冠心舒抗炎症作用实验研究

实验研究表明,腹腔注射冠心舒200mg/kg 对大鼠足跖肿胀实验性炎症有明显的抑制作用,腹腔注射冠心舒100mg/kg6天,对大鼠棉球肉芽增生性炎症也有明显的抑制作用。实验证实,冠心舒的抗炎症作用机理可能与肾上腺,胸腺及巨噬细胞的吞噬机能有关,回顾酸性粘多糖抗炎作用的有关理论,认为某些抗炎药物防治动脉粥样硬化作用的研究尚需进一步深入。     

赤雹根总皂苷对AA大鼠足跖部炎症反应的作用

目的:观察赤雹根总皂苷(TSTDR)对佐剂性关节炎(AA)大鼠足跖部炎症反应的治疗作用。方法:雄性SD大鼠60只随机分为正常对照组,模型组,TSTDR低、中、高剂量组(40、80、160mg/kg/d)和雷公藤多苷组(12mg/kg/d),每组10只大鼠。大鼠右后足跖部皮下注射弗氏完全佐剂0.1ml建立AA大鼠模型,成模后各用药组大鼠分别灌胃给予相应药物21d。分别于给药前及给药后5、9、13、17、21d测定各组大鼠左后足肿胀度,并观察大鼠足跖部的病理变化。结果:与模型组相比,TSTDR高、中、低剂量组大鼠足肿胀度分别于给药5d、9d、13d明显下降(P<0.01);TSTDR高、中剂量组大鼠足跖部的炎症浸润明显减轻。结论:TSTDR对AA大鼠足跖部炎症反应具有明显的抑制作用。     

身痛逐瘀胶囊对大鼠Freund's完全佐剂性关节炎动物模型的影响

目的:观察身痛逐瘀胶囊对Freund's完全佐剂性关节炎模型及继发病变程度的影响.方法:Freund's完全佐剂复制关节炎模型,容积测量法观察模型大鼠足跖肿胀变化情况及尾、耳、前足等部位的继发性病变,采用分级计分法对续发性炎症反应进行量化评估.结果:表明身痛逐瘀胶囊不仅对大鼠(右)右足跖注射Freund's完全佐剂所致的早期炎症反应(关节肿胀)有抑制作用,同时对另侧(左后足)因迟发型超敏反应引起的足肿胀也有一定的抑制作用,对大鼠的尾部、耳廓继发性炎症反应积分与模型组比较有显著性差异(P＜0.05).结论:提示身痛逐瘀胶囊对佐剂性关节炎及继发性损害有一定程度的改善作用.     

复方马钱子凝胶镇痛及对IL-6影响作用的研究

目的:通过对大鼠炎症疼痛模型痛阈值的观察,以及在血清中检测白细胞介素-6I(nterleukin-6I,L-6)的表达,探讨复方马钱子凝胶的镇痛及抑制炎症反应作用及机理。方法:构建甲醛注射大鼠足跖引起的炎症疼痛模型,测量不同时间大鼠足跖疼痛阈值,应用酶联免疫吸附(Enzyme-linked immunosorbent assay,ELISA)方法检测各组大鼠血清中IL-6的表达水平。结果:复方马钱子凝胶可明显提高甲醛致痛大鼠的痛阈值(P<0.01),抑制血清中IL-6表达水平(P<0.01)。结论:复方马钱子凝胶治疗软组织炎症疼痛损伤,对IL-6的抑制和减轻疼痛的作用,明显优于基质对照组,与扶他林阳性对照组作用相当,提示复方马钱子凝胶外敷,治疗实验性炎症和疼痛方面具有良好的抑制炎症反应和镇痛作用。     

督脉加药发泡疗法治疗大鼠佐剂性关节炎的研究

目的观察督脉加药发泡疗法对大鼠佐剂性关节炎(AA)的疗效,并初步探讨其作用机理。方法采用大鼠足跖皮内注射福氏完全佐剂建立AA模型,采用雷公藤膏、斑蝥膏、斑蝥雷公藤膏督脉穴位贴敷(发泡)疗法,观察足跖肿胀度、足爪组织病理学变化、脾脏指数、血清和足跖炎症组织中的PGE2含量。结果斑蝥膏组、斑蝥雷公藤膏组可有效抑制AA大鼠足跖肿胀度和足爪组织病理学变化,尤其以斑蝥雷公藤膏组为更显著。发泡疗法可提高大鼠脾脏指数值以及血清中的PGE2含量,而足跖炎症组织中PGE2含量各组间无明显差异。结论督脉发泡疗法对AA具有良好的抗炎消肿作用,在发泡剂中添加雷公藤后疗效更佳,其机理可能与免疫调节有关,与血清和足跖炎症组织中的PGE2含量变化无关。     

从动物实验观察颈痛宁冲剂的抗炎作用

从动物实验观察了颈痛宁冲剂对角叉菜胶、甲醛致炎大鼠足跖肿胀与炎症部位PGE的影响。结果表明,颈痛宁2.4g/kg与3.6g/kg剂量组能明显地抑制角叉菜胶、甲醛所致大鼠足跖肿胀,提命小鼠痛反应阈值,对炎症部位的PGE无影响,其抗炎作用不同于消炎痛抑制PGE的合成与释放。     

洋辣子水剂的抗炎及抗慢性胃溃疡作用

本实验研讨了100％洋辣子水剂及0.001％消炎痛水剂对实验组和对照组的抗炎抗慢性胃溃疡作用,洋辣子水剂(2g/100g)对鹿角菜(Carrageenin)所致大鼠足跖急性炎症及佐剂所致大鼠关节炎均有明显的抑制作用,也抑制蛋清所致大鼠足跖急性炎症。洋辣子水剂对醋酸所致大鼠慢性胃溃疡也有较明显的抑制作用。     

嗽宁口服液抗炎解热作用的实验研究

目的：观察嗽宁口服液的抗炎、解热作用。方法：采用植入脱脂棉球、皮下注射角叉菜胶制作大鼠炎症模型，皮下注射酵母菌制作大鼠发热模型，观察嗽宁口服液对炎症大鼠肉芽干重、足跖厚度及体温的影响。结果：嗽宁口服液能明显降低大鼠肉芽组织干重、足跖厚度及体温。结论：嗽宁口服液有一定的抗炎、解热作用。     

喘康颗粒抗炎作用研究

目的 :研究喘康颗粒抗炎作用 ;方法 :采用二甲苯致小鼠耳廓肿胀、角叉菜胶致小鼠足跖肿胀、蛋清致大鼠足跖肿胀、醋酸致小鼠腹腔毛细血管通透性增加、小鼠棉球肉芽肿 ,对小鼠炎症组织炎症介质丙二醛及肝组织LPO含量的测定 ,进行抗炎作用试验 ,并对结果进行统计学检验 ;结果 :喘康颗粒较显著抑制二甲苯小鼠耳廓肿胀、非常显著抑制角叉菜胶致小鼠足跖肿胀、对蛋清性大鼠足跖肿胀有一定抑制 ,极显著抑制HAC致小鼠腹腔毛细血管通透性增高、显著抑制小鼠棉球肉芽肿。显著降低小鼠炎症组织炎症介质His、PGE、MDA含量 ;结论 :喘康颗粒具有较强的抗炎作用 ,显著降低炎症组织PGE和His含量 ,揭示喘康颗粒抗炎作用是其平喘作用机理之一。     

妇炎消胶囊抗炎作用及其机制

目的观察妇炎消胶囊的抗炎作用，并初步探讨其抗炎作用机制。方法用二甲苯所致小鼠耳肿胀、角叉菜胶所致大鼠足跖肿胀及放置塑料环所致大鼠子宫炎症模型。观察小鼠耳肿胀程度、大鼠足跖肿胀程度及大鼠子宫炎症肿胀程度。结果妇炎消胶囊能剂量依赖性减轻二甲苯所致小鼠耳肿胀程度（P〈0．05，P〈0．01）、减轻角叉菜胶所致大鼠足跖肿胀程度（P〈0．05，P〈0．01）和减轻大鼠子宫炎症肿胀程度（P〈0．05，P〈0．01）。结论妇炎消胶囊对炎性肿胀程度具有明显的抑制作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.