共查询到19条相似文献,搜索用时 62 毫秒
1.
金鸡菊提取物体外抗氧化活性 总被引:20,自引:8,他引:12
目的:探讨金鸡菊提取物体外抗氧化活性。方法:采用分光光度法,测定金鸡菊提取物对羟基(OH)自由基、超氧阴离子(O 2-.)自由基及1,1-二苯基-2-苦肼基自由基(DPPH)的清除能力,通过多元线性回归分析,考察金鸡菊提取物含量及其体外抗氧化活性的相关性,并同维生素(VC)进行了比较。结果:金鸡菊提取物对.OH自由基,O 2-.自由基,DPPH自由基均有较好的清除作用,在供试质量浓度范围内(0.2~1.0 g.L-1)对各自由基的清除效率与金鸡菊提取物浓度有一定量效关系,当质量浓度达1.0 g.L-1,其中50%乙醇洗脱物对OH自由基、O 2-.自由基和DPPH自由基的清除率可达86.19%,97.90%和71.85%,且清除能力与Vc相当。结论:金鸡菊提取物具有显著的体外抗氧化作用。 相似文献
2.
3.
10种药用植物提取物抗氧化活性的研究 总被引:1,自引:0,他引:1
利用DPPH比色法,以VE为参数,测定比较10种药用植物甲醇提取物对DPPH自由基清除能力。结果表明在10种药用植物中岩白菜根茎提取物抗氧化活性最高,其自由基清除效率(SE)是VE的2倍,有望成为天然抗氧化剂的素材。 相似文献
4.
酢浆草提取物体外抗氧化活性研究 总被引:4,自引:0,他引:4
目的:探讨酢浆草提取物体外抗氧化活性。方法:采用DPPH法、FRAP法分别测定酢浆草水提取物清除DPPH自由基和总的抗氧化能力。结果:在实验的测定浓度范围之内,随着提取物浓度的增加,其对DPPH自由基的清除能力逐渐增强。2mg/mL酢浆草水提物对DPPH自由基的清除率达到67.144%。FRAP法测得20mg/mL酢浆草水提取液的总抗氧化能力为1.09mM。结论:酢浆草对DPPH自由基的清除效果良好,FRAP法测得酢浆草有一定的抗氧化能力,有进一步开发利用的价值。 相似文献
5.
目的:考察谷精草水提物和醇提物的抗氧化活性。方法:采用UV检测谷精草水提物和醇提物对超氧阴离子自由基、羟基自由基和DPPH自由基的清除能力,通过普鲁士法测定提取物还原Fe3+的能力,并以丁基羟基茴香醚(BHA)为参比测定提取物对金属离子(Fe2+)的螯合能力。结果:谷精草水提物和醇提物对3种自由基均具有较强的清除作用,水提物的半数抑制浓度(IC50)分别为1.389,3.507,0.341 g·L-1,醇提物的IC50分别为0.792,5.525,0.192 g·L-1。提取物对Fe3+有还原能力,对金属离子(Fe2+)有较强的螯合能力,且随着提取液质量浓度的增加而增强,呈剂量效应关系。结论:谷精草水提物和醇提物均具有较强的抗氧化活性,在相对低质量浓度下醇提物的抗氧化作用比水提物强。 相似文献
6.
周静 《国外医药(植物药分册)》2007,22(5):213-214
〔英〕/Kim S Y…∥Food Chem.-2006,97(3).-472~479评价了葡萄籽原状和粉末状材料分别在50、100、150、200℃加热后,对其乙醇提取物(GSE)清除自由基活性(RSA)和还原能力的影响。葡萄籽清洗后晒干,取部分加工成均化的微粒状粉末。将葡萄籽和葡萄籽粉分别在50、100、150、200℃ 相似文献
7.
8.
9.
10.
11.
复方丹参的体外抗氧化活性研究 总被引:24,自引:0,他引:24
目的探讨复方丹参的体外抗氧化活性。方法采用DPPH自由基、超氧阴离子、羟基自由基、鳌合亚铁离子和还原力、过氧化氢的反应体系,检测了复方丹参的体外抗氧化活性,并与维生素C进行比较。结果复方丹参和维生素C消除DPPH自由基的EC50分别是41.0μg/mL和8.2μg/mL;复方丹参消除超氧阴离子自由基的EC50是1.39 m g/mL;复方丹参和维生素C抑制羟基自由基产生的EC50分别是1.73 m g/mL和0.58 m g/mL;在实验体系中,未检测到复方丹参螯合亚铁离子的能力;复方丹参和维生素C的还原能力的EC50分别是0.42 m g/mL和0.08 m g/mL。在实验最高质量浓度100μg/mL时,复方丹参和维生素C对过氧化氢的清除率分别为36%和94%。结论复方丹参具有较强还原力,能清除DPPH和超氧阴离子自由基的作用,并抑制羟基自由基的产生。复方丹参的多方面抗氧化活性可能是在治疗心血管疾病效果显著的原因之一。 相似文献
12.
苦参提取物体外抗菌实验研究 总被引:6,自引:0,他引:6
目的:探讨苦参总生物碱、总黄酮提取物及其混合物的体外抗菌效果。方法:采用液体二倍稀释法分析苦参总生物碱、苦参总黄酮提取物及其混合物对金黄色葡萄球菌、大肠埃希菌、福氏志贺菌、乙型副伤寒杆菌、铜绿假单胞菌、白色葡萄球菌、痢疾杆菌和柠檬色葡萄球菌的抗菌作用,并测定最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果:苦参总生物碱对以上菌株的MIC分别为:9.00、3.38、8.25、9.00、4.50、13.50、6.75、7.13mg.mL-1,MBC分别为:13.50、14.25、8.25、9.00、14.25、14.25、13.13、14.25mg.mL-1;苦参总黄酮对以上菌株的MIC分别为3.00、2.63、3.56、3.38、1.50、2.44、3.56、2.35mg.mL-1,MBC分别为14.25、12.75、7.13、13.50、13.50、12.75、12.75、13.13mg.mL-1;苦参总生物碱与总黄酮混合物对以上菌株的MIC分别为:2.25、2.07、3.28、3.94、1.22、2.44、2.07、1.97mg.mL-1,MBC分别为9.00、13.13、6.75、6.38、13.88、7.50、6.38、7.13mg.mL-1。结论:苦参总生物碱和总黄酮提取物对上述菌株存在体外抑菌和杀菌活性,混合物呈现一定协同作用。 相似文献
13.
Background:Vine tea from fermented Ampelopsis grossedentata leaves has been used as a herbal tea and folk medicine in the southern region of China for hundreds of years.The aim of this investigation was to analyze the total flavonoids found in vine tea,including three bioactive flavonoids,and the total phenolic contents in the aqueous methanol extracts of 10 vine tea samples.In addition,this study also aimed to examine the antioxidant activity of dihydromyricetin and vine tea’s flavonoid-rich extract.Methods:The total flavonoids and total phenolic content assay of extracts from vine tea were performed by ultraviolet-visible spectroscopy and epoch microplate spectrophotometer,respectively.Three bioactive flavonoids were quantified simultaneously using high performance liquid chromatography.The antioxidant activity of dihydromyricetin and vine tea’s flavonoid-rich extract was evaluated in vitro using six different methods.Results:Vine tea contained a large number of flavonoids,with dihydromyricetin as its main constituent.The flavonoid-rich extract exhibited a significant scavenging effect on superoxide anion radicals,and on 3-ethylbenzthiazoline-6-sulphonic acid and 1,1-diphenyl-2-picrylhydrazyl radicals.It also possessed definite activity in lipid peroxidation inhibition,ferric reduction,and the moderation of Fe2+ion chelation ability.There was a significant negative correlation between dihydromyricetin content and antioxidant activity in the vine tea samples,including superoxide anion radical scavenging activity(P=−0.754,P<0.05),lipid peroxidation inhibition activity(P=−0.759,P<0.05),ferric-reducing antioxidant power(P=−0.843,P<0.01),respectively.Dihydromyricetin played a dominant role in the antioxidant activities of the flavonoid-rich extract.Conclusion:Vine tea’s flavonoid-rich extract could be used as a new antioxidant source to safeguard against oxidative stress. 相似文献
14.
AIM: Considering the importance of diet in the prevention of cellular damage caused by reactive oxygen species which has been implicated for several diseases, this present study was undertaken to evaluvate the in vitro and in vivo antioxidant potential of the ethanolic extract of the fruiting bodies of Ganoderma lucidum on 7, 12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinogenesis in Sprague Dawley rats. METHODS: Ganoderrna lucidum extract was tested for in vitro antioxidant and radical scav- enging assays, such as (ABTS^-1) radical cation decolorization assay, DPPH radical scavenging, hydroxyl radical, and superoxide radical scavenging assays. The in vivo antioxidant potentials were analyzed by SOD, CAT, and GPx in plasma, mammary, and liver tissues. RESULTS: In all the in vitro antioxidant and radical scavenging assays the extract exhibited good scavenging activity. In vivo enzy- matic antioxidant levels, such as SOD, CAT, and GPx were decreased in DMBA-induced animals. Moreover, pretreatment with G. lucidum (500 mg.kg-1 bw) to DMBA-induced animals significantly (P 〈 0.05) increased the levels of SOD, CAT, and GPx in plasma, mammary, and liver tissues compared to DMBA induced animals. CONCLUSIONS: From these findings, it is suggested that G. lu- cidum extract could be considered as a potential source of natural antioxidants and can be used as an effective chemopreventive agent against mammary cancer. 相似文献
15.
Fejes S Blázovics A Lugasi A Lemberkovics E Petri G Kéry A 《Journal of ethnopharmacology》2000,69(3):259-265
Standardised aqueous extracts of chervil (Anthriscus cerefolium L. Hoffm.) (Apiacae) were investigated for antioxidant effect. Numerous in vitro test methods were used to determine whether the extracts, from different vegetative parts (root, herb) had H-donor, metal binding, reductive, free radical scavenging and membrane protective activity. Apiin was used as a reference material. The herb extract showed better activity in all experiments than the root extract. The present results underline that the wateric chervil extracts have antioxidant and anti-lipoperoxidant activity. 相似文献
16.
Debenedetti S Muschietti L van Baren C Clavin M Broussalis A Martino V Houghton PJ Warhurst D Steele J 《Journal of ethnopharmacology》2002,80(2-3):163-166
Fifteen extracts from nine selected Argentine medicinal plants were tested for their antiplasmodial activity in vitro by assessing their ability to inhibit the uptake of [3H]-hypoxanthine into the Plasmodium falciparum K1 pyrimethamine/chloroquine resistant strain. The methanol extract of Satureja parvifolia showed good antiplasmodial activity (IC50 3 μg/ml). Inhibition of the growth of P. falciparum was also observed with aqueous extracts of Buddleja globosa and S. parvifolia. 相似文献
17.
[目的]比较银杏叶提取物中三个主要的银杏黄酮苷元体外及经肠吸收后抗氧化活性。[方法]采用1,1-二苯基-2-三硝基苯肼(DPPH)法(1,1-二苯基-2-三硝基苯肼法)测定模型药物体外及肠吸收液对自由基的清除作用,测定药物的抗氧化活性。[结果]槲皮素、异鼠李素、山奈酚体外清除DPPH自由基的EC50分别为78.6μm,203.2μm,222.0μm;药物肠吸收液DPPH清除率趋势为:槲皮素异鼠李素山奈酚,其清除率较体外相比有所降低;3个单体不同配比的肠吸收液DPPH清除率显示以提取物中所含比例混合清除率数据高于其他两组。[结论]3个单体体外及肠吸收液均能够清除DPPH自由基,且清除能力为:槲皮素异鼠李素山奈酚;肠吸收后药物清除率下降,可能与单体在肠道中产生分解有关,其原因还需进一步探讨,3个单体以提取物所含比例混合后抗氧化活性要好。 相似文献
18.
19.
AIM:To evaluate in vitro anti-oxidant potential of methanolic and aqueous extract of Indian habitants Anisochilus carnosus (L.F.) wall.,which is an annual shrub belonging to the family Lamiaceae commonly known asmint family.METHODS:The present study describes the effects by DPPH,alkaline DMSO,scavenging of ferric ion,NO,H 2 O 2,ABTS models.Both extracts showed good free radical scavenging property which was calculated as IC 50.RESULTS:IC 50 of aqueous and methanolic extracts were found to be 7.09 and 5.77 μ... 相似文献