MMP2及TIMP2的表达与食管癌淋巴结转移的关系

目的探讨食管癌组织中金属基质蛋白酶-2（MMP2）及其抑制因子-2（TIMP2）的表达与淋巴结转移的关系及意义。方法采用免疫组化检测100例食管癌组织中MMP-2与TIMP-2的基因表达情况,分析MMP-2及TIMP-2表达与食管癌淋巴结转移的关系。结果食管癌组织中MMP-2及TIMP2在淋巴结转移病例和无淋巴结转移病例的表达率分别为：87.69%、64.62%和68.57%,85.71%。提示：MMP-2的阳性表达率与食管癌的淋巴结转移有统计学意义（x2=5.40,P〈0.05）;TIMP-2的阳性表达率与食管癌淋巴结转移有统计学意义（x2=5.02,P〈0.05）。结论 MMP-2、TIMP-2的阳性表达与食管癌的淋巴结转移有关,并有助于确定术后治疗方案。     

成批烧伤病人的救治体会

成批烧伤常突然发生 ,伤员多 ,伤情复杂。对成批烧伤病人的救治是否及时正确 ,直接影响到患者的生命安全和机体功能的恢复。我科从 1 990 - 0 1～2 0 0 2 - 1 2共收治 2 4批 1 2 6例成批烧伤病人 ,现结合有关临床资料对成批烧伤的救治 ,讨论如下。1　临床资料本组共 2 4批 1 6 2例 ,其中男性 6 4例 ,女性 98例。平均年龄 (32± 1 1 2 )岁。每批人数 4～ 2 8例不等。其中 ,火焰烧伤 1 2批 78例 ;火药爆炸伤 8批 6 2例 ;化学灼伤 2批 1 0例 ;其他原因烧伤 2批 1 0例。平均烧伤面积 2 0 1 %～ 4 5 2 %。平均Ⅲ度烧伤面积30 0 2 %～ 1 6 4 …     

前列腺素代谢对脑血管痉挛的反应

本实验用放射免疫法研究了血管痉挛后PGI_2、TXA_2、PGE_2的变化。急性痉挛,动脉壁及血浆中的PGI_2、TXA_2、PGE_2均无变化。慢性痉挛,动脉壁PGI_2生成明显减少(P<0.01),而TXA_2、PGE_2及血浆中PGI_2、TXA_2、PGE_2无明显变化。血凝块孵育后,PGE_2 6h 开始减少,24h 几乎接近消失。本实验提示:动脉壁PGI_2生成的减少,在慢性痉挛中起着重要作用。     

IMP2基因的生物学特性及其与疾病关系的研究进展

<正>胰岛素样生长因子-2 mRNA结合蛋白2(Insulin-like growth factor 2 mRNA-binding protein 2,IGF2bP2,IMP2)隶属于IGF-2 mRNA结合蛋白家族成员(IGF2bPs, IMPs),IMPs家族包括三类成员：IMP1、IMP2和IMP3。有研究发现,除了IGF2 mRNA,IMP2还可与其他mRNA结合,如laminin-β2、PINCH2、MURF3等,     

硝苯地平对过氧化氢引起的心肌纤维化的抑制作用及机制

目的研究硝苯地平(nifedipine)对过氧化氢(H2O2)引起的心肌纤维化的影响及机制。方法原代培养大鼠心脏成纤维细胞(CFs),用100μmol/L H2O2刺激,蛋白免疫印迹法(Western blot)检测硝苯地平(10μmol/L)以及对照药维拉帕米(verapamil,10μmol/L)对H2O2诱导的结缔组织生长因子(CTGF)、纤维粘连蛋白(FN)的蛋白表达以及信号蛋白MAPK磷酸化的影响;利用fluo-4/AM钙荧光探针法测定硝苯地平对H2O2刺激引起CFs内钙离子浓度([Ca2+]i)变化的影响。结果 (1)硝苯地平能够显著抑制H2O2诱导的CTGF、FN蛋白表达上调以及细胞外信号调控蛋白激酶1/2(ERK1/2)、c-Jun N端激酶(JNK)磷酸化,而对p38MAPK磷酸化没有影响,维拉帕米对以上蛋白表达均无抑制作用;(2)硝苯地平对H2O2刺激引起的CFs中的[Ca2+]i增加没有影响;(3)ERK信号通路抑制剂PD98059(10μmol/L)可抑制H2O2诱导的CTGF、FN的蛋白表达上调。结论硝苯地平可抑制H2O2刺激引起的CTGF、FN蛋白表达上调,而对[Ca2+]i变化没有影响,其抗纤维化的作用不依赖于经典的钙离子阻断作用,可能与抑制ERK1/2磷酸化有关。     

定量检测CCL20的竞争性内参照RT-PCR的建立及其初步应用

目的　建立检测趋化因子CC亚家族配体2 0 (CCL2 0 )表达水平的内参照定量逆转录聚合酶链反应(RT PCR)方法。方法　将人CCL2 0编码基因克隆到pBs ΔANK质粒中(PAL 2 ) ,将一4 2bp的外源核苷酸片断插入到上述重组质粒的CCL2 0中间,构建内参照质粒PAL- 2 - IS。在该定量系统中以PAL- 2 -IS和PAL- 2为模板,用CCL2 0的特异性引物扩增,根据扩增出的两个不同大小的条带的光密度扫描值对CCL2 0进行相对定量。结果　将PAL -2与已知不同系列浓度的PAL- 2 - IS以内参照竞争定量RT- PCR共扩增定量PAL- 2浓度,PAL- 2计算浓度与实际浓度无差异(P >0 .0 5 )。在同一细胞数量水平(10 5数量级) ,内参照竞争定量RT- PCR检测表明:CCL2 0在L0 2、HepG2、Hepal、Hepa3中表达水平高于Hepa2 ,而在HepG2 .2 .15中最低。结论　建立了检测CCL2 0表达水平的内参照定量RT -PCR方法,该方法可用于细胞中CCL2 0不同表达水平的检测。     

青葙皂苷对过氧化氢诱导的H9c2心肌细胞凋亡的影响及机制研究

目的 研究青葙皂苷（Celosins,CES）对H2O2诱导的H9c2凋亡的保护作用。方法 噻唑蓝检测各组细胞活力以确定CES的最佳作用浓度,以及对H2O2诱导H9c2心肌细胞损伤的保护作用。细胞分组为：空白对照组、H2O2处理组、低剂量青葙皂苷+H2O2处理组、中剂量青葙皂苷+H2O2处理组、高剂量青葙皂苷+H2O2处理组。利用流式细胞术检测心肌细胞凋亡比例、ROS的产生情况。蛋白印记法（WB）检测凋亡信号通路相关蛋白的表达和Nrf2 /ARE 信号通路相关蛋白表达。结果 （1）青葙皂苷可升高H2O2处理的H9c2细胞活力,差异具有统计学意义(P＜0.05)。（2）青葙皂苷可降低H2O2处理的H9c2细胞凋亡率(P＜0.05)。降低ROS的产生(P＜0.05)。（3）青葙皂苷可降低蛋白细胞质细胞色素C、cleaved-caspase-9、cleaved-caspase-3、Bax的表达(P＜0.05),升高蛋白Bcl-2、Nrf2 核转位与HO-1的表达(P＜0.05)。结论 CES通过激活Nrf2 /ARE信号通路抑制H2O2诱导的H9c2细胞损伤。     

雌二醇对大鼠垂体前叶细胞增殖的影响及其机制的初步探讨

观察了17-β羟雌二醇（17-β-estradiol，E2）及其代谢产物2-羟雌酮（2-hydroxyestrone，2－OHE1）、2-羟雌二醇（2－hydroxyestradiol，2－OHE2）和多巴胺（dopamine，DA）受体激动剂piribedil对大鼠垂体前叶细胞（aneriorpituitarycells，APC）增殖的影响。结果表明：E2（10－6mol/L）可促进APC生长，A10－5mol/L的piribedil却可抑制APC的增殖活性。提前12h加入E2或2－OHE2、2－OHE2可抑制piribedil的效应，但E2对piribedil效应无明显影响。     

MMP-2、TIMP-2的表达与骨巨细胞瘤复发的相关性

目的研究骨巨细胞瘤组织中基质金属蛋白酶-2(Matrix metalloproteinase-2)及其特异性抑制剂金属蛋白酶组织抑制因子-2(Tissue inhibitor of metalloproteinase-2)的表达与肿瘤血管形成和关系。方法用免疫组化SP法检测45例骨巨细胞瘤组织中MMP-2、TIMP-2的表达情况,以CD31标记检测微血管密度(MVD),计算肿瘤复发组和非复发组MMP-2/TIMP-2的比值,分析这些指标与骨巨细胞瘤预后的关系。结果骨巨细胞瘤组织有高水平的MMP-2、TIMP-2和CD31表达。复发组表达MMP-2、MMP-2/TIMP-2比值以及MVD均显著高于非复发组,但与组织学分级无关。MMP-2与MVD呈正的直线相关。结论MMP-2/TIMP-2比值升高与骨巨细胞瘤的复发以及肿瘤血管形成有关。     

金雀异黄素对氧化损伤的人晶状体上皮细胞凋亡的防护作用

目的：探讨金雀异黄素（Gen）对氧化损伤的人晶状体上皮细胞（HLEC）凋亡有无抑制作用。方法：本研究在终浓度3×10^-4mol/L H2O2培养液中复制HLEC凋亡模型,并以雌二醇（E2）作为阳性对照,采用流式细胞仪（FCM）检测HLEC凋亡率和线粒体膜电位（ΔΨM）的变化。结果：FCM结果显示：H2O2组HLEC凋亡率显著高于空白对照组;E2和Gen组凋亡率均低于H2O2组（P〈0.01）。H2O2组线粒体膜电位比对照组显著下降;E2组和Gen组的线粒体膜电位均比H2O2组升高（P〈0.01）。结论：Gen可减轻实验性氧化损伤的HLEC凋亡程度。     

R-CHOP方案治疗老年弥漫大B细胞性淋巴瘤的长期研究结果分析——GELA的一项临床试验

1 文献类型 治疗。 2 证据水平 1b。     

MiR-503 regulates cisplatin resistance of human gastric cancer cell lines by targeting IGF1R and BCL2

Background Studies have shown that the drug resistance of gastric cancer cells can be modulated by abnormal expression of microRNAs （miRNAs）.We investigated the role of miR-503 in the development of cisplatin resistance in human gastric cancer cell lines.Methods MiR-503 expression was measured by quantitative real-time PCR.MTT （3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide） and clonogenic assays were used to examine changes in cell viability and the drug resistance phenotype of cancer cells associated with upregulation or downregulation of the miRNA.A dual-luciferase activity assay was used to verify target genes of miR-503.Immunohistochemistry,Western blotting analysis,and a flow cytometric apoptosis assay were used to elucidate the mechanism by which miR-503 modulates drug resistance in cancer cells.Results MiR-503 was significantly downregulated in gastric cancer tissues and several gastric cancer cell lines.Additionally,downregulation of miR-503 in the cisplatin （DDP）-resistant gastric cancer cell line SGC7901/DDP was concurrent with the upregulation of insulin-like growth factor-1 receptor （IGF1R） and B-cell lymphoma 2 （BCL2） expression compared with the parental SGC7901 cell line.An in vitro drug sensitivity assay showed that overexpression of miR-503 sensitized SGC7901/DDP cells to cisplatin.The luciferase activity of reporters driven by IGF1R and BCL2 3＇-untranslated regions in SGC7901/DDP cells suggested that IGF1R and BCL2 were both direct target genes of miR-503.Enforced miR-503 expression in SGC7901/DDP cells reduced expression of the target proteins,inhibited proliferation,and sensitized the cells to DDP-induced apoptosis.Conclusion Our findings suggest that hsa-miR-503 modulates cisplatin resistance of human gastric cancer cells at least in part by targeting IGF1R and BCL2.     

Engineering skeletal muscle tissue in bioreactor systems

Objective To give a concise review of the current state of the art in tissue engineering （TE） related to skeletal muscle and kinds of bioreactor environment.Data sources The review was based on data obtained from the published articles and guidelines.Study selection A total of 106 articles were selected from several hundred original articles or reviews.The content of selected articles is in accordance with our purpose and the authors are authorized scientists in the study of engineered muscle tissue in bioreactor.Results Skeletal muscle TE is a promising interdisciplinary field which aims at the reconstruction of skeletal muscle loss.Although numerous studies have indicated that engineering skeletal muscle tissue may be of great importance in medicine in the near future,this technique still represents a limited degree of success.Since tissue-engineered muscle constructs require an adequate connection to the vascular system for efficient transport of oxygen,carbon dioxide,nutrients and waste products.Moreover,functional and clinically applicable muscle constructs depend on adequate neuromuscular junctions with neural calls.Third,in order to engineer muscle tissue successfully,it may be beneficial to mimic the in vivo environment of muscle through association with adequate stimuli from bioreactors.Conclusion Vascular system and bioreactors are necessary for development and maintenance of engineered muscle in order to provide circulation within the construct.     

基于SUV的PET对 4周期化疗后的弥漫大B细胞淋巴瘤患者预后的评估

在过去的十来年中,18氟脱氧葡萄糖（fluorodeoxyglucose,FDG）PET已经成为多种淋巴瘤疗效评价的有力工具,它主要通过视觉分析来评估疗效。修订后的判读标准适合评估化疗结束后的疗效。而不适用于化疗期间的疗效评价。     

益智健脑颗粒联合针灸对阿尔茨海默病大鼠学习记忆的影响

目的观察益智健脑颗粒联合针灸对阿尔茨海默病（Alzheimers disease,AD）大鼠学习记忆的影响。方法将大鼠随机分为假手术组（A组）、模型组（B组）、针灸组（C组）、益智＋针灸组（D组）各10只,B、C、D 3组分别以海马CA1区注射β淀粉样蛋白25-35（Aβ25-35）造模,A组注射等量的双蒸水,各组分别治疗20 d后行Morris水迷宫试验,观察大鼠学习记忆能力变化。结果B组较A组的平均潜伏期明显延长,差异具有统计学意义（P〈0.05）;与B组比较,C组、D组的平均潜伏期明显缩短,过台次数增多,差异具有统计学意义（P〈0.05,P〈0.01）;与C组比较,D组的潜伏期缩短,过台次数增多,差异具有统计学意义（P〈0.05）。结论益智健脑颗粒联合针灸能够提高Aβ25-35介导的AD模型大鼠的学习记忆能力。     

程丑夫教授论治情志病验案举隅

程丑夫是国家级名老中医,湖南中医药大学第一附属医院主任医师、教授、博士生导师,享受政府特殊津贴专家,出身于中医世家,从医40余年,经验丰富,对于内科系统及疑难杂症的治疗颇有心得,笔者有幸跟师学习,聆听教诲,受益匪浅,现将程师论治情志病的经典验案略陈一二。1思虑伤脾案患者肖某,女,27岁。初诊：2014年5月20日。半年前因婚变后出现忧心忡忡,多思多虑,近1月来反复腹部胀满,刻诊：腹胀,食后为甚,呃逆,无反酸,通气后可减轻,无腹痛,不欲食,夜寐不安,二便调。舌红苔厚白腻,脉弦,BP：110／70mmHg。     

Pharmacokinetics and Tissue Distribution of Clevidipine and Its Metabolite in Dogs and Rats

The purpose of the current study was to examine the pharmacokinetic profiles and tissue distribution of clevidipine, an ultra-short-acting calcium antagonist in Beagle dogs and Sprague-Dawley rats, respectively. The pharmacokinetics and biodistribution of its primary metabolite H 152/81 were also evaluated. Dogs received intravenous infusion of clevidipine at a dose rate of 17 μg/（kg·min）, and rats were given intravenous administration of clevidipine at a dose of 5 mg/kg. Dog plasma and rat tissues were collected and assayed by HPLC-MS/MS. It was found that plasma clevidipine quickly reached the steady state concentration. The terminal half-life was short （16.8 min）, pointing out a rapid elimination after the end of the infusion. The total clearance was 5 mL/（min·kg）. In comparison, plasma concentra- tion of H152/81 was increased more slowly and was significantly higher than that of clevidipine. After intravenous administration, clevidipine was distributed rapidly into all tissues examined, with the high- est concentrations found in the brain, heart and liver. Maximal concentrations of clevidipine were found in most tissues at 10 min post-dosing. However, the proportion of clevidipine distributed in all tissues was quite small （0.042‰） compared to the total administration dose. It was suggested that clevidipine was mainly distributed in blood and it transformed to inactive metabolite raoidlv.     

补肾活血方对PCOS大鼠模型卵巢中PAI-1mRNA表达的影响

目的探讨补肾活血方对大鼠PCOS模型卵巢局部纤溶酶原激活物抑制因子-1（PAI—1mRNA）表达的影响。方法选用未成年24d龄SD雌性大鼠60只,随机分为模型组、克罗米酚组、补肾活血方高剂量组、补肾活血方低剂量组、正常对照组5组。用Bogovich法建立大鼠多囊卵巢病理模型。以克罗米酚为对照。用原位杂交法观察补肾活血方对多囊卵巢大鼠局部PAI—1mRNA的影响。结果模型组卵巢局部PAI—1mRNA存在卵泡膜间质细胞显著增高,用补肾活血方高、低剂量与克罗米酚药后,卵巢局部PAI-1mRNA的表达明显降低．差异具有统计学意义（P〈0．05、P〈0．01）。补肾活血方高剂量组与克罗米酚组比较,差异具有显著统计学意义（P〈0．01）;低荆量组与克罗米酚组比较差异无统计学意义（P〉0．05）,补肾活血方高、低剂量组比较,低剂量组卵泡膜间质细胞上PAI-1的基因表达增高更明显,但二者差异无统计学意义（P〉0．05）。结论PAI-1mRNA可能与多囊卵巢综合征的发病机制有关。以补肾活血立法的补肾活血方能降低多囊卵巢大鼠局部PAI—1mRNA的显著增高表达．降低PAI—1mRNA卵巢局部的作用。提示补肾活血方可能通过PAI—1mRNA途径促进卵巢排卵的机制。     

Expression and Significance of Cox-2 and Livin in Oral Squamous Cell Carcinoma with Tissue Microarray

Objective To detect the expression of Cox-2 and livin in oral squamous cell carcinoma and precancerous lesion with tissue microarray, and discuss their significance and relationship in the occurrence and development of oral squamous cell carcinoma. Methods Immunohistochemical （IHC） staining and tissue microarray technique were used to detect the expression of Cox-2 and livin in noma! oral mucous membrane, precancerous lesion and oral squamous cell carcinomas. Results The expression of Cox-2 was negative in normal oral mucous membrane, and positive in precancerous lesion （81.6%） and squamous carcinoma （85.2%）; while the expression of livin was negative or weakly positive in normal oral mucous membrane, and positive in precancerous lesion （89.8%） and squamous carcinoma （100%）. The positive expression of Cox-2 and livin were both closely related to pathological classifications of oral squamous cell carcinomas. But there was no correlation between them. Conclusion Cox-2 and livin have close relationship with the occurrence and development of oral squamous cell carcinoma, but no correlation with the expression.