X射线对肺癌细胞株A549的P57kip2和TGF-β1蛋白表达的影响

目的 观察不同剂量X射线照射后不同时间A549肺癌细胞株中P57^kip2和TGF-β1蛋白表达水平,探讨X射线对p57^kip2和TGF-β1的影响及临床意义。方法 按常规方法培养肺癌细胞株A549,实验分5组,照射组分别给予不同剂量X射线(2、4、8和12 Gy)照射,于不同时间(6、12、24、36和48 h)提取蛋白,采用Western blot 检测p57^kip2和TGF-β1蛋白的表达水平。结果 X射线可导致肺癌细胞P57^kip2蛋白的表达增加,照后12 h达峰值,在2～8 Gy之间,p57随着照射剂量的增加而升高,12 Gy时,其含量较4～8 Gy减少。不同剂量和不同时间P57^kip2蛋白的表达,差异有统计学意义(P＜0.05),TGF-β1在照后6h达峰值,之后迅速下降,各不同剂量和不同时间水平之间差异有统计学意义(P＜0.05)。结论 X射线照射可以上调肺癌细胞株A549的p57^kip2和TGF-β1蛋白表达,两者有一定的相关性,且在一定剂量范围内呈剂量依赖性,可以反映肺癌细胞损伤的程度。     

滑膜骨软骨瘤病的x线和CT表现

滑膜骨软骨瘤病起源于滑膜下层的结缔组织病变,关节滑膜软骨性化生,在滑膜面下形成多发的软骨性结节,突向关节腔内,这些软骨体结节可以游离于关节腔内,由关节滑液供给营养,并可钙化或骨化。本病较少见,主要表现为关节疼痛、肿胀、活动受限。X线平片结合CT检查可以大大提高该病诊断的准确率。本研究对我院手术和病理证实的10例滑膜骨软骨瘤病患者临床资料进行回顾性分析,现报告如下。     

明胶溶液和右旋糖酐-70对血小板膜糖蛋白Ⅰb /Ⅰx和血管性假性血友病因子的影响

为探讨在硬膜外阻滞下行胆囊切除术的病人术中输注明胶溶液和右旋糖酐-70后对血小板膜糖蛋白Ⅰb/Ⅰx(GPⅠb/Ⅰx)、血管性假性血友病因子(vWF)和血小板粘附试验(PAdT)的影响以及相互关系,将105例病人随机分为4组A组术中输注生理盐水;B组输注右旋糖酐-70;C组输注尿联明胶;D组输注改良明胶.于输注前、输注后2h、3h采集静脉血,分别测定GPⅠb/Ⅰx、vWF及PAdT.结果显示B组在输注3h后vWF明显低于其它3组(P<0.01),B组GPⅠb/Ⅰx输注液体3h后比输注前也有显著降低(P<0.01),与其它3组比较也有非常显著的差异(P<0.01).A组和C组PAdT有显著的增高(P<0.05).提示改良明胶和尿联明胶在临床上可能比右旋糖酐-70使用更大的量而不造成出血倾向,明胶类液体较晶体液在术中更能稳定血小板,在某些方面可能有助于止血功能的稳定.     

Views of Australian and New Zealand radiation oncologists and registrars about evidence-based medicine and their access to Internet based sources of evidence

In order to determine the views of Australian and New Zealand (ANZ) radiation oncologists and registrars about evidence-based medicine (EBM) and their access to, and use of, Internet-based EBM resources such as MEDLINE and the Cochrane Library, a self-administered postal survey was sent out in December 2000. A total of 191 of 243 practicing ANZ radiation radiation oncologists (138) and registrars (53) responded (78.6% response rate). The main outcome measures were: (i) views about EBM and its promotion; (ii) previous EBM training; (iii) extent of Internet access and level of MEDLINE use and access to online medical journals; (iv) awareness of the Cochrane Library and other EBM resources; and (v) self-assessment of common EBM technical terms. The results of the survey found that most (82.7%) indicated a positive view of themselves towards the current promotion of EBM. However, significantly fewer perceived their colleagues were as positive (chi2 = 35.2, d.f. = 1, P < 0.001). Most (84.8%) considered research findings 'useful' in day-to-day management of patients. The majority (70.2%) of respondents never had attended an EBM course; however, most indicated interest in such training. The majority (73.8%) reported having accessed MEDLINE or another bibliographic database in the previous month. Seventy-seven respondents (40.3%) reported that, in the past month, a literature search/article had influenced their practice. Only a minority (28.3%) used the Cochrane Library. Self-assessment of most of the 12 EBM terms was generally high. For radiation oncologists, past attendance at an EBM course was significantly associated with a better overall self-assessment of these 12 terms (chi2 = 10.59, d.f. = 1, P = 0.001). These findings reveal strong support for EBM among ANZ radiation oncologists and registrars with most regularly accessing the Internet for evidence. However, lack of awareness of some relevant EBM related resources, particularly the Cochrane Library, remains a challenge. Our findings invite a concerted effort by those promoting EBM to ensure clinicians have both the skills and knowledge to practice EBM.     

血液透析分流通道狭窄和闭塞的介入治疗

目的 总结35例自体血管瘘道（桡动脉与头静脉侧端吻合）机能不全和闭塞的血管造影诊断及介入治疗结果。方法 对35例自体血管瘘道机能不全和闭塞患者行血管造影。26例血栓形成闭塞患者行溶栓治疗,15例行经皮血管成形术（PTA）。结果 初次造影结果,9例单纯血管狭窄,26例血栓闭塞。溶栓即时开通率为88．4％（23／26）,其中13例伴血管狭窄中7例及单纯性狭窄中8例共15例进行了PTA治疗,成功12例,失败3例。追踪观察6例溶栓后伴狭窄未处理及PTA失败者（3例）。1个月内7例发生再阻塞,3个月内全部再阻塞。结论 血管造影及介入治疗对血液透析分流通道机能不全和闭塞具有重要意义。     

基于物联网的大型生物样本库全流程管理

生物样本库对临床医学和生命科学有重要的意义。为解决样本库管理中无法实时的对样本状态进行监控和记录的问题,优化管理流程,作者利用物联网和计算机技术开发了一套新的样本管理系统。该系统不仅满足了日常管理需要,同时能记录样本信息与样本管理流程,减少了操作人员的工作量,避免手工记录偏差,实现样本库管理的自动化。     

复方活血汤对放射性损伤小鼠造血细胞bcl-2\bax\bcl-X-L基因表达的作用

目的探讨放射性损伤骨髓衰竭的机理及防护对策。方法用Ｎｏｒｔｈｅｒｎｂｌｏｔ技术检测了６０Ｃｏγ射线８．０Ｇｙ照射小鼠（对照组）及照射后立即给复方活血汤治疗小鼠（中药组）的骨髓有核细胞ｂｃｌ－２、ｂａｘ、ｂｃｌ－ｘＬ基因表达。结果放射损伤后第３，７天，对照组骨髓有核细胞ｂｃｌ－２／ｂａｘ值减小、ｂｃｌ－ｘＬ不表达。中药组与之相反，ｂｃｌ－２／ｂａｘ值明显增大、ｂｃｌ－ｘＬ明显表达。受照后第７天两组脾脏单个核细胞ｂｃｌ－２／ｂａｘ值与骨髓有核细胞的表达一致。结论复方活血汤对放射损伤骨髓造血细胞增殖周期调控基因ｂｃｌ－２、ｂａｘ、ｂｃｌ－ｘＬ有一定调节作用，保护骨髓造血     

基于荟萃分析和线性微分方程模型研究肺纤维化细胞因子调控通路

目的: 探讨肺纤维化过程中细胞因子的调控网络.方法: 应用荟萃分析筛选Wistar大鼠肺纤维化过程中肺组织的相关细胞因子.通过三次样条插值,采用线性微分方程模型进行网络调控的研究.结果: 参与肺纤维化形成的相关细胞因子为IFN-γ,IL-10,IL-13,IL-18, IL-4, IL-5,IL-6,IL-8,IGF-1, MCP-1, PDGF, TGF-β1, TNF-α, MMP-2, MMP-9, EGF.构建的调控通路中重要的细胞因子节点为IFN-γ,IL-10,IL-13,IL-18, IL-6,IGF-1, PDGF, TGF-β1,TNF-α.结论:肺纤维化细胞因子网络由关键的细胞因子组成,非关键节点的细胞因子可能因致纤维化作用较小或仅参与炎症作用而未参加网络的调控.     

用于室颤节律辨识的多参数融合BP神经网络设计

目的：构建一个用于区分室颤节律和非室颤节律的多参数融合BP神经网络。方法从心电信号中提取出18个特征值,分别标记心电信号的形态分布、高斯性、幅度谱、变异性、复杂度等各方面特征;以这些特征值作为输入向量,构建一个多参数融合BP神经网络进行训练,得到一个用于区分室颤节律与非室颤节律的分类器。结果与结论将构建的BP神经网络分别基于VFDB数据库和CUDB数据库进行实验,辨识准确率分别高达98．61％和95．37％;相较于现有方法,辨识性能显著提高。     

Leukocyte cells identification and quantitative morphometry based on molecular hyperspectral imaging technology

Leukocyte cells identification is one of the most frequently performed blood tests and plays an important role in the diagnosis of diseases. The quantitative observation of leukocyte cells is often complemented by morphological analysis in both research and clinical condition. Different from the traditional leukocyte cells morphometry methods, a molecular hyperspectral imaging system based on acousto-optic tunable filter (AOTF) was developed and used to observe the blood smears. A combined spatial and spectral algorithm is proposed to identify the cytoplasm and the nucleus of leukocyte cells by integrating the fuzzy C-means (FCM) with the spatial K-means algorithm. Then the morphological parameters such as the cytoplasm area, the nuclear area, the perimeter, the nuclear ratio, the form factor, and the solidity were calculated and evaluated. Experimental results show that the proposed algorithm has better performance than the spectral based algorithm as the new algorithm can jointly use the spatial and spectral information of leukocyte cells.     

基于表面增强拉曼光谱的肝病血清检测技术研究

目的：通过比较正常人与慢性乙肝、肝硬变和肝癌患者血清之间的拉曼光谱差异,探索基于表面增强拉曼散射（ SERS）的血清诊断新方法。方法首先制备纳米银溶胶作为活性基底,分别测量30例正常人和48例慢性乙肝、48例肝硬变、46例肝癌患者血清的增强拉曼信号,并进行正交偏最小二乘法判别分析（ OPLS－DA ）。结果正常人与3组肝病患者血清在位移625、725、806、947、1018、1219、1131、1329、1440、1580、1660 cm－1处均有拉曼峰,且峰强弱存在差异;正常人血清在位移1096和1395 cm－1处有强峰,肝病患者血清在位移887 cm－1有强峰。 ROC曲线下面积（AUC）分别为0．981、0．966、0．984。结论初步研究表明血清SERS图谱可作为肝病早期诊断的一种辅助手段。     

US-CT影像融合容积导航技术可重复性的实验研究

目的 探讨US-CT影像融合容积导航技术在肝脏体模应用中的价值.方法 采用包含3条肝静脉及6枚囊肿模型的体外肝脏体模,应用融合导航定位系统将其CT薄层扫描图像导入超声检查仪虚拟导航系统进行影像融合,在囊肿模型的最大截面层,应用matlab软件计算影像重合率.实验共进行2次:1)2名不同资质的操作者应用影像融合容积导航技...     

基于虚拟专用网的互动式影像远程会诊体系的创建与初步应用

目的探讨互动式影像远程会诊系统的创建及其特点。方法下级医院局域网、防火墙[含虚拟专用网(virtual private network，VPN)功能]及不对称数字线路调制解调器(ADSL Modem)1套，联入Intemet，通过贵阳医学院附属医院防火墙接入本院VPN服务器，获得VPN隧道联入本院医学影像存储与传输系统(picture archiving and communication systems，PACS)服务器，使本院影像科诊断终端及下级医院能够共享同一影像数据。结果下级医院影像数据及检查信息能安全、无损的上传到本院PACS保存和管理，并进行会诊后将会诊结果以报告方式返回给下级医院。影像上传速度快，质量优良，操作互动性好。结论基于VPN的互动式远程会诊系统具有安全、快捷、互动性好、操作简便、实用可靠和优良的性价比等优点，值得推广。