Comparison of R128Q mutations in human, ovine, and chicken leptins

Human leptin and its R128Q mutant, as well as the R128Q mutants of ovine and chicken leptins, were prepared, expressed in Escherichia coli, refolded, and purified to homogeneity yielding electrophoretically pure, over 95% monomeric protein. R128Q mutations did not change the binding properties to BAF/3 cells stably transfected with the long form of human leptin receptor compared, respectively, to non-mutated human, ovine, and chicken leptins. In contrast, the biological activity tested in a proliferation assay in the same cells was drastically changed. Human leptin R128Q lost its activity and even became a weak antagonist, whereas the activities of ovine and chicken leptins were reduced 25- and 80-fold. If dimerization models were applicable leptin receptor activation, the present results would suggest that site 2 of the hormone was impaired. Two models, the human growth hormone:human growth hormone receptor (hGH:hGHR) (1:2) and the granulocyte-colony stimulating factor:granulocyte-colony stimulating factor receptor (GCSF:GCSFR) (2:2) complexes, were used for modeling. Superimposing the leptin structure on the hGH and GCSF models in the complex structures did not indicate any role for R128 in receptor binding. This made it impossible to correlate the results shown in the present work with the currently available models. Therefore, leptin may bind its receptors in a manner different than those proposed until now.     

Surgery for incomplete atrioventricular canal. Mid-term follow-up apropos of 128 patients

Among 128 consecutive patients operated upon between 1972 and 1984 at the Marie-Lannelongue Surgical Centre for incomplete persistent atrioventricular canal, 102 could be followed up for periods ranging from 18 months to 11 years. Of the 23 patients lost sight of, 19 were foreigners. One patient died during the first postoperative month and two died during the following five months; there was no death thereafter. One patient developed complete atrioventricular block immediately after surgery. At the end of the follow-up period, three patients had been reoperated upon for disinsertion of the patch on the ostium primum associated in one case with massive mitral regurgitation; two complete atrioventricular blocks requiring permanent pacing had occurred some time after surgery; five patients had presented with late atrial dysrhythmia, and only six patients had signs of heart failure primarily due to residual mitral regurgitation. At radiography, the cardiothoracic ratio was reduced in 74 p. 100 of the cases from 0.60 +/- 0.08 to 0.53 +/- 0.05 (p less than 0.001), and the earlier the operation had been performed, the greater the reduction in cardiothoracic ratio (p less than 0.05). A mitral regurgitation murmur was observed in 72 p. 100 of patients immediately after surgery and in 82 p. 100 of patients at the end of the follow-up period. The murmur was usually weak (79 p. 100) with a tendency to remain stable (44 p. 100) or to increase in intensity (43 p. 100, but reoperation was necessary in only one case.(ABSTRACT TRUNCATED AT 250 WORDS)     

潘南金辅助治疗慢性重型肝炎 128例效果分析

目的总结潘南金在慢性重型肝炎病人治疗中的作用。方法将2000年1月至2002年9月诊断为慢性重型肝炎的患者188例随机分为治疗组与对照组，治疗组用综合治疗加潘南金30ml 10％葡萄糖液250ml静脉点滴，每天1次，连用30d，对照组仅用综合治疗不用潘南金，分析比较两组病人临床疗效。结果潘南金治疗组病人有效率82．03％(显效56．25％ 有效25．78％)，对照组有效率为68．33％(显效30．00％ 有效38．33％)，电解质紊乱发生率治疗组8．59％，对照组38．33％，并发症发生率治疗组23．44％，对照组41．67％，肝性脑病发生率治疗组10．94％，对照组30．00％，潘南金治疗组均优于对照组。结论潘南金在辅助治疗慢性重型肝炎病人中可以提高临床治疗效果。     

Disulfiram for the treatment of alcoholism. An evaluation in 128 men.

One hundred twenty-eight alcoholic men were assigned randomly to receive either a regular dose of disulfiram (250 mg), a pharmacologically inactive dose (1 mg), or no disulfiram. There were no statistically significant differences among the three treatment groups in total abstinence, percentage of drinking days, days worked, family stability (living with same relative), or percent of scheduled appointments kept. However, 21% of those who received the regular dose of disulfiram and 25% who received the pharmacologically inactive dose remained abstinent, whereas only 12% of those who received no disulfiram did so. These results indicate that disulfiram may be of limited value in the treatment of alcoholism, fear of the disulfiram-ethanol reaction is important in preventing drinking, and patients willing to take disulfiram are more likely to be abstinent if given the drug. We also found that complete abstinence correlated significantly with compliance and obtaining employment.     

Hodgkin disease: An analysis of 128 cases

An analysis of 128 cases of Hodgkin disease seen at the Department of Pathology, All India Institute of Medical Sciences, New Delhi, during the period 1968–1974 is presented with reference to histopathological and clinical findings. A male preponderance is noted in all age groups, and this is most noticeable in children under 15 years of age. There is a predominance of cases with the histologic subtypes associated with a poor prognosis (mixed cellularity and lymphocyte depleted). Only nine cases of nodular sclerosis type were seen.     

Epitopic structure of Tn glycophorin A for an anti-Tn antibody (MLS 128).

Glycophorin A was digested with glycoprotease (Pasteurella haemolytica) and the digest was fractionated by a combination of high-pressure column chromatographies to produce the glycopeptides GPA-1 to GPA-6. Sequence analysis of the glycopeptides revealed that two serine residues (Ser-14 and Ser-15) are not glycosylated, Thr-17 and Ser-19 being glycosylated instead, in disagreement with the accepted structure. The glycopeptides thus obtained were treated with sialidase and beta-galactosidase. The Tn antigenicity, as assayed by the binding to a monoclonal anti-Tn antibody (MLS 128), was found exclusively in the glycopeptides including three (cluster I) or four (cluster II) consecutive residues of GalNAc-Ser/Thr, whereas the glycopeptide (GPA-2) containing two nonconsecutive GalNAc-Ser/Thr residues had practically no Tn antigenicity. The immunoreactivities of GPA-1 and GPA-3, containing both clusters I and II, and GPA-4, containing cluster II, were 63% (calcd. 67%), 81% (calcd. 86%), and 50% (calcd. 50%), respectively, of the immunoreactivity of GPA-5 or GPA-6, containing cluster I (the average being taken as the basis), based on the reactivity per GalNAc residue. These results indicate that clusters I and II react with the antibody to the same extent. The structure consisting of three consecutive glycosylated Ser/Thr residues may be essential for Tn antigenicity in the light of previous results for ovine submaxillary mucin.     

鼻内镜下手术治疗慢性鼻窦炎鼻息肉128例

目的 探讨鼻内镜下慢性鼻窦炎、鼻息肉的手术疗效.方法 对实施内窥镜鼻窦手术并完成术后随防6个月以上的128例患者进行病历资料分析.结果 治愈率(症状完全消失) 79.7%,好转率15.6%,无效率4.7%,总有效率95.3%.结论 鼻内镜手术治疗慢性鼻窦炎、鼻息肉有较好的效果.做中鼻甲和鼻中隔偏曲的处理和术前病情评估及加强术后的综合治疗是提高治愈率的关键.     

静滴地尔硫治疗冠脉重建术围手术期心绞痛128例疗效分析

目的观察静滴地尔硫     

Hybrid seton for the treatment of high anal fistulas: results of 128 consecutive patients

Background

The aim of this study was to evaluate our experience in managing high anal fistulas with a simple modification of the cutting seton.

Methods

We performed a retrospective review of standardized patient charts and of prospectively collected scores and questionnaires. Surgical outcomes of 128 consecutive, well-documented patients with high anal fistulas, including anterior transsphincteric fistulas in females, treated using a hybrid seton, were analyzed.

Results

No significant complications occurred. The mean postoperative pain scores on a visual analog scale were 3.23 and 0.61, on days 1 and 7, respectively. Complete healing was achieved in 67 cases (52.3 %) at 1 month and in all cases (100 %) at 3 months. Recurrent fistula was noted in 2 patients (1.5 %) at 6 and 12 months. The mean postoperative incontinence scores at 3 and 12 months did not differ significantly from the preoperative score (p = 0.061, Wilcoxon’s test). The depression, life style, and embarrassment item scores of the fecal incontinence quality of life index improved significantly after surgical treatment.

Conclusions

The results of this series suggest that the hybrid seton might be a valid alternative for the treatment of high anal fistulas, eliminating the need for postoperative adjustments. The slow and stable cutting of the sphincter seems to have a positive effect on the maintenance of continence. The successful outcome is associated with significant improvement in quality of life.     

静滴地尔硫(艹卓)治疗冠脉重建术围手术期心绞痛128例疗效分析

目的　观察静滴地尔硫 艹卓 对冠脉血管重建术围术期心绞痛的临床有效性和安全性。方法　对 12 8例行冠状动脉血管重建术围术期顽固性心绞痛患者给予静滴地尔硫 艹卓 治疗。通过观察心绞痛、血压和心率变化及2周内心脏急性事件来判定疗效。结果　 (1)心绞痛发作情况 :91例在用药期间未再有心绞痛发作 ,37例心绞痛程度明显减轻。 (2 )血压、心率 :与用药前相比 ,用药 30min内均明显下降 ,1h、2h、6h、12h时间段均分别较其前的时间点下降 ,P <0 0 5 ,但用药后 2 4h、4 8h与用药后 12h相比无显著下降 (P >0 0 5 )。 (3)病人转归 :12 8例中 85例接受了介入治疗 ,手术全部成功 ,术后 1～ 3周内出院 ;4 3例接受CABG ,4 1例手术成功出院 ,2例CABG术后 72h死于室颤及泵衰竭。 (4 )所有患者用药期间未见到药物相关的副作用。结论　对于其它内科药物治疗无效的冠脉重建术围手术期的顽固性心绞痛 ,联合应用静脉滴注地尔硫 艹卓 可以取得较为良好的、安全的效果 ,临床上具有实用性 ,值得推广。     

E-selectin S128R polymorphism leads to severe asthma

The E-selectin mediates the interaction of activated endothelial cells with leukocytes and plays a fundamental role in the pathogenesis of asthma. It has been suggested that an S/R (Serine128Arginine) polymorphism of E-selectin alters ligand binding function. Our purpose in this study was to determine whether this Serine128Arginine polymorphism influences the risk of asthma and also to analyze the possible correlation of disease severity in Iranian patients with polymorphism of E-selection. We studied human E-selectin gene polymorphism in 172 asthmatic patients and 173 healthy volunteers by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). To determine the severity of the asthma's situation, a questionnaire was prepared requesting the following information: age, sex, clinical signs and symptoms and past medical history. After the participants filled in the questionnaire, all active or ex-smoker patients were excluded. A trained observer assessed airway reversibility, peak flowmetry and spirometry in asthmatic patients. We found increased serum levels of soluble E-selectin (sE-selectin) in asthmatic patients compared with healthy subjects (P<0. 0001). Frequencies of the SS, SR, and RR genotypes were found as 66.3%, 31.4%, and 2.3% in the patients and 91.9%, 8.1%, and 0.0% in control subjects, respectively. The 128Arg allele was more prevalent in patients than controls (OR 5.78; 95% CI, 3.07-10.86, P<0.0001). However, in this study the polymorphism was not associated with circulating sE-selectin levels. We found a direct correlation between the level of sE-selectin and the severity of asthma (P=0.001). On the other hand, there was a close relation between 128Arginine carriage and disease severity (P<0.0001). These results suggest that the Ser128Arg polymorphism of the E-selectin gene is a genetic factor that may be associated with the severity of asthma.     

血浆E-选择素及基因S128R多态性与山西汉族脑梗死患者的关系

目的检测脑梗死患者血浆E-选择素水平及基因S128R多态性,探讨其与脑梗死的关系。方法脑梗死组73例,对照组85例,用PCR-RFLP法对DNA多态性进行分析,ELISA法检测血浆E-选择素水平。结果发现3个基因型(SS、SR、RR),其基因型频率和等位基因频率在脑梗死组和对照组比较,差异均有统计学意义(P0.05),相对风险度分析,R等位基因携带者患脑梗死的风险性是S等位基因的4.958倍;脑梗死患者血浆E-选择素水平高于对照组(t=13.139,P0.05),且脑梗死合并高血压、高脂血症患者的血浆E-选择素浓度高于无并发症组(P0.05)。结论E-选择素基因S128R多态性与山西汉族人群脑梗死的发病相关,R等位基因可能是山西汉族人群脑梗死发生的危险因素之一;脑梗死患者血浆E-选择素水平明显升高,且浓度与有无合并高血压和高脂血症密切相关。     

小儿川崎病128例心电图分析

目的 探讨川崎病患儿的心电图改变及临床意义.方法 收集本院128例川崎病患儿进行常规12导心电图检查.结果 128例中,正常心电图42例(32.8％),异常心电图86例(67.2％).主要为窦性心律失常,其次为各类传导阻滞及不同程度ST-T改变.结论 128例中绝大部分患儿心电图有异常改变.提示明确记录到心电图改变对小...