T-lymphocyte subsets in peripheral blood of patients with bone tumors

Peripheral blood T-cell subsets of 54 patients with malignant or benign bone and soft tissue tumors and 20 age-and sex-matched normal controls were studied by using monoclonal antibodies. T-cell subsets were also studied before and after operation in ten patients with osteosarcoma. The number of T cells (OKT 11) and OKT 4 cells significantly decreased in malignant bone and soft tissue tumors. The number of OKT 8 cells increased. Statistically significant decrease in the T4/T8 ratio and the number of macrophage were also found in malignant bone and soft tissue tumors. In giant cell tumor of bone, the number of T cells and OKT 4 cells were also statistically decreased. But, the number of OKT 8 cells and macrophage remained unchanged. The ratio of T 4/T 8 was nearly normal. There was no difference in T cell subsets between benign bone tumors and normal controls. In malignant bone tumors, the number of T cells and T 4/T 8 ratio in some degree may be as a predictor for prognosis.     

The prognostic value of peripheral blood lymphocyte subsets in patients with bladder carcinoma treated using neoadjuvant M-VEC chemotherapy

OBJECTIVES: To assess the prognostic value of peripheral blood lymphocyte subsets in patients with bladder cancer who were treated with neoadjuvant chemotherapy. PATIENTS, SUBJECTS AND METHODS: Thirty patients with a histological diagnosis of invasive bladder transitional cell carcinoma and 30 age-matched controls with no evidence of cancer and immunological disorders were evaluated. Peripheral blood samples were assessed in both groups using monoclonal antibodies. Patients with bladder cancer who achieved complete or partial responses and those who had progression of the disease after systemic chemotherapy with methotrexate, vinblastine, epirubicin and cisplatin were compared according to the pretreatment values of the peripheral blood lymphocyte subsets. RESULTS: There were no significant differences in B lymphocyte levels between the groups. In patients with bladder cancer, the percentages of T lymphocytes (P<0.01), natural killer (NK) cells (P<0.05) and the CD4+/CD8+ ratio (P<0.05) were significantly lower than in the control group. In patients who responded to the chemotherapy regimen, the pretreatment values of T lymphocytes (P<0.001), the CD4+/CD8+ ratio (P<0.01) and NK cell levels (P<0.01) were significantly higher than in the patients who did not. CONCLUSION: In patients with invasive bladder carcinoma, cell-mediated immunity may have a role in the resistance to this malignancy and in these patients the pretreatment levels of T lymphocyte subsets may be an indicator of the potential response to chemotherapy.     

宫颈上皮内瘤变患者淋巴细胞亚群的分析

目的了解宫颈上皮内瘤变(CIN)患者全身细胞免疫功能的变化。方法用特异性荧光标记素,通过流式细胞术对38例CIN患者及20例健康对照组的外周血中T淋巴细胞亚群进行检测。结果随着病变级别加重,CIN患者的CD4+T细胞比例逐渐下降,而CD8+T细胞比例逐渐增加,但差异无统计学意义。与健康对照相比,CIN2患者和CIN3患者的CD4+T细胞/CD8+T细胞的比值下降,分别是(1.46±0.43)、(1.05±0.52)和(0.99±0.58),差异有统计学意义。CD8+T细胞的激活亚群,CD4+T细胞和CD8+T细胞的纯真亚群、记忆亚群在各组之间没有明显的统计学差异。CIN3患者的CD4+T细胞中的激活亚群[(17.8±6.1)%]与健康对照组[(11.5±5.4)%]有显著性差异。结论 CIN患者的全身细胞免疫功能出现异常,为免疫治疗干预癌前病变发展提供了理论基础。     

膀胱肿瘤患者红细胞免疫功能的变化

目的 探讨膀胱肿瘤患者的红细胞免疫功能状态及与Ｔ淋巴细胞亚群改变的关系。方法 测定３４例膀胱移行细胞癌患者的红细胞Ⅰ型补体受体花环率（Ｃ３ｂＲＲ）、免疫复合物花环率（ＩＣＲ）及４项肿瘤红细胞花环率［直向肿瘤红细胞花环试验（ＤＴＥＲ）、促肿瘤红细胞花环试验（ＥＴＥＲ）、协同肿瘤红细胞花环试验（ＡＴＥＲ）及自然肿瘤红细胞花环试验（ＮＴＥＲ）］,同时用流式细胞仪（ＥＴＥＲ）检测Ｔ细胞亚群,与年龄相近的３     

异体手移植术后淋巴细胞亚群的早期监测

目的 探讨手移植患者术后外周血淋巴细胞亚群的变化和意义,以及如何通过CD_3~ 细胞数量的监测指导抗胸腺细胞球蛋白(antithymocyte globulin,ATG)的用量。方法 提取患者的外周血细胞,加入三荧光和双荧光标记的鼠抗人单克隆抗体CD_3/CD_4/CD_8,CD_3/CD(16 56),CD_3/HLA-DR,流式细胞分析仪进行测定。结果 术后一周内CD_3~ ,CD_4~ ,CD_8~ T细胞,活化T细胞[CD_3~ /CD_(16 56,CD_3~ /HLA-DR~ ],静止性T细胞[CD_3~ /CD_(16 56)~-,CD_3~ /HLA-DA~-明显下降,一周后除CD_~ 细胞比术前升高外,均恢复至术前水平;CD_4/CD_8比值持续呈低水平;B细胞(CD_3~-/HLA-DR~ )术后一周内升高,第10天逐渐下降至比术前略高水平;NK细胞(CD_3~-/CD_(16 56)~ )术后1天内显著升高,但第二天直线下降并维持在低水平。结论 两例异体手移植中的ATG剂量(2mg/kg/d)较为适宜,其CD_3~ 细胞数量的监控范围为0.1×10~9～0.4×10~9∧。提示T淋巴细胞亚群的监测有助于判断移植手的存活和诊断排斥反应的发生。免疫抑制剂对移植术后的T淋巴细胞亚群有明显的影响。     

腹膜透析患者腹主动脉钙化与外周血淋巴细胞亚群的相关研究

目的 探讨腹膜透析(peritoneal dialysis,PD)患者腹主动脉钙化(abdominal aortic calcification,AAC)与外周血淋巴细胞亚群之间的关系.方法 收集86例PD患者临床资料,腹部侧位平片判断患者发生AAC的程度,并计算腹主动脉钙化积分(AAC score,AACs),依据患...     

人骨肉瘤外周血T淋巴细胞亚群和自然杀伤细胞免疫变化的研究

目的研究人骨肉瘤患者外周血T淋巴细胞亚群和自然杀伤细胞(NK细胞)的变化。方法采用流式细胞术对42例骨肉瘤、41例骨髓炎、34例健康者的外周血CD4 、CD8 、CD4 、CD4 /CD8 、CD3 、CD16 56均显著性低于骨髓炎患者和健康对照组(P<0.01),而CD8 则升高(P<0.05)。骨髓炎患者外周血CD4 、CD4 /CD8 、CD3 较健康人降低,CD8 则升高,但差异均无统计学意义(P>0.05),而CD16 56与健康对照组相比降低(P<0.05)。结论骨肉瘤患者细胞免疫功能明显降低;骨肉瘤患者的免疫抑制远较骨髓炎的强;骨肉瘤患者NK细胞的降低较骨髓炎患者明显多。流式细胞术可作为检测骨肉瘤患者免疫功能的有效方法。     

恶性肿瘤患者化疗及附加免疫治疗后淋巴细胞亚群的变化

目的：了解恶性肿瘤患者化疗和附加免疫治疗前后淋巴细胞亚群的变化及其临床意义。方法：利用流式细胞仪测定51例化疗前后和26例附加免疫治疗前后的淋巴细胞亚群及其活性。结果：化疗后1周患者CD3^ ,CD4^ 及NK细胞较化疗前明显降低（P＜0．05）,CD8^ ,CD19^ 细胞无明显变化（P＞0．05）,附加免疫治疗组治疗后1周CD8^ 及NK细胞较化疗前明显升高（P＜0．05及P＜0．01）,CD4^ 细胞,CD4^ /CD8^ 比值降低（P＜0．01）。结论：化疗对恶性肿瘤患者细胞免疫功能有明显抑制作用。附加免疫治疗对恶性肿瘤患者细胞免疫功能有明显改善作用。生物反应调节剂能增强化疗患者的细胞免疫功能,恶性肿瘤患者在化疗时应同时使用生物反应调节剂。     

Effect of bariatric surgery on peripheral blood lymphocyte subsets in women

BackgroundThe use of bariatric surgery to treat refractory obesity is increasingly common. The great weight loss that can result from these procedures has been shown to ameliorate certain deleterious effects of obesity. However, the effect of surgery on immune status is unclear. We investigated the relationship between surgical weight loss and peripheral blood lymphocyte percentages in women.MethodsWomen (n = 20, age range 25–59 years, body mass index [BMI] range 36.4–68.2 kg/m²) who had undergone either gastric banding (n = 14) or gastric bypass (n = 6) were enrolled in a prospective study to determine the percentages of their peripheral blood T cells (CD3+, CD4+, and CD8+), CD19+ B cells, and CD3?/CD16+CD56+ natural killer precursor cells before and 85 ± 7 days (3 months) postoperatively using flow cytometry. The data are expressed as the percentage of total lymphocytes ± the standard error of the mean.ResultsA decrease in the BMI at 3 months postoperatively was 12% in the overall study population and 8% and 20% in the banding and bypass groups, respectively. No significant changes were found in the CD4+ or CD8+ T cells (P = .9 and P = .5, respectively), CD19+ B cells (P = .6), or natural killer precursor cells (P = .25) in the overall population or among the patients when stratified by surgical procedure (gastric banding or bypass). The change in CD3+ T cells approached significance (P = .06). A “same direction” (negative) correlation was found between the decrease in BMI and changes in the CD4+ T cell percentages between the pre- and postoperative levels in all the participants, and in the bypass and banding groups separately. However, it only reached statistical significance in the bypass group (r = ?.96, P = .002). When studying the correlation between the decrease in BMI and the changes in CD3+ T cell percentages between the pre- and postoperative levels, a borderline significant negative correlation was found for all participants (r = ?.44, P = .05) and in the bypass group (r = ?.76, P = .08). The rate of change in the CD4+ and CD3+ T cells was greatest among those with the least weight loss and decreased with greater weight loss.ConclusionAn inverse relationship exists between the change in certain T cells (CD4+ and CD3+) and the amount of weight lost after bariatric surgery, mainly gastric bypass surgery. The greater the decrease in BMI, the lower the change in these T cells.     

初治骨关节结核患者外周血T细胞亚群特点及临床意义

目的 探讨初治骨关节结核患者外周血T细胞亚群特点、与临床观测指标的关系及抗结核治疗对患者细胞免疫状况的影响。方法 采用单平台流式细胞术检测骨关节结核患者治疗前和治疗后1-2个月T细胞亚群的绝对数及百分比，并与恶性骨肿瘤患者和健康人比较。结果 初治骨关节结核组CD4及CD8T细胞绝对数较健康对照组低（P〈0．01）。骨关节结核患者在治疗1-2个月后CD4T细胞百分比升高（P〈0．05）。青年组CD8T细胞绝对数和百分比显著高于中年组和老年组（P〈0．05，P〈0．01），CD4／CD8显著低于中年组及老年组（P〈0.01）。T细胞亚群各参数与PPD反应直径、红细胞沉降率和C反应蛋白之间无显著相关性（P〉0．05）。脊柱结核合并脓肿组CD4／CD8较低，CD8T细胞百分比较高（P〈0．01）。结论 初治骨关节结核患者存在细胞免疫缺陷，以中老年组更明显。脊柱结核是否形成脓肿与CD8T细胞有关。初治骨关节结核患者经1～2个月化疗后，细胞免疫状况改善，是病灶清除术的有利时机．     

Correlation between radiculalgia and counts of T lymphocyte subsets in the peripheral blood of patients with lumbar disc herniation

Objective: To determine the correlation between the degree of radiculalgia and counts of T lymphocyte subsets in the peripheral blood of patients with lumbar disc herniation. Methods: Forty‐nine patients with lumbar disc herniation (group A) were divided into three subgroups according to Visual Analogue Scale (VAS) pain scores (group A₁: n= 12, VAS 0–4.0; A₂: n= 24, VAS 4.1–7.0; A₃: n= 13, VAS 7.1–10.0. Twenty health blood donors who volunteered to be involved in the study comprised the control group (group B). Peripheral blood counts of various T lymphocyte subsets were measured in each group. Results: (i) The counts of CD4+ T and CD4+/CD8+ lymphocytes were higher in group A than in group B, and the difference between the two groups was statistically significant (P < 0.05). There were also statistically significant differences between group A and group B in the counts of CD3+ and CD8+ T lymphocytes (P < 0.05); (ii) There was no correlation between the VAS scores and the counts of CD3+ T lymphocytes (r= 0.194, P > 0.05). A strong significant correlation was observed between the VAS scores and counts of CD4+ T lymphocytes (r= 0.542, P < 0.05), CD4+/CD8+ (r= 0.468, P < 0.05), which increased with increasing VAS scores in the three subgroups of group A (P < 0.05). However there was a significant negative linear correlation between CD8+ T lymphocyte counts and pain scores (r=?0.462, P < 0.05). Conclusion: Our results suggest that changes in T lymphocyte subsets in peripheral blood take place after prolapse of lumbar intervertebral discs. The current results may provide support for involvement of immunologic mechanisms in low back pain secondary to herniation of the lumbar disc. T lymphocytes may play an important role in the development of symptoms in patients with lumbar intervertebral disc herniation.     

七氟醚和丙泊酚对HIV感染患者外周静脉血T淋巴细胞亚群的影响

目的探讨七氟醚和丙泊酚全身麻醉对HIV感染患者外周静脉血T淋巴细胞亚群的影响。方法选择全麻下接受妇科及结肠肿瘤手术的HIV感染患者28例,男16例,女12例,年龄28～65岁,体重50～75 kg,ASAⅠ—Ⅲ级。随机分为两组:七氟醚组(S组)和丙泊酚组(P组),每组14例。S组术中持续吸入2%～4%七氟醚,P组持续静脉注射丙泊酚3～6 mg·kg~(-1)·h~(-1),两组均泵注瑞芬太尼0.4～0.6μg·kg~(-1)·min~(-1),调节麻醉深度,维持术中BIS值在40～60。分别于麻醉前30 min(T_0)、气管插管时(T_1)、切皮时(T_2)、手术结束时(T_3)及拔管时(T_4)记录患者生命体征和BIS值以及手术时间和术中出入量。分别于T_0、T_3、术后24 h(T_5)及术后3 d(T_6)抽取患者外周静脉血,检测T淋巴细胞亚群(CD3~+,CD4~+,CD8~+)的含量,计算CD4~+/CD8~+比值,并检测T_0、T_5和T_6时HIV病毒载量。结果与T_0时比较,T_3时两组外周静脉血CD3~+、CD4~+含量和CD4~+/CD8~+比值明显降低,CD8~+含量明显增加(P0.05)。与P组比较,T_3、T_5时S组CD3~+、CD4~+含量和CD4~+/CD8~+比值明显增高,CD8~+含量明显降低(P0.05)。两组HIV病毒载量差异无统计学意义。结论相同麻醉深度下,与丙泊酚比较,七氟醚全身麻醉对HIV感染患者T淋巴细胞亚群抑制较轻且恢复较快。     

脾切除对乙型肝炎肝硬化患者外周血T细胞及其活化抗原HLA-DR的影响

目的：检测乙型肝炎肝硬化脾切除患者外周血T细胞活化抗原HLA-DR的表达水平,探讨其临床意义。方法：以28例乙型肝炎肝硬化脾切除患者为实验组（肝硬化脾切除组）,同时以38例乙型肝炎肝硬化非脾切除患者和22例健康人为对照组（肝硬化非脾切除组、健康组）。采用流式细胞技术检测外周血T淋巴细胞亚群及HLA-DR抗原的表达。结果：肝硬化脾切除组患者外周血CD3＋、CD4＋和CD8＋百分含量分别为（41.59±6.79）%,（22.64±6.87）%和（19.16±7.28）%,显著低于健康组和肝硬化非脾切除组。肝硬化脾切除组患者CD4＋/CD8＋的比值显著高于健康组;HLA-DR在CD3＋、CD4＋和CD8＋上的表达水平分别为（25.42±5.51）%,（22.36±4.90）%和（28.53±8.35）%,与健康组相比差异无统计学意义,但在CD3＋和CD8＋上的表达水平显著低于肝硬化非脾切除组。结论：肝硬化脾切除患者存在细胞免疫功能缺陷,表现为T细胞数量的减少和活化功能的下降。     

膀胱肿瘤患者的凝血象研究

为探讨凝血象在膀胱肿瘤患者中的改变，分别采用全自动血细胞分析法，血凝法和定量酶联免疫吸附法对膀胱肿瘤组凝血象指标，包括血小板（ＰＬＴ），血小板平均体积（ＭＰＶ），血小板比积（ＰＣＴ）和血小板平均宽度（ＰＤＷ）以及凝血酶原时间（ＰＴ），部分凝血活酶时间（ＡＰＴＴ），纤维蛋白原（Ｆｂｇ）和Ｄ－二聚体予以测定，并与非肿瘤组进行比较，结果膀胱肿瘤线ＰＬＴ较非肿瘤组明显增高（Ｐ〈０．０１），ＭＰＶ降低（Ｐ〉     

Effect of lentinan on lymphocyte subsets of peripheral blood,lymph nodes,and tumor tissues in patients with gastric cancer

The effect of lentinan on lymphocyte subsets of peripheral blood, lymph nodes, and tumor tissues in gastric cancer patients was investigated. A 2-mg dose of lentinan was administered to 12 patients intravenously twice, the first at 3–9 days before and the second the day before surgery. The results were then compared with a control group without lentinan administration comprising 12 patients. Regarding peripheral blood and lymph nodes without metastasis, lymphocyte subsets defined with anti-CD3, anti-CD4, anti-CD8, anti-Leu7, and anti-Leu11 were analyzed by flowcytometry. As for tumor tissues, lymphocyte subsets defined with anti-CD3, anti-CD4, anti-CD8, anti-Leu11, and anti-M3 were analyzed after immunohistochemical staining. There were no significant changes in the lymphocyte subsets of peripheral blood between the two groups. In the lymph nodes, the CD4 cell ratios increased; otherwise in regard to tumor-infiltrating lymphocytes (TILs), the number of CD4, Leu11 and LeuM3 cells showed a prominent increase. Therefore, lentinan was observed to produce different effects in accordance with the subjective organs.     

泌尿系肿瘤患者T淋巴细胞核仁组织区嗜银蛋白应用价值的研究

目的探讨外周血T淋巴细胞核中的rDNA转录活性检测在泌尿系肿瘤患者中的应用价值。方法肾癌患者21例,TNM分期:T1N0M06例,T2N0M09例,T3N0M04例T4N2M12例;膀胱癌患者42例,TaN0M014例,T1N0M011例,T1N0M11例,T2N0M08例,T3N0M04例,T4N0M04例;常规体检正常者28例为对照组;血液透析治疗尿毒症患者23例作为阳性对照组。取外周血检测T淋巴细胞核仁银染面积与核面积的比值(AgNORs),比较组间差异和影响因素。结果泌尿系肿瘤和尿毒症患者外周血T淋巴细胞AgNORs含量显著低于正常对照组(8.55±1.11)%,P<0.01;肾癌患者T淋巴细胞AgNORs含量为(5.44±0.68)%,膀胱癌患者(5.42±0.68)%,显著低于尿毒症患者(6.29±0.97)%,P<0.01,且70%患者为低活性表达(<6.00%);术后2周内无显著性改变。外周血T淋巴细胞AgNORs含量与肿瘤分期分级、肾癌肿瘤体积、膀胱肿瘤细胞浸润能力及肿瘤复发次数等无显著相关。结论外周血T淋巴细胞AgNORs值可用于泌尿系肿瘤的免疫监测,有可能作为泌尿系肿瘤的初筛检查。     

Prognostic value of peripheral blood T lymphocyte subsets in clear cell renal cell carcinoma

BackgroundTo date, few studies have evaluated the role of peripheral blood T lymphocyte subsets in patients with clear cell renal cell carcinoma (ccRCC). Here we measured the levels of peripheral blood T lymphocyte subsets and evaluated its prognostic value in ccRCC.MethodsData from 122 patients with RCC from January 2018 to January 2020 were collected. Preoperative peripheral blood T lymphocyte subsets and medical records were analyzed. Kaplan-Meier cures and log rank test were used for analyzing overall survival (OS). Univariate and multivariate survival analyses were underwent by performing the Cox proportional hazards models. Correlations were tested by Pearson’s correlation analysis.ResultsOf 122 patients, a total of 80 ccRCC patients was enrolled. Patients with low CD3⁺ T cells and low CD4⁺/CD8⁺ ratio displayed a worse OS than patients with high CD3⁺ T cells and high CD4⁺/CD8⁺ ratio (P=0.029 and 0.002, respectively). Multivariate analyses showed CD3⁺ T cells and CD4⁺/CD8⁺ ratio were independent predictive factors for the OS (HR: 0.295, 95% CI, 0.091–0.956; P=0.042 and HR: 0.244, 95% CI, 0.065–0.920; P=0.037, respectively). Moreover, NLR negatively correlated with both levels of CD3⁺ T cells and CD4⁺/CD8⁺ ratio (P<0.001, r=−0.398 and P=0.012, r=−0.280, respectively).ConclusionsThe findings of our study suggest that preoperative CD3⁺ T cells and CD4⁺/CD8⁺ ratio in peripheral blood are independent predictors for patients with ccRCC.