鼻息肉中肥大细胞雌激素受体的表达及其意义

目的 检测雌激素受体在鼻息肉肥大细胞和ＣＤ４＾＋、ＣＤ８＾＋及ＣＤ６８＾＋细胞上的表达。方法 采用抗肥大细胞,抗ＣＤ４＾＋、ＣＤ８＾＋、ＣＤ６８＾＋细胞以及抗雌激素受体的单顾隆抗体对９例鼻息肉标本进行免疫组化及双标免疫染色。结果 所有上述细胞亚群中相应抗体清晰地检测到。形态学观察,雌激素受体阳性细胞明显与ＣＤ４＾＋、ＣＤ８＾＋、ＣＤ６８＾＋细胞不同,但也肥大细胞相似,双标免疫洒以,只有肥大细胞而非     

鼻息肉中肥大细胞雌激素受体的表达及其意义

目的检测雌激素受体在鼻息肉肥大细胞和ＣＤ４＋、ＣＤ８＋及ＣＤ６８＋细胞上的表达。方法采用抗肥大细胞,抗ＣＤ４＋、ＣＤ８＋、ＣＤ６８＋细胞以及抗雌激素受体的单克隆抗体对９例鼻息肉标本进行免疫组化及双标免疫染色。结果所有上述细胞亚群均可被相应抗体清晰地检测到。形态学观察,雌激素受体阳性细胞明显与ＣＤ４＋、ＣＤ８＋、ＣＤ６８＋细胞不同,但与肥大细胞相似;双标免疫染色,只有肥大细胞而非ＣＤ４＋、ＣＤ８＋、ＣＤ６８＋细胞与雌激素受体阳性细胞的位置相吻合。结论鼻息肉中肥大细胞表达雌激素受体。这一结果将对揭示呼吸道变态反应和炎性疾病的病理以及雌激素参与炎性反应性疾病的机制将有理论和实用价值。     

肥大细胞及其类胰蛋白酶在鼻息肉发病中的作用

摘要：目的对鼻息肉中的肥大细胞及其类胰蛋白酶（mast cell tryptase,MCT）进行定位和定量分析,探讨肥大细胞及其类胰蛋白酶在鼻息肉发病中的作用。方法选取符合纳入标准的鼻息肉患者22例作为实验组,采集手术切除标本22份;选取同时行下鼻甲部分切除术的鼻中隔矫正术住院患者12例作为对照组,采集下鼻甲黏膜组织标本12份。采用ELISA法检测鼻息肉中肥大细胞类胰蛋白酶、IL 8、嗜酸性粒细胞趋化因子（Eotaxins）的表达状况,并用免疫组化法对鼻息肉中的肥大细胞及其类胰蛋白酶进行定位和证实。结果鼻息肉组中MCT、IL 8、Eotaxins的蛋白表达高于下鼻甲组,且MCT与IL-8之间、MCT与Eotaxins之间存在中等正相关。免疫组化提示鼻息肉组中存在大量的肥大细胞,类胰蛋白酶的表达明显高于下鼻甲组。结论肥大细胞类胰蛋白酶在鼻息肉中IL 8、Eatoxins等的分泌、炎性细胞的募集以及鼻息肉中上皮增生、间质水肿、血管增生扩张过程中扮演了十分重要的角色。     

鼻息肉

本文扼要阐述了鼻息肉病因、发病机制和发病部位。一些新的实验资料支持、鼻息肉形成的上皮破裂理论。在鼻息肉形成的初期阶段：①上皮破裂伴固有层突出；②脱出部分的上皮化生；③鼻息肉进一步发展。关于鼻息肉起源的研究表明：鼻息肉主要是起源于筛房裂附近的中鼻道粘膜。［引言）近10年来，人们之所以对鼻息肉产生了极大的兴趣，主要由于对鼻息肉治疗的多样化，其中包括全身或局部皮质类固醇治疗；简单的采用囵套器切除息肉；鼻内窥镜或显微镜下筛窦部分的或完全的切除术。由于合理的治疗必须建立在充分了解疾病形成过程的基础上。重要的…     

219例慢性鼻窦炎、鼻息肉患者临床资料分析

结合一组临床病例资料的回顾性分析结果，探讨慢性鼻窦炎、鼻息肉发病、复发的影响因素，提出开展功能评价及围手术期综合治疗（特别是抗变态反应治疗）的必要性。     

鼻粘膜肥大细胞的分化与增殖免疫组织化学研究

鼻变态反应患者 13例 (年龄 14～ 5 9岁 ,平均 2 7.2岁 ) ,非鼻变态反应患者 5例 (年龄 31～ 6 0岁 ,平均4 4.6岁 )。于鼻内窥镜下鼻窦手术等时 ,采取下鼻甲粘膜、固定、切片 ,分别行抗类胰朊酶抗体、抗 C- Kit抗体、抗增殖细胞核抗原 (PCNA)抗体、抗凝乳酶肥大细胞抗体免疫染色。然后于 4 0 0倍镜下 ,随意观察 5处上皮层、粘膜固有浅层、深层各抗体阳性细胞数目 ,两组间对比。结果发现 ,类胰朊酶阳性细胞数目 ,鼻变态反应组上皮层和粘膜固有层均较非鼻变态反应组多 (P<0 .0 5和 P <0 .0 1)。C- Kit和 PCNA阳性细胞数 ,鼻变态反应组上…     

嗜酸粒细胞型鼻息肉患者血清特异性IgE检测的临床分析

目的:探讨不同类型鼻息肉(NP)患者对吸入性变应原的超敏性及相关性。方法:将83例NP患者按息肉标本内嗜酸粒细胞(EOS)计数多少分为嗜酸粒细胞型鼻息肉(ENP)和非嗜酸粒细胞型鼻息肉(nENP),所有NP患者进行血清变应原特异性IgE(sIgE)检测及统计学分析。结果:ENP和nENP患者分别为27例(32.53%)、56例(67.47%),其血清变应原sIgE阳性者分别为16例(59.26%)、13例(23.21%),2种类型间比较差异有统计学意义(P<0.01);尘螨是NP组最常见的变应原。结论:常年性吸入性变应原与ENP患者有相关性。     

人鼻息肉黏膜上皮细胞3种原代体外培养方案的比较

目的:比较人鼻息肉黏膜上皮细胞3种原代体外培养方法的效果,探讨适宜的体外培养方案。方法:对人鼻息肉黏膜上皮细胞分别以酶消化分离法、酶消化分离细胞ABC法和组织块培养法进行原代体外培养,观察细胞形态,掌握其生物学特性,并对3种方案的培养成功率和细胞生长曲线进行比较。结果:酶消化分离细胞ABC法体外培养成功率为87.5%,优于酶消化分离法(78.13%)和组织块培养法(84.38%),但三者间差异无统计学意义(P>0.05)。酶消化分离法和酶消化分离细胞ABC法的细胞生长曲线无明显差异,均高于组织块培养法。结论:酶消化分离细胞ABC法细胞增殖迅速,无其他细胞混杂,可建立稳定、可靠的人鼻黏膜上皮细胞原代培养模型,可为鼻息肉的相关研究提供良好的细胞系。     

白介素-2信号通路在鼻息肉发病中的作用

目的：研究白介素2（IL-2）信号通路相关因子及调节性T细胞在鼻息肉患者组织中的表达情况,探讨二者之间的关联及其在息肉发病中的可能作用机制。方法：集取鼻息肉及正常对照组共30例患者的息肉组织及鼻甲黏膜,采用ELISA法检测局部组织内IL-2及IL-2R,免疫印迹检测组织中的磷酸化STAT5,实时荧光定量PCR法检测Foxp3mRNA在局部组织的表达情况;采用流式细胞术检测局部组织内Treg细胞的比率;同时分析IL-2通路相关因子及Treg细胞的相关性。结果：鼻息肉患者局部组织中IL-2、IL-2R及pSTAT5的含量较正常对照组显著降低,差异有统计学意义（均P〈O．05）;同时,与正常对照组相比,鼻息肉患者的Foxp3mRNA及Treg细胞的比率显著下调,差异有统计学意义（均P〈O．05）;且鼻息肉局部组织内IL-2信号通路相关因子的表达与Treg细胞比率及Foxp3mRNA水平呈正相关（均P〈O．01）。结论：IL-2信号通路的活化状态在鼻息肉患者局部组织中发生了变化,且与Treg细胞的表达呈正相关,提示IL-2信号通路在鼻息肉的发病中起重要作用,鼻息肉中Treg细胞的下调可能是由IL-2信号通路的活化下调所引起的。     

Histopathological examinations of nasal polyps of different etiology

Summary A safe differentiation between allergic and non-allergic polyps appears impossible by means of light or electron microscopical examination of polyp tissue. There are some indications, however, which point to the allergic or non-allergic origin and it seems that the relationship between eosinophils and plasma cells with a low index is characteristic for polyps of allergic origin.Eosinophils and round-cell infiltrations are present in all types of polyps. An index of eosinophils to plasma cells below 5 is indicative for allergy and should induce the pathologist to give a hint to the clinician for allergological work-up. An index above 5 is more corresponding with infective etiology.Any changes of the basement membrane, i.e., smaller or larger gaps or even absence, are found only very irregularly and are not typical findings in either way.An increased number of glands and collagenic fibres, especially under the epithelium, are more an expression of the age of the polyp.Since the conservative therapy as mentioned above leads to good therapeutical results, it is desirable that an allergological work-up should be done in any case which shows the histological findings indicative for allergic etiology. Thus, repeated operations with all the risks and uncertain therapeutical benefit can be avoided.     

复发性鼻息肉的鼻内窥镜修正术

目的：探讨鼻内窥镜修正术治疗复发性鼻息肉的疗效和手术体会。方法：回顾分析１９９７年７月－１９９８年１０月间２１例因鼻息肉复发在我院行鼻内窥镜修正术的患者,２１例均双侧同时手术,根据ＣＴ所见及术中情况,采用筛窦全切或次全切,上颌窦自然窦口扩大,尖牙窝径路上颌窦病变组织清除等手术方式。结果：随访６－１２个月评定疗效,治愈７傅,好转８例,无效６例,总有效率７１．４％,无一例出现严重并发症。结论：鼻内窥镜     

Apoptosis in eosinophilic nasal polyps treated in vitro with Mitomycin C

The etiopathogenesis of eosinophilic nasal polyps is yet to be explained. Eosinophils are key components in the inflammatory infiltrate and are related to the perpetuation of the inflammatory process in chronic rhinosinusitis with nasal polyps.ObjectiveThis paper aims to evaluate the in vitro action of mitomycin upon the apoptotic index of nasal polyps.Materials and MethodsThis is a self-paired prospective experimental study using biopsy fragments from 15 patients with eosinophilic nasal polyps. Biopsy fragments were divided into two groups. In the case group, the fragments were treated with 400 µg/ml of mitomycin for five minutes. The control group fragments were treated with culture medium. The pair of fragments contained in the two first compartments - control and case - were immediately sent to the histopathologist. The other pair of samples containing control and case fragments was incubated for 12 hours. The fragments were then taken to the histopathologist for testing. The apoptotic index was determined by the morphometry in hematoxylin and eosin staining and DNA fragmentation analysis (TUNEL reaction).ResultsThe comparison between the two groups showed a statistically significant difference (p < 0,001) in the apoptotic index of the 12-hour incubated cultures.ConclusionMitomycin acts in vitro upon the eosinophilic nasal polyps inducing the rise of the eosinophilic apoptotic index.     

Paraganglioma of the skull base presenting as nasal polyps

The authors report a case of paraganglioma of the skull base presenting as nasal polyps. A 29‐year‐old patient presented with epistaxis and was found to have nasal polyps. The patient underwent a nasal polypectomy. After pathology showed an unusual appearance, the mass was subsequently excised endoscopically using radiofrequency coblation, and it was found to be originating from the skull base. The diagnosis was made using a combination of clinical findings, radiology, and histopathology examination. It is important to consider paraganglioma in the differential diagnosis of unusual tumors of the nose and skull base. Laryngoscope, 2013     

Role of blood inflammatory cells in chronic rhinosinusitis with nasal polyps

Background/Objective: The pathophysiological and prognostic role of blood inflammatory cells in chronic rhinosinusitis with nasal polyps (CRSwNP) emerging from recent studies was investigated.

Material and Methods: The main available evidence and largely-recent publications were critically analyzed.

Results: Several authors reported a direct association between blood eosinophilia and CRSwNP recurrence rates. In some large series, a direct association between recurrent CRSwNP and blood basophil values emerged too. CRSwNP patients’ blood eosinophil and basophil values were strongly related. It was also found that preoperative neutrophil-to-lymphocyte, eosinophil-to-lymphocyte, and basophil-to-lymphocyte ratios were significantly higher in patients who experienced a disease relapse than in those who did not. In histologically-confirmed eosinophilic-type CRSwNP treated with endoscopic sinus surgery, mean blood eosinophil values dropped significantly from before to after the surgical procedure.

Conclusions/Significance: CRSwNP endotypes have different inflammatory profiles reflected in the relative proportions of different types of blood cells. The available data support the theory that blood eosinophil and basophil levels should be included in the routine preoperative work-up of CRSwNP patients in order to give patients accurate prognostic information, adopt rational follow-up protocols after surgery, and provide dedicated postoperative medical treatments.