复方四参饮对病毒性心肌炎患者细胞免疫功能的影响

为了研究复方四参饮对病毒性心肌炎患者细胞免疫功能的影响，测定了６０例患者的自然杀知性和２０例患者的淋巴细胞亚群。结果除了ＯＫＴ８外者显著低于正常值。经复方四参饮治疗后，除ＯＫＴ８外皆有显著升高。中医辨证分型表明，ＮＫ细胞功能下降以气阴两虚阻为明显，而Ｔ细胞亚群的改变以气虚组为显著。ＮＫ细胞活性与ＯＫＴ４／ＯＫＴ８值呈负相关。此外，早期ＮＫ细胞功能状况与疾病预后关系密切。     

辨证分型论治葡萄膜炎及其对免疫功能的影响

目的 观察中药治疗葡萄膜炎的效果及其对免疫功能的影响。方法 葡萄膜炎患者４４例按辨证分为热毒炽盛和肝肾阴虚两型,分别使用中药消炎一号方和消炎二号方治疗,并分别于治疗前后检测Ｔ细胞亚群、免疫球蛋白、补体和ＩＬ－２的变化。结果 热毒炽盛型患者ＯＫＴ４、ＯＫＴ４／ＯＫＴ８、ＩＬ－２和ＩｇＭ治疗后比治疗前下降（Ｐ〈０．０５或０．０１）。肝肾阴虚型患者治疗后ＯＫＴ８、ＩＬ－２和补体Ｃ３升高（Ｐ〈０．０５）：     

清热止血药对乳糜尿患者免疫功能的观察

为了探讨乳糜尿治疗机理，本研究采用清热止血药为基本方，治疗乳糜尿患者３０例，并进行了治疗前后免疫功能测定。结果表明，治前患者外周血ＯＫＴ３、ＯＫＴ４水平均低下，ＯＫＴ８升高，ＯＫＴ４／ＯＫＴ８倒置；治疗后ＯＫＴ３、ＯＫＴ４均增高，ＯＫＴ８下降，倒置的ＯＫＴ４／ＯＫＴ８被纠正。治疗前体液免疫水平也普遍低下，治后均有回升。     

参麦注射液对晚期癌症患者sIL－2R、LAK及NK细胞活性影响

本研究测定了６０例晚期癌症患者用参麦注射液治疗前后血清可溶性白细胞介素－２受体、淋巴因子活化的杀伤细胞和天然杀伤细胞的活性，并以４０例正常人为对照。结果显示：治疗前癌症患者ｓＩＬ－２Ｒ显著升高，ＬＡＫ细胞与ＮＫ细胞活性显著减低。而上述变化在胃癌、肠癌与肺癌之间无显著差异。治疗后３种癌症患者的ｓＩＬ－２Ｒ均有显著下降，ＬＡＫ与ＮＫ细胞活性均有显著增强。而ｓＩＬ－２Ｒ与ＬＫＡ及ＮＫ细胞活性之间无相关性     

参麦注射液对晚期癌症患者sIL－2R、LAK及NK细胞活性影响

本研究测定了６０例晚期癌症患者用参麦注射液治疗前后血清可溶性白细胞介素－２受体（ｓＩＬ－２Ｒ）、淋巴因子活化的杀伤细胞（ＬＡＫ）和天然杀伤细胞（ＮＫ）的活性，并以４０例正常人为对照。结果显示：治疗前癌症患者ｓＩＬ－２Ｒ显著升高（Ｐ＜０．０５），ＬＡＫ细胞与ＮＫ细胞活性显著减低（Ｐ＜０．０５）。而上述变化在胃癌、肠癌与肺癌之间无显著差异（Ｐ＞０．０５）。治疗后３种癌症患者的ｓＩＬ－２Ｒ均有显著下降（Ｐ＜０．０５），ＬＡＫ与ＮＫ细胞活性均有显著增强（Ｐ＜０．０５）。而ｓＩＬ－２Ｒ与ＬＫＡ及ＮＫ细胞活性之间无相关性。提示晚期癌症患者免疫功能低下，参麦注射液对癌症患者的免疫功能有广泛的调节作用。     

益气活血、化瘀通腑法治疗子宫内膜异位症的临床观察

目的：探讨益气活血，化瘀腑法治疗子宫内膜异位症的作用机理。方法：４５例子宫内膜异位症患者随机分为中药内异１＾＋治疗组和三苯氧胺治疗组，观察临床症状的改善情况，外周血ＮＫ细胞活性，Ｔ细胞亚群，白细胞介素２，抗子宫内膜抗体等变化情况。结果：内异症患者体内存在广泛的免疫紊乱，经过本法治疗后，患者外周血ＮＫ细胞活性升高，ＣＤ４降低，ＣＤ８升高，ＣＤ４／ＣＤ８比值下降，ＩＬ－２降低，５２．８８％的患者ＥｍＡ     

益气阴活血祛浊方对30例慢性肾功能衰竭气阴两虚证患者细胞免疫 …

为了探讨慢性肾功能衰竭（ＣＲＦ）气阴两虚患者的细胞免疫状态及中药治疗的影响,对３０例ＣＲＦ气阴两虚证患者进行了Ｔ淋巴细胞亚群、自然杀伤（ＮＫ）细胞,可溶性ＩＬ－２受体（ｓＩＬ－２Ｒ）检测,结果显示,ＣＲＦ气阴两虚证患者ＣＤｓ、ＣＤ４、ＣＤ４／ＣＤｓ比值、（ＮＫ）细胞水平均明显低于正常对照组（Ｐ均〈０．０１）;ＣＤｓ水平明显高于正常对照组（Ｐ〈０．０５）;ｓＩＬ－２Ｒ水平明显高于正常对照组（Ｐ〈０．     

益心灵对病毒性心肌炎患者NK细胞活性及T细胞亚群的影响

观察５２例病毒性心肌炎（ＶＭＣ）患者及２４例正常对照者（ＮＣ）外周血自然杀伤细胞（ＮＫＣ）活性及Ｔ淋巴细胞亚百分率与Ｔ辅助细胞／抑制Ｔ细胞（ＴＨ／Ｔｓ）比值差异。比较常规疗法及其伍用益心灵口服液对ＶＭＣ患乾ＮＫＣ活性及Ｔ淋巴细胞亚群的影响。结果表明，ＶＭＣ患乾ＮＫＣ活性在急性ＶＭＣ或慢性ＶＭＣ后遗症症者均显著低于ＮＣ组（Ｐ〈０．００１）；Ｔ淋巴细胞亚群急性ＭＶＣ患者总Ｔ细胞（ＣＤ３）、Ｔ辅助细胞（     

慢性肾病与尿SIL-2R及T淋巴细胞亚群关系

为了探讨中医虚证微观辨证指标，测定了气虚型及气阴两虚型“慢肾病”８４例的尿ＳＩＬ－２Ｒ及Ｔ淋巴细胞亚群。结果示：２型与对照组比均有尿ＳＩＬ－２Ｒ增高、ＯＫＴ３、ＯＫＴ４降低（Ｐ＜０．０１），但气阴两虚型ＯＫＴ４／ＯＫＴ８比例降低（Ｐ＜０．０１）。结论为尿ＳＩＬ－２Ｒ及Ｔ淋巴细胞亚群改变可作为这２型慢肾病的微观辨证指标。     

复方参芪对难治性肾病综合征T淋巴细胞亚群,白介素2的影响及疗效观察

对７４例难治性肾病综合征随机分两组，治疗组以复方参芪片联合强的松、环磷酰胺及肝素治疗，对照组单用西医治疗。结果治疗组治后ＯＫＴ４、ＯＫＴ４／ＯＫＴ８比例及ＩＬ－２均上升，疗效亦高于对照组。认为复方参芪片具有调整细胞免疫的作用，从而提高疗效。     

EFFECT OF MOXIBUSTION OF DAZHUI(GV 14)ON CELLULAR IMMUNE FUNCTION IN TUMOR-BEARING MICE

Moxibustionisoneoftheimportantthera-peuticmethodsistraditionalChinesemedicine.Itswiderangingregulatoryeffectonorganismwasprovedbyalargenumberofpracticeandre-search.Withthemordernimmunologydevelop-ment,thestudiesoftheimmuneregulatoryef-fectofacupuncturemoxibustionhavebeenmorewidespreadanddeepgoinganditbecameimpor-tantwayofapproachingthemechanismintreat-ment.ofdiseasesbyacupuncture/moxibustion.Thevastamountofclinicalobservationshowedthatacupuncture/moxibustiontherapycouldal-leviatethesymptomofth…     

隔药饼灸对乏氧肿瘤细胞及肿瘤放疗免疫系统的影响

采用隔药饼灸结合1 37CSγ射线放疗对实体瘤 (HAC)小鼠进行研究 ,不同时间肿瘤体积及瘤重抑制率表明 ,艾灸能增强对乏氧肿瘤细胞的杀伤作用。小鼠荷瘤后NK、LAK、IL 6活性及IL 2含量均明显降低 ,放疗后除NK活性外 ,均进一步降低 ,经艾灸治疗可以明显提高NK、LAK及IL 6活性和IL 2含量 ,其中LAK活性达到正常水平。提示隔药饼灸可促进肿瘤放疗后免疫系统损伤的恢复     

Effects of moxibustion to zusanli (ST36) on alteration of natural killer cell activity in rats

Moxibustion is one of the major healing techniques in Oriental medicine. It has been widely used in many diseases such as rheumatoid arthritis, Hashimoto disease, breech presentation, etc. However, till now, effects of moxibustion on natural killer (NK) cell activity and relations between sympathetic nerve system (SNS) and the immune alteration induced by moxibustion were not well studied. This study was designed to evaluate effects of moxibustion on NK cell activity and the intervention of SNS in the alteration of NK cell activity induced by moxibustion. Splenic NK cell cytotoxicity was measured in a standard 4-hour 51Cr release assay. We measured the NK cell cytotoxicity after moxibustion stimulation for 1, 3, 5 and 7 days, and also measured the NK cell cytotoxicity after 3 and 7 days burn stimulation with similar temperature. Interleukin (IL)-2, -4 and interferon (INF)-gamma in serum were measured by rat IL-2, -4 and INF-gamma ELISA test kit. To evaluate the effects of sympathectomy on alteration of NK cell cytotoxicity, 6-hydroxydopamine (6-OHDA: 50 mg/kg) was used. We showed that NK cell activity of moxibustion stimulation group increased at the 3rd day, and declined at the 7th day in comparison with that of the control group. In the moxibustion stimulation group, NK cell activity was significantly higher than the sham group at the 3rd day. On the contrary, in the burn stimulation group, NK cell activity was significantly higher than that of the sham groups at 3rd and 7th days. INF-gamma level after 3 days in the moxibustion stimulation group was significantly higher than that of the sham group. IL-2 level among groups were not different. IL-4 was not detected in serum with this method. Sympathectomy abolished the NK cell activity alteration induced by moxibustion. The results suggest that moxibustion modulates NK cell activity, along with INF-gamma, and SNS is mediating these effects.     

艾灸对荷瘤小鼠免疫功能的影响

目的 :观察艾灸对荷瘤小鼠免疫功能的影响。方法 :将 40只荷瘤小鼠随机分为四组 ,对照组、艾灸组、5 氟脲嘧啶 ( 5FU)组、5FU +艾灸组。艾灸组、5FU +艾灸组分别在“中脘”、“神阙”、“关元”穴隔药饼灸 ,隔天 1次 ,治疗 8次。 5FU组、5FU +艾灸组在腹部注射 0 2 5mg/ 0 1mL5FU ,3天 1次 ,共治疗 4次。所有治疗完毕后 2 4hr处死动物。测定小鼠T淋巴细胞、自然杀伤细胞 (NK)及进行淋巴因子激活的杀伤细胞 (LAK)杀伤功能检测。结果 :艾灸组CD3、CD4、CD4/CD8比率、NK细胞、LAK杀伤活性比 5FU组显著提高 (P <0 0 5、P <0 0 0 5、P <0 1、P <0 1、P <0 0 1 ) ,差异明显 ,具有显著性意义。结论 :艾灸能提高小鼠的免疫力 ,而 5FU有抑制作用 ,使荷瘤小鼠免疫力降低     

Integrated traditional and Western medicine treatment of severe viral myocarditis]

33 patients divided into 2 groups for treatment. Group A (18 cases) early use of adrenal cortico-steroid treatment in combination with traditional Chinese medicine; group B (15 cases): early use of adrenal cortico-steroid treatment is combination with interferon, then compared the therapeutic effects of these 2 groups. The results showed that, in these 2 groups, the sinus-atrial block, atrial ventricular block, ventricular tachycardia, acute pump failure as well as the EKG changes were relieved and improved to the same degree. In group A, after therapy, the parameters of OKT3 (%) was elevated from 51 +/- 15.22 to 55.23 +/- 8.53; OKT4(%) was elevated from 30.06 +/- 11.47 to 47.32 +/- 10.87; ratio of OKT4/OKT8 was elevated from 1.11 to 1.53; all of their P values were < 0.01. NK cell activity was elevated from 8.36 +/- 3.75 to 13.08 +/- 5.77, P value < 0.05. In group B, the parameters of OKT3 (%) was elevated from 50.91 +/- 8.12 to 56.75 +/- 8.29; OKT4 (%) was elevated from 32. 55 +/- 6.78 to 45.13 +/- 10.85; ratio of OKT4/OKT8 was elevated from 1.00 +/- 0.21 to 1.37 +/- 0.16; P value < 0.05, < 0.05 and < 0.01 respectively. NK cell activity (%) was elevated from 8.20 +/- 3.08 to 14. 0.3 +/- 4.89, P value also < 0.01. Hence, not only in group A but also in group B, after therapy, the parameters of OKT3,4,8, OKT4/OKT8 and NK cell activity (%) were all improved. But there was no significant statistical difference between group A and B.(ABSTRACT TRUNCATED AT 250 WORDS)     

参麦注射液对胃癌化疗后免疫功能影响的观察

６３例胃癌患者随机分组观察,结果提示,化疗结合参麦注射液组（简称参麦组）Ｔ淋巴细胞计数（ＡＮＡＥ）上升（Ｐ＜０．０５）,化疗组（简称对照组）ＡＮＡＥ下降（Ｐ＜０．０５）;化疗后两组ＯＫＴ１值、ＯＫＴ４／ＯＫＴ８值均有下降,动态结果提示参麦组上述指标提前回升,第４周明显高于化疗前水平（Ｐ＜０．０５）,对照组第４周仍处于较低水平;化疗后参麦组血清可溶性白细胞介素－２受体（ｓＩＬ－２Ｒ）水平下降（Ｐ＜０．０５）,ＮＫ、ＬＡＫ水平上升（Ｐ＜０．０５）;对照组ｓＩＬ－２Ｒ水平无明显变化（Ｐ＞０．０５）,ＮＫ、ＬＡＫ水平下降（Ｐ＜０．０５）;两组化疗前后ＩｇＡ、ＩｇＭ、ＩｇＧ水平无明显变化（ｐ＞０．０５）。表明参麦注射液可改善患者免疫功能,有助于化疗的施行。     

艾灸治疗桥本氏甲状腺炎的免疫学观察

采用艾灸疗法治疗36例桥本氏甲状腺炎患者,观察患者治疗后T淋巴细胞亚群的变化及其对B细胞产生抗甲状腺自身抗体的影响。结果表明,经艾灸治疗后,患者外周血淋巴细胞OKT_4~+/OKT_8~+细胞比值和淋巴细胞分泌甲状腺抗体的水平都明显下降,而且两者的变化具有显著的相关性。作者认为,艾灸可能是通过调整T淋巴细胞亚群的比例关系来抑制B淋巴细胞产生抗甲状腺抗体的水平,从而治疗桥本氏甲状腺炎的。     

隔姜灸神阙治疗支气管哮喘疗效与T细胞亚群含量变化关系的分析

采用隔姜炎神阙治疗支气管哮喘２７例，治疗前检测外周血Ｔ细胞亚群Ｔ３、Ｔ４、Ｔ４／Ｔ８值及杀伤细胞（ＮＫ）含量均低于正常值。灸治后复检Ｔ３、Ｔ４、Ｔ４／Ｔ８值及ＮＫ细胞，含量均有不同程度升高。同时观察到临床症状、体征均有不同程度改善，说明艾灸治疗支气管哮喘的疗效与Ｔ３、Ｔ４、Ｔ４／Ｔ８比值及ＮＫ细胞含量升高有关。     

黄芪针和药饼灸对荷瘤小鼠免疫功能的影响

目的　观察针灸对免疫功能的影响。方法　将C2 6小鼠结肠癌细胞株移植接种建立实验模型的 35只荷瘤小鼠 ,随机分为 5组 ,生理盐水对照组、5 FU组、黄芪针组、药饼灸组、药饼灸 +黄芪针组。由接种瘤细胞第 2日开始进行各组处理。治疗结束后 ,获取小鼠脾脏单个细胞 ,采用流式细胞 (FACS)测定脾淋巴细胞中T细胞亚群、自然杀伤细胞 (NK)及CTL杀伤功能。结果　药饼灸组、黄芪针组、药饼灸 +黄芪针组CD3、CD4、CD4 CD8比率、NK细胞、CTL杀伤活性显著高于 5 FU组 ,P <0 .0 1。结论　药饼灸、黄芪注射液对单用 5 FU治疗所致免疫力低下的荷瘤小鼠的免疫功能具有正向调节作用     

益肺抗瘤饮对肺癌转移及免疫功能的影响

目的：探讨益肺抗瘤饮对肺癌转移的换制作用及其对免疫功能的影响。方法：（１）观察３组（９４例）肺癌患者远处转移灶，用放射免疫测定法测定患者的ＮＫ细胞、ＯＫＴ３、ＯＫＴ４、ＯＫＴ８值，并分比较分析；（２）运用Ｃ５７小鼠Ｂ１６黑素瘤模型观察益肺抗瘤饮对肺转移灶数的影响，并用免疫组织化学法观察转移灶周围Ｔ４、Ｔ８淋巴细胞的改变。结果：益肺抗瘤饮具有降低肺癌患者远处转移和减少Ｂ１６黑色素瘤肺转移的作用，而且