MRI在新生儿缺氧缺血性脑病临床诊断及预后评估中的应用观察

目的 探究磁共振成像(MRI)在新生儿缺氧缺血性脑病(HIE)临床诊断及预后评估中的应用价值.方法 回顾性分析2017年1月至2019年6月我院68例HIE患儿临床资料,患儿在治疗前后均行MRI检查.比较不同临床分度HIE患儿MRI影像学评分差异,并使用Spearman秩相关系数法评估HIE患儿MRI影像学评分与临床分...     

新生儿缺氧缺血性脑病的临床及头颅CT特点分析

目的: 探讨新生儿缺氧缺血性脑病(hypoxia-ischemia encephalop,HIE)的临床与CT的关系。方法: 分析73例新生儿HIE的临床与CT的特点。结果: 73例新生儿HIE临床分轻度23例,中度35例,重度15例;CT分轻度13例,中度41例,重度19例;两者有明显相关性(P<0.005)。结论: 在评估新生儿HIE时,应根据临床分度与CT分度综合分析。     

脑干听觉诱发电位和闪光视觉诱发电位检测在新生儿缺氧缺血性脑病中的应用价值

目的探讨脑干听觉诱发电位(BAEP)和闪光视觉诱发电位(FVEP)检测在新生儿缺氧缺血性脑病(HIE)中的应用价值。方法选择2016年5月至2017年6月新乡市中心医院收治的确诊为缺氧缺血性脑病的新生儿40例为HIE组,另选择同期正常足月新生儿20例作为对照组,分别于出生后20 d对2组新生儿进行BAEP和FVEP检测,并测定Ⅴ波阈值。结果 HIE组新生儿BAEP中Ⅰ、Ⅲ、Ⅴ波的潜伏期及Ⅰ~Ⅲ、Ⅲ~Ⅴ、Ⅰ~Ⅴ峰间期显著长于对照组(P<0.05),HIE组新生儿BAEP中Ⅰ、Ⅲ、Ⅴ波的波幅显著低于对照组(P<0.05),HIE组新生儿BAEP中Ⅴ波阈值显著高于对照组(P<0.05),HIE组新生儿FVEP主波潜伏期显著长于对照组(P<0.05)。结论 BAEP和FVEP联合检测可以早期准确评估HIE新生儿脑损伤及听力损伤程度,对新生儿HIE的早期诊断、病情评估及指导治疗具有重要意义。     

地塞米松,胞二磷胆碱治疗新生儿缺氧缺血性脑病35例疗效观察

新生儿缺氧缺血性脑病(HIE)是围生期新生儿常见的颅内病变。我院采用地塞米松、胞二磷胆碱辅助治疗35例取得满意疗效,现报告如下。1　临床资料1.1　一般资料　1996年1月～1999年5月共收治足月新生儿HIE62例,男42例,女20例,均于生后0.5h～7d入院。其中24h内入院者44例(70.9%)。生后阿氏评分47例(75.8%),出生1min评分<3分、5min<6分43例(91.5%)。窒息原因:宫内窘迫23例,脐带绕颈15例,难产10例,胎膜早破8例,妊高征5例,前置胎盘1例。1.2　临床与CT诊断标准及分度　均符合1996年杭州会议修订标准[1]。62例HIE临床表现依次为反应差32例,嗜睡2…     

纳络酮治疗新生儿缺氧缺血性脑病疗效观察

一般资料：2001年1月-2002年12月诊断新生儿重度窒息及HIE(无论轻重)的患儿，其中重度窒息诊断标准为：生后1分钟APgar评分≤3分或5分钟评分≤5分；HIE诊断标准根据1997年中华医学会儿科学会新生儿组制定的新生儿缺氧缺血性脑病诊断依据和临床分度，确诊HIE77例，其中2002年组37例，     

异体光量子血治疗新生儿缺氧缺血性脑病疗效观察

新生儿缺氧缺血性脑病(HIE)是新生儿死亡及儿童神经系统发育障碍的重要原因。我院采用异体光量子血(UBI)治疗HIE,疗效满意,现报告如下。1　临床资料一般资料　我院于1995年1月至1998年1月收治HIE94例,均符合1989年济南会议制定的HIE临床诊断及分类标准,1996年6月以前的46例为对照组:男29例,女17例;早产儿3例,足月儿43例;入院日龄(2.95±2.45)d;体质量<2500g4例,2500～4000g36例,>4000g6例;出生1minApgar评分:0～3分28例,4～7分18例。1996年6月以后的48例为治疗组:男30例,女18例;早产儿4例,足月儿44例;入院日龄(2.97±2.48)d;体质量<2…     

新生儿缺氧缺血性脑病血清NSE和VILIP-1水平变化及意义

目的 探讨新生儿缺氧缺血性脑病(HIE)血清神经元特异性烯醇化酶(NSE)和视椎蛋白样蛋白1(VILIP-1)水平变化及意义.方法 选取2012年3月至2015年4月在郑州市儿童医院住院治疗的136例HIE患儿,根据病情分为轻度(n=58)、中度(n=46)、重度(n=32),同期,选取正常新生儿45例作为对照组,HIE患儿分别于出生后第24小时(T0)、48小时(T1)、72小时(T2)和7天(T3)时,对照组于T0时,检测血清中NSE和VILIP-1水平,HIE患儿和对照组均于T0时,进行新生儿行为神经测定(NBNA)评分.结果 HIE患儿T0时血清NSE和VILIP-1水平分别为(28.82 ±6.34)、(0.91±0.26) μg/L,均高于对照组的(13.94±2.82)、(0.38 ±0.15) μg/L,NBNA评分为(33.83±2.25)分,低于对照组的(38.24±2.41)分,差异均有统计学意义(P＜0.05);Pearson相关分析显示,HIE患儿T0时血清NSE水平与VILIP-1水平呈正相关(r=0.612,P＜0.05),血清NSE和VIL-IP-1水平均与NBNA评分呈负相关(r=-0.481、-0.440,P＜0.05);与轻度相比,中度和重度患儿T0～3时血清NSE和VILIP-1水平均升高,且重度均高于中度,差异均有统计学意义(P＜0.05).结论 HIE患儿血清NSE和VILIP-1水平升高,动态监测血清NSE和VILIP-1水平对HIE早期诊断、病情判定及治疗效果评估具有重要意义.     

1,6-二磷酸果糖治疗中、重度HIE伴心肌损害疗效观察

新生儿缺氧缺血性脑病 (HIE)是新生儿围产期窒息后多脏器损害的重要部分 ,而中、重度HIE常伴有心肌损害 ,可引起新生儿死亡或致残。 2 0 0 1～ 2 0 0 2年间 ,对6 4例HIE患儿 ,作者在传统治疗的基础上 ,加用 1 ,6 -二磷酸果糖 (FDP) ,疗效显著 ,现报告如下。1 资料与方法一般资料　 6 4例中、重度HIE患儿 ,均符合 1 997年制定的HIE标准并经头颅CT扫描确诊(1 ) 。全部病例伴有心肌损害。心肌酶谱 (CK -MB)均增高 ,至少有一项临床表现或心电图异常。临床表现有心音低钝或 (和 )心率减慢 (心率 <1 0 0次 /分 ) ,重者有心力衰竭 ,心电…     

新生儿缺氧缺血性脑病的CT诊断体会

新生儿缺氧缺血性脑病 (hypoxic ischemic en-cephalopathy,HIE) ,系指围产期窒息所致新生儿脑部缺氧缺血性损伤 ,是引起脑瘫、智力低下及癫痫疾病的重要因素 ,是新生儿常见病以及死亡原因之一。新生儿缺氧缺血性脑病 (HIE)的早期诊断对临床治疗和预后评估有重要的指导意义。HIE的表现征象诊断 ,是目前专家认可的检查方法和较可靠的手段。现将 38例经行头颅 CT检查诊断为新生儿缺氧缺血性脑病 (HIE)的诊断体会进行分析报道如下。1　资料与方法　　选择 1 998年 8月～ 2 0 0 1年 2月因窒息、惊厥等住院的新生儿 ,经行头颅 CT检查诊断…     

新生儿缺氧缺血性脑病与产科因素关系的分析

新生儿缺氧缺血性脑病(HIE),是胎儿窘迫或新生儿窒息所致脑缺氧而引起的脑部损伤,是新生儿致残,致死的主要原因之一,为预防和减少HIE的发生,对近两年来在我院产科分娩的25例HIE新生儿的产科因素进行分析,现报道如下。1　临床资料1.1　一般资料:1998年1月至1999年12月我院共分娩产妇1814例,新生儿1822例,其中双胎分娩8例,顺产1487例,剖宫产301例,阴道助产术26例,高危妊娠36例,胎儿窘迫146例,新生儿窒息58例,重度窒息16例,HIE25例,发生率1.37%。HIE的诊断标准按《实用新生儿学》的诊断标准(1)。1.2　HIE的相关因素:HIE的直接病因是胎…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.