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1.
C L Weill 《Brain research》1986,391(2):305-309
The concentration of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) was measured in developing chick ciliary (CG) and dorsal root ganglia (DRG) as a function of embryonic age from day 8 through day 18 using radioimmunoassays. The concentration of cAMP and cGMP increased in both ganglia from day 8 through day 14. cAMP levels were nearly two-fold higher in DRG than in CG. Normalization of the data for ciliary ganglia to the number of cells per ganglion and calculation of their molar concentrations indicates a 48% increase in cGMP and a 3.2-fold increase in cAMP during the developmental process of natural neuronal cell death. 相似文献
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Development of the chick dorsal root ganglia was examined in 4.5- to 9.5-day embryos. Tritiated thymidine (3H - TdR) and autoradiography was used to analyze proliferative activity and the Feulgen procedure to analyze degenerative activity in ganglia 12–17. Proliferative activity was found to be elevated through 4.5 days of incubation when as many as 14% of the ganglionic cells become labelled following a one-hour exposure to 3H- TdR. By 6.5 to 7.5 days proliferative activity decreased to 2–4% in the lateroventral (LV) regions and to approximately 1% in the mediodorsal (MD) regions of the ganglia. However there appears to be increased proliferative activity by the end of the experimental period at 9.5 days. Birthdate studies demonstrate that large-scale neuronal production occurs between 4.5 and 6.5 days in LV regions and between 4.5 and 7.5 days in the MD regions. After those times ganglionic proliferative activity must be largely nonneuronal in nature. This nonneuronal proliferation is greater in LV than in MD regions and in brachial than in nonbrachial ganglia. Degenerative activity was found to be absent from the ganglia until after 4.5 days of incubation. It then increases rapidly, and by 5.5 days 5% of the LV cells in nonbrachial ganglia are degenerating. Degenerative activity then declines but is still present at 9.5 days. In contrast to results of an earlier study (Hamburger and Levi-Montalcini, '49), degenerative activity was also found in the LV region of brachial ganglia and the MD regions of brachial and nonbrachial ganglia. The pattern of LV degenerative activity in brachial ganglia is similar to that in nonbrachial activity increases throughout the experimental period, and by 9.5 days as many as 4% of the MD cells are degenerating. 相似文献
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Estrogen receptors (ER) are widely distributed in the central nervous system (CNS). Recent studies, to date in rat only, have shown that ER are also expressed in neurons of the dorsal root ganglia (DRG) where they appear to have functional roles. However, no data yet exists about estrogen receptors in the embryonic DRG. In the present study, immunocytochemical staining for ER in the DRG of chick embryos from day 6.5 to 18.5 (Hamburger and Hamilton St. 30–45) of incubation was performed. ER+ cells were first consistently observed at day 8.5 (St. 34), more concentrated in the ventral-lateral portion of the DRG. From day 8.5 to 12.5 (St. 38), the density of ER+ cells and the staining intensity increased, with no obvious changes from day (E) 12.5 to 18.5. Although ER is detected mainly in the cytoplasm of embryonic DRG neurones, ER+ cells with nuclear staining are sometimes observed and gradually increase in number during development. ER-immunoreactivity in the DRG at cervical, thoracic and lumbo-sacral levels is similar and no obvious differences in staining were observed between male and female embryos. ER+ neurons are also present in the sympathetic ganglia from E8.5 and some primary spinal motoneurons are ER+ beginning at E14.5. The results suggest that estrogen may play a role in the embryonic development of the DRG. 相似文献
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We screened a variety of lectins with different sugar specificates to determine whether subpopulations of dorsal root ganglion (DRG) neurons in the chick can be distinguished by the carbohydrates they express. Of the 15 lectins tested only those that recognize N-acetylgalactosamine (galNac) residues labeled a subset of DRG neurons. For example, Dolichos biflorus (DBA) labeled a population of small-diameter neurons in the dorsomedial DRG and their terminals in the dorsal horn in hatchling chicks. Staining of live neurons in vitro demonstrated that DBA was binding to the cell surface. Labeling first appeared in sensory neurons at about St.38 (E12) and in dorsal horn laminae 1 and 2 at about St.42 (E16). Fainter labeling appeared somewhat later in lamina 3, after hatching. Labeling of the tissue sections was eliminated by chloroform: methanol extraction and reduced by alpha-N-acetylgalactosaminidase digestion, but survived trypsinization. Together these results suggest that a subset of DRG neurons in the chick can be identified by the presence of a cell surface glycoconjugate, perhaps a glycolipid, containing terminal alpha-linked galNac residues. 相似文献
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We examined the distribution of carbonic anhydrase in dorsal root ganglia and spinal cords of chick embryos from Stage 26 (5 days) through hatching using the Hansson technique (Hansson, H.P., Histochemistry, 11 (1967) 112-128; Lannerholm, G., Ann. N.Y. Acad. Sci., 429 (1984) 369-381). Although reactivity was apparent in this tissue at all stages (e.g. in endothelial cells, erythrocytes, and cell nuclei) carbonic anhydrase-positive dorsal root ganglion neurons were not detected until Stage 35 (8.5-9 days). At this stage fewer than 1% of neurons were reactive. The number and proportion of carbonic anhydrase-positive dorsal root ganglion neurons increased steadily from Stage 35 to hatching, when about 60% of the neurons were reactive. In hatchlings carbonic anhydrase-positive neurons spanned the entire size spectrum of ganglion cells and appeared to be randomly distributed throughout the dorsal root ganglion. No carbonic anhydrase-positive neurons were found in the spinal cord at any stage. However, in hatchlings the background staining was markedly lighter in laminae 1 and 2 and in Lissauer's tract than in the rest of the cord, suggesting that unmyelinated axons in the CNS are not reactive. 相似文献
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Giordano D Giorgi M Tata AM Modica A Augusti-Tocco G 《Journal of neuroscience research》2004,78(6):815-823
Cyclic GMP (cGMP)-binding cGMP-specific phosphodiesterase (PDE5) activity was found in chick dorsal root ganglia (DRG). PDE5 expression was studied at different stages of development: in embryonic day 10 (E10) and E18 embryos and in 5-day post-hatching chick (P5). The presence of PDE5 was suggested by the ion exchange chromatography elution profile in E18 DRG extracts, where cGMP-specific hydrolytic calmodulin-independent activity was found; in other stages, this activity coeluted with the PDE1 calmodulin-stimulated isoform characterized previously. Inhibition studies supported the hypothesis that the newly identified PDE activity belongs to the PDE5 isoform. Western blot analysis using a PDE5-specific antibody was also carried out and revealed the presence of three specific immunoreactive bands with apparent molecular weights of 98, 93, and 86 kDa, corresponding to the three described splice variants (PDE5A1, PDE5A2, and PDE5A3). The expression in DRG of the three PDE5 isoforms was also confirmed by RT-PCR. Developmental regulation of PDE5 was revealed by the immunoblot analysis at different stages; expression was very low at E10 but an overall substantial increase occurred between E10-18 (about 12-fold, considering the three PDE5 isoforms together). Differences were revealed, however, when a single PDE5 isoform was considered. PDE5A1 and PDE5A3 showed an increase at all stages although more pronounced between E10-18, whereas PDE5A2 underwent a marked increase (about 38-fold) in the first period and remained nearly constant between E18 and P5. This is the first evidence of PDE5 in sensory neurons, and the distinct temporal expression patterns of enzyme isoforms may indicate different physiologic roles in developing and mature chick DRG. 相似文献
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A. M. Tata M. Plateroti M. Cibati S. Biagioni G. Augusti-Tocco 《Journal of neuroscience research》1994,37(2):247-255
The presence of acetylcholinesterase has been reported in chick dorsal root ganglia at early developmental stages although acetylcholine is not known to play a role in these ganglia. Recently, we reported that during development the level of acetylcholinesterase increases continuously and the enzyme becomes gradually expressed in all sensory neurons. These observations prompted the study of the developmental pattern of expression of other cholinergic markers, such as choline acetyltransferase (ChAT) and the high affinity transport mechanism for choline. ChAT activity is barely detectable at early developmental stages (E7) and increases markedly thereafter, with an activity profile similar to that described for acetylcholinesterase. A similar increase in enzyme activity is also observed when ChAT is measured in dorsal root ganglia explants and in dissociated cells in culture. The study of ChAT activity in cultured cells shows an increase over a period of 3 days, thus ruling out the hypothesis that motor fibers, still associated to the ganglia, may represent a possible source of the enzyme. Immunostaining of whole ganglia or cultured cells shows that ChAT immunoreactivity is not restricted to a specific neuronal subpopulation but appears as a common marker of sensory neurons. High affinity choline uptake, blocked by hemicholinium, is present in sensory neurons cultured from E7 dorsal root ganglia. Observations on cultured neurons from later stages (E18) indicate that choline transport is not a transient property of sensory neurons. These observations show a similar pattern of expression of several cholinergic markers during development. Such a pattern is maintained at significant levels also in mature ganglia. © 1994 Wiley-Liss, Inc. 相似文献
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Early development of the dorsal root ganglia and ventral roots in quail embryos was observed by using HNK-1 antibodies. The dorsal root ganglia showed a dumbbell form in the horizontal plane, i.e. one dorsal root ganglion was constituted by two groups arranged craniocaudally. These observations suggest that the complex form of the dorsal root ganglia is brought about by a subsegmentation of the neutral tube and peripheral nerve supply. Further, it was observed that the segmental boundaries of the neural tube, which were indicated by the individual ventral roots, differed from the boundaries indicated by mesodermal segmentation. These findings seem to provide us with a new viewpoint for analyzing the nervous system. 相似文献
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Effects of male and female sex steroids on the development of normal and the transient Froriep's dorsal root ganglia of the chick embryo 总被引:2,自引:0,他引:2
Sex steroids can influence developmental processes and support the survival of neurons in the embryonic central nervous system. Recent studies have shown that estrogen receptors are also expressed in the peripheral nervous system, in the dorsal root ganglia (DRG) of chick embryos. However, no studies have examined the effects of sex steroids on development of embryonic DRG. In the present study, 0.2 microg, 1.0 microg, 5.0 microg 10 microg, 20 microg, 25 microg, and 40 microg doses of testosterone or estradiol were delivered to chick embryos at Hamburger and Hamilton stage 18 (E3). The actions of these doses of sex steroids on the development of the C5DRG (fifth cervical ganglion, a "normal" DRG) and C2DRG (a transient ganglion known as a "Froriep's DRG") were then evaluated by quantifying ganglionic volumes, cell number, proliferation, and apoptosis after 1 day of growth to stage 23. We found that both testosterone and estradiol promoted proliferation of cells in both normal DRG and the Froriep's ganglia. By contrast, estradiol significantly increased the number of apoptotic cells, while testosterone strongly inhibited apoptosis. These actions of sex steroids on DRG development were dose-dependent, and C5DRG and C2DRG showed different sensitivities to the applied sex steroids. In addition, the present results demonstrated that specific ER and AR inhibitors (tamoxifen and flutamide) did not influence the effects of 5 microg E2 and 5 microg T on C2 and C5DRG significantly. These results demonstrate that male and female sex steroids can modulate DRG development through an epigenetic mechanism, as had been shown for the central nervous system. 相似文献
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The aim of this study was to investigate the influence of collagen or polyornithine substrates on cell migration in explant cultures of dorsal root ganglia (DRG) by means of light microscopy and immunocytochemistry. Myelin-associated glycoprotein (MAG) immunoreactivity was used to characterize the subpopulation of small B ganglion cells, whereas neuron-specific enolase (NSE) immunoreactivity acted as a general neuronal cell marker. After a few days in culture, DRG explants grown on collagen substrate showed a flattened shape consisting of a core surrounded by a crown of neurites, which were mixed up with migrating cells of different types. These migrating cells were immunostained for both MAG and NSE and were observed after 7 days in the vicinity of the explant core, then after 14 days also at a distance from the explant core. In contrast, even after 14 days in culture, explants grown on polyornithine substrate maintained a globular shape. The MAG-positive ganglion cells were confined to the explant core and no cell migration was observed on this type of substrate. MAG immunoprecipitates located at the ganglion cell surface were observed in explants cultured on polyornithine, but rarely on collagen substrate. In conclusion, it is suggested that this pattern of intracellular distribution of MAG immunoreactive material could reflect interactions between cell surface and extracellular matrix, and could condition the migratory ability of small ganglion cells. 相似文献
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Meijer C de Vries EG Marmiroli P Tredici G Frattola L Cavaletti G 《Neurotoxicology》1999,20(6):883-887
The mechanism(s) and site(s) of the neurotoxic effect of cisplatin (CDDP) are still not entirely elucidated. A more detailed knowledge of these aspects of CDDP treatment might be useful to obtain a better understanding of the pathogenesis of its peripheral neurotoxicity, which is the dose-limiting side effect of CDDP. In the present study, the occurrence of CDDP-induced DNA-platination in dorsal root ganglia (DRG) of rats was evaluated in relation to DRG neuron pathological changes and CDDP-induced neuronopathy. Eight adult Wistar rats were treated with 2 mg/kg i.p. CDDP twice weekly for 9 times to induce sensory peripheral neuropathy. DNA-platination in specimens of DRG and kidney was measured immunohistochemically, with a polyclonal antibody (GPt) detecting CDDP-induced Pt-DNA adducts. Results were compared with those of untreated rats. Chronic CDDP-induced neurotoxicity, in a well described experimental model of chronic CDDP neurotoxicity in the Wistar rat, was confirmed by sensory DRG neuronopathy with secondary neuropathy, and demonstrated by reduced pain detection, decreased nerve conduction velocity in the tail nerve as well as morphological and morphometric changes in DRG neurons. Nuclear immunostaining for Pt-DNA adducts was observed in tubular cells of the kidney in 75% of the evaluated CDDP-treated rats, while in DRG cells CDDP-induced Pt-DNA adducts formation was found in 43% of the evaluated CDDP-treated rats. CDDP-induced DNA-platination was demonstrated in rat DRG neurons using a schedule of chronic CDDP administration which induced the onset of a sensory neuronopathy with secondary peripheral neuropathy. This finding further supports the hypothesis that CDDP is neurotoxic because it directly damages the DRG neurons. 相似文献
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Neurogenesis in postnatal mouse dorsal root ganglia. 总被引:5,自引:0,他引:5
Neurogenesis continues in various regions of the central nervous system (CNS) throughout life. As the mitogen basic fibroblast growth factor (bFGF) can proliferate neuronal precursors of CNS neurons in culture, and is also upregulated within adult dorsal root ganglia following axotomy, it is possible that the postnatal dorsal root ganglia contain bFGF-responsive neuronal precursors. We undertook cell culture of postnatal mouse dorsal root ganglia to demonstrate neurogenesis. Basic FGF induced a cellular proliferative response in dorsal root ganglia cell culture. After 2 weeks in serum-free medium containing bFGF, neurons were rarely observed. However, following removal of bFGF and addition of trophic factors, many cells were observed that morphologically resembled dorsal root ganglia neurons, stained for neuronal markers, and generated action potentials. Furthermore, bromodeoxyuridine, used as a marker of cytogenesis, was detected in neurofilament-160(+) and/or microtubule-associated protein-2(+) cells that morphologically resembled neurons. In addition to bFGF, epidermal growth factor, nerve growth factor, and sonic hedgehog were also capable of generating spherical cell clusters that contained cells that stained for neuronal markers following the addition of trophic factors. These results suggest that early postnatal dorsal root ganglia contain neural precursors that appear to proliferate in response to various factors and can then be induced to differentiate into neurons. In conclusion, the existence of neural precursors and the possibility of neurogenesis in postnatal dorsal root ganglia may provide a greater range of plasticity available to somatosensory systems during growth or following injury, perhaps to replace ineffectual or dying neurons. 相似文献
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Taiseung Kim Ben H. Choi W. Choe R. C. Kim W. Van Nostrand S. Wagner D. Cunningham 《Molecular and chemical neuropathology / sponsored by the International Society for Neurochemistry and the World Federation of Neurology and research groups on neurochemistry and cerebrospinal fluid》1992,16(3):225-239
Protease nexin-II (PN-II) is a potent chymotrypsin inhibitor that forms SDS-stable inhibitory complexes with epidermal growth factor binding protein, the γ-subunit of nerve growth factor, and trypsin, and represents the secreted form of the amyloid β-protein precursor (APP) that contains the Kunitz-type protease inhibitor domain. To determine the expression of PN-II within the peripheral nervous system, human dorsal root ganglia were processed for immunocytochemistry using well-characterized monoclonal antibodies against PN-II and forin situ hybridization studies using35S-RNA PN-II probes for both APP751 and APP770. Highly specific immunoperoxidase staining of PN-II was demonstrated within the cytoplasm of dorsal root ganglia neurons and their processes in cryostat (fresh frozen) and vibratome (paraformaldehyde-fixed) sections.In situ hybridization using an anti-sense35S-RNA PN-II probe demonstrated the presence of intense neuronal labeling. Labeling was not observed when the corresponding sense35S-RNA PN-II probe was used. Although the precise functional role of PN-II/APP is not clear, the accumulation of amyloid β-protein within the neuropil appears to be one of the earliest events in the pathogenesis of Alzheimer’s disease (AD). Thus knowledge of the cell populations expressing the PN-II/APP gene would certainly be helpful for studies of the molecular mechanisms leading to the morphological and functional changes of AD. The results of this study clearly establish the expression of PN-II and its mRNA within the dorsal root ganglia neurons and their processes, and provide another point of departure for studies of the molecular mechanisms underlying the deposition of amyloid β-protein and its relationships to the formation of neuritic plaques and neurofibrillary tangles. 相似文献
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Neurotrophic activity of S-100 beta in cultures of dorsal root ganglia from embryonic chick and fetal rat 总被引:9,自引:0,他引:9
L J Van Eldik B Christie-Pope L M Bolin E M Shooter W O Whetsell 《Brain research》1991,542(2):280-285
We report here that S-100 beta, a protein with neurotrophic activity on central nervous system neurons, stimulates neuritic outgrowth from cultures of dorsal root ganglia (DRG). S-100 beta elicited neurites from explant and dissociated cell cultures of embryonic chick DRG, and the extent of the response varied with the age of the embryo. Specificity was demonstrated by the observation that incubation of S-100 beta with antibodies directed against S-100 beta reduced the neurite outgrowth, whereas incubation of S-100 beta with normal rabbit serum had little effect. S-100 beta also stimulated the area of neuritic outgrowth from organotypic cultures of fetal rat DRG, showing that the activity of the protein is not restricted to a particular species or culture condition. A mutant S-100 beta lacking neurotrophic activity on cerebral cortex neurons was unable to effectively stimulate neurite outgrowth from DRG cultures. These studies suggest that S-100 beta may play a role in neuronal growth and/or maintenance in the peripheral nervous system. 相似文献