-PREDOMINANT IRRITABLE BOWEL SYNDROME

Objective: Electroacupuncture(EA) and moxibustion are to some extent effective for diarrhea-predominant irritable bowel syndrome(D-IBS); however, it is not clear whether their regulations on brain-bowel axis of D-IBS patients are different.The research is expected to provide a suitable clinical treatment by comparing the regulation of EA and moxibustion on digestive tract, psychological symptoms, and brain functional areas of D-IBS patients.Methods: A total of 62 cases of D-IBS patients were randomly divided into EA group(n=32) and moxibustion group(n=30).ST-36 and ST-37 were selected in the two groups.Acupuncture followed by electric stimulation was given to the EA group while warm moxibustion was given to the moxibustion group, once a day.Results: Both the EA group and the moxibustion group reported significant improvement in abdominal pain and abdominal distension(P0.001, P0.001).The moxibustion group reported better improvement in defecation frequency, defecation emergency and stool features than the EA group(P=0.001, P0.001, P0.001, P0.001).The moxibustion group reported better improvement in anxiety(HAMA) and depression(HAMD)(P0.001, P0.001).Both the EA and moxibustion groups can significantly reduce colonic mucosa 5-HT, 5-HT3R and 5-HT4R expression after treatment(P0.001, P0.001, P0.001), with moxibustion better than EA in 5-HT(P=0.024).The moxibustion group reported decreased activated voxel values of brain left IC, right IC and PFC regions after given stimulation of 150 mL CRD after treatment(P=0.011, P=0.023, P=0.009).The EA group reported decreased activated voxel values of PFC region(P=0.025).Conclusion: EA and moxibustion are different in the regulation of brain-bowel axis in D-IBS patients.Moxibustion can significantly improve frequent defecation, defecation emergency, watery stool and other digestive symptoms, anxiety, depression and other psychological symptoms as well as abnormal excitation in brainrelative functional IC and PFC regions of D-IBS patients.It suggests that moxibustion is a more effective therapy than EA in the treatment of D-IBS.     

EFFECT OF MOXIBUSTION OF DAZHUI(GV 14)ON CELLULAR IMMUNE FUNCTION IN TUMOR-BEARING MICE

Moxibustionisoneoftheimportantthera-peuticmethodsistraditionalChinesemedicine.Itswiderangingregulatoryeffectonorganismwasprovedbyalargenumberofpracticeandre-search.Withthemordernimmunologydevelop-ment,thestudiesoftheimmuneregulatoryef-fectofacupuncturemoxibustionhavebeenmorewidespreadanddeepgoinganditbecameimpor-tantwayofapproachingthemechanismintreat-ment.ofdiseasesbyacupuncture/moxibustion.Thevastamountofclinicalobservationshowedthatacupuncture/moxibustiontherapycouldal-leviatethesymptomofth…     

艾灸神阙穴对不同程度力竭运动大鼠海马区单胺类神经递质的影响

目的:探讨艾灸神阙穴对不同程度力竭运动大鼠海马区单胺类神经递质的影响。方法:将72只SD雄性大鼠按随机数字表法分为正常组8只、模型组和艾灸组各32只,根据造模或治疗时间将模型组和艾灸组随机分为1、4、7、10次亚组,每亚组8只,采用游泳实验建立力竭模型。艾灸各亚组大鼠造模后即刻温和灸神阙穴15min,隔日1次,各亚组大鼠完成相应力竭运动后24 h检测海马组织5-HT、DA、NE的含量。结果:与正常组比较,模型各亚组5-HT含量均明显升高,模型1、4、7次亚组NE含量均明显升高,模型1次亚组DA含量明显升高,模型10次亚组却明显降低。与相应模型亚组比较,艾灸4次亚组NE含量明显升高,艾灸7、10次亚组5-HT含量显著降低,DA、NE含量明显升高。结论:艾灸神阙穴可以通过调节反复力竭运动大鼠海马区神经递质,促进神经细胞的功能恢复,改善机体疲劳状态,为今后临床应用提供一定的实验基础。     

艾灸大椎穴对感染EHFV大鼠脾T、B淋巴细胞及抗体IgG的影响观察

材料和方法①实验动物及分组：离乳20日Wister大鼠33只，体重180～200g，安徽省实验动物中心提供。实验动物分为3组：感染艾灸组：每只大鼠以1000LD50病毒量腹腔注射0．5mlE－HFV－A96株，并于大椎穴处施灸。感染对照组：接种病毒，不施灸。正常对照组：不接种，不施灸。②艾灸方法：于接种EHFV第二天对感染大鼠按实验动物取穴标准取“大推”穴，将艾绒捏成麦粒大，直接放在穴位处，点燃施灸，每次三壮，每日1次，连续7天。③检测指标及方法：动物种毒后第14天处死取血，并即时取脾测定T、B淋巴细胞量，测EHF抗体IgG（免疫荧光法）…     

乙酰胆碱与电针对脑内5羟色胺代谢的影响

<正> 为了研究针刺镇痛过程中 ACh 和单胺类递质之间的关系,本文观察了电针镇痛过程中 ACh、5-HT、5-HIAA,含量的变化,又利用胆碱酯酶抑制剂(毒扁豆碱)和 ACh合成阻断剂(密胆碱)分别升高或降低脑内ACh 的方法观察电针对5-HT 代谢的影响。发现:针刺镇痛过程中5-HT 的代谢变化与ACh 有一定的关系。     

Clinical Study on Acupuncture in Treating Diarrhea-predominant Irritable Bowel Syndrome

目的：观察腹泻型肠易激综合征（irritable bowel syndrome，IBS）患者结肠粘膜5．HT变化及隔药灸治疗的临床疗效，初步探讨其作用机理。方法：-临床收集腹泻型IBS患者73例，随机分为隔药灸组（37例）和针刺组（36例）进行治疗，观察临床疗效，并观察隔药灸治疗对典型患者结肠粘膜5-HT表达的影响。结果：隔药灸组痊愈率为43．2％，针刺组痊愈率为36．1％，两组间无统计学差异（P〉0．05）；隔药灸治疗能够改善患者结肠粘膜5-HT异常增高的表达。结论：隔药灸能够改善腹泻型IBSIIg床症状，可能是通过调节5-HT表达发挥治疗作用。     

艾灸治疗类风湿性关节炎抗炎免疫作用机理的研究

为了探讨艾灸治疗RA抗炎免疫作用的机理 ,我们建立免疫性炎症———大鼠佐剂性关节炎模型等 ,从神经 内分泌 免疫调控入手 ,在整体、器官、细胞水平开展灸治RA的实验研究。结果艾灸“肾俞”、“足三里”穴具有抗炎免疫作用 ,其机理与灸疗抑制TNF、IL 1等炎症因子的释放 ,增强与改善机体的免疫功能 ,保护胸腺、脾脏等免疫器官 ,纠正炎症时自由基代谢的紊乱 ,调整NO、NE、5 HT神经递质的失衡 ,促进内环境稳定等多方面因素有关。实验证实 ,外周交感神经参与艾灸对免疫的调节 ;灸疗的部分作用是通过肾上腺皮质系统发挥的 ;海马可能是灸疗信息中枢整合的重要环节 ;HPA轴是灸疗作用中一条重要的神经体液性途径 ;松果体 (MT)可能是艾灸抗炎免疫的一个高位调节点。提示灸疗抗炎免疫作用是其治疗RA的主要机制之一     

艾灸对吗啡依赖小鼠细胞免疫功能的影响

本文观察了艾灸“关元”穴对Ｍｏｒ依赖小鼠细胞免疫的影响。结果表明,Ｍｏｒ可抑制体重增加,降低细胞免疫功能,与正常组相比差异显著。艾灸“关元”穴可促进Ｍｏｒ依赖小鼠淋巴细胞转化,增强 ＮＫ细胞的活性,与对照组相比 Ｐ＜０．００１。提示艾灸具有增强 Ｍｏｒ依赖小鼠细胞免疫功能的作用,为灸疗脱毒促进康复的临床应用提供了实验依据。     

调肝、补肾、健脾方药对慢性心理应激大鼠单胺类神经递质影响的比较研究

目的：观察慢性心理应激反应大鼠血浆如下丘脑多巴胺（DA）、5－羟色胺（5－HT）、肾上腺素（E）、去甲肾上腺素（NE）的变化及调肝、补肾、健脾方药对其影响。方法：Wistar大鼠按体重随机分为6组，即对照组（C），模型组（M），调肝中药（加味四逆散）组（C1），人参总皂甙组9C2），补肾中药（肾气丸）组（C3），健脾中药（四君子汤）组（C4）。采用束缚限制活动空间的方法造成慢性心理应激大鼠模型，所有药物灌胃给药。采用电化学高效液相色谱分析方法检测血浆和下丘脑DA、5－HT、E、NE含量。结果：应激大鼠血浆和下丘脑DA、5－HT含量明显降低（均P＜0.01)，血浆NE、E含量明显升高（均P＜0.01)，而下丘脑NE含量无明显变化。血浆5－HT／NE、5－HT／E及下丘脑5－HT／NE均明显降低（均P＜0.01)。加味四逆散可以升高应激大鼠下丘脑和血浆中DA、5－HT含量（均P＜0.01)，并能降低血浆中NE、E含量（均P＜0.01)，升高下丘脑和血浆5－HT/NE以及血浆5－HT／E（P＜0.05或P＜0.01)。肾气丸、四君子汤及人参部 甙虽然对有些指标有影响，但对心理应激的整体调节作用不如加味四逆散。结论：加味四逆散对慢性心理应激大鼠血浆和下丘脑单胺类神经递质的代谢紊乱均有良好的调节作用，调肝、补肾、健脾治法方药中以调肝治法方药的综合调治作用最为显著。     

疏肝健脾方对腹泻型肠易澉综合征大鼠血浆及结肠组织5-HT、SP、VIP的影响

[目的]观察疏肝健脾方对腹泻型肠易激综合征大鼠血浆及结肠组织5-羟色胺（5-HT）、P物质（SP）和血管活性肠肽（VIP）的影响。[方法]将60只SD大鼠按随机数字表法随机分为正常组、模型组、得舒特组、疏肝健脾方低、中、高剂量组,使用乙酸灌肠加束缚的方法建立腹泻型肠易激综合征（D—IBS）大鼠模型,应用免疫组化法检测5-HT、SP及VIP。[结果]疏肝健脾方可以明显降低大鼠的粪便含水量（P〈0．05）;疏肝健脾方中、高剂量组腹部撤退反射（AWR）评分在不同扩张容量下均较模型组明显降低（P〈0．05）;疏肝健脾方可以降低大鼠血浆中5-HT和结肠组织中sP的含量,而血浆中SP、VIP及结肠组织中VIP含量无显著变化。[结论]疏肝健脾方可以改善大鼠的腹泻及内脏的高敏感性,并对5-HT及SP有调节作用,提示疏肝健脾方可以有效治疗D—IBS,并与调节胃肠激素有关。     

针刺与艾灸对慢性不可预见性温和应激抑郁大鼠前额皮质5-羟色胺系统的影响

目的:探讨针刺与艾灸对慢性不可预见性温和应激(CUMS)抑郁大鼠的疗效差异及其机制。方法:SD大鼠随机分为空白组、模型组、氟西汀组、针刺组、艾灸组,每组8只。采用CUMS复制抑郁大鼠模型。针刺组针刺"百会""大椎",艾灸组艾灸"百会""大椎",均每次20min,每日1次,共治疗3周。计算糖水消耗百分比,采用放射免疫法检测前额皮质5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)、色氨酸(Trp)含量,放射性配体受体结合分析技术计算前额皮质高低亲和力5-HT1A受体的结合常数(K)、结合容量(R)。结果:模型组糖水消耗百分比较正常组显著下降(P0.01)。治疗3周后,各治疗组糖水消耗百分比较之模型组显著升高(P0.01)。模型组5-HT含量低于正常组(P0.01),氟西汀组较模型组升高(P0.05),艾灸组显著低于氟西汀组(P0.01)。模型组5-HIAA含量与正常组比较差异无统计学意义(P0.05),各治疗组均显著低于模型组(P0.01),艾灸组显著低于针刺组(P0.01)。5-HT/5-HIAA比值,模型组显著低于正常组(P0.01),各治疗组均显著高于模型组(P0.01,P0.05),艾灸组低于氟西汀组(P0.05)。模型组Trp含量显著高于正常组(P0.01),各治疗组则均显著低于模型组(P0.01)。与正常组比较,模型组K 1、R 2显著下降(P0.05),与模型组比较,各治疗组高低亲和力5-HT1A受体的结合常数与容量均有上升趋势(P0.05)。结论:针刺与艾灸对CUMS抑郁模型大鼠的疗效相当,调节5-HT系统及5-HT1A受体功能可能是针刺与艾灸抗抑郁的作用途径之一。     

新冠肺炎疫情下艾灸防治帕金森病的临床应用分析＊

帕金森病（Parkinson''s disease，PD）是仅次于阿尔茨海默病的世界第二大神经系统退行性疾病，随着老龄化社会的到来，PD患病率呈明显上升趋势。全球爆发的新型冠状病毒肺炎（简称新冠肺炎）是一种急性传染性疾病，严重威胁人们的身体健康和生命安全。PD患者大多为老年人且合并有基础疾病，体质普遍较弱，是新冠肺炎的易感人群，一旦染病则发展为重症的风险很高。中医艾灸疗法在治疗PD及防治疫病方面发挥了很好的辅助治疗效果。通督扶阳灸法联合强壮穴艾灸治疗，既可以帮助PD患者改善临床症状，延缓病情进展，又可以提高机体免疫功能，增强抗病能力而防治新冠肺炎。     

EFFECT OF HEALTH-PRESERVING MOXIBUSTION ON PLASMA TESTOSTERONE AND ESTRADIOL OF THE OLD PEOPLE

Withthecomingofageingofthehumanbodythesexualglandwillbedegenerated.Ase-riesofcomplicatedregulationsandcontro1existinthehypothh1amus-hypophysis-sexualglandsystem.Intheageingprocessofthehumanbody,functionalchangesofthisneuroendocrinesystemgiverisetomoreandmoreattentiona-mongpeople.Whenmenandwomenentertheprodromalstageofsenility,boththeplasmatestosteroneandestradiollevelschangeconsider-ablyalongwithincreaseofage.Testosteroneofmenpresentsatendencyofdecreaseandestradiolofwomenalsodeclines,indicatin…     

灸百会 膻中和松弛疗法对抑郁症患者血浆五羟色胺的影响

目的:观察灸百会、膻中和松弛疗法对抑郁症患者血浆五羟色胺(5-HT)含量的影响。方法:将60例患者,按就诊先后随机分为治疗组(灸百会、膻中和松弛疗法组)、对照组(西药氟西汀组),各组8周为1个疗程;各组治疗前后分别测定汉密尔顿抑郁量表(HAMD)分值及血浆中5-HT的含量,用高效液相色谱法及电化学分析法检测治疗前后患者血浆5-HT的含量,通过对比治疗前后的HAMD分值及血浆5-HT含量的变化,统计临床数据。结果:灸法和松弛疗法可明显改善抑郁症患者HAMD的评分,其改善情况与单纯的西药组相当;生化指标5-HT含量治疗前、后用x-±s表示,组内均数比较有显著性差异,P(0.05。结论:灸百会、膻中和松弛疗法可提高抑郁症患者体内5-HT浓度,与西药氟西汀的疗效相当。     

电针、艾灸、埋线对痛经大鼠T细胞亚群水平的影响

目的观察电针、艾灸、埋线对实验性痛经大鼠T细胞亚群水平的影响。方法已烯雌酚和催产素模拟制作痛经模型,观察痛经大鼠的扭体反应情况和大鼠胸腺、脾脏的变化,检测大鼠血浆CD3、CD4、CD8的水平。结果电针、艾灸、埋线能明显减少痛经模型30 min内扭体次数,与模型组比较差异有统计学意义(P<0.01);模型组CD3、CD4与对照组比较差异有统计学意义(P<0.01),存在明显的免疫功能低下。电针、艾灸、埋线组CD3、CD4与模型组比较差异有统计学意义(P<0.01)。模型组免疫器官胸腺和脾脏发生了明显的病理变化,电针、艾灸、埋线组的胸腺和脾脏的病理变化较模型组有一定的改善。结论电针、艾灸、埋线不但有很好的止痛或缓解疼痛的作用,而且对拟原发性痛经大鼠外周血T淋巴细胞亚群和免疫器官都有影响,在一定程度上拮抗了原发性痛经出现的免疫功能低下。     

芳香疗法配合灸法治疗抑郁症临床研究

目的：观察芳香疗法和灸法治疗抑郁症的临床疗效及对患者血浆5-HT含量的影响。方法：60例按就诊先后随机分为两组。治疗组用芳香疗法和灸法、对照组用西药氟西汀治疗,8周为一疗程;治疗前后分别测定汉密尔顿抑郁量表（HAMD）分值及血浆中5-HT的含量,用高效液相色谱法及电化学分析法检测治疗前后患者血浆5-HT的含量,通过对比治疗前后的HAMD分值及血浆5-HT含量的变化,统计临床数据。结果：芳香疗法和灸法可明显改善抑郁症患者HAMD的评分,其临床疗效与单用西药氟西汀相当。生化指标5-羟色胺含量治疗前、后有显著性差异（P〈0．05）,组闻比较无显著性差异（P〈0．05）。结论：芳香疗法和灸法可提高抑郁症患者体内5-HT浓度,其临床疗效与西药氟西汀组的疗效相当。