花叶假杜鹃总黄酮含量测定

目的：建立花叶假杜鹃中总黄酮含量的测定方法,并测定其含量。方法：采用超声提取法,以80%乙醇为提取溶剂提取药材中黄酮类成分,以紫外-可见分光光度法于510 nm处测定其吸光光度,建立标准曲线,计算总黄酮含量。结果：以芦丁为对照品,总黄酮在0.02502~0.08340 mg.mL-1浓度范围内符合Beer定律,回归方程为A=10.339 C＋0.0115（r=0.9996）;平均加样回收率为96.2%（RSD=2.65%）;得花叶假杜鹃每克生药中总黄酮平均含量为47.797 mg。结论：利用紫外分光光度法测定花叶假杜鹃中总黄酮的含量,简便快速且有良好的稳定性、准确性和重现性,可用于药材的质量控制。     

柴胡口服液中总黄酮含量测定

目的测定柴胡口服液中总黄酮的含量。方法采用分光光度法,以芦丁为对照品测定柴胡口服液中总黄酮的含量。结果在4~32μg.m l-1的范围内,样品浓度与吸光度有良好的线性关系,线性回归方程为A=0.011 55C+0.005 39(r=0.999 72),加标回收率为102.0%(n=5,RSD=1.35%),柴胡口服液总黄酮平均含量为0.339 mg.m l-1。结论本方法简便、快速、准确、灵敏,可作为柴胡口服液中总黄酮的含量测定的方法。     

圆盖阴石蕨中总黄酮的超声波提取及含量测定

目的:运用超声波提取圆盖阴石蕨中总黄酮并对总黄酮的含量进行测定.方法:正交设计优选圆盖阴石蕨中总黄酮的提取条件;以芦丁为标准品,利用黄酮类分子中的酚羟基可与Al3 在碱性溶液中显色原理,在500 nm处用分光光度法测定总黄酮的含量.结果:用70%乙醇在50 ℃下超声波提取时间为0.5 h,提取体积80 ml,提取次数为1次.圆盖阴石蕨根茎中总黄酮的含量为1.65%,平均回收率为98.30%,RSD=0.68%(n=5),圆盖阴石蕨叶子中总黄酮的含量为3.42%,平均回收率为98.53%,RSD=0.70%(n=5).结论:运用超声波提取圆盖阴石蕨中总黄酮,提取时间短,收率高.     

紫外分光光度法测定核桃花总黄酮含量

目的 :采用紫外分光光度法测定核桃花中的总黄酮含量.方法 :以芦丁为对照品,采用超声波提取和AlCl3显色,在417nm处测定吸光度,计算核桃花的总黄酮含量.结果 :芦丁的线性范围为2.29~29.96μg·mL-1,回归方程A=0.0262C+0.0421(r=0.9996,n=6);平均回收率为100.2%,RSD=0.91%;核桃花中总黄酮含量为10.18mg·g-1.结论 :该文首次以AlCl3显色分光光度法测定核桃花总黄酮含量,所用方法重复性好,结果准确.     

微波提取车前穗中总黄酮及其含量测定

目的：从车前穗中提取总黄酮,并测定其含量。方法：运用微波技术提取车前穗总黄酮,用比色法测定总黄酮含量。结果：测得车前穗中总黄酮含量为5．21％,平均回收率为97．9％,RSD=1．74％(n=6)。结论：首次运用微波技术从车前穗中提取出总黄酮,本方法反应速度加快,实验结果令人满意。     

枳椇子中总黄酮的含量测定

目的:测定江西枳椇子中总黄酮的含量.方法:以槲皮素为对照品.用紫外分光光度法于256nm处测定江西枳椇子中总黄酮的含量.结果:江西枳椇子中总黄酮的质量分数为1.96%,RSD=0.85%(n=5);回归方程y=75.3x-0.021,槲皮素在2～10ug·mL-1(r=0.9998)范围内线性关系良好,平均回收率为100.6%.结论:方法简便易行,准确可靠,可作为枳椇子总黄酮质量控制的方法,为开发利用枳椇子提供了科学依据.     

女贞属苦丁茶中总黄酮的含量测定

目的测定贵州不同产地苦丁茶总黄酮化合物的含量。方法 L9（34）正交试验优选提取条件,用比色法测定苦丁茶中总黄酮化合物含量。结果最佳提取条件为：用60倍90%乙醇提取苦丁茶粗粉2次,每次1h,用比色法测定总黄酮化合物的含量。芦丁标准品在0.4868-2.434mg浓度范围线性关系良好,线性方程为：y=0.3139x-0.0062,r=0.9960。平均回收率为98.77%,RSD为1.5%。贵州多个产地的苦丁茶中总黄酮的含量均在30%以上。结论贵州不同产地的苦丁茶中总黄酮的含量较高且存在差异,本方法方便,可靠,重复性好,可用于苦丁茶中总黄酮的含量测定。     

分光光度法测定绞股蓝中总黄酮的含量

目的建立用分光光度法测定绞股蓝中总黄酮含量的方法。方法采用分光光度法,以芦丁为对照品,在波长510nm处对样品中的总黄酮进行含量测定。结果总黄酮在0.2~1.0mg.ml-1范围内呈良好的线性关系(r=0.9992);本方法的平均加样回收率为100.4%,RSD为1.68%(n=6)。结论本方法稳定、简便、快速,适用于绞股蓝中总黄酮的含量的测定。     

南瓜叶中总黄酮含量测定

目的测定南瓜叶中总黄酮的含量。方法以芦丁为对照品,采用分光光度法在510 nm处测定南瓜叶中总黄酮含量。结果芦丁对照品溶液浓度8~40 mg.L-1时与吸光度有良好的线性关系,线性回归方程为A=0.011 8C-0.025 1(r=0.999 2),加样回收率为100.9%(n=5),相对标准偏差为1.35%,南瓜叶总黄酮平均含量为2.65 mg.g-1。结论本方法简便、快速、准确、灵敏,可作为南瓜叶中总黄酮的含量测定方法。     

麻黄中总黄酮和多糖的微波提取与含量测定

目的:从麻黄中提取总黄酮和多糖并测定其含量.方法:运用微波技术提取麻黄总黄酮和多糖,用比色法测定总黄酮和多糖含量.结果:麻黄中总黄酮含量为6.64%,平均回收率为100.6%,RSD为1.34%(n=5);多糖含量为4.83%,平均回收率为99.58%,RSD为1.02%(n=5).结论:运用微波技术从麻黄中联合提取总黄酮和多糖,反应速度快,提取效率高.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.