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1.
To investigate the role of platelet membrane glycoprotein (GP) Ib/Ⅸ/Ⅴ complex and its subunit GP Ibα in patients with hemorrhagic thrombopathy (HT), the expressions of GP Ib/Ⅸ/Ⅴ complex and GP Ibα,defined as mean fluorescence intensity (MFI), were assessed by flow cytometry. The maximum aggregation of platelet was determined by turbidity method. These indicators were compared among 68 HT patients with the presenting complaint of hemorrhage, 33 well-controlled HT patients and 32 normal healthy subjects. The results showed that the MFI of GP Ib/Ⅸ /Ⅴ complex and GP Ibα was markedly lower in HT patients with current hemorrhage than that in the healthy subjects, with difference being statistically significant (P<0.05). There was no significant difference in the expressions of GP Ib/ Ⅸ/ Ⅴ complex and GP Ibα between well-controlled HT patients and normal healthy subjects (P>0.05). It was concluded that the expression of GP Ib/Ⅸ /Ⅴ complex, the receptor of thrombin and von Willebrand factor, was down-regulated in HT patients with current hemorrhage, which might result in the dysfunction of platelet aggregation and recurrence of HT.  相似文献   

2.
To investigate whether peroxisome proliferators-activated receptor-γ (PPARγ) ligand Troglitazone can reduce endothelial injury and activation during storage of harvested saphenous vein grafts. Segments of human saphenous vein graft were collected from 9 patients undergoing coronary bypass surgery and then divided into two equal parts of control and test specimens, were stored in ei- ther heparinized blood (control group) or heparinized blood containing 20 μmol/L troglitazone (test group) for 1 h at room temperature. Tissue distribution and protein expression of VCAM-1, ICAM-1, and endothelial nitric oxide synthase (eNOS) were compared using immunohistochemistry and West- ern blot analysis. Myeloperoxidase (MPO) activity, a marker of neutrophil sequestration in human saphenous vein grafts, was also measured in each group. The expression of ICAM-1 (753±132 versus 7201±934; P<0.01),VCAM-1 (3731±294 versus 8292±793; P<0.01), and MPO activity (1.52±0.42 U/g,5.04±1.26 U/g P<0.01) were significantly lower in test group. In contract, eNOS expression (7983±834 versus 3989±1008; P<0.01) was significantly higher in test group. PPARγ ligand troglita- zone might reduce endothelial injury during the storage period of human saphenous vein grafts.  相似文献   

3.
To investigate whether peroxisome proliferators-activated receptor-γ (PPARγ) ligand Troglitazone can reduce endothelial injury and activation during storage of harvested saphenous vein grafts. Segments of human saphenous vein graft were collected from 9 patients undergoing coronary bypass surgery and then divided into two equal parts of control and test specimens, were stored in ei- ther heparinized blood (control group) or heparinized blood containing 20 μmol/L troglitazone (test group) for 1 h at room temperature. Tissue distribution and protein expression of VCAM-I, ICAM-I, and endothelial nitric oxide synthase (eNOS) were compared using immunohistochemistry and Western blot analysis. Myeloperoxidase (MPO) activity, a marker of neutrophil sequestration in human saphenous vein grafts, was also measured in each group. The expression of ICAM-1 (753±132 versus 7201±934; P〈0.01) , VCAM-1 (3731±294 versus 8292±793; P〈0.01), and MPO activity (1.52±0.42 U/g, 5.04±1.26 U/g P〈0.01) were significantly lower in test group. In contract, eNOS expression (7983±834 versus 3989±1008; P〈0.01) was significantly higher in test group. PPARγ ligand troglitazone might reduce endothelial injury during the storage period of human saphenous vein grafts.  相似文献   

4.
高糖对人脐静脉内皮细胞GSH-PX活力、NOS及ICAM-1表达的影响   总被引:10,自引:0,他引:10  
目的:探讨体外培养条件下高糖状态对血管内皮细胞的影响。方法:培养人脐静脉内皮细胞株ECV3 0 4,分为对照组和高糖组,用分光光度比色法检测谷胱甘肽过氧化物酶(GSH PX)活力,RT- PCR法检测细胞间粘附分子1(ICAM- 1)mRNA水平,免疫细胞化学法观察内皮细胞型一氧化氮合酶(eNOS)和诱导型一氧化氮合酶(iNOS)的蛋白表达。结果:高糖组GSH- PX活力明显下降,eNOS、iNOS蛋白表达明显增高,ICAM- 1mRNA水平也显著上升。结论:在较短时间内糖浓度升高可引起血管内皮细胞GSH PX活力下降,ICAM -1mRNA、eNOS和iNOS蛋白表达增高,对糖尿病血管病变早期进展起一定的作用。  相似文献   

5.
张建初  夏蕾  杨卫兵  陶晓南  白明 《广东医学》2006,27(11):1672-1674
目的 观察急性肺血栓栓塞(FFE)患者溶栓前后血浆一氧化氮(NO)水平的变化,以及对人脐静脉内皮细胞(HUVEC)内皮型一氧化氮合酶(eNOS)基因表达的影响。方法 采用硝酸还原酶法测定PTE患者溶栓前及溶栓4h后血浆NO浓度,溶栓前后患者血浆孵育HUVEC,RT—PCR检测eNOS mRNA的表述变化,western blot检测eNOS蛋白的表达变化。结果 急性肺血栓栓塞溶栓前后患者血浆NO水平均高于正常人血浆NO水平,且差异均有显著性(P〈0.05);而患者溶栓前后血浆NO水平相比,差异无显著性(P〉0.05);患者溶栓前后血浆孵育HUVEC,随时间变化,其eNOS mRNA及蛋白表达均逐渐增强,相同时间点两组eNOS mRNA及蛋白的表达差异均无显著性(P〉0.05),但均高于正常血浆孵育组(P〈0.05)。结论 PTE患者血浆NO水平显著上升,溶栓后4h内其NO水平无明显变化,P1E患者血浆能够上调HUVEC的eNOS基因表达。  相似文献   

6.
目的 对代谢综合征(membolic syndrome,MS)患者循环内皮细胞(circulation endothelial cells,CECs)、血浆一氧化氮合酶和一氧化氮(nitric oxide synthase/nitric oxide,NOS/NO)系统及血浆内皮素(en-dothelin,ET)进行测定,探讨MS对此三方面的影响.方法 根据MS诊断标准选取34例患者作为MS患者组和47例正常人群作为健康对照组,分别计数CECs、测定血浆NOS和NO水平及ET水平.结果 ①与对照组相比较,MS患者组CECs](3.11±0.69)vs(9.47±1.15),P=0.0011显著升高.②与对照组相比较,MS患者组NOS[(28.17±3.11)μmol/L vs(34.28±1.97)μmol/L,P=0.0361和No [(58.33±2.49)μmol/L vs(70.03±1.09)¨mol/L.P=0.0141均显著降低.③与对照组相比较,MS患者组ET[(56.35±10.15)vs(126.58±12.37),P=0.001]显著升高.④MS与CECs(P=0.001)和ET(P=0.001)呈正相关,而与NOS(P=0.011)和NO(P=0.006)呈负相关;CECs和ET(P=0.001)呈正相关而与NOS(P=0.035)和No(P=0.024)呈负相关.结论 MS患者血浆NOS和NO水平显著降低,同时致CECs增多和血浆ET水平升高.  相似文献   

7.
ABSTRACT Objective: To investigate the effect of Xiaoyu Zhixue Tablet (消瘀止血片, XYZXT) on the expression of platelet membrane glycoprotein (GP) Ⅰ b/Ⅸ/Ⅴ complex and GP Ⅰ b α in patients with chronic renal failure (CRF) in early metaphase. Methods: Fifty-one patients with CRF in early metaphase (treated group) were treated with XYZXT, 3 months as the course of treatment for 2 courses. The previous therapies remained unchanged. Flow cytometry was used to assess the expression of platelet GP Ⅰb/Ⅸ/Ⅴ complex and GP Ⅰb α in patients with CRF, and turbidity method was used to determine the platelet maximum aggregation rate (MAR), meanwhile the renal function was measured. The final data were compared with those before the treatment, and with those in the normal control group (31 healthy subjects). Results: Compared with the normal control group, expressions of GP Ⅰ b/Ⅸ/Ⅴ complex and GPⅠb α, and platelet MAR in CRF patients were significantly lower (P=0.007,P=0.001,P=0.009) before the treatment; after the treatment with XYZXT, the above indexes in CRF patients were remarkably increased (P=0.033,P=0.026, P=0.045), but still lower than those in the normal control group, however, it was not statistically significant. Conclusion: (1) The expression of GP Ⅰ b/Ⅸ/Ⅴ complex in CRF patients of early metaphase was decreased, which lead to platelet aggregation dysfunction. This might be one of the reasons for the hemorrhagic trend in CRF. (2) XYZXT was able to upgrade expressions of GP Ⅰb/Ⅸ/Ⅴ complex and GPⅠb α in CRF patients, improve platelet function and clown-regulate platelet activation in patients with CRF.  相似文献   

8.
Objective: To observe the effect of Xiaoyu Zhixue tablet (XYZXT) on the expression of platelet membrane glycoproteins in patients with hemorrhagic thrombopathy, and to explore its possible mechanism. Methods; The total of 148 patients with hemorrhagic thrombopathy were randomly divided into two groups, the traditional Chinese medicicne (TCM) group (n = 98) treated with XYZXT and the Western medicine (WM) group (n = 50) treated with adrenosin, vitamins C, K and P, both for 6 months. The therapeutic effect and the recovery rate of platelet aggregation in the two groups were observed. And platelet membrane glycoprotein (GP) Ib/IX, GPII b/IIIa complexes, GPIb, GPIIb, GPIIIa and P-selectin were analyzed by flow cytometry in both groups before and after treatment and also in 34 normal healthy subjects.Results: The total effective rate of hemostasis was 89.8% in TCM group and 54.0% in the WM group (X 2=45.83,P<0.01), and the recovery rate of platelet aggregation was 72.4% and 4.0% respectively (X 2 = 62.06,P<0.01). The fluorescence intensity of GP I b/IX, GP II b/III a complexes, GPIb, GP IIIa and P-selectin were lower in both groups before treatment than those in the healthy subjects. Expression of above-mentioned marks was elevated in TCM group after 6 months’ therapy, which was insignificantly different as compared with the healthy subjects (P>0.05) and higher than those in the WM group (P<0.05).Conclusion: One of the mechanisms in treating hemorrhagic thrombopathy with XYZXT is that it could regulate the expression of GP I b/IX, GPII b/IIIa complexes, GP I b, GPIIIa and P-selectin at the level of receptor protein. Supported by the National Natural Science Foundation of China (No. 30171194)  相似文献   

9.
刘亚平  姚康  徐标 《现代医学》2003,31(3):163-164
目的 通过测定糖尿病患者血小板一氧化氮合酶 (NOS)活性 ,探讨糖尿病患者血小板激活的机制。方法 晨取 16例 2型糖尿病患者外周静脉血 ,利用凝胶色谱柱法收集血小板 ,同位素二步色谱法测定血小板NOS活性。 8例健康成人作为对照。结果 糖尿病患者血小板NOS活性明显低于正常人 (P <0 0 1) ,组胺刺激后仍低于正常人 (P <0 0 5 )。结论 血小板NOS活性降低是糖尿病患者血小板活性增高的重要原因  相似文献   

10.
目的通过检测低氧状态血管内皮细胞一氧化氮合酶(eNOs)及细胞间黏附因子(ICAM1)表达的变化.探讨低氧对血管内皮细胞功能的影响。方法采用逆转录聚合酶链式反应(RT-PCR)及Western blot测定低氧条件下不同时间点大鼠动脉内皮细胞中eNOS、ICAM-1 mRNA及蛋白质的表达变化情况。结果同常氧组相比,低氧1h时eNOS、IcAM-1 mRNA及蛋白表达无变化.而6、12、24h时eNOS、ICAM-1mRNA及蛋白表达明显升高(P〈0.05),但eNOS mRNA与ICAM-1mRNA的表达变化在同一时间点并不一致。结论低氧状态下血管内皮细胞eNOS与ICAM-1表达升高,可能参与了低氧性肺血管疾病的发病机制。  相似文献   

11.
目的观察出血性血小板病患者的血小板膜糖蛋白(GP)Ib/IX/V复合物及其组分GP Ibα的表达。方法采用流式细胞术检测68例出血性血小板病患者在出血期的血小板GPIb/IX/V复合物和GP Ibα的表达,采用比浊法检测其血小板最大聚集率(MA),并与33例出血控制者及32例正常健康者比较。结果出血性血小板病患者在出血期间GPIb/IX/V复合物、GP Ibα表达明显低于正常组(P均〈0.01),出血控制者的GPIb/IX/V复合物、GP Ibα表达与正常组比较无显著性差异(P均〉0.05)。结论血小板GPIb/Ⅸ/V复合物作为凝血酶和血管性血友病因子的受体,在出血性血小板病患者体内的表达下降,导致血小板聚集功能障碍,可能是本病反复发作出血倾向的重要原因之一。  相似文献   

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