平萎口服液与辨证分型治疗胃粘膜癌前病变的临床对…

本研究应用自制中药平萎１号、秤萎２号口服液治疗６０例胃膜癌前病变（治疗组），并以传统医辩证分型治疗３０例为对照组，结果表明，临床疗效治疗组分别为９３．３％和９６．７％，对照组为９０％，两者对比：Ｐ〈０．０５，Ｐ〈０．９。胃镜复查病理不典型增生有效效率组分别为８０％和８６．７％。对照组为６３．３％，两者相比有显著性差异Ｐ〈０．０５，Ｐ〈０．０１，表明三组中以中医益气养阴药与活血祛瘀抗癌药同时治疗胃粘     

维甲酸对大鼠实验性腺胃粘膜癌前病变的逆转治疗作用

本文旨在观察维甲酸对大鼠胃粘膜癌前病变的作用，结果示，维甲酸治疗组大鼠腺胃粘膜上皮化生、中重度异型增生和胃癌的发生率（７２．０％、２４．０％、０％）均显著地低于未加治疗的对照组（１００．０％、５２．０的、１６．０％）（Ｐ＜０．０１、Ｐ＜０．０５）；治疗组大鼠幽门腺和胃底腺平均肠化腺体数（１３０．２±９３．９、５１．５±３９．１）及硫酸粘液阳性肠化发生率（３６．０％）亦显著地低于未加治疗的对照组（４４２．４±２３０．０、２４７．４±１１２．０，８４．０％）（Ｐ＜０．０１），本实验中未观察到维甲酸明显的毒副作用，说明维甲酸对大鼠实验性腺胃粘膜癌前病变有良好的治疗作用；这为探讨人胃癌前病变的治疗提供了新途径。     

萎平舒胶囊治疗慢性萎缩性胃炎实验研究

目的 :探讨萎平舒胶囊治疗慢性萎缩性胃炎 (CAG)的作用机制。方法 :采用综合法制备大鼠 CAG模型 ,设正常对照组、模型组、三九胃泰阳性对照组以及萎平舒胶囊治疗组 ,观察大鼠体重、胃液中游离酸和胃蛋白酶活性以及胃粘膜病理组织学改变。结果 :萎平舒治疗 6 0 d,与模型组比较 ,大鼠的体重差异无显著改变 ;胃液游离酸显著升高 (P <0 .0 5 ) ,胃蛋白酶活性升高 ,但无统计学差异 ;能明显改善胃粘膜上皮细胞形态异常及腺体萎缩 ,使血管扩张完全消除 ,胃粘膜炎细胞浸润显著减少 ,变薄的胃粘膜显著增厚趋向正常。结论 :萎平舒胶囊对 CAG具有较好的治疗作用     

胃粘膜癌前病变中医药治疗的临床研究

慢性萎缩性胃炎伴癌前病变44例,采用辩证论治加化瘀、抗癌法,中药胃康胶囊法,结合法等3种治疗方法。经胃镜复查31例。结果表明,总有效率83.9％,消失率61.3％;对中度异型增生的消失率为80.0％。癌前病变的中医证型,以脾胃虚寒型、脾胃气滞型和脾胃湿热型的疗效较明显。初步证明了用中医药阻断癌变的可能性。     

健胃灵口服液防治胃癌癌前病变的实验研究

采用Ｎ－甲基－Ｎ‘０硝基－Ｎ亚硝基胍（ＭＮＮＧ）灌服，使大鼠胃粘膜出现癌前病变。给造模大鼠灌眼健胃灵口服液生，其胃粘膜细胞异型，结构异型的程度及血清癌胚抗原测定等指标均有好转。与生理盐水组比较，表明健胃灵口服液有较好的保护胃粘膜、阻断ＭＮＮＧ对胃粘膜损害的作用。     

幽门螺杆菌感染者胃粘膜癌前病变与Fas抗原表达的关系

目的 研究幽门螺杆菌(Hp)感染后胃粘膜癌前病变中Fas抗原表达的情况，了解Hp在胃癌发生过程中的作用．方法 采用免疫组织化学等方法捡测83例经病理证实为慢性胃炎病人胃粘膜上皮细胞中Fas抗原的表达情况。结果 在浅表性胃炎、萎缩性胃炎、肠化生及异型增生中，Fas抗原表达率分刷为20．00%、36．36％、73．33％、43．75%，Fas抗原在肠化生中的表达率显高于浅表性胃炎，萎缩性胃炎及异型增生(P<0．01及P相似文献   

血脂平口服液治疗高脂血症的临床实验研究

高脂血症是指血浆脂质浓度超过正常高限，若持继升高可引起心脑肾血管病变。近年来随着人们生活水平的提高，发病率大幅度上升。本研究据祖国医学理论辩证立方，自拟名为血脂平口服液，用于临床三年，并设烟酸肌醇脂作对照．现将完整资料撰成，报告如下。     

Calcitonin in human gastric mucosa and carcinoma

Summary The localization of immunoreactive calcitonin (IR-CT) in the human gastric mucosa and tumor tissues was studied using an immunohistochemical peroxidase-antiperoxidase method. A small number of IR-CT-containing cells were observed in both infant and adult gastric antral mucosa and the ratio of IR-CT-containing cells to G cells was about 1:50-100. Moreover, tissue content of IR-CT in normal antral mucosa was 2.37±0.35 ng/g wet weight. IR-CT-containing cells and G cells decreased with the progress of chronic atrophic gastritis and were totally absent in intestinal metaplastic glands. IR-CT was detected in G cells, suggesting a paracrine relation between gastrin and CT. IR-CT was not found in tumor cells of 35 gastric adenomas and 40 well differentiated adenocarcinomas. On the other hand, it was demonstrated in a very small number of tumor cells in 4 of 46 poorly differentiated adenocarcinomas, and in a good number in 3 of 7 scirrhous argyrophil cell carcinomas. IR-CT in plasma could serve, therefore, as a tumor marker of scirrhous endocrine cell carcinoma, and its production in cancer cells was considered to be eutopic rather than ectopic.Supported in part by Grant-in Aid for Cancer Research from the Ministry of Education, Science and Culture (No. 59010074).     

辣椒素对胃黏膜的作用及其机制

辣椒素(capsaicin)、辣椒素敏感传入神经元(CSAN)及辣椒素受体(VR1)与胃黏膜损伤及修复密切地相关.研究显示辣椒素对胃黏膜具有保护作用,可能与其增加胃黏膜血流、促进胃动力、调节胃酸和前列腺素分泌等因素有关.本文对辣椒素对胃黏膜的作用及其机制的研究进展进行综述.     

胃粘膜活检标本端粒酶和增殖细胞核抗原的表达意义

目的　探讨胃粘膜活检标本端粒酶和增殖细胞核抗原 (PCNA)的表达意义。方法　采用TRAP法及免疫组织化学方法对 12 2例胃粘膜活检标本端粒酶活性及PCNA表达情况进行同步检测。结果　胃癌组端粒酶阳性率为 88 1% (6 0 / 6 8) ,明显高于慢性浅表性胃炎组 (0 / 2 6 )及慢性萎缩性胃炎组 (4 / 18)。端粒酶表达阳性者胃癌组细胞增殖活性为 (82 9± 16 8) % ,明显高于端粒酶表达阴性组的 (6 3 8± 15 1) % (P <0 0 1)。端粒酶阳性率与PCNA计数值在胃癌组呈良好的协同性。结论　在胃粘膜活检标本中联合检测端粒酶及PCNA对阐明胃癌的发生发展 ,监测癌前病变及早期诊断胃癌有非常重要的意义     

Decreased expression of gastrokine 1 in gastric mucosa of gastric cancer patients

AIM: To investigate the expression of gastrokine 1(GKN1) in normal gastric mucosa, precancerous lesions and gastric cancer tissues, and to analyse its correlations with tumour site and pathological pattern.METHODS: Thirty gastric cancer patients(12 cases of diffuse type and 18 cases of intestinal type), 13 atrophic gastritis patients and 15 healthy volunteers with almost normal gastric mucosa(superficial gastritis) were enrolled in this study. Helicobacter pylori(H. pylori) infection was examined in all subjects. All gastric mucosa biopsy specimens were obtained. Cancer-adjacent specimens were taken from corresponding gastric cancer patients. Immunohistochemistry and real-time PCR were performed to determine the expressions of the GKN1 protein and m RNA, respectively.RESULTS: H. pylori infection had no significant association with age, gender, tumour site or pathological pattern in all subjects. Compared with the superficial gastritis and atrophic gastritis groups, the expression of GKN1 protein(P = 0.011) and m RNA(P < 0.001) in gastric cancer was significantly decreased. The GKN1 m RNA level in diffuse type gastric cancer was significantly lower than in intestinal type gastric cancer(0.296 ± 0.076 vs 0.525 ± 0.164, P < 0.001).CONCLUSION: Compared with almost normal gastric mucosa, GKN1 expression in the gastric mucosa of gastric cancer patients is decreased; this is associated with progression and prognosis of gastric cancer.     

肝硬化门脉高压大鼠胃粘膜屏障功能的观察

建立两组门脉高压症(PH)动物模型,并与对照组(SO组)比较,以观察胃粘膜屏障功能的改变.结果显示,与SO组比较,门静脉狭窄组(PVS组)大鼠内脏血流量明显增加(Ρ＜0.001),胃粘膜处于缺血状态,胃壁结合粘液(GP)显著下降(P＜0.01);肝硬化门脉高压症组(PL组)较PVS组降低更明显(P＜0.05);三组间胃基础泌酸量(BAS)无差异.PVS组、PL组大鼠H+返渗量(H+BD)均明显高于SO组(P＜0.001),表明PVS大鼠胃粘膜屏障功能破坏严重,尤以PL大鼠为甚;门脉高压胃病(PHG)与胃粘膜屏障功能严重减弱有关,其肝功能受损参与胃粘膜病变的发生.     

单独高胃泌素血症及其与MNNG合用时对大鼠胃黏膜的影响

目的探讨胃泌素及其与N-甲基-N’-硝基-N-亚硝基胍(MNNG)合用时对大鼠胃黏膜的影响。方法 Wistar大鼠共24只,分为4组:空白对照组(A组,6只):自由饮用纯净水;胃泌素组(G组,6只):连续每天皮下注射胃泌素300μg/kg共90d;MNNG组(M组,6只):饮用含MNNG100μg/ml的自来水;胃泌素与MNNG合用组(M+G组,6只):饮用含MNNG100μg/ml的自来水,同时连续注射胃泌素每天300μg/kg共90d。第90天取材,比较各组胃的组织学变化,并采用免疫组化的方法检测bcl-2(即B细胞淋巴瘤/白血病-2基因)和ki-67(增殖细胞核抗原)抗体在不同胃组织中的表达。结果 4个组动物胃黏膜层厚度差异有统计学意义(P<0.05),G组(964.70±170.5)μm及M+G组(817.68±109.5)μm与A组(785.43±98.3)μm和M组(713.50±92.3)μm相比,胃黏膜层增加。G组、M组及M+G组ki-67及bcl-2的表达量均增加,且与A组比较,差异均有统计学意义(P<0.05)。结论胃泌素能促进胃黏膜生长增厚且增加ki-67及bcl-2的表达量,提示单独高胃泌素血症有可能通过促进细胞增殖及抑制细胞凋亡而最终导致肿瘤的形成。     

丙型肝炎患者胃粘膜细胞中HCV NS3、NS5抗原定位研究

目的了解丙型肝炎病毒（ＨＣＶ）在胃粘膜细胞中的感染状况。方法采用免疫组化Ｓ－Ｐ法以抗－ＨＣＶＮＳ３和抗－ＨＣＶＮＳ５检测了３３例慢性丙型肝炎（ＣＨＣ）患者胃粘膜组织中ＨＣＶＮＳ３及ＨＣＶＮＳ５抗原。结果ＨＣＶＮＳ３、ＨＣＶＮＳ５的检出率分别为３３．３３％（１１／３３）和２７．２７％（９／３３）。阳性物质沉积于胃粘膜上皮腺体细胞胞浆，呈单个或片灶状分布，部分病例淋巴细胞胞浆中也可见到。结论胃粘膜细胞中ＨＣＶ抗原的检出，表明ＨＣＶ可能是一种泛嗜性病毒，其机制及生物学意义须进一步研究。     

氨基己糖和磷脂含量评价胃粘膜防御机制

目的测定大鼠胃粘膜氨基己糖和磷脂含量，以证实其对评价胃粘膜防御机制的价值．方法大鼠３６只分为５个组．Ⅰ组为正常对照组；Ⅱ组为无水乙醇损伤对照组；Ⅲ组为胶体次枸橼酸铋加无水乙醇组；Ⅳ组为硫糖铝加无水乙醇组；Ⅴ组为雷尼替丁加无水乙醇组．测定胃粘膜氨基己糖、磷脂含量以及胃粘膜血流（ＧＭＢＦ）、跨胃粘膜电位（ＰＤ）及胃粘膜表面疏水性．结果乙醇损伤大鼠胃粘膜后，氨基己糖（ｍｇ／ｇ蛋白：０２１±００３ｖｓ０５９±００４）和磷脂含量（ｍｇ／ｇ蛋白：０３２±００４ｖｓ０６４±００４）显著下降（Ｐ＜００１）．胶体次枸橼酸铋和硫糖铝可使氨基己糖及磷脂较乙醇损伤对照组显著升高（Ｐ＜００１）．胃粘膜氨基己糖及磷脂含量与ＧＭＢＦ，ＰＤ和粘膜表面疏水性呈显著正相关．结论胃粘膜氨基己糖及磷脂含量对评价胃粘膜损伤和药物疗效有重要价值．     

Non-invasive diagnosis of gastric mucosal atrophy in an asymptomatic population with high prevalence of gastric cancer

AIM: To validate a non-invasive method to detect gastric mucosal atrophy in a Chilean population with high prevalence of gastric cancer and a poor survival rate. METHODS: We first determined the optimal cut-off level of serum pepsinogen (PG)-1, PG-1/PG-2 ratio and 17-gastrin in 31 voluntary symptomatic patients (mean age: 66.1 years), of them 61% had histologically confirmed gastric atrophy. Then, in a population-based sample of 536 healthy individuals (209 residents in counties with higher relative risk and 327 residents in counties with lower relative risk for gastric cancer), we measured serum anti-H pylori antibodies, PG and 17-gastrin and estimated their risk of gastric cancer. RESULTS: We found that serum PG-1 < 61.5 microg/L, PG-1/PG-2 ratio < 2.2 and 17-gastrin > 13.3 pmol/L had a high specificity (91%-100%) and a fair sensitivity (56%-78%) to detect corpus-predominant atrophy. Based on low serum PG-1 and PG-1/PG-2 ratio together as diagnostic criteria, 12.5% of the asymptomatic subjects had corpus-predominant atrophy (0% of those under 25 years and 20.2% over 65 years old). The frequency of gastric atrophy was similar (12% vs 13%) but H pylori infection rate was slightly higher (77% vs 71%) in the high-risk compared to the low-risk counties. Based on their estimated gastric cancer risk, individuals were classified as: low-risk group (no H pylori infection and no atrophy; n = 115; 21.4%); moderate-risk group (H pylori infection but no atrophy; n = 354, 66.0%); and high-risk group (gastric atrophy, with or without H pylori infection; n = 67, 12.5%). The high-risk group was significantly older (mean age: 61.9+/-13.3 years), more frequently men and less educated as compared with the low-risk group. CONCLUSION: We propose to concentrate on an upper gastrointestinal endoscopy for detection of early gastric cancer in the high-risk group. This intervention model could improve the poor prognosis of gastric cancer in Chile.     

乙型肝炎患者胃粘膜中HBsAg表达分析

目的探讨乙型肝炎病毒的泛嗜性.方法采用免疫组化SP法,对45例随机采样的乙型肝炎患者行胃粘膜活检,作HBsAg检测;采用ELISA法检测静脉血HBV.结果乙型肝炎患者胃粘膜中有HBsAg存在,主要呈弥漫胞浆型分布,腺腔和胃小凹及间质中也可见HBsAg颗粒;血清中HBV单项或多项阳性者,胃粘膜HBsAg阳性率相近(75～80%);各类型乙型肝炎患者胃粘膜HBsAg阳性率比较CAH＞CPH＞HLC,但彼此之间差异不显著(P＞0.005).结论乙肝患者胃粘膜中有HBsAg存在.