5种牙本质粘结剂的细胞毒性研究

目的比较Clearfil S3 Bond和Single Bond,Clearfil SE Bond,XenoⅢBond,G Bond对L929细胞活力的影响。方法小鼠成纤维细胞L929在Clearfil S3 Bond,Single Bond,Clearfil SE Bond,XenoⅢBond,G-Bond五种牙本质粘结剂的不同体积分数浸渍液(100%、50%、25%、12.5%)作用不同时间(24 h、72 h、120 h)后,倒置相差显微镜观察细胞形态变化,MTT法测定细胞相对增值率(RGR);用5级毒性分类法评级,并进行统计分析。结果 5种牙本质粘结剂在不同体积分数浸渍液的作用下,L929细胞形态均发生不同程度的变化。在72 h、120 h后,12.5%Clearfil S3 bond组和Clearfil SE Bond组的RGR值显著高于其它各组的RGR值(P<0.05)。结论 5种牙本质粘结剂的体外细胞毒性均较弱,其中第7代牙本质粘结剂Clearfil S3Bond和第5代牙本质粘结剂Clearfil SE Bond的细胞毒性显著低于其他几种粘结剂,可以安全地应用于临床。     

4种牙本质粘结剂对牙髓成纤维细胞毒性作用的比较

目的:对比研究4种第七代牙本质粘结剂对人牙髓成纤维细胞的毒性作用,初步探讨其生物安全性。方法:组织块培养法原代培养人牙髓成纤维细胞,免疫组织化学染色SP法鉴定细胞来源,采用四甲基偶氮唑盐比色法和浸提液法,双盲观察4种新一代牙本质粘结剂(G-Bond、i-Bond、xeno V、Clearfil S3Bond)的不同体积分数浸提液(12.5%、25%、50%、100%)作用不同时间(24、48、72 h)对人牙髓成纤维细胞的毒性作用。结果:4种牙本质粘结剂不同体积分数浸提液的作用下,人牙髓成纤维细胞形态均发生不同程度的变化。24、48 h时,xeno V细胞毒性影响较小,i-Bond细胞毒性影响较大,G-Bond与Clearfil S3 Bond细胞毒性无明显差异;72 h时,4种牙本质粘结剂对细胞毒性影响无明显差异。结论:4种牙本质粘结剂毒性趋于0～2级,随着作用时间延长,细胞毒性无明显差异。     

五种牙本质粘接剂体外细胞毒性研究

目的 对5种牙本质粘接剂的体外细胞毒性进行对比研究,为临床应用提供参考依据.方法 采用体外细胞培养技术和四甲基偶氮唑盐(MTT)比色法,观察第7代牙本质粘接剂Clearfil tri-S Bond和4种第6代牙本质粘接剂Clearfil SE Bond、Adper Prompt、ONE UP BOND F、XenoⅢ粘接剂浸提液对L929小鼠成纤维细胞生长的影响,测定各组吸光度值(OD值),计算L929小鼠成纤维细胞的相对增殖率,用5级毒性分类法评级,并进行统计分析.结果 各组细胞生长均较好,Clearfil tri-S Bond组和Clearfil SE Bond组的OD值显著高于其它各组的OD值.结论 5种牙本质粘接剂的体外细胞毒性均较弱,其中第7代牙本质粘接剂Clearfil tri-S Bond和第6代牙本质粘接剂Clearfil SE Bond的细胞毒性最低.     

4种常见牙本质黏结剂的边缘封闭性研究

目的:研究对比4种临床常用牙本质黏结剂的边缘封闭性.方法:12个离体前磨牙,在颊、舌侧牙颈部釉-牙骨质界处各制备直径2 mm,深1.5 mm盒型洞.随机分为4组,分别使用Single Bond、AdperPrompt、Clearfil SE Bond、Spread iBond黏结,3M Z250树脂充填.500 s/L碱性硝酸银中浸泡24 h,强光显影8 h,沿牙体长轴通过充填体正中颊舌向纵行剖开,体视显微镜下观察各剖面充填体边缘的微渗漏情况,并以扫描电镜观察树脂-牙本质界面的结合状态.结果:各种黏结剂界面均存在微渗漏现象,实验组Single Bond、AdperPrompt、Clearfil SE Bond和Spread iBond渗漏深度分别为(777.23±120.13)μm、(930.12±130.10)μm、(240.33±120.53)μm、(640.12±170.23)μm.Clearfil SE Bond组银渗漏的深度明显低于其他组,(P<0.05),其他3组组间无显著性差异(P>0.05).结论:临床上常用的4种黏结剂均不能避免牙本质黏结界面的渗漏,Clearfil SE Bond对牙本质的封闭性优于Adper Prompt、Spread iBond、Single Bond.     

5种牙本质粘接剂的细胞毒性与单体双键转化率的关系

目的 比较5种牙本质粘接剂的细胞毒性,探讨影响其差异的相关因素及其与单体双键转化率的关系。方法 选择5种临床常用的商品牙本质粘接剂OptiBond FL（FL）、Single Bond 2（SB）、Prime&Bond NT（PB）、Clearfil SE Bond（SE）和Adper Easy One（EO）,采用MTT法检测材料的细胞毒性,运用荧光染色法观察细胞形态。采用傅里叶红外光谱测量5种牙本质粘接剂的单体双键转化率。结果 牙本质粘接剂浸提1 d时,MTT检测得到的OD值从大到小依次为SE > C ≈ FL ≈ EO ≈ PB > SB,其中SB组的吸光度值明显低于对照组,且差异具有统计学意义（P<0.05）;牙本质粘接剂浸提3 d和5 d时,SB组和EO组的吸光值远低于对照组,呈现明显的细胞毒性。牙本质粘接剂单体双键转化率值从大到小依次为PB > EO > FL > SB > SE,相邻两组间差异有统计学意义（P<0.05）。但各组分本质粘接剂的细胞毒性和单体双键转化率的相关性无统计学意义（P>0.05）。结论 不同牙本质粘接剂的细胞毒性不同,与其单体双键转化率不存在线性关系,两者均受到多种因素的影响。     

人工唾液条件下3种自酸蚀粘接剂与牙本质粘接耐久性研究

目的　探讨Single bond™ Universal等3种不同类型自酸蚀粘接剂与牙本质的粘接强度和耐久性。方法　选取 2016年5—7月解放军总医院口腔颌面外科拔除的24颗新鲜人离体第三磨牙,随机分为3组,每组8颗,分别选用Clearfil SE Bond（SE组）、G-Bond plus（GBp组）、Single bond™ Universal（SBU组）等3种自酸蚀粘接剂与新鲜牙本质粘接并制备试件。每组挑选出完整试件160个,再分为两个亚组（各80个试件）,分别在即刻与人工唾液浸泡6个月后行微拉伸粘接强度实验,体视显微镜下观察试件断裂类型。 结果　SE组、GBp组、SBU组即刻牙本质粘接强度分别为（55.70 ± 11.07）、（52.85 ± 7.54）和（62.82 ± 8.70）MPa,其中SBU组高于SE组和GBp组,差异有统计学意义（P < 0.05）。人工唾液保存6个月后,SE组、GBp组、SBU组牙本质粘接强度分别为（13.51 ± 4.99）、（43.53 ± 6.82）和（49.13 ± 6.58）MPa,3组之间差异均有统计学意义（P < 0.05）。6个月时粘接界面观察,SE组断裂模式均为粘接界面断裂;GBp组16个试件为牙本质内聚断裂,4个试件为复合树脂内聚断裂;SBU组28个试件为牙本质内聚断裂,8个试件为复合树脂内聚断裂。 结论　Single bond™ Universal即刻牙本质粘接强度和人工唾液老化后6个月粘接强度均显著高于Clearfil SE Bond、G-Bond plus;Single bond™ Universal粘接强度大,稳定耐久,可提供良好的粘接效果。     

三种树脂粘接剂体外细胞毒性研究

目的：对三种不同树脂粘接剂的体外细胞毒性进行比较，以期为临床应用提供依据。方法：采用体外细胞培养技术和四唑盐显色(MTT)方法，将三种粘接剂浸提液与L929细胞接触2、4、7d后，用酶联免疫仪测定光吸收值，计算细胞相对增殖率，用五级毒性分类法评级，并统计分析。结果：4d及7d时Durafill Bond、Prime＆Bond NT毒性级1-2级，Clearfil SE Bond毒性级1级，三者之间有显著性差异，且有浓度、时间依赖性。结论：三种粘接剂有轻微细胞毒性，其中Clearfil SE Bond毒性最低。     

不同类型牙本质粘接系统中单体Bis-GMA的析出动力学研究

目的:利用反相高效液相色谱法定量分析不同牙本质粘接体系中Bis-GMA随时间变化的析出量。方法:15个牙本质片(8 mm×8 mm×1 mm)根据选用的牙本质粘接剂随机分为Single Bond 2(SB)、Clearfil SE Bond(SEB)和Clearfil 3SBond(3SB)3组,分别按使用说明进行粘接操作后储存于37℃、75%的酒精中浸泡老化,于10 min、30 min、1 h、3 h、6 h、24h、48 h、72 h、7 d、14 d各取每个试件浸泡的老化液进行反相高效液相色谱议(HPLC)检测,对结果数据做单因素方差分析。结果:3种不同类型的粘接系统中均存在未聚合单体Bis-GMA的析出,且主要集中在操作完成后的24 h内,以最初10 min内的析出量最高,SEB组>3SB组>SB组(P<0.01)。结论:不同类型牙本质粘接系统内的单体Bis-GMA在75%的酒精中的析出动力学基本类似。     

五种牙本质粘接剂对乳牙牙本质边缘封闭性的比较

目的 检测5种牙本质粘接剂对乳牙牙本质的粘接性能,为牙本质粘接剂的临床使用提供依据.方法 将50颗因滞留而拔除的乳前牙按随机数表法分为5组,每组10颗.用棒状金刚砂车针EX-41在唇面中1/3处制备约直径1.5mm、深1mm的Ⅰ类洞,各组分别使用FL-BondⅡ、Clearfil Protect Bond、Clearfil SE Bond、AdperTMEasy One和AdperTM Single Bond 2,每组分别按说明进行粘接,树脂充填.冷热循环5000次,体视镜下观察充填物边缘染料渗入的深度、分级进行统计.结果 各组均发生微渗漏,各组间微渗漏程度差异无统计学意义(P＞0.05).结论 AdperTM Easy One组的边缘封闭性与其他4组相同,由于操作简便更适用于儿童乳牙的粘接.     

牙本质粘结剂对人牙髓细胞毒性的体外研究

目的：评价全酸蚀牙本质粘结剂和自酸蚀牙本质粘结剂对人牙髓细胞的毒性作用.方法：用人牙髓细胞为实验细胞,采用MTT比色分析法,对4 种牙本质粘结剂（Prime ＆ Bond NT,Single Bond,Xeno Ⅲ,iBond）进行体外细胞毒性研究.结果：不同浓度的粘结剂稀释液均可使人牙髓细胞的形态有所改变.4 种牙本质粘结剂的细胞毒性有显著性差异,且作用时间和浓度的改变对其细胞毒性有影响.全酸蚀粘结剂比自酸蚀粘结剂的细胞毒性强.结论：4 种牙本质粘结剂在体外对人牙髓细胞均有一定程度的细胞毒性,其中Single Bond的毒性较强,临床使用粘结剂时应合理选择粘结剂和掌握固化时间.     

The effect of different air-polishing powders on dentin bonding

PURPOSE: Air-polishing is routinely used for professional tooth cleaning. Therefore, the aim of this in vitro study was to evaluate the influence of different air-polishing powders on dentin bonding. MATERIALS AND METHODS: Dentin bond strengths of a resin composite (Clearfil AP-X) bonded with different classes of adhesives (4-step etch-and-rinse: Syntac; 3-step etch-and-rinse: OptiBond FL; 2-step etch-and-rinse: Single Bond Plus; 2-step self-etching: AdheSE, Clearfil SE Bond, Clearfil Protect Bond, One Coat Self-Etch Bond; all-in-one/mix: Xeno III; all-in-one/no mix: Clearfil S3 Bond, G-Bond) were measured on flattened dentin surfaces. In test groups, specimens were air polished with Prophypearls (calcium carbonate) or ClinPro Prophypowder (glycine) prior to bonding. Microtensile bond strengths were evaluated after 24 h of storage at 37 degrees C in water. Surfaces and resin-dentin interfaces were observed by SEM, TEM, and CLSM. RESULTS: Mean bond strengths varied from 18 to 73 MPa in control groups. Calcium carbonate air polishing generally caused significantly reduced dentin bond strengths (p < 0.05). TEM exhibited severe nanoleakage after calcium carbonate air polishing. Glycine did not affect dentin bonding performance of the adhesives under investigation. CONCLUSION: Calcium carbonate air polishing should be avoided when dentin-bonded restorations are applied. Glycine powder is an alternative.     

Zinc-doped dentin adhesive for collagen protection at the hybrid layer

The aim of the study was to ascertain whether the addition of zinc to adhesives may decrease metalloproteinase-mediated collagen degradation without affecting bonding efficacy. Human dentin beams were treated with phosphoric acid, with Clearfil SE Bond Primer or with Clearfil SE Bond Primer plus ZnCl(2) (2 wt%). Acid-etched dentin was infiltrated with Single Bond, Single Bond plus ZnCl(2) (2 wt%), or Single Bond plus ZnO nanoparticles (10 wt%), and Clearfil SE Bond-primed dentin was infiltrated with Clearfil SE Bonding resin, Clearfil SE-Bonding resin with ZnCl(2) (2 wt%), or Clearfil SE-Bonding resin with ZnO nanoparticles (10 wt%). The C-terminal telopeptide concentrations were determined 24 h, and 1 and 4 wk after treatment. Microtensile bond strength to dentin was determined for the tested adhesives. Matrix metalloproteinases-mediated collagen degradation occurred in acid-etched and SE-primed dentin. Resin infiltration decreased collagen degradation. Lower collagen degradation was found for SE Bond than for Single Bond. Zinc-doped Single Bond resin always reduced collagen degradation, the ZnO particles being more effective than ZnCl(2) . Zinc-doped SE Bond reduced the liberation of C-terminal telopeptide only at 24 h. Bond strength to dentin was not decreased when Zn-doped resins were employed, except when ZnCl(2) was added to SE Primer. Zinc-doped resin reduced collagen degradation in Single Bond hybrid layers, but did not affect bond strength. The addition of zinc to SE Bond had no beneficial effects.     

HEMA diffusion from dentin bonding agents in young and old primary molars in vitro.

OBJECTIVE: The objective of this study was to evaluate and compare amounts of HEMA diffusion from three dentin bonding systems in young and old primary molars at a remaining dentin thickness (RDT) of 1 mm. METHODS: Occlusal cavities were prepared with RDT of 1 mm in young and old primary molars. Chambers that contain 1 ml of distilled water were attached to each tooth. The groups were treated with Total Etch+Syntac Single Component, Syntac Single Component and Clearfil SE Bond. Water eluates were analyzed by HPLC at 4 min, 24 h and 72 h. RESULTS: The cumulative HEMA release amounts were significantly different among all groups (p<0.05). Acid-etching significantly increased pulpward diffusion of HEMA and young primary teeth presented higher dentin permeability. SIGNIFICANCE: Acid-etching significantly increases pulpward release of HEMA from dentin bonding systems in primary molar teeth. Young primary molars have higher dentin permeability; so the pulp should be protected with cavity liners in deep cavities. However, the dentin bonding systems can be considered safe for clinical use in primary teeth, even in deep cavities, since the released HEMA amounts are below the level of cytotoxicity.     

Effective bond strength of current adhesive systems on deciduous and permanent dentin

PURPOSE: To evaluate the bond strength of a total-etching, self-priming system (Single Bond) and a self-etching system (Clearfil SE Bond) to deciduous and permanent human dentin. METHODS AND MATERIALS: Buccal dentin discs were prepared with a diamond disc from permanent first premolars, permanent third molars and deciduous second molars. The flat dentin surfaces were obtained by polishing with wet 600 grit silicon carbide papers. The specimens of each group were further divided into two groups for bonding to either Single Bond or Clearfil SE Bond. After 24 hours, the microshear bond strength testing was executed on a universal testing machine. Statistical analysis was performed at alpha = 0.05. RESULTS: No significant differences in bond strength were found between materials. However, deciduous dentin demonstrated significantly lower bond strengths than permanent premolar dentin when Single Bond was applied (p < 0.05). CONCLUSION: The difference in bonding substrate (permanent or deciduous dentin) had a significant effect on bond strength when the total-etching, self-priming system was applied.     

牙本质粘接剂粘接强度与界面形态的研究

目的:采用一种新的界面处理方法,对不同类型牙本质粘接剂的粘接强度与界面形态进行研究.方法:选取人无龋磨牙,暴露咬合面牙本质,分别使用3 种粘接剂(Single Bond,SB;Clearfil SE Bond,SE;Clearfil S3 Bond,S3)制备粘接样本.粘接样本切割成粘接界面约为0.9 mm×0.9 mm的柱形小条,用微拉伸测力仪测试粘接强度(n=15).另外将粘接样本依次用6 mol/L盐酸、5%次氯酸钠和0.08 mg/ml透明质酸酶溶液处理,扫描电镜观察粘接界面形态.结果: 3 种粘接剂的牙本质粘接强度分别为:SB (35.50±6.40) MPa,SE (45.06±5.29) MPa,S3 (30.46±3.82) MPa,三者之间差异具有统计学意义(P<0.05).SB粘接界面的树脂突长约9～12 μm,部分样本出现混合层易与粘接剂层分离.SE形成的树脂突长约9～14 μm,无混合层与粘接剂层分离现象.使用S3后,树脂突稀疏,长约4～7 μm,混合层与粘接剂层结合紧密.结论:牙本质粘接剂的粘接界面形态与粘接强度存在一定的相关性.     

Micro-tensile and micro-shear bond strengths of current self-etch adhesives to enamel and dentin

PURPOSE: To evaluate the micro-tensile and micro-shear bond strengths of self-etch adhesives to enamel and dentin. METHODS: Extracted human molars were ground to expose flat enamel or dentin surfaces using wet #600 grit SiC paper. The enamel and dentin surfaces were assigned to four groups of four adhesives: three one-step self-etch adhesives (Clearfil S3 Bond; AQ Bond Plus, G-Bond) and a two-step self-etch adhesive (Clearfil SE Bond). Each of the adhesives were applied to the enamel or dentin surfaces in accordance with the manufacturers' instructions and restored with resin composite (Clearfil AP-X). The bonded teeth were then prepared for either micro-tensile or micro-shear bond strength tests. After storage in saline at 37 degrees C for 24 hours, specimens were stressed at a crosshead speed of 1 mm/minute. Mean bond strengths and modes of failure were analyzed using one-way ANOVA followed by Scheffe's F test and the Chi-square test, respectively, at a 95% level of confidence. RESULTS: SE Bond produced significantly higher values than the one-step adhesives in the micro-shear bond test to enamel (P< 0.05), while no significant differences were found among the adhesives in the micro-tensile bond test (P> 0.05). For dentin, SE Bond showed the highest bond strengths in both micro-tensile and micro-shear bond tests; values were significantly higher than both AQ Bond Plus and G-Bond (P< 0.05).     

Effect of dentin conditioners on the microtensile bond strength of a conventional and a self-etching primer adhesive system.

OBJECTIVE: To investigate the effect of different dentin treatments on the microtensile bond strength of a self-etching primer and a simplified, total-etch adhesive system. METHODS: Flat dentin surfaces were created on extracted human third molars. The surfaces were treated with one of the following conditioners: self-etching primer for 20 s (Clearfil SE Primer), 37% phosphoric acid for 15 s or 0.5 M EDTA for 30 s. Conditioned surfaces were then bonded with either Clearfil SE Bond or Single Bond followed by resin composite (Z250) build-ups constructed incrementally. Application of SE Primer was included when Clearfil SE Bond was used, after phosphoric acid and EDTA conditioning. After 24 h storage in water at 37 degrees C, the teeth were longitudinally sectioned across the bonded interface to produce beams with 1.0 mm2 of adhesive area, tested with the microtensile method at a rate of 0.5 mm/min. Data were analyzed using two-way ANOVA and Tukey's test. RESULTS: The highest bond strength mean was found for the combination SE Primer/Single Bond (58.5+/-20.8 MPa), followed by the EDTA/Clearfil SE Bond (47.8+/-15.1 MPa) and phosphoric acid/Single Bond (40.9+/-14.3 MPa). The remaining combinations showed statistically similar (p>0.05) tensile bond strength. SIGNIFICANCE: The bond performance of the adhesives tested was dependent on the dentin conditioner. Pre-treatment with a mild etchant such as 0.5 M EDTA improved the bond strength of Clearfil SE Bond. Single Bond performed better when a self-etching primer was used as the dentin conditioner, probably by preventing the formation of a defective zone at the base of the hybrid layer. Overall results indicate that higher bond strengths can be achieved by conditioning dentin with milder etchants, suggesting that deeper demineralization may prevent proper resin infiltration, hence compromising the bond.     

In vitro permeability of etch-and-rinse and self-etch adhesives used for immediate dentin sealing

To investigate the permeability of deep dentin following immediate sealing with different etch-and-rinse and self-etch adhesives (Single Bond 2, Adper Prompt L-Pop, Clearfil Protect Bond, Clearfil S3 Bond, G-Bond) and a dentin desensitizer (Gluma). Fluid-transport model was used to measure fluid conductance during and after application of adhesives. Polyvinylsiloxane impressions of bonded dentin were taken to monitor fluid transudation from the surface of the adhesives. The area and number of dentinal fluid droplets and/or blisters were calculated using image analysis. None of the adhesives were able to block fluid conductance completely. The fluid conductance values of the adhesives displayed the following statistical ranking (p<0.05): G-Bond≤Clearfil Protect Bond相似文献   

Comparison of enamel and dentin microshear bond strengths of a two-step self-etching priming system with five all-in-one systems

Data on the adhesive strength of new all-in-one adhesives are still relatively limited. This study compared the microshear bond strengths of five recent all-in-one self-etching priming systems (G-Bond, One-Up Bond-F Plus, Clearfil S3 Bond, Adper Prompt L-Pop and Go!) with a widely used two-step self-etching priming system (Clearfil SE Bond). Human molars were sectioned and finished with 600-grit SiC paper. Both enamel and dentin were bonded using adhesives with a 0.7 mm bonding diameter. Bond strengths were tested using a microshear bond test method at a crosshead speed of 1 mm/minute. The mean bond strengths and standard deviations were calculated and analyzed using ANOVA and the Tukey's HSD test. Results showed the two-step self-etching system had significantly higher bond strengths to dentin. However, for enamel bond strength, Clearfil SE Bond showed no statistical difference to G-Bond and Go!; however, all of the other materials were statistically lower. It is necessary to examine these new materials clinically to determine their efficacy.