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1.
对30例肾病综合症(Ns)患儿与10例正常健康儿童所进行的CD系列阳性细胞与免疫球蛋白调节改变的相关性进行对照研究。按全国统一诊断标准,诊断为单纯性综合症18例(B组);肾炎性肾病综合症12例(C组);健康儿童对照组10例(A组)。B组平均年龄6.6岁,C组7.1岁,A组6.1岁。B组男女各9例,C组男性8例、女性4例,A组男性4例、女性6例。几种CD+细胞检测结果见表:从CD4+/CD8+的比值与IgG含量的关系看:单纯性NS组患儿CD4+/CD8+细胞比值与血清IgG含量水平无相关性(r=0… 相似文献
2.
本实验利用大鼠坐骨神经钳夹损伤模型,观察了电针(A组)、直流刺激器(B组)及双向平衡电脉冲刺激器(C组)对神经损伤后自残及神经再生的影响,D组为对照。结果:(1)术后17d,C组的右后足趾自残率为13%,而A、B及D组分别为46%、53%及80%,C组的自残率显著低于其它各组(P<0.001);(2)右侧屈肌反射阈:当刺激强度为35±5V(C组)、45±5V(A和B组)时,可引出短潜伏期(45~150ms)的A类及长潜伏期(125~525ms)C类传入纤维反应,而当刺激强度增至65±5V时,于D组仅见长潜伏期(190~600ms)的C类传入纤维反应;(3)术后17d脊神经节的标记细胞百分享分别为11.1%(C组)、5.7%(B组)、5.2%(A组)及1.1%(D组);脊髓前角的标记细胞百分率分别为16.6%(C组)、8.1%(8组)、7.4%(A组)及1.9%(D组)。上述结果表明:双向平衡电脉冲刺激器在抑制神经损伤后自残及促进周围神经再生等方面具有更显著的生理功效。 相似文献
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病毒性心肌炎、扩张型心肌病患者T细胞及其亚群和免疫球蛋白的改变 总被引:1,自引:0,他引:1
本研究用CD系统MCAB间接免疫荧光法对柯萨奇B病毒中和抗体阳性的病毒性心肌炎(B型),扩张型心肌病(C组)患者检测其周围T淋巴细胞及其亚群并与正常人(A组)相对照,结果不论是B组或C组其CD3、CD4、CD3均较A组为低,P值均<0.01,C组比B组更低,两者相比亦有统计学意义,P值分别为<0.05,<0.001。至于CD4/CD8,C组>B组>A组,P值为<0.05,<0.01。在急性恢复期者与正常人相近,分别为1.78±0.05、1.73±0.08,而慢性反复发作期者与扩张型心肌病者相近,分别为2.22±0.3及2.29±0.26。免疫球蛋白测定在扩张型心肌病中较在病毒性心肌炎息者明显增高,并有统计学意义 相似文献
4.
哮喘患者支气管肺泡灌洗液中细胞分类及其T细胞亚群的改变 总被引:3,自引:0,他引:3
目的和方法:采用间接免疫荧光法和支气管肺泡灌洗液(bronchialalveolarlavagefluid,BALF)技术观察12例过敏性哮喘患者和23例健康人外周血和BALF中的T淋巴细胞亚群变化:结果:与健康对照组比较,过敏性哮喘患者外周血的T淋巴细胞亚群无明显改变,但其BALF中的CD4+细胞显著增高(54.97±414)%vs(79.71±9.63)%,P<005;CD4+与CD8+细胞的比值也显著增高(164±0.32vs2.32±0.83,P<005)。此外,过敏性哮喘患者BALF中肥大细胞和嗜酸细胞百分比(009±0.04)%和(362±1.06)%明显高于健康对照组(002±0.01)%和(0.39±0.30)%,P<005和P<001。结论:CD4+细胞在哮喘的气道炎症中发挥重要作用。 相似文献
5.
本文报道用流式细胞分析技术,检测了28例多发性骨髓瘤(MM)患者和20名健康对照者外周血B细胞(CD20+)以及B、T和单核细胞中HLA-DR+细胞比率。结果发现,MM患者CD20+以及B、T及单核细胞中HLA-DR+细胞比率与正常对照组差异非常显著(P<0.01)。加入重组IL-4,可使8名MM患者CD20+、HLA-DR+CD20+、HLA-DR+单核细胞比率提高非常显著(P<0.01)。而在T细胞,P值则>0.05。我们认为由于IL-4分泌减少,一方面使多克隆B细胞激活及增殖受抑,另一方面使单核细胞HLA-DR抗原表达减少,抗原提呈能力下降可能是MM发生多克隆免疫球蛋白抑制的重要原因。 相似文献
6.
慢性脑缺血对青年和老年大鼠海马NOS阳性神经元的影响 总被引:4,自引:0,他引:4
本文利用组织化学方法观察了慢性脑缺血对青、老年大鼠海马NOS阳性神经元的影响。慢性脑缺血采用双侧颈总动脉永久性结扎模型。结果:青年缺血1 月组大鼠海马CA1、CA2~3 和DG 亚区NOS阳性神经元面数密度分别为29.1±2.3、23.5±2.1 和39.3±2.8,小于对照组相应三个亚区(49.6±1.3、49.3±2.1 和64.7±2.1),P< 0.01;青年缺血3 月组大鼠CA1、CA2~3 和DG 亚区NOS阳性神经元面数密度分别为40.2±1.6、39.3±2.5 和48.4±1.8,和对照组者相近,但大于缺血1 月组(P< 0.01)。老年缺血1 月组大鼠海马各区NOS阳性神经元面数密度分别为39.6±1.5、35.6±2.1 和54.7±2.5,小于老年对照组相应三个亚区(55.8±1.7、51.3±1.7 和64.9±1.9),P< 0.01;老年缺血3 月组大鼠各亚区NOS阳性神经元面数密度分别为43.1±2.4、38.7±3.4 和54.7±3.2,仍然小于对照组,P< 0.01。结果提示:慢性脑缺血可以影响大鼠海马NOS阳性神经元,但青年和老年大鼠海马NOS神经元对慢性脑缺血损伤的易感性和反应性不同。 相似文献
7.
T细胞表面6种细胞表型的变化与大肠癌分期及术式的关系 总被引:8,自引:0,他引:8
应用流式细胞仪检测39例大肠癌患者手术前后T细胞表面6种细胞表型,发现随着Dukes分期的增高,术前CD3、CD4、CD4/CD8、CD16、CD69及CD3+/HLA-DR+逐渐下降,CD8逐渐增高(P<0.05);A期大肠癌患者机体免疫功能活跃,术前CD3、CD4高于对照组(P<0.05),CD8、CD4/CD8、CD16、CD3+/HLA-DR+与对照组相同;根治及姑息性切除术后,CD8降低,CD3、CD4、CD4/CD8、CD16、CD69、CD3+/HLA-DR+升高(P<0.05);肿瘤未切组术后CD3、CD16、CD4/CD8进一步下降(P<0.05)。提示大肠癌患者术前免疫状态与疾病的程度呈负相关,切除肿瘤有益于改善患者细胞免疫功能。 相似文献
8.
雌二醇增强大鼠空间记忆能力的研究 总被引:7,自引:1,他引:6
目的:探讨雌激素对学习记忆能力的影响。方法:3月龄雌性 SD大鼠 28只,体重 200 g,分用药组(雌二醇肌注0.25 mg/kg/日)和对照组各14只,每组有9只动物用Moms水迷宫技术测试大鼠学习记忆能力,另5只在用药 10天后用 Golgi-Cox染色,观察海马 CAI区锥体细胞树突棘的变化。结果:(1)用药 10天后,定位航行实验显示:后 4个时间段用药组和对照组潜伏期分别为 8. 6±3.1秒和 14.1±4.2秒,差别显著(P<0.05);Golgi-Cox染色显示对照组中段树突棘数为 19.5±2. 3,用药组为 24.8±3.2,差别显著(P<0.05)。(2)用药 30天后,用药组和对照组潜伏期分别为17.4±6.2秒和35.9±9.3秒,在原有平台像限游泳路线长度占总长度42.3±4.3%和31.7±4.6%;跨越平台次数分别为3.1次和1.6次,差别显著(P<0.05)。结论:雌二醇能增强大鼠学习记忆能力,特别是记忆能力。 相似文献
9.
香菇多糖冲剂对反复呼吸道感染患儿免疫功能的影响 总被引:4,自引:0,他引:4
本文报道应用香菇多糖冲剂治疗反复呼吸道感染患儿60例。观察治疗前后临床症状、体液免疫和细胞免疫变化。结果显示治疗后血清Ig和C3水平均比治疗前提高,尤以IgA和C3为明显(P<0.05),CD3+细胞和CD4+细胞百分率显著增加(P<0.01),CD8+细胞百分率无变化,CD4+/CD8+细胞比值有所上升。活化的淋巴细胞表面白细胞介素2受体(Tac)的表达率明显增高(P<0.001)。临床观察总有效率达96.7%。提示香菇多糖冲剂对反复呼吸道感染患儿有广泛的免疫刺激作用。该冲剂疗效好,服用方便,未发现任何毒副作用。 相似文献
10.
为探讨丙型肝炎(HC)病人细胞免疫功能和丙型肝炎病毒(HCV)的致病机制及机体对其免疫保护作用,收集24例HC病人(急性3例,慢性21例),用3H-TdR掺入法研究病人外周血单个核细胞(PBMC)对不同HCV抗原增殖反应,并用流式细胞仪(FACS)检测了PBMC中CD4+、CD8+淋巴细胞亚群在HCV抗原刺激后的变化。结果:HC病人PBMC对HCV合成肽CP9,NS4和基因重组抗原C,E1,E2,NS3刺激后出现不同程度增殖反应,刺激指数(SI)分别为1.69±0.51,1.61±0.54,1.68±0.58,1.49±0.44,1.44±0.44和1.33±0.33。3例急性HC中2例病人的PBMC对HCV抗原呈有效增殖反应(SI≥2.1),且血清HCVRNA阴转伴ALT正常。细胞表型分析显示:增殖的细胞表型是CD4+淋巴细胞,而CD8+淋巴细胞增殖反应较弱。结论:HC病人PBMC确实存在对HCV抗原的增殖反应;CD4+淋巴细胞比CD8+淋巴细胞增殖反应要强,急性HC病人PBMC对HCV抗原有效的增殖反应预示可能有良好的临床愈合 相似文献
11.
OBJECTIVE: The purpose of this article is to review the role of behavioral research in disease prevention and control, with a particular emphasis on lifestyle- and behavior-related cancer and chronic disease risk factors--specifically, relationships among diet and nutrition and weight and physical activity with adult cancer, and tracking developmental origins of these health-promoting and health-compromising behaviors from childhood into adulthood. METHOD: After reviewing the background of the field of cancer prevention and control and establishing plausibility for the role of child health behavior in adult cancer risk, studies selected from the pediatric published literature are reviewed. Articles were retrieved, selected, and summarized to illustrate that results from separate but related fields of study are combinable to yield insights into the prevention and control of cancer and other chronic diseases in adulthood through the conduct of nonintervention and intervention research with children in clinical, public health, and other contexts. RESULTS: As illustrated by the evidence presented in this review, there are numerous reasons (biological, psychological, and social), opportunities (school and community, health care, and family settings), and approaches (nonintervention and intervention) to understand and impact behavior change in children's diet and nutrition and weight and physical activity. CONCLUSIONS: Further development and evaluation of behavioral science intervention protocols conducted with children are necessary to understand the efficacy of these approaches and their public health impact on proximal and distal cancer, cancer-related, and chronic disease outcomes before diffusion. It is clear that more attention should be paid to early life and early developmental phases in cancer prevention. 相似文献
12.
Riann M. Palmieri-Smith Mark Villwock Brian Downie Garin Hecht Ron Zernicke 《Journal of Athletic Training》2013,48(2):186-191
Context:
Quadriceps dysfunction is a common consequence of knee joint injury and disease, yet its causes remain elusive.Objective:
To determine the effects of pain on quadriceps strength and activation and to learn if simultaneous pain and knee joint effusion affect the magnitude of quadriceps dysfunction.Design:
Crossover study.Setting:
University research laboratory.Patients or Other Participants:
Fourteen (8 men, 6 women; age = 23.6 ± 4.8 years, height = 170.3 ± 9.16 cm, mass = 72.9 ± 11.84 kg) healthy volunteers.Intervention(s):
All participants were tested under 4 randomized conditions: normal knee, effused knee, painful knee, and effused and painful knee.Main Outcome Measure(s):
Quadriceps strength (Nm/kg) and activation (central activation ratio) were assessed after each condition was induced.Results:
Quadriceps strength and activation were highest under the normal knee condition and differed from the 3 experimental knee conditions (P < .05). No differences were noted among the 3 experimental knee conditions for either variable (P > .05).Conclusions:
Both pain and effusion led to quadriceps dysfunction, but the interaction of the 2 stimuli did not increase the magnitude of the strength or activation deficits. Therefore, pain and effusion can be considered equally potent in eliciting quadriceps inhibition. Given that pain and effusion accompany numerous knee conditions, the prevalence of quadriceps dysfunction is likely high.Key Words: arthrogenic muscle inhibition, central activation failure, voluntary activation, musclesKey Points
- Knee pain and effusion resulted in arthrogenic muscle inhibition and weakness of the quadriceps.
- The simultaneous presence of pain and effusion did not increase the magnitude of quadriceps dysfunction.
- To reduce arthrogenic muscle inhibition and improve muscle strength, clinicians should employ interventions that target removing both pain and effusion.
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《Expert Review of Clinical Immunology》2013,9(3):323-331
Autoimmunity is still a mystery of clinical immunology and medicine as a whole. The etiology and pathogenesis of autoimmune disorders remain unclear and, thus, are assessed as a balance between hereditary predisposition, triggering factors and the appearance of autoantibodies and/or self-reactive T cells. Among the immunological armamentarium, molecular mimicry, based on self-reactive T- and B-cell activation by cross-reactive epitopes of infectious agents, is of special value. Hypotheses regarding the possible involvement of molecular mimicry in the development of postinfectious autoimmunity are currently very intriguing. They provide new approaches for identifying etiological agents that are associated with postinfectious autoimmunity, paired microbial- and tissue-linked epitopes targeted for autoimmune reaction determination, postinfectious autoimmunity pathogenesis recognition and specific prevention, and therapy for autoimmune disorder development. 相似文献
15.
Although drugs of abuse have different acute mechanisms of action, their brain pathways of reward exhibit common functional effects upon both acute and chronic administration. Long known for its analgesic effect, the opioid beta-endorphin is now shown to induce euphoria, and to have rewarding and reinforcing properties. In this review, we will summarize the present neurobiological and behavioral evidences that support involvement of beta-endorphin in drug-induced reward and reinforcement. Currently, evidence supports a prominent role for beta-endorphin in the reward pathways of cocaine and alcohol. The existing information indicating the importance of beta-endorphin neurotransmission in mediating the reward pathways of nicotine and THC, is thus far circumstantial. The studies described herein employed diverse techniques, such as biochemical measurements of beta-endorphin in various brain sites and plasma, and behavioral measurements, conducted following elimination (via administration of anti-beta-endorphin antibodies or using mutant mice) or augmentation (by intracerebral administration) of beta-endorphin. We suggest that the reward pathways for different addictive drugs converge to a common pathway in which beta-endorphin is a modulating element. beta-Endorphin is involved also with distress. However, reviewing the data collected so far implies a discrete role, beyond that of a stress response, for beta-endorphin in mediating the substance of abuse reward pathway. This may occur via interacting with the mesolimbic dopaminergic system and also by its interesting effects on learning and memory. The functional meaning of beta-endorphin in the process of drug-seeking behavior is discussed. 相似文献
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PTEN与信号转导及肿瘤 总被引:3,自引:2,他引:3
TEN[1] (phosphataseandtensinhomologydeletedonchromosometen)又名MMAC1 [2 ] (mutatedinmutiplyadancedcancer 1 )和TEP1 [3 ] (TGF -βregulatedandepithelialcell -richedphosphatase 1 ) (以下均称为PTEN) ,是 1 997年由 3个研究小组先后发现的一个具有双特异磷酸酶活性的抑癌基因。PTEN基因异常广泛存在于人类多种恶性肿瘤 ,如恶性神经胶质瘤、前列腺癌、子宫内膜癌、黑色素瘤等… 相似文献
18.
Tobacco and alcohol and the risk of head and neck cancer 总被引:2,自引:0,他引:2
H. Maier A. Dietz U. Gewelke W. D. Heller H. Weidauer 《Journal of molecular medicine (Berlin, Germany)》1992,70(3-4):320-327
Summary We carried out two case-control studies on the relative risk of head and neck cancer in association with tobacco and alcohol consumption. The first study carried out at the ENT Department of the University hospitals of Heidelberg and Giessen (FRG) comprised 200 male patients with squamous cell cancer of the head and neck and 800 control subjects matched for sex, age, and residential area (1:4 matching design). Of the tumour patients, 4.5% had never smoked, in contrast to 29.5% of the control group. The average tobacco and alcohol consumption of the patients was approximately twice as high as in the control subjects. The highest alcohol and tobacco consumption was observed in patients suffering from oropharyngeal cancer. Tobacco and alcohol increased the risk of head and neck cancer in a dose-dependent fashion and acted as independent risk factors. In heavy smokers (> 60 pack-years) a relative risk of 23.4 (alcohol adjusted) was calculated. Combined alcohol and tobacco consumption showed a synergistic effect. The risk ratio increased more in a multiplicative than in an additive manner. Oral and laryngeal cancer were associated with the highest tobacco-associated risk values. The highest ethanol-associated risk values were associated with oropharyngeal and laryngeal cancer. The second study was carried out at the ENT Department of the University of Heidelberg on 164 males with squamous cell carcinoma of the larynx and 656 control subjects matched for sex, age and residential area (1:4 matching design). Of the cases, 4.2% had never smoked, compared with 28.5% of the control subjects. The risk of laryngeal cancer by tobacco consumption was dose dependent, reaching a maximum value of 9.1 (adjusted for alcohol) for a consumption of more than 50 tobacco-years (TY). The relative risk of laryngeal cancer associated with alcohol intake was also dose dependent, reaching a value of 9.0 (adjusted for tobacco) for a mean daily consumption of more than 75 g alcohol. An analysis of subsite specific risks showed that heavy smokers (> 50 TY) carried a nearly ten times higher risk of supraglottic cancer than of glottic cancer. The risk of supraglottic cancer from alcohol consumption was also higher than that of glottic cancer. 相似文献
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类赖氨酰氧化酶2(lysyl oxidase-like 2,LOXL2)是赖氨酰氧化酶(lysyl oxidase,LOX)基因家族的成员之一,其表达产物能促进胶原沉积.LOXL2的过表达能促进纤维化,并与肿瘤侵袭、转移及不良预后有关.目前大部分学者认为LOXL2是一种转移促进基因,也有实验支持其是一种肿瘤抑制基因.研究发现LOXL2可以通过激活Snail/Ecadherin通路或Src/FAK通路促进转移.LOXL2有望作为肿瘤生物标志物,用于预后判断,成为一个新的治疗靶点. 相似文献
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Forty healthy males (M) and females (F) divided into two different age groups i.e. M50 years (range 44–57; n= 9), F50 years (range 43–54; n= 9), M70 years (range 64–73; n= 11) and F70 years (range 63–73; n= 11) volunteered as subjects for examination of muscle cross-sectional area (CSA) and maximal voluntary isometric force production characteristics of the leg extensor muscles and serum androgen and sex hormone binding globulin (SHBG) concentrations. The CSA in the male groups was greatly larger (P < 0.01) than in the female groups and both elderly groups demonstrated slightly (n.s.) smaller values in the CSA than the two middle-aged groups. Maximal force of 2854 ± 452 N in M50 was greater (P < 0.05) than that of 2627 ± 752 N recorded for F50 as well as the force of 2787 ± 843 in M70 was greater (P < 0.001) than that of 1849 ± 295 recorded for F70. The force between F50 and F70 differed significantly (P < 0.05) from each other. The maximal rate of force production in M50 was greater (P < 0.01) than in F50 as well as in M70 greater (P < 0.001) than in F70. Both middle-aged groups demonstrated greater (P < 0.05) values than the respective elderly groups of the same sex. The individual values in the CSA correlated with the values in maximal force both in the middle-aged subjects (r= 0.66; P < 0.01) and in the elderly subjects (r= 0.69; P < 0.01). The mean concentration of serum testosterone in M50 was slightly (n.s.) greater than in M70 and in F50 significantly (P < 0.05) greater than in F70. Serum SHBG levels were lower in the males (P < 0.01) than in the females and serum testosterone/SHBG ratio in M70 and in F70 were lower (P < 0.05) than in M50 and in F50, respectively. In the females significant positive correlations were observed between the individual values in serum testosterone concentration and the values both in the CSA (r= 0.46; P < 0.05) and in maximal force (r= 0.62; P < 0.01) as well as between serum testosterone/SHBG ratio and both the CSA (r= 0.55; P < 0.05) and maximal force (r= 0.68; P < 0.01). The present results imply that the decreasing basal level of blood testosterone over the years in aging people, especially in females, may lead to decreasing anabolic effects on muscles thus having an association with age-related declines in the maximal voluntary neuromuscular performance capacity in aging people. 相似文献