血清CA125、 CA19-9、 MMP-9及血浆D-dimer联合检测在卵巢癌早期诊断中的价值研究

目的 探讨血清糖类抗原125(CA125)、糖类抗原199 (CA19-9)、基质金属蛋白酶-9(MMP-9)及血浆D-二聚体(D-dimer)联合检测在卵巢癌早期诊断中价值.方法 选取我院于2013年3月至2017年3月间收治的70例卵巢癌患者作为观察组,并选取同一时期我院收治的50例良性卵巢肿瘤患者作为疾病对照组,以及50例体检健康者作为健康对照组.采用电化学发光法(ECLIA)检测三组的血清CA125、CA19-9水平,采用酶联免疫法(ELISA)检测三组的血清MMP-9及血浆D-dimer水平,并对比3组上述4指标的检测结果.结果 观察组患者血清CA125、CA19-9、MMP-9及血浆D-dimer水平均显著低于两对照组,差异有统计学意义(P＜0.05);且不同临床分期患者的血清CA125、CA19-9、MMP-9及血浆D-dimer水平比较差异有统计学意义(P＜0.05);观察组患者CA125、CA19-9、MMP-9及D-dimer检测的阳性率均明显高于两对照组(P＜0.05).此外,血清CA125、CA19-9、MMP-9及血浆D-dimer四者联合检测的灵敏度相比于各指标单独检测均显著升高(P＜0.05);而联合检测的特异性相比于单独检测均有所降低,且相比于MMP-9、D-dimer差异有统计学意义(P＜0.05);同时联合检测的准确度相比于单独检测均有所升高,但差异无统计学意义(P＞0.05).结论 血清CA125、CA19-9、MMP-9及血浆D-dimer用于卵巢癌的早期诊断,体现一定的临床价值,而4种标志物联合检测显著提高了诊断的灵敏度及特异性,具有更大的临床应用价值.     

血清人附睾蛋白4和糖类抗原125检测在早期卵巢癌诊断中的应用

目的:检测分析肿瘤标志物人附睾蛋白4(HE4)和糖类抗原125(CA125)在早期卵巢癌诊断中的应用价值。方法:临床病检确诊早期卵巢癌患者32例为卵巢癌组,女性良性肿块患者79例为良性肿块组,另选30例健康女性作为健康对照组。术前采血,应用电化学发光法平行检测各组血清HE4和CA125水平,分析HE4、CA125单独和联合检测对卵巢癌的诊断效能。结果:卵巢癌组HE4和CA125水平均显著高于良性肿块组和健康对照组(P均0.01);HE4和CA125诊断卵巢癌的受试者工作特征曲线下面积(AUC)分别为0.935和0.896,差异无统计学意义(P0.05);HE4、CA125单独和联合检测诊断卵巢癌的敏感度分别为84.38%、87.50%和81.25%,差异无统计学意义(P0.05),HE4单独和HE4、CA125两者联合检测的特异性分别为96.20%和98.73%,均显著高于CA125单独检测(P0.01)。结论:HE4是特异性较高的卵巢癌血清标志物,其与CA125联用可以提高对早期卵巢癌的诊断准确性。     

HE4和CA125联合检测对卵巢癌的诊断价值

探讨人附睾上皮分泌蛋白4(HE4)和糖链抗原125(CA125)联合检测对卵巢癌的诊断价值。方法分别检测卵巢癌(n=37例)和妇科良性疾病(n=38例)患者血清HE4和CA125的表达水平,同时选择40例门诊体检妇女作为对照组。结果卵巢癌组血清HE4、CAl25表达水平明显高于良性疾病组和对照组(<0.05),良性疾病组血清CAl25表达水平明显高于对照组(< 0.05);联合检测血清HE4、CA125表达水平的敏感性均高于两种指标单独检测。结论HE4和CA125联合检测可明显提高卵巢癌诊断的敏感性,值得临床推广应用。     

HE4、AFP、CA125和CEA在卵巢癌中的诊断意义

目的：探讨人附睾蛋白4（HE4）、甲胎蛋白（AFP）、糖类抗原125（CA125）和癌胚抗原在卵巢癌（CEA）中的联合诊断作用和意义。方法：选取2015年7月~2017年7月本院收治的100 例卵巢癌患者为实验组,选取同期健康体检女性患者50例为对照组。收集患者血清,并检测血清中HE4、AFP、CA125 和CEA水平变化进行统计分析。结果：卵巢癌血清中HE4、AFP、CA125和CEA水平显著高于对照组和良性病变组患者,差异具有统计学意义（P<0.05）。且卵巢癌患者血清中HE4、AFP、CA125 和CEA联合检出率、灵敏度和特异度显著高于各指标的单独检出率,差异具有统计学意义（P<0.05）。结论：卵巢癌患者血清中HE4、AFP、CA125 和CEA含量呈过度表达状态;HE4、AFP、CA125 和CEA的联合检测在卵巢癌的诊断中具有重要的临床意义。     

血清HE4、CA125和CA724检测在卵巢恶性肿瘤诊断中的应用

目的通过检测卵巢恶性肿瘤患者血清人附睾蛋白4(HE4)、CA125和CA724水平,探讨这三项检测在卵巢恶性肿瘤诊断中的临床价值。方法采用电化学发光免疫法(ECL)检测CA125和CA724水平,以ELISA法检测HE4水平。测定36例卵巢恶性肿瘤组患者、59例卵巢良性疾病患者及70名健康体检人群血清三项水平并进行比较。结果卵巢恶性肿瘤组患者血清HE4、CA125和CA724水平显著高于良性疾病组和健康体检组(P0.05),其中良性疾病组CA125和CA724水平高于健康体检组(P0.05),但是良性疾病组HE4水平与健康体检组比较差异无统计学意义(P0.05);卵巢恶性肿瘤组患者血清HE4、CA125、CA724单独检测的灵敏度分别为69.4%、83.3%、58.3%,而这三项联合检测的灵敏度为91.7%,明显高于三项单独检测(P0.05)。当以卵巢良性疾病组作为参照人群时,HE4、CA125、CA724单独检测的特异性分别为96.6%、62.7%、81.4%,而这三项联合检测的特异性为61.0%。结论 HE4在诊断卵巢恶性肿瘤上具有高度特异性的肿瘤标志物,区分良性或恶性肿瘤时应选择HE4检测;卵巢肿瘤疾病的筛查是可选择HE4、CA125、CA724和三项联合检测以提高诊断的敏感性。     

HE4与CA125联合检测在女性卵巢癌诊断中的应用

目的：探讨血清HE4与CA125联合检测在女性卵巢癌诊断中的应用价值,为卵巢癌的诊断提供参考。方法选取我院2012年7月~2014年7月收治的75例疑似卵巢癌患者作为研究对象,根据病理检查结果将患者分为良性病变组25例、卵巢癌组50例,对两组患者血清HE4及CA125水平进行测定,分析两组患者血清HE4水平的差异及与CA125联合检测的灵敏度。结果卵巢癌组患者血清HE4及CA125水平显著高于良性病变组(<0.05),在50例卵巢癌患者中,血清HE4及CA125联合诊断的敏感性为96%,显著优于血清HE4单项检测的40%,及CA125单项检测的50%。结论随着学界对HE4的深入研究,其对于卵巢癌发病风险的预测及预后判断均可起到重要的临床作用,HE4联合CA125检测灵敏性及特异性较高,有助于卵巢癌的早期发现。     

175例卵巢肿瘤患者血清肿瘤标志物水平分析

目的:分析卵巢肿瘤患者血清肿瘤标志物甲胎蛋白(AFP)、癌胚抗原(CEA)、糖链抗原125(CA125)、糖链抗原199(CA199)和附睾蛋白4(HE4)的水平及临床价值。方法:选取武汉大学人民医院2015-01—2018-10收治175例卵巢肿瘤患者的临床资料,依据术后组织病理报告结果,将患者分为卵巢癌组(n=88)和卵巢良性肿瘤组(n=87)。选取同期本院体检中心体检的健康女性41例为对照组。检测各组血清AFP、CA125、CA199、CEA和HE4水平并比较各组各指标差异。结果:与对照组和卵巢良性肿瘤组相比,卵巢癌组患者血清AFP、CEA、CA125、CA199和HE4水平均升高(P0.05或P0.01)。与对照组相比,卵巢良性肿瘤组血清CA125和CA199水平升高(P0.05或P0.01),AFP、CEA及HE4水平差异无统计学意义(P0.05)。相关性分析结果显示卵巢癌患者CA125与HE4水平呈正相关关系(r=0.237,P0.05)。结论:卵巢癌患者血清AFP、CEA、CA125、CA199和HE4水平均升高,且CA125与HE4水平呈明显正相关,二者联合检测或可提高卵巢癌检出率。     

血清HE4和CA125联合检测在卵巢癌诊断中的应用分析

目的探讨血清人附睾蛋白4(HE4)和糖类抗原125(CA125)水平联合检测在卵巢癌诊断中的应用价值。方法对85例卵巢癌组患者、55例盆腔良性疾病组患者和120名对照组用ELISA测定血清HE4水平,用ECLIA测定CA125水平。结果卵巢癌组血清HE4水平与对照组比较明显升高,差异有明显统计学意义(P0.001),而盆腔良性疾病组血清HE4水平与对照组比较,差异无统计学意义(P0.05)。在卵巢癌组及盆腔良性疾病组血清CA125水平均升高,与对照组比较,差异有统计学意义(P0.001)。结论结果表明,HE4和CAl25是较理想的卵巢癌肿瘤标志物,其血清水平对卵巢肿瘤的良恶性辅助诊断及鉴别诊断有一定的临床价值,可提高对卵巢癌的诊断能力。     

血清HE4、 CA125及CEA联合检测对卵巢癌诊断价值的评价

目的 评价血清人附睾分泌蛋白4(HE4)、CA125以及CEA联合检测对卵巢癌的诊断价值.方法 收集94例卵巢癌患者,112例卵巢盆腔肿块患者及106例健康对照人群的血清.酶联免疫吸附双抗体夹心法检测HE4的水平,电化学发光法检测CA125和CEA水平.使用二元Logistic回归分析CA125和CEA的联合价值,然后使用受试者工作特征曲线评价其单独和联合诊断的价值.结果 E4、CA125及CEA三项联合评价时,区分健康对照和卵巢癌的灵敏度和特异性分别为93.6％和82.1％,区分良性盆腔肿块和卵巢癌的灵敏度和特异性分别为91.5％和90.2％.与单项指标相比,诊断价值均显著提高,且差异具有统计学意义.结论 HE4、CA125及CEA联合检测可提高卵巢癌的诊断价值,可作为一种潜在的卵巢癌辅助诊断方法.     

Clinical Significance of Detecting Serum HE4, CA125 and CA724 Levels in Diagnoses of Ovarian Malignancies

目的 通过检测卵巢恶性肿瘤患者血清人附睾蛋白4(HE4)、CA125和CA724水平,探讨这三项检测在卵巢恶性肿瘤诊断中的临床价值.方法 采用电化学发光免疫法(ECL)检测CA125和CA724水平,以ELISA法检测HE4水平.测定36例卵巢恶性肿瘤组患者、59例卵巢良性疾病患者及70名健康体检人群血清三项水平并进行比较.结果 卵巢恶性肿瘤组患者血清HE4、CA125和CA724水平显著高于良性疾病组和健康体检组(P＜0.05),其中良性疾病组CA125和CA724水平高于健康体检组(P＜0.05),但是良性疾病组HE4水平与健康体检组比较差异无统计学意义(P＞0.05);卵巢恶性肿瘤组患者血清HE4、CA125、CA724单独检测的灵敏度分别为69.4％、83.3％、58.3％,而这三项联合检测的灵敏度为91.7％,明显高于三项单独检测(P＜0.05).当以卵巢良性疾病组作为参照人群时,HE4、CA125、CA724单独检测的特异性分别为96.6％、62.7％、81.4％,而这三项联合检测的特异性为61.0％.结论 HE4在诊断卵巢恶性肿瘤上具有高度特异性的肿瘤标志物,区分良性或恶性肿瘤时应选择HE4检测;卵巢肿瘤疾病的筛查是可选择HE4、CA125、CA724和三项联合检测以提高诊断的敏感性.     

Level of functioning of siblings and parents of probands of varying degrees of retardation

A further analysis of already published data supports the position that retardates of low ability level less frequently have retarded siblings, retarded parents, and parents low in occupational level than do retardates higher in ability level. The analysis supports the position that there are two types of retarded individuals, persons retarded as a result of gene or chromosomal anomalies, brain injury, etc., who more frequently occur in the lower-level retardate group, and persons whose retardation represents polygenic segregation, who more frequently occur in the higher-level group.     

局部注射辛伐他汀对骨质疏松大鼠股骨髁骨小梁的改建效应

背景：局部注射具有成骨作用的辛伐他汀,可显著增加骨质疏松大鼠股骨颈及股骨髁部的骨密度及力学强度,分析局部注射辛伐他汀对股骨髁骨小梁的影响。 目的：进一步研究骨质疏松大鼠股骨内局部注射辛伐他汀对股骨髁骨小梁的影响。为将辛伐他汀应用于临床骨质疏松局部治疗提供实验基础。 方法：18只雌性SD大鼠双侧卵巢切除后3个月,制备大鼠骨质疏松模型。实验大鼠随机数字表法均分为3组,分别在实验大鼠的右侧股骨髓腔内单次注射辛伐他汀溶液5 mg、10 mg,对照组单纯注射空白载体。分别在注射后1个月处死大鼠并取材。Micro-CT扫描并定量分析骨组织形态变化。 结果与结论：给药后1个月,Micro-CT扫描结果显示,辛伐他汀治疗组的骨微结构参数如骨皮质厚度、骨小梁密度及连接率明显优于对照组。说明疏松骨骼单次注射小剂量辛伐他汀可显著促进股骨髁部骨小梁改建,改善骨骼微结构,可为强化局部、防治骨质疏松骨折的新选择进一步提供实验基础。     

石斛属民族药用植物的分类及生药学研究

中药石斛早在《神农本草经》中就被列为上品,其应用历史悠久,具有养阴生津、补肾益气、润喉护嗓、活血明目、抗癌防老等功效。本文对国内外石斛属民族药用植物的分类及生药学研究进行了综述。     

膝关节骨性关节炎的关节镜治疗体会

目的我科自2001～2005年4月对59例67膝的骨性关节炎（0A），进行关节镜检查及镜下清理术。方法镜检：膝关节骨性关节炎伴不同程度滑膜炎，滑膜皱壁粘连纤维片，关节软骨不同程度破坏。镜下清除增生滑膜，松解粘连，去除剥脱软骨、修复关节面。结果随访2月～4年，平均1.5年，术后综合评估2月～1年，优良率94％；1～2年，优良率79.2％；2年以上优良率54.8％。结论关节镜对膝关节骨性关节炎诊断能提供了比较全面的资料，并对骨性关节炎早期有良好疗效，具有创伤小、恢复快、并发症少和重复治疗等优点。     

Assessment of the efficacy of treatment of hemangiomas

Assessment of the aftereffects of cryoexposure and ultrahigh-frequency cryoexposure on hemangioma tissue of various types, cavernous and squamous, showed a higher cryogenic effect in hemangiomatous tissue preexposed to ultrahigh-frequency waves. A quantitative criterion is proposed for assessing the efficacy of the studied methods of exposure. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 6, pp. 669–672, June, 1995     

Pathways of the realization of immunomodulating effects of neurotransmitters

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 115, N ^o 4, pp. 401–404, April, 1993     

Anatomical study of distribution of valves of the cutaneous veins of adult''s limbs

Since the cutaneous veins of the four limbs have been used as autogenous grafts in the treatment of cardiovascular disorders, knowledge of distribution of the valves is increasingly required depending upon the use. In the gross anatomical study of distribution of valves of the trunci venae, there has been argument about locational relationships between the venous roots and the valves in the vicinity of the roots and the inter-valvular distance. However, there have been only few reports discussing detailed information about valves of the cutaneous veins of the four limbs. The authors observed patterns of distribution of the cutaneous venous valves of the four limbs of cadavers prepared for practice in anatomy. The following parts were excised from each cadaver: the cephalic, basilic, and the great saphenous veins, which originate from the acral venous network and flow into the proximal deep veins, and the venous roots communicating with these veins. An incision was made on each excised vein in the direction of the long axis under observation with a stereoscopic microscope, and the inter-valvular distance and the distance between the valve and the orifice of venous root in the vicinity of the valve were measured. The inter-valvular distance varied with type of the truncus venae, and it varied according to area even in the same truncus venae.     

Characterization of sterols of three digenetic trematodes of buffalo

Sterols of three digenetic trematodes were isolated and characterized by infrared and 1H nuclear magnetic resonance spectroscopies, and gas-liquid chromatography and gas chromatography-mass spectrometry. Sterols identified were cholesterol, cholestanol, 24-methylcholesterol, 24-methylcholestanol, 24-ethyl-22-dehydrocholesterol, 24-ethyl-22-dehydrocholestanol, 24-ethylcholesterol and 24-ethylcholestanol.     

Mechanisms of generation of slow components of bioelectric activity in diagnostics of functional state of the gastrointestinal tract

New electrophysiologic devices were used for the diagnostics of the state of excitable structures in the gastrointestinal tract and correction of their motor functions. Bioelectrical and biomechanical activities form the basis of functioning of internal organs. The mechanisms of generation of slow bioelectrical activity that are important for clinical and physiological studies are described. One of these mechanisms is a capacitance parametric transducer converting the energy of contractions into specific electric signals reflecting muscle functions. Another mechanism results from slow oscillations of resting potentials of interrelated excitable cells in large neuromuscular structures of internal organs. The elaborated procedure is efficient for preventing early postoperative paresis of the gastrointestinal tract. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 128, No. 10, pp. 448–452, October, 1999     

胃小弯侧血管弓的MDCT解剖

目的：研究正常胃小弯侧血管弓及其属支血管的多排螺旋CT表现。方法：回顾性分析51例正常成人的上腹部CT资料,观察胃小弯侧血管弓及其属支血管的显示情况、血管弓形态及其与胃壁的关系。结果：51例均显示胃小弯侧血管弓,血管弓贲门部属支、胃体部属支和幽门部属支分别显示46例、15例和10例;血管弓与胃壁紧邻41例。结论：MDCT检查可良好显示正常胃小弯侧血管弓及其属支血管。