卡尔曼滤波分光光度法测定复方头孢氨苄胶囊的二组分含量

本文将卡尔曼滤波分光光度法用于复方头孢氨苄胶囊二组分的同时测定，组分头孢氨苄和甲氧苄氨嘧啶的平均回收率分别为：９９．３％ＲＳＤ＝０．５７％和１００．４％ＲＳＤ＝０．７７％。     

卡尔曼滤波分光光度法测定复方头孢氨苄胶囊的二组分含量

本文将卡尔曼滤波分光光度法用于复方头孢氨苄胶囊二组分的同时测定。组分头孢氨苄和甲氧苄氨嘧啶的平均回收率分别为：９９．３％ＲＳＤ＝０．５７％和１００．４％ＲＳＤ＝０．７７％（ｎ＝１５）     

系数倍率分光光度法测定复方头孢氨苄胶囊中头孢氨苄及甲氧苄氨嘧啶含量

系数倍率分光光度法测定复方头孢氨苄胶囊中头孢氨苄及甲氧苄氨嘧啶含量于治国，宋洪杰，尚飞沈阳药学院药学系沈阳１００１５陈宁宁辽凯药业有限公司沈阳ｌ１００１５复方头孢氨苄胶囊为ＣＬＸ（头孢氨苄）和ＴＭＰ（甲氧苄氨嘧啶）的复方制剂．现行药品标准［１］分别采...     

差示分光光度法测定复方头孢氨苄胶囊中甲氧苄氨嘧啶的含量

差示分光光度法测定复方头孢氨苄胶囊中甲氧苄氨嘧啶的含量于治国，宋洪杰，尚飞沈阳药学院药学系沈阳１１００１５朱和英沈阳市良苑制药厂复方头孢氨苄胶囊为头孢氨苄（ＣＬＸ）和甲氧苄氨嘧啶（ＴＭＰ）的复方制剂。现行质量标准［１］采用提取分光光度法测定其中的ＴＭ...     

复方庆大霉素注射液中甲氧苄氨嘧啶的紫外分光光度法测定

复方庆大霉素注射液中甲氧苄氨嘧啶的紫外分光光度法测定蒋奕嵩（广西桂林市医药工业物资供销公司质检科桂林市５４１００１）关键词甲氧苄氨嘧啶；紫外分光光度法；测定复方庆大注射液中甲氧苄氨嘧啶在药典中已有收载［１］，其中ＴＭＰ的含量测定，有两种方法：一是非水...     

一阶导数分光光度法测定复方头孢氨苄胶囊中头孢氨苄的含量

一阶导数分光光度法测定复方头孢氨苄胶囊中头孢氨苄的含量于治国，宋洪杰，尚飞沈阳药学院药学系１１００１５吴小兰，王景春沈阳军区第２０２医院复方头孢氨苄胶囊为ＣＬＸ（头孢氨苄）和ＴＭＰ（甲氧苄氨嘧啶）的复方制剂．现行质量标准［１］采用剩余碘量法测定其中的...     

高效液相色谱法考察复方头孢氨苄胶囊的溶出度

采用反相高效液相色谱外标法,对复方头孢氨苄胶囊溶出度进行考察。方法：采用转篮法,以4％乙酸溶液为溶剂,转速为100r/min,时间为45min。色谱条件：C18反相谱柱为固定相,V（水）：V（甲醇）：V(3．86％乙酸钠溶液）：V(4％乙酸溶液）＝742：240：15：3为流动相,流速为1.2mL/min,紫外检测波长为254nm,在此条件下头孢氨苄淀两组分能很好地分离。本法的线性范围分别是头孢氨苄为：27．6－276μg/mL,甲氧苄啶为：5．56－ 55．6μg/mL,平均回收率为头孢氨苄：99．32％,RSD＝0．78％;甲氧苄啶：100．1％,RSD＝0．57％。     

HPLC法同时测定复方头孢氨苄胶囊中头孢氨苄和甲氧苄啶的含量

目的：以HPLC法同时测定复方头孢氨苄中的头孢氨苄与甲氧苄啶的含量。方法：HPLC外标法,采用岛津C18反相色谱术,以V（水）：V（甲醇）：V（3.86%乙酸钠溶液）：V（4％乙酸溶液）＝742：240：15：3为流动相,流速1.2mL/min,在波长254nm处进行检测。结果：本方法可同时测定两组分的含量。被测组分能很好地分离;头孢氨苄在37.19-371.9ug/ml,甲氧苄啶在8.0-80.0ug/ml范围内,峰面积与其浓度呈良好线性关系;平均回收率依次为99.02%,RSD＝0.19%;99.81%,RSD＝0.15%。结论,以HPLC外标法同时测定复方头孢氨苄中两组分的含量,方法简便、可靠,重现性良好。     

正相硅胶反相洗脱色谱法测定复方黄连素注射液的含量

采用未改性硅胶作固定相的高效液相色谱法同时测定复方黄连素注射液中的硫酸氢小檗碱和甲氧苄氨嘧啶（ＴＭＰ）的含量。实验表明，以甲醇－２％三乙胺水溶液（磷调节ｐＨ至７．０）（８０：２０）作为流动相，得到满意的分离效果。该两组分的回收率分别为１００．８％和９９．９％，ＲＳＤ均不大于１．０％（ｎ＝５）。     

一阶导数光谱法测定增效黄连素胶囊中甲氧苄氨嘧啶的含量

一阶导数光谱法测定增效黄连素胶囊中甲氧苄氨嘧啶的含量尚秀敏，田兰，郭毅河北省药品检验所（０５００１１）关键词一阶导数光谱法；增效黄连素胶囊；甲氧苄氨嘧啶增效黄连素胶囊是由盐酸黄连素与甲氧苄氨嘧啶组成的复方制剂。地方标准（河北省药品标准）中甲氧苄氨嘧啶...     

正交函数分光光度法测定复方头孢氨苄胶囊中头孢氨苄含量

本文采用正交函数分光光度法，以二次正交多项式（ｊ＝２），波长点数Ｎ＝６，波长间隔△λ＝４ｎｍ，中间波长λｍ＝２６６．４ｎｍ，测定复方头孢氨苄胶囊中头孢氨苄的含量．方法平均回收率为９９．２％（ＲＳＤ＝０．６％，ｎ＝９），与现行药品标准方法比较，结果不存在显著性差异（Ｐ＝０．９５）．     

头孢氨苄和头孢哌酮对肾功能及环孢素血浓度的影响

观察了头孢氨苄对肾移植术后患者的肾功能和环孢素血浓度的影响。42例患者在服用头抱氨苄期间,血尿素氮(BUN)、血清肌酐(SCr),环孢素血浓度比合并用药前分别增加2.55％、6.86％及30.56％,比停用头孢氨苄后分别增加17.59％,39.94％及35.80％。而在32例肾移植术后患者进行的另一项研究则表明,合并用头孢哌酮期间。BUN,SCr、环孢素血浓度与用药前、停药后相比几乎没有改变。提示在肾移植木后抗感染治疗可优选头孢哌酮。     

Dipeptide transport: an active process with energy- and proton-dependence in the human intestinal cell line, Caco-2

Transport of protein in the form of small peptides(di/tripeptides)across the small intestinal wall is amajor route of dietary protein absorption.The dipeptidetransporter,PepT1,is known to be located in the in-testine and the kidney,and plays an important …     

II. In vitro studies of antibacterial activity

One hundred and forty isolates of beta-hemolytic streptococcus cultured from patients with clinical pharyngitis were studied by disc diffusion for antibiotic sensitivity to lincomycin, erythromycin, cephalexin and penicillin and by agar dilution to cephalexin and penicillin. All isolates were sensitive to ≤ 0.1 μg./ml. penicillin and ≤ 1.56 μg./ml. cephalexin. The disc-diffusion test was reliable in predicting the sensitivities in vitro. One strain of group A betahemolytic streptococcus was resistant to erythromycin by disc diffusion. When compared to Lancefield grouping 18% of strains were incorrectly identified as group A by the bacitracin-disc test. Cephalexin was uniformly effective in vitro in inhibiting beta-hemolytic streptococci and the 30 μg. cephalexin disc was reliable in predicting these sensitivities.     

头孢氨苄片溶出度的光纤过程分析

目的：对头孢氨苄片体外溶出过程进行分析,建立以光纤药物溶出仪为测试仪器的药物质量评价的方法学。方法采用 FODT-601型光纤药物溶出度实时测定仪检测头孢氨苄片的溶出度。结果仪器可平行、实时监测6个通道不同头孢氨苄片的溶出过程,日内、日间精密度分别为1．87％和0．85％;回收率为100．03％,RSD为3．91％;FODT 法测得头孢氨苄片平均溶出度为（110．0±2．07）％,与紫外-可见分光光度法测得结果无明显差异。结论光纤药物溶出仪可实时监测片剂的体外溶出情况,实验数据可靠,在考察制剂溶出曲线、优化处方工艺上发挥重要作用。     

ENTERIC FEVER – CULTURE AND SENSITIVITY PATTERN AND TREATMENT OUTCOME

One hundred cases of enteric fever in the age group of 6 months to 12 years were analysed with respect to culture sensitivity pattern and treatment outcome. Patients were divided into 5 treatment groups – chloramphenicol, amoxycillin, trimethoprim-sulfamethoxazole + furazolidine, gentamicin + cephalexin and ciprofloxacin. Out of 91 culture positive cases, 100% were sensitive to ciprofloxacin followed by gentamicin (84.9%), cephalexin (83.6%), furazolidine (36.6%), trimethoprim-sulfamethoxazole (34.1%), chloramphenicol (34.0%) and amoxycillin (23.8%). In 60 cases resistant to chloramphenicol, resistance to other drugs varied from 20 to 88.3%. The treatment response was 100% to ciprofloxacin, 72.7% to chloramphenicol, 50% to gentamicin + cephalexin, 38.5% to trimethoprim-sulfamethoxazole + furazolidine and 12.5% to amoxycillin. Out of 48 cases who did not respond to initial regimen, 33 were treated successfully with ciprofloxacin and remaining with other drug regimens. Time taken for defervescence was shortest with gentamicin + cephalexin (4.6±2.0 days) followed by ciprofloxacin (6.1±2.5 days) and chloramphenicol (6.4±3.5 days). There were 3 deaths in this study.KEY WORDS: Typhoid, Drug resistance microbial, Clinical trials     

Cephalexin--a new oral antibiotic

The pharmacology, antimicrobial activity and clinical uses of cephalexin, a semi-synthetic derivative of cephalosporin C, are described.     

弗劳地枸橼酸杆菌引起的猕猴腹泻

对从腹泻猕猴脓血便中分离的一株细菌，经ATB细菌分析仪，选用ID32E肠杆菌测试条鉴定为弗劳地枸橼酸杆菌(Citrobacter freundii)，id%=99．9。用K-B纸片法做药敏试验，结果表明，该菌对诺氟沙星、环丙沙星、头孢噻肟、头孢曲松、头孢他定、氯霉素敏感。对庆大霉素、头孢拉定、痢特灵等十几种抗生素耐药。总之，在做腹泻病猴粪便培养时，应重视弗劳地枸橼酸菌的检测。     

Caco-2细胞缺氧复氧损伤后二肽载体表达及生物学功能的改变

目的：探讨Caco-2细胞缺氧复氧（anoxia/reoxygenation,A/R)损伤后二肽载体表达及生物学功能的变化。方法：采用Caco-2细胞A／R方法，模拟临床缺血再灌注（ischemia/reperfusion injury,I/R)。Caco-2细胞正常培养和A／R后（缺氧90min，复氧30min)后，测定二肽载体对头孢氨苄的摄取功能；采用流式细胞术观察损伤后Caco-2细胞的凋亡情况;员时比较两种培养情况下二肽载体的mRNA水平。结果：A／R后二肽载体对头孢氨苄的摄取能力下降；损伤后Caco-2细胞凋亡水平较正常细胞明显提高；Northern blotting显示损伤后二肽载体mRNA水平下调。结论：A／R后Caco-2细胞二肽载体mRNA下降从基因水平下调了二肽载体对头孢氨苄的摄取功能。提示Caco-2细胞A／R或临床I／R后肠上皮细胞刷状缘二肽载体对二肽的摄取功能下降。     

I. Clinical evaluation

A study was undertaken to evaluate the therapy of streptococcal pharyngitis. The compliance of 118 patients with beta-hemolytic streptococcal pharyngitis to follow-up was 72%. Of 74 patients checked by means of urine tests 66 took their oral medication. No differences were detected in the clinical and bacteriological results (>98% streptococcal eradication) after the 7th or 10th day of therapy after taking either cephalexin or penicillin.

It was concluded that: (a) for effective surveillance and follow-up special attention should be given to the uncooperative segment of the patient population; (b) a seven-day course of penicillin may be satisfactory in the eradication of BHS from the throat; and (c) cephalexin appears to be an effective alternative to penicillin for the treatment of streptococcal pharyngitis.