妇科活血止痛颗粒对血淤模型大鼠血液流变学的影响

目的 观察妇科活血止痛颗粒对急性血淤模型大鼠血液流变学的影响.方法 雌性SD大鼠50只,随机分为空白对照组,急性血淤模型组,妇科活血止痛颗粒低剂量组(0.23g/kg)、中剂量组(0.46g/kg)和高剂量组(0.92g/kg),每组10只,全部动物以标准饲料喂养,3次/d.对照组和血淤模型组大鼠每日末次喂食后1h以10mL/kg生理盐水灌胃,低、中高剂量组每日末次喂食1h后按0.23、0.46、0.92g/kg剂量给予妇科活血止痛颗粒混悬液灌胃,灌胃容量10mL/kg,共10 d.第10天给药1 h后,除对照组外的其他组动物均皮下注射肾上腺素,冰水浸泡建造急性血淤模型,造模结束18h后,股动脉取血测定不同切变率的全血黏度、血浆黏度、红细胞压积、红细胞聚集指数等指标.结果 血淤模型组大鼠全血黏度、全血还原黏度(低切和中切)明显高于对照组(P＜0.05或P＜0.01);低中剂量组明显低于血淤模型组(P＜0.05或P＜0.01);血淤模型组大鼠红细胞压积、红细胞聚集指数明显高于对照组(P＜0.05或P＜0.01),红细胞压积低、中、高剂量组大鼠均低于血淤模型组(P＜0.05或P＜0.01).红细胞聚集指数积低、中剂量组大鼠均低于血淤模型组(P＜0.05或P＜0.01).结论 妇科活血止痛颗粒对改善大鼠血液流变学状态具有良好作用.     

Influence of Danhong for injection on hemorrheology,thrombus and platelet function in rats

目的 作者简介:王宇翎,女,副教授,责任作者,E-mail:wangyuling1973@126.com观察注射用丹红对血液流变学及血栓、血小板功能的影响.方法 SD大鼠随机分为6组:对照组、血瘀模型组、注射用丹红(2.5、5.0、10.0 g/kg)组及丹参注射液组(5.0 g/kg).制备大鼠急性血瘀模型,观察注射用丹红对大鼠血液流变学和血小板黏附和聚集的影响;采用体外血栓形成试验观察注射用丹红抗血栓形成作用.结果 注射用丹红5.0 g/kg组可明显降低血瘀模型大鼠血沉、红细胞聚集指数、血沉方程K值;10.0 g/kg组可明显降低血瘀模型大鼠全血黏度(切变率5、1/s),血沉、全血低切还原黏度、红细胞聚集指数、血沉方程K值、全血低切相对黏度.注射用丹红5.0、10.0 g/kg组可抑制血瘀大鼠血小板聚集和黏附,并可使体外血栓长度缩短、干湿重降低.结论 注射用丹红的抗缺血作用可能与改善血液流变学、抑制血栓形成、改善血小板黏附和聚集有关.     

注射用丹红对大鼠血液流变学及血栓、血小板功能的影响

目的观察注射用丹红对血液流变学及血栓、血小板功能的影响。方法 SD大鼠随机分为6组:对照组、血瘀模型组、注射用丹红(2.5、5.0、10.0 g/kg)组及丹参注射液组(5.0 g/kg)。制备大鼠急性血瘀模型,观察注射用丹红对大鼠血液流变学和血小板黏附和聚集的影响;采用体外血栓形成试验观察注射用丹红抗血栓形成作用。结果注射用丹红5.0 g/kg组可明显降低血瘀模型大鼠血沉、红细胞聚集指数、血沉方程K值;10.0 g/kg组可明显降低血瘀模型大鼠全血黏度(切变率5、1/s),血沉、全血低切还原黏度、红细胞聚集指数、血沉方程K值、全血低切相对黏度。注射用丹红5.0、10.0 g/kg组可抑制血瘀大鼠血小板聚集和黏附,并可使体外血栓长度缩短、干湿重降低。结论注射用丹红的抗缺血作用可能与改善血液流变学、抑制血栓形成、改善血小板黏附和聚集有关。     

复方生发Ⅰ号胶囊对老年大鼠血液流变的影响

目的：观察复方生发Ⅰ号胶囊对SD老年大鼠血液流变性的影响。方法：三个受试组分别用复方生发Ⅰ号胶囊2．1g／kg、1．05g／kg及0．74g／kg连续灌胃给药7天。分别测定给药前后大鼠血液高切变率下全血黏度（HS）、中切变率下全血黏度（MS）、低切变率下全血黏度（LS）、血浆黏度（PV）和红细胞压积（HCT）。观察红细胞聚集指数、变形指数、刚性指数、电泳指数、全血还原黏度（高、中、低切）。结果：复方生发Ⅰ号胶囊能明显降低SD老年大鼠各切变率下全血黏度及红细胞压积，降低全血还原黏度，降低SD老年大鼠的红细胞聚集指数，升高大鼠的红细胞变形指数。结论：复方生发Ⅰ号胶囊能明显改善SD老年大鼠的血液流变性。     

苗医验方“四大血”对急性血瘀模型大鼠血液流变学的影响

目的：观察“四大血”不同剂量提取物对急性血瘀大鼠血液流变学的影响。方法：采用大剂量注射肾上腺素加寒冷刺激的方法制备大鼠急性血瘀模型,给予不同剂量的“四大血”提取物,测定实验动物的血液流变学指标。结果：“四大血”的水提醇沉液的高（4g／kg）、中（2g／kg）、低（1g／kg）剂量组在切变率为1/s、5/s、50／s时可显著降低全血黏度;其高、低剂量组可显著降低全血还原黏度、红细胞聚集指数,而对红细胞压积和血浆黏度无明显影响。结论：“四大血”能够改善急性血瘀模型大鼠血液流变学的相关指标,具有一定活血化瘀作用。     

保肾颗粒对急性血瘀模型大鼠血液流变性的影响

目的:观察保肾颗粒对急性血瘀证模型大鼠血液流变性的影响。方法:SD大鼠60只随机分为空白对照组、模型对照组、复方丹参滴丸(阳性药)组和保肾颗粒3.6,1.8,0.9 g/kg剂量组,连续灌胃给药10 d后,采用皮下注射盐酸肾上腺素加冰水浴法制备大鼠急性血瘀证模型,于禁食20 h后腹主动脉采血,分别测定不同切变率下全血黏度、血浆黏度、全血还原黏度、红细胞压积等血液黏滞度指标。结果:与模型对照组比较,保肾颗粒3.6,1.8 g/kg剂量组能明显降低急性血瘀证大鼠的全血黏度、血浆黏度、红细胞压积和红细胞聚集指数(P0.05)。结论:保肾颗粒对急性血瘀证模型大鼠血液流变性具有一定的改善作用,能降低其血液黏度。     

桃红四物汤对产后血瘀证大鼠血清溶血磷脂酸的影响

目的探讨桃红四物汤对产后血瘀证大鼠血清溶血磷脂酸(lysophosphatidic acid,LPA)水平的影响。方法采用米非司酮(8.3mg/kg)和米索前列醇(0.1mg/kg)灌胃早孕鼠,复制血瘀证大鼠模型,将模型复制成功的大鼠随机分为模型组、益母草颗粒组(4.3g/kg)及桃红四物汤高(18.0g/kg)、中(9.0g/kg)、低(4.5g/kg)剂量组,并设立正常对照组;测定血液流变学指标以判定桃红四物汤治疗血瘀证的疗效,采用酶联免疫吸附试验测定血清LPA含量。结果模型组全血黏度、血浆黏度、血清LPA均较正常对照组显著增高(P0.05,或P0.01);桃红四物汤中剂量组及益母草颗粒组大鼠全血低切变率黏度和高切变率黏度显著降低(P0.05),各药物组大鼠血浆黏度显著降低(P0.05,或P0.01);桃红四物汤中剂量组及益母草颗粒组大鼠LPA水平显著降低(P0.05)。结论桃红四物汤祛瘀作用显著,其治疗作用可能与降低血清LPA的水平有关。     

佛香饮方对寒凝血瘀型痛经模型大鼠痛反应及血液流变学的影响

目的:探讨佛香饮方水提取物对寒凝血瘀型痛经模型大鼠的镇痛作用及其对血液流变性的影响,为其临床应用提供实验依据.方法:采用冰水浴、皮下注射盐酸肾上腺素,加ip注射缩宫素法建立大鼠寒凝血瘀型痛经模型.以扭体反应次数为指标,探讨佛香饮对痛反应的影响.以凝血时间(CT)、凝血酶原时间(PT)、凝血酶时间(TT)、活化部分凝血活酶时间(APTT)及血小板聚集率、红细胞压积(HCT)、血沉方程K值、血浆黏度、全血黏度为指标,探讨佛香饮对寒凝血瘀型痛经模型大鼠对凝血及血液流变学指标的影响.结果:佛香饮1.8g/kg,3.6g/kg和7.2g/kg剂量组大鼠的CT明显长于模型对照组(P<0.05或P<0.01),PT、TT及APTT均明显高于模型组(P<0.05或P<0.01).佛香饮3.6g/kg和7.2g/kg剂量组的血小板聚集率明显低于模型组(P<0.05或P<0.01),HCT、血沉方程K值均明显低于模型对照组(P<0.05);佛香饮各剂量组血浆黏度、全血黏度均低于模型对照组(P<0.05或P<0.01).结论:佛香饮对寒凝血瘀型痛经模型大鼠的镇痛和改善血液流变性作用是其治疗寒凝血瘀型痛经的主要作用基础.     

晶珠降脂排毒胶囊对“血瘀”大鼠血液流变学的影响

本文研究了晶珠降脂排毒胶囊对“血瘀”大鼠血液流变学的影响,结果显示该药ig给药（剂量０.5g/kg和０.２５g/kg）可明显降低“血瘀”大鼠的红细胞聚集指数以及低切变率下的全血粘度和全血还原粘度,剂量为０.5g/kg时,还可显著降低红细胞压积。提示该药对防止血栓形成和治疗血瘀症有一定作用。     

乌苏里藜芦碱在大鼠的血液流变学作用

[目的 ]研究乌苏里藜芦碱 (VnA)对大鼠血液粘度的影响。 [方法 ]正常和血淤大鼠ivVnA1 5 μg·kg- 1,30 μg·kg- 1和 4 5 μg·kg- 1后取血 ,以微机化血液比粘度仪测定全血比粘度、血浆比粘度和红细胞压积等血液流变学指标。 [结果 ]正常大鼠ivVnA后全血比粘度显著降低 ,低切变率时自 2 0 .87± 3.6 3(NS组 )降至 1 3.0 8± 2 .4 2 (VnA中剂量组 ,P <0 .0 1 )和 1 1 .89± 4 .77(VnA大剂量组 ,P <0 .0 1 ) ,高切变率时自 6 .1 4± 1 .0 5 (NS组 )降至 4 .71± 1 .0 3(VnA大剂量组 ,P <0 .0 5 )。血淤模型大鼠ivVnA同样导致全血比粘度的显著降低 ,在低切变率时中剂量组为 1 8.98± 3.36 ,大剂量组为 8.35± 3.34,而血淤模型组为 5 0 .81± 7.5 4,P <0 .0 1 ,在高切变率时 ,大剂量组为 8.32± 2 .74 ,而血淤模型组为 1 2 .5 7± 3.0 6 ,P <0 .0 5。VnA在大剂量时对血浆粘度也有一定降低作用(1 .4 6± 0 .0 3vs 1 .5 3± 0 .0 6 ,P <0 .0 5 ,正常大鼠 ;1 .5 7± 0 .1 2vs 1 .87± 0 .4 4,P <0 .0 1 ,血淤大鼠 )。对血细胞压积无显著影响。 [结论 ]静脉给予VnA对血粘度具有明显的降低作用 ,对低切变率全血粘度的降低尤为明显。其降粘作用不是红细胞数量和体积改变的结果     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.