大鼠马桑内酯全身型点燃效应癫痫模型

作者用亚抽搐量的马桑内酯作反复肌肉注射,造成了大鼠点燃效应癫痫模型。这是一种较理想的慢性实验性癫痫模型。     

马桑内酯致大鼠点燃效应癫痫间期蓝斑去甲肾上腺素荧光...

Fourteen male Wistar rats were divided into two groups. The experimental rats were injected with subconvulsive dosage of coriaria lactone (1 mg/kg) intramuscularly per 3.5 days. The controls were injected with normal saline. After 26 injections, the loci coeruleus of kindling rats were studied with the noradrenaline (NA) fluorescence histochemical technique at the time between seizures. The NA fluorescence could be clearly visualized under fluorescent microscope. The intensity of fluorescence was reflected by autoexposure-meter of the fluorescent microscope. The brighter the fluorescence, the shorter the autoexposure time. The intensity of NA fluorescence in the locus coeruleus of experimental animals was weaker than that of the controls. Since NA plays an inhibitory role in cerebral cortex, the decrease of NA, either induced by repeated injections of coriaria lactone or due to the time of sample taken after seizure, needs further study.     

大鼠马桑内酯急性局灶型癫痫大脑运动皮质的形态计量研究

用微量马桑内酯注入Ｗｉｓｔａｒ大鼠左侧前肢运动皮质，造成急性局灶型癫痫。用光镜、电镜和体视学方法研究其运动皮质第Ｖ层结构的改变。结果显示：癫痫大鼠运动皮质灶区、灶旁区的神经细胞数和胶质细胞数均分别比对照大鼠灶区和灶旁区显著减少；灶区神经毡中突触性终末数，显著减少；突触性终末的面积分数明显减少，而树突的面积分数无变化；神经胶质突起的面积分数增加。     

SC1001钠盐对马桑内酯所致家兔海马点燃性癫痫发作的影响

Healthy rabbits weighing 1.7-2.4 kg were used. According to Sawyer's atlas the stainless steel cannula and recording electrode were implanted into the left hippocampus of each animal. The kindling experiments were started one week after the surgical operation. For kindling, all animals received injection of 2 microliters of diluted solution containing 0.55 or 0.32 microgram of coriaria lactone (CL) every second day, through the single stainless steel cannula previously implanted in the hippocampus. Hippocampal EEG of all the animals was recorded by telemetric method and the behavior of them was observed at the same time. Eighteen fully kindled rabbits were separated into three groups. The SC1001 Na group of six rabbits was treated with SC1001 Na (100 mg/kg, i.m.), the PB Na group (six rabbits) received phenobarbital Na (30 mg/kg, i.m.), and the control group of six was treated with placebo (normal saline, i.m., in the same volume as that of the SC1001 Na injection); injections were administered once a day for two courses of treatment (one course of treatment was 1 week). The results of the experiment indicated that SC1001 Na (100 mg/kg) had certain effects on hippocampal kindled seizures induced by CL, but its curative effects were not more notable than those of PB Na (30 mg/kg).     

马桑内酯致大鼠点燃效应癫痫间期蓝斑去甲肾上腺素荧光组织化学的研究

用去甲肾上腺素(NA)荧光组化法研究马桑内酯致大鼠点燃效应癫痫发作间期的蓝斑,荧光强度通过显微摄影的自动曝光系统进行相对定量。结果显示:点燃效应癫痫发作间期蓝斑NA比对照组减少,作者认为NA的抑制作用减弱是导致癫痫的原因之一。     

马桑内酯癫痫大鼠海马的形态计量研究

用１０只健康成年ＳＤ大鼠，５只实验鼠在左侧大脑皮质前肢运动区注射ＣＬ３．８μｌ诱发癫痫；５只对照鼠注射等量生理盐水、无癫痫发作。在癫痫鼠发作６０分钟后与对照鼠同时取脑，比较海马的结构。发现癫痫鼠海马ＣＡ１区锥体细胞粗面内质网、溶酶体和线粒体的面积分数显著增加，表明癫痫发作６０分钟，海马ＣＡ１区锥体细胞已发生了某些超微结构改变，这为进一步研究癫痫脑神经元损伤的可复性打下了基础。     

SC1001钠盐对马桑内酯所致家兔海马点燃性癫痫发作的影响

作者用点燃效应模型对SC1001钠盐的抗痫作用进行了观察。实验结果表明,此药对马桑内酯所致家兔海马点燃性发作有一定的对抗作用,但肌肉注射SC1001钠盐100mg/kg的疗效不如肌肉注射苯巴比妥钠30mg/kg显著。     

大鼠马桑内酯体运动局部癫痫持续状态模型

作者用含马桑内酯0.16mg的明胶淀粉微粒包埋于大鼠大脑皮质运动区,造成了体运动局部癫痫持续状态模型。为研究马桑内酯致癫痫的机理提供了一个维持时间长的模型。     

马桑内酯癫痫大鼠海马的形态计量研究

用１０只健康成年ＳＤ大鼠，５只实验鼠在左侧大脑皮质前肢运动区注射ＣＬ３．８μｌ诱发癫痫；５只对照鼠注射等量生理盐水，无癫痫发作。在癫痫鼠发作６０分钟后与对照鼠同时取脑，比较海马的结构。发现癫痫鼠海马ＣＡ１区锥体细胞粗面内质网、溶酶体和线粒体的面积分数显著增加，表明癫痫发作６０分钟，海马ＣＡ１区锥体细胞已发生了某些超微结构改变，这为进一步研究癫痫脑神经元损伤的可复性打下了基础。     

马桑内酯局部癫痫持续状态大鼠大脑皮质的形态计量研究

用马桑内酯明胶淀粉微粒包埋于１０只大鼠左侧大脑半球的前肢运动区，诱发局部癫痫持续状态。发作高峰（６小时）时取材，用光镜、电镜和体现学方法研究大脑皮质运动区第Ⅴ层的形态、结构、细胞数量及神经毡成分的变化。与对照鼠比较，癫痫鼠大脑皮质第Ⅴ层神经元的数目减少，以灶区下降最多，灶旁区次之；胶质细胞数也明显减少，各区间下降程度相近。癫痫鼠皮质神经毡的突触性终末数及其面积分数明显下降，以灶区为甚，灶旁区次之；而胶质突起的面积分数却明显增加，以灶区为甚，镜灶区次之。     

马桑内酯致小鼠癫痫脑内环磷酸腺苷的改变

作者给小鼠肌肉注射马桑内酯2.5mg/kg,引起重型癫痫发作,测定小鼠皮层和海马的环磷酸腺苷(cAMP)水平。结果表明:在癫痫发作之前和轻型发作时,小鼠皮层和海马的cAMP含量无明显改变;重型发作和发作结束后,小鼠皮层和海马的cAMP水平显著增加。此结果提示癫痫发作时脑内cAMP水平增加,很可能是发作的结果而不是引起发作的原因。     

钙通道阻滞剂维拉帕米对马桑内酯所致家兔海马点燃性癫痫发作的影响

作者用马桑内酯所致家兔海马点燃模型,观察了钙通道阻滞剂维拉帕米对痫性活动的影响。实验结果表明,海马内直接注入维拉帕米对家兔点燃性癫痫发作有对抗作用,但肌肉注射效果差,这可能与该药不易通过血脑屏障有关。     

SC1001钠盐对马桑内酯点燃家兔脑神经细胞超微结构的观察

本文报道用马桑内酯阈下剂量反复注入家兔海马造成点燃效应,再用SC1001钠盐治疗,观察其海马及大脑皮层神经细胞超微结构的变化。结果表明:SC1001钠盐能使马桑内酯点燃家兔已出现的神经损害有一定的恢复。     

左乙拉西坦对马桑内酯致痫海马神经细胞钠电流的影响

目的 探讨左乙拉西坦 (Levetiracetam, LEV) 对马桑内酯(coriaria lactone, CL)致痫SD大鼠海马神经元钠电流增加的影响.方法 利用膜片钳全细胞模式, 记录急性分离后CL致痫的大鼠海马神经元钠电流,并给予LEV进行干预,分为对照组、LEV 150 μmol/L组、LEV 300 μmol/L组和未处理组.另用LEV 300 μmol/L预处理40 min后再给予CL致痫,观察钠电流的变化情况.结果 CL致痫后钠电流增加,加入LEV 150 μmol/L后最大电流峰值增加了36.92%±2.84%(P＜0.05),与未处理组比较差异无统计学意义(P＞0.05).LEV 300 μmol/L组钠电流增加了16.58%± 1.56%(P＞0.05);但与对照组相比,差异无统计学意义(P＞0.05).经LEV预处理后,CL仍使钠电流增加了32.86%±6.73%(P＜0.05),与未处理组比较差异无统计学意义(P＞0.05).结论 LEV未能抑制CL致痫海马细胞钠电流的增加,其抗癫痫作用可能不是通过抑制钠电流而产生的.     

马桑内酯对大鼠海马神经元钠电流的影响

目的 研究马桑内酯(coriaria lactone,CL)对大鼠海马神经元钠离子通道电流的影响,探讨该影响在CL致痫中的意义.方法 利用全细胞膜片钳技术,在急性分离的大鼠海马神经元上记录钠电流,观察CL对电流幅度的影响.结果 经0.1 mg/mL、0.2 mg/mL的CL作用后,海马神经元钠电流有不同程度的增加[CL 0.1 mg/mL组的最大峰值电流密度为(-90.11±14.02) pA/pF,增幅为17.32%±8.52%;CL 0.2 mg/mL组的最大峰值电流密度为(-111.52±6.65) pA/pF,增幅为37.98%±4.91%];经与对照组相比,CL 0.2 mg/mL组(P＜0.05)的变化较CL 0.1 mg/mL组(P＞0.05)明显.结论 CL使海马神经元电压依赖性钠电流的幅度增大,从而改变细胞的兴奋性,引发异常放电.