表皮细胞生长因子及其受体在胎儿和成人肠道组织中的表达特征

目的探讨表皮细胞生长因子(EGF)及其受体(EGFR)在不同发育阶段人小肠组织中的表达特征及其可能的生物学意义。方法24例被测标本中包括胎儿(胎龄13～31周)的小肠组织18例，成人(16～54岁)的小肠组织6例。用病理学技术和免疫组织化学方法确定EGF及EGFR两种蛋白在胎儿、成人小肠组织中的定位以及表达量的变化规律．结果EGF及EGFR在不同胎龄的胎儿和成人小肠组织中均有阳性表达。随着胎儿生长发育，小肠组织中EGF和EGFR的蛋白含量逐渐增加，这一变化趋势一直保持到成人小肠组织中。其中EGF主要存在于小肠黏膜上皮细胞、黏膜下层的血管内皮细胞内和浆膜上皮细胞的胞浆和胞外基质中，EGFR则分布于这些细胞的细胞膜上。结论EGF及EGFR在不同发育阶段的小肠组织中呈阳性表达，这种细胞因子可能以自分泌或旁分泌方式调节人肠道的生长发育、结构和功能的维持，并可能在小肠损伤后的修复中起重要作用。     

碱性成纤维细胞生长因子和表皮生长因子对骨骼肌源性干细胞的促增殖效应

目的观察碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）和表皮生长因子（epidermal growth factor，EGF）单独及联合应用对骨骼肌源性干细胞（muscle derived stem cells，MDSCs）生长的影响。方法取出生24h内的昆明小鼠15只，采用连续预贴壁法从小鼠后肢肌分离培养MDSCs，用含2％胎牛血清的DMEM培养基促进其向骨骼肌细胞分化。取原代MDSCs及MDSCs分化细胞，采用免疫细胞化学染色检测干细胞标志Sca-1和骨骼肌细胞标志α-Sarcomeric肌动蛋白的表达。HE染色观察细胞肌管形成。MTT比色法检测不同浓度（6．25、12．50、25．00、50．00、100．00ng／ml）的bFGF和EGF单独应用96h对MDSCs增殖的影响以及二者（100．00ng／ml）联合作用24、48、72和96h对MDSCs增殖的影响。结果从新生小鼠后肢肌成功分离培养MDSCs，免疫细胞化学染色90％以上的MDSCs呈Sca-1阳性，分化形成的肌管呈α—Sarcomeric肌动蛋白阳性。HE染色可见肌管形成。bFGF、EGF对MDSCs的促增殖效应随浓度的增加而增加。与阴性对照组比较，bFGF于12．50ng／ml出现促增殖效应（P〈0．05）；25．00ng／ml组与12．50ng／ml组比较，作用提高（P〈0．01）；50．00、100．00ng／ml组较25．00ng／ml组无明显提升（P〉0．05）；EGF的作用与bFGF类似，但于50．00ng／ml时趋于饱和。与阴性对照组比较，EGF于72h、bFGF于96h表现促增殖效应（P〈0．01），而二者联合应用于24h即表现促增殖效应（P〈0．01），并于48、72和96h增殖效应均较单独应用显著提高（P〈0．05）。结论bFGF和EGF都能促进MDSCs的增殖，联合作用更快、更强。     

表皮细胞生长因子和碱性成纤维细胞生长因子在不同发育阶段大鼠皮肤表达特征的比较性研究

目的 研究表皮细胞生长因子（EGF）和碱性成纤维细胞生长因子（bFGF)在胚胎、新生及成年三个不同发育阶段大鼠皮肤的表达特征及其可能的生物学意义。方法 取胚胎、新生和成年大鼠皮肤,经4%多聚甲醛固定、包埋与切片后,用SP免疫组织化学法研究EGF和bFGF的定位与表达特征。结果 EGF的阳性表达可见于胚胎、新生及成年三个发育阶段大鼠皮肤,主要位于表皮棘细胞,真皮成纤维细胞、毛囊上皮细胞和血管内皮细胞胞浆,bFGF在新生和成年大鼠表皮及真皮呈强阳性表达,但在胎鼠皮肤则为弱阳性乃至阴性,结论 EGF在不同发育阶段大鼠皮肤呈强阳性表达,表明其对上皮的发生、表型维持以及损伤后的修复十分重要。而大鼠胚胎皮肤bFGF低表达或缺乏的结果表明它可能是动物胚胎创伤后无瘢痕愈合的原因之一。     

汉防己甲素对先天性膈疝大鼠模型胎仔肺内表皮生长因子及其受体的影响和意义

目的 探讨中药成分汉防己甲素（tetrandrine,TET）对大鼠先天性膈疝（congenital diaphragmatic hernia,CDH）模型胎仔肺内表皮生长因子（epidermal growth factor,EGF）及其受体（epidermal growth factor receptor,EGFR）的影响和意义。方法 实验组20只雌性SD大鼠于孕9．5d灌胃给予除草醚115mg／只;对照组4只灌胃给等量食用油。孕18．5d,实验组根据给药不同随机分为生理盐水组（normal solution group,NS组）、地塞米松组（dexamethasone group,Dex组）、汉防己甲素组（tetrandringe group,TET组）、地塞米松＋汉防己甲素组（D＋T组）,每组5只。孕21．5d对所有孕鼠行刮宫产,取出胎鼠两侧肺组织行大体观察、HE染色、EGF、EGFR免疫组织化学染色和图像分析。结果 正常对照组共产胎仔36只,无隔疝形成。实验组共产胎仔137只,CDH形成64只,致畸率46．7％。在Dex组、TET组和T＋D组,肺组织处于原始肺泡期或进一步成熟。EGF在NS组、Dex组、TET组、D＋T组及对照组的表达量依次递减（P〈0．05）,而EGFR在NS组、Dex组、TET组、D＋T组和对照组的表达量依次递增（P〈0．05）。结论 TET可使CDH胎鼠肺内EGF表达高峰提前,使EGFR表达上调,TET和Dex联合作用对促进EGF表达高峰提前及EGFR表达上调具有明显的协同效应。TET可以通过调节EGF及EGFR在肺内的表达而实现其促进肺组织细胞发育分化和改善肺血管构型的效应。     

Expression of Epidermal Growth Factor Receptors in Human Breast Cancer: Mass Versus Ligand Binding Capacity

Abstract: Thirty percent of lymph node-negative women with primary breast cancers are at high risk for early recurrence of metastatic disease and diminished survival. Identification of these high-risk patients is a long-term objective of our laboatory, and the epidermal growth factor receptor (EGFR) was investigated as a prognostic factor, EGFR was measured by a ligand binding assay developed in house, in the competition mode that served as the "gold standard" for assessing receptor content and activity. In contrast, measurements of mass (content) were performed by an enzyme immunoassay (EIA) from Ciba Corning Diagnostics (Alameda, CA) and by an enzyme-linked immunosorbent assay (ELISA) from Oncogene Science (Uniondale, NY). A total of 78 breast carcinomas were examined. The median binding capacity measured with [¹²⁵I]EGF was 13 fmol/mg membrane protein (range 0–981), that measured by EIA was 8 fmol/mg (range 1–125), while EGFR measured by ELISA was 135 (range 0–751). Distribution of EGFR did not appear to vary as a function of patient age. Neither the results from EIA nor those from ELISA correlated with those obtained by the ligand binding assay. However, there was a good correlation of results obtained by the two antibody-based assays despite the fact that the calibration of standards was considerably different. These data suggest that EGFR should be measured by ligand binding assay for clinical studies; mass assays based on antibody reagents will require further development.     

转基因成纤维细胞向创面持续投递新型分泌型表皮细胞生长因子

目的 探索通过转基因成纤维细胞向创面持续投递表皮细胞生长因子（ＥＧＦ）促进创面愈合的新方法。方法 构建新型分泌型人ＥＧＦｃＤＮＡ并转染人成纤维细胞株ＫＭＳＴ６。将经照射的转基因细胞移植于裸鼠全厚创面。结果 稳定转染的细胞克隆可分泌有活性的ＥＧＦ。移植后可在伤口组织中检测到人ＥＧＦ,其含量缓慢下降,但至少可持续７天。结论 一次性移植少量转基因细胞可在创面愈合早期这一关键阶段持续向创面释放ＥＧＦ。     

表皮细胞生长因子通过诱导皮肤干细胞分化加速受创表皮再生的研究

目的：从皮肤干细胞增殖分化角度研究表皮细胞生长因子（EGF）加速受创皮肤再生的机制。方法：8例经EGF治疗创面于治疗后8天及14天对创面边缘部进行活检取材。用常规病理与SP免疫组织化学法研究β1整合素、角蛋白19（K19）、角蛋白14（K14）以及角蛋白10（K10）在不同修复阶段修复表皮的表达特征。另选研究β1整合素、角蛋白19（K19）、角蛋白14（K14）以及角蛋白10（K10）在不同修复阶段修复表皮的表达特征。另选7例同期未经EGF治疗创面为对照。结果：常规病理学检查与对照创面相比，EGF治疗创面后8天可见表皮层增厚，表皮脚增粗，治疗后14天以上特征更为明显。SP免疫组织化学对β1整合素与K19染色表明，EGF治疗8天的创面表皮干细胞与短暂扩充细胞不仅数量多，而且体积增大，治疗后14天创面面生表皮的棘细胞层中有干细胞岛出现。对照治疗后8天与14天创面除有一定数量的β1整合素与K19阳性染色细胞外，未见干细胞岛出现，在EGF治疗和对照治疗创面，治疗后8天及14天，K16、K10表达均见于再生上皮的表层，即那些已失去增殖能力与终末分化细胞。结论：EGF促进损伤皮肤再生的主要机制之一可能与它能诱导皮肤干细胞快速定向分化有关。     

Clinical significance of EGF and EGFR expression changes in cryptorchid boys

Aim: To explore the changes of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) expressions in cryptorchid children and their clinical significance. Methods: The level of serum EGF was measured by radioimmunoassay (RIA) and the expression of EGFR by immunohistochemistry. Results: (1) The level of serum EGF was significantly lower in cryptorchid children than in normal subjects at age group of 59 years (P<0.01) and 10-14 years (P<0.01), (2) The level of EGF was significantly lower in boys with impalpable testis than in those with extracanalicular and intracanalicular testes (P<0.01), (3) The serum EGF level increased significantly 6 months after orchiopexy (P<0.05), (4) The EGFR expression in testicular Leydig's cells was lower in 2-4 year-old boys than in those over 5 years (P<0.05) and (5) the EGFR expression was less positive in the impalpable group and the intracanalicular group than that of the extracanalicular group (P<0.01). Conclusion: The EGF and EGFR expr essions may co     

转化生长因子调控表皮生长因子受体在雄激素非依赖型前列腺癌细胞中表达的意义

目的 :探讨转化生长因子 (TGF)α对前列腺癌雄激素非依赖型细胞系中表皮生长因子受体 (EGFR)表达的调控。　方法 :采用逆转录 多聚酶链式反应和Western印迹法对TGFα刺激前列腺癌雄激素非依赖型细胞系PC3、ARCaP后EGFRmRNA表达及其蛋白水平进行定量分析。　结果 :TGFα引起PC3、ARCaP的EGFRmRNA表达升高 ,分别为 5 .0 1± 0 .4 5和 9.0 5± 0 .6 3,明显高于对照组 (P <0 .0 5 )。PC3细胞系TGFα治疗后EGFR蛋白水平为 2 .2 8±0 .5 3,明显高于对照组 (P <0 .0 5 ) ;而ARCaP细胞系TGFα治疗后EGFR仅为 1 .2 4± 0 .2 2 ,和对照组比较无差别 (P >0 .0 5 )。　结论 :TGFα可以明显提高前列腺癌细胞的EGFR表达量 ;TGFα EGFR自分泌环参与雄激素非依赖型前列腺癌的形成     

INHIBITORY EFFECTS OF BESTRABUCIL, A CONJUGATE OF CHLORAMBUCIL AND ESTRADIOL, ON THE PRODUCTION OF ANDROGEN-INDUCED GROWTH FACTOR(S) BY SHIONOGI CARCINOMA 115 CELLS

It is known that diffusible trophic factors play an important role in both the normal and cancerous growth regulatory processes of hormone-responsive cells such as are found in the prostate and mammary glands. Consequently, it is important to identify whether the production of such growth factors is affected by administration of therapeutic agents. We examined the effect of bestrabucil, a benzoate of an estradiol-chlorambucil conjugate, on the production of growth factor(s) by Shionogi carcinoma 115 (SC-115) cells, an androgen-responsive cultured cancer cell line. We then investigated whether the inhibitory effect found was specific to bestrabucil, or if it was also produced by a mixture of the 2 compounds, estradiol and chlorambucil. Bioassay employing BALB/3T3 cells demonstrated the presence of two kinds of growth factor in the conditioned medium obtained by culturing SC-115 cells in medium containing 10-⁸ M testosterone; these factors could be separated by heparin-sepharose column chromatography using 0.5 M NaCI and 1.1 M NaCI. When the SC-115 cells were cultured in medium containing bestrabucil, at a concentration of 10⁻⁵ M, no growth factor activity was detected in the fraction eluted from the heparin-sepharose column by 1.1 M NaCI. At bestrabucil concentrations of 10⁵-10⁷ M, concentration-dependent inhibition of growth factor production by SC-115 cells could be demonstrated by ³H-thymidine uptake assay. However, this inhibitory effect could not be demonstrated using only a mixture of estradiol and chlorambucil. The finding that bestrabucil specifically inhibits the production of growth factor(s) may help in the development of a new class of drugs which will be clinically useful in treating hormone-responsive cancer.     

重组人表皮细胞生长因子在慢性溃疡创面中的应用

目的　观察重组人表皮细胞生长因子 (rh EGF)对慢性溃疡创面愈合的影响。方法　 1999年 10月～ 2 0 0 1年 1月在局部清创后随机对 2 6例患者的慢性溃疡创面 (3cm× 3cm～ 5 cm× 8cm )作为治疗组 ,用rh EGF喷雾剂以 40 0 U / 10 cm2 进行局部喷涂 ,无菌敷料覆盖 ;同期随机对另 2 6例慢性溃疡创面 (2 .5 cm×3.0 cm～ 4.0 cm× 6 .5 cm )用 0 .9%生理盐水进行局部湿敷作为对照组 ,观察创面愈合时间、创面局部及全身反应情况。结果　治疗组中创面不适感消失早 ,脓性分泌物少 ,肉芽组织生长快 ,无过度增生现象。溃疡创面愈合时间治疗组较对照组缩短 7～ 10天 ,经统计学处理 ,有显著性差异 (P<0 .0 1)。结论　 rh EGF有明显促进慢性溃疡创面愈合的作用 ,且愈合时间短、质量好     

Androgenic and antiandrogenic control on epidermal growth factor,epidermal growth factor receptor,and androgen receptor expression in human prostate cancer cell line LNCaP

Both androgen and antiandrogen treatments enhance the proliferation rate of the hormone-dependent prostate cancer cell line LNCaP, expressing a mutated androgen receptor (AR). We studied the modification of the expression of epidermal growth factor (EGF), of its receptor (EGF-R), and of androgen receptor (AR) in the LNCaP cell line, under basal conditions and during androgen (R1881) and antiandrogen hydroxy-flutamide (OH-FLU) treatment. After prolonged R1881 administration, a marked increase of EGF release was observed, completely blocked by the addition of OH-FLU. The Scatchard plot analysis of EGF-R binding revealed two classes of binding sites with high and low affinity. The administration of OH-FLU alone or combined with R1881 did not modify the affinity constants, while the low-affinity component disappeared after androgen administration. Both androgen and antiandrogen administration led to a significant increase of the EGF-R high-affinity component. AR mRNA and protein levels were downregulated by R1881 treatment. Following OH-FLU administration, AR mRNA was slightly downregulated, and there was not a strict parallelism between AR mRNA levels and AR binding capacity. When combined with R1881, OH-FLU partially counteracted the androgen-induced AR downregulation. Our data show that EGF-R binding capacity is the only parameter constantly raised in cell proliferation with respect to quiescent cells, and highlights the nonunivocal action of OH-FLU on androgen-induced effects.     

神经生长因子调控烧伤创面的实验研究

目的 研究神经生长因子（ＮＧＦ）在烧伤创面愈合的作用,探索烧伤创面愈合机制。方法２０ｋｇ小白家猪６只为实验对象。用控温控压电烫仪在每只猪背部制成２４个直径为２．５ｃｍ的圆形深Ⅱ度烧伤创面。分为四组,分别为创面局部应用１、２．５和５μｇ／ｍｌ的ＮＧＦ实验组和不含ＮＧＦ的生理盐水对照组。在用药后３、５和９天分别取创面组织进行表皮生长因子（ＥＧＦ）受体,ＥＧＦ,ＮＧＦ受体,ＮＧＦ,ＣＤ６８,ＣＤ３免疫组     

Changes in epidermal growth factor receptor expression in human bladder cancer cell lines following interferon-alpha treatment

PURPOSE: We examined the regulation of epidermal growth factor (EGF) receptor (EGFR) expression in human bladder cancer cell lines by interferon-alpha (IFN-alpha), the ability of IFN-alpha to inhibit cell proliferation and the sensitivity of IFN-alpha pretreated cells to EGF. MATERIALS AND METHODS: Cell proliferation was determined using crystal violet colorimetric and clonogenic assays. EGFR expression was measured by flow cytometry using specific antibody or ligand binding approaches. RESULTS: After IFN-alpha (100 IU/ml) treatment cell surface EGFR expression was upregulated in 6 of 11 and down-regulated in 2 of 11 bladder cancer cell lines. The over expression of cell surface EGFR peaked within 48 to 96 hours and increased by 35% to 241% in individual cell lines. High level cell surface EGFR correlated with intracellular EGFR expression. Cell growth inhibition by IFN-alpha coexisted with EGFR over expression in the 6 lines. IFN-alpha treated cells remained sensitive to EGF treatment. CONCLUSIONS: IFN-alpha transiently up-regulates EGFR expression and inhibits in vitro growth in some human bladder cancer cells. IFN-alpha does not prevent EGFR from binding EGF or signal transduction via the EGF-EGFR pathway. This may have clinical implications for improving treatment based on EGFR targeting in select patients with bladder cancer.     

URINARY EPIDERMAL GROWTH FACTOR IN DIFFERENT RENAL CONDITIONS IN CHILDREN

Several studies have demonstrated the important role of growth factors, particularly epidermal growth factor (EGF) and transforming growth factor α (TGFα), in cellular growth after renal damage. EGF is mainly synthesized by the kidney. Many studies indicate that urinary EGF concentration significantly decreases in patients with acute and chronic renal failure. In this study we determined urinary EGF concentrations in children with renal and/or urological pathologies. We investigated 38 patients, 17 males and 21 females, of 3.34 ± 2.96 years (mean ± standard deviation), who were followed in the Nephrologic Unit of the Pediatric Department of the University of Verona for recurrent urinary tract infections: seven of these had vesicoureteric reflux and 4 had hypodysplasia. The results were compared with those from a healthy age-matched group of 44 children. In all patients, we assessed renal function including an examination of the urine with a microbiological evaluation. Moreover, a renal ultrasound and a voiding cystourethrogram were performed. Urinary EGF was measured by a radioimmunoassay, using polyclonal goat antibodies. In all patients, laboratory parameters were within the normal range. In 34 patients the renal ultrasound was negative and in 4 cases structural alterations of the renal parenchyma were found. Voiding cystourethrography detected 7 instances of vesicoureteric reflux. In controls 10°, 50°, 90° percentile uEGF values were 7.3, 19 and 40.4μg/L, respectively. Mean urinary EGF values were 22.22 ± 16 μg/L. Urinary EGF values were 54 ± 35.2 μg/L in patients with recurrent urinary tract infections and without urinary malformations, 81 ± 29.37 μg/L in patients with vesicoureteric reflux and 22.30 ± 22.90μg/L in patients with hypodysplasia, respectively. There was a statistical significant difference between controls and groups A (p < 0.001) and B (p < 0.001) respectively, while the difference between group C and controls wasn't significant (p = 0.044). Results are reported in Figure . We believe that our results could be helpful for further studies on pathophysiology of growth factors in different renal conditions of children.

URINARY EPIDERMAL GROWTH FACTOR IN DIFFERENT RENAL CONDITIONS IN CHILDREN

All authors

V. Fanos, C. Pizzini, M. Mussap, D. Benini & M. Plebani

https://doi.org/10.1081/JDI-100104742

Published online:

07 July 2009

Figure 1. Urinary epidermal growth factor concentrations (uEGF) in the three groups considered (data are expressed as mean values ± standard deviation) compared with the line of the 90° percentile value calculated in the control group. Unpaired t-test: Group A vs. controls, p < 0.001. Group B vs. controls, p < 0.001. Group C vs. controls, n.s. (p = 0.487). Group A vs. Group B, p = 0.044.     

16.

表皮细胞生长因子与碱性成纤维细胞生长因子促进创面修复效应的比较性研究   总被引：57，自引：10，他引：47  

傅小兵  孙同柱  王亚平  杨银辉  蒋礼先  顾小曼  常国友  盛志勇 《中国修复重建外科杂志》1999,13(5):278-282

目的 采用小型猪背部创伤模型,定量研究与比较重组人表皮细胞生长因子与重组人碱性成纤维细胞生长因子的促修复效应与作用机制。方法 以创伤模型制作器切割小型猪背部皮肤,造成典型全层皮肤损伤。将１６只小型猪背部的１６０个创面分成两大组,分别以三种不同剂量的ｒｈＥＧＦ（５０,１０及０．５μｇ／创面）和ｒｈＦＧＦ（１５０、９０及３０Ｕ／ｃｍ＾２创面）治疗,每组同时各设溶媒为阴性对照。以伤腔容积与创面愈合面积,     

17.

膀胱移行细胞癌表皮生长因子及其受体表达和意义     

任宝明  李静  张天宝 《现代泌尿外科杂志》2003,8(2):90-91,108

目的 探讨表皮生长因子(EGF)、EGF受体(EGFR)在膀胱移行细胞癌(BTCC)中的表达及意义。方法 用免疫组化ABC法对56例BTCC标本和20例癌旁组织EGF、EGFR进行研究。结果 20例癌旁正常膀胱组织中EGF，EGFR几乎不表达；56例ETCC标本中EGF，EGFR表达明显，与正常组比较有显著性差异(P＜0．01)，不同分期和分级BTCC中的阳性表达率均存在显著性差异(P＜0．05)。结论 EGF，EGFR的检测可以作为判断BTCC预后的一个指标。     

18.

重组人表皮生长因子软膏对烧伤创面修复的促进作用   总被引：38，自引：2，他引：36  

王世岭  马建丽  柴家科  周亮  廖镇江  黄跃生  利天增  李利根  付小兵 《中国修复重建外科杂志》2002,16(3):173-176

目的　探讨重组人表皮生长因子 (rh EGF)软膏治疗烧伤的有效浓度 ,并观察对创面愈合的促进作用。方法　将 12 0例烧伤患者分为两部分 ,第一部分选择 15例浅 °烧伤患者 ,采用开放实验 ,分三组每组 5例 ,进行三种不同浓度 rh EGF软膏 (0 .5 μg/ g,10 μg/ g,5 0 μg/ g)的疗效比较 ,确定临床使用浓度。第二部分 ,选择浅 °和深 °烧伤患者10 5例为试验对象 ,在第一部分研究的基础上选取最适药浓度 ,进行多中心随机双盲实验 ,所有患者均采用自身对照。以创面愈合为指标 ,判断创面愈合时间 ,并观察创面动态愈合率及不良反应。结果　第一部分患者 10 μg/ g和 5 0 μg/ g组的创面愈合时间差异不显著但较 0 .5 μg/ g组明显缩短 ,有统计学意义 (P<0 .0 1)。第二部分选用 10 μg/ g浓度的 rh EGF软膏与水溶性软膏基质进行随机双盲研究。浅 °创面用药组愈合时间为 (8.39± 2 .2 5 )天 ,空白对照组为 (9.5 2± 2 .5 6 )天 ,组间比较具有统计学意义 (P<0 .0 1) ;用药组不同时间内的愈合率较对照组明显提高 ;深 °创面愈合时间用药组、对照组分别为 (16 .80± 2 .99)天 ,(18.2 7± 3.17)天 ,组间比较有统计学意义 (P<0 .0 1)。结论　rh EGF软膏对烧伤创面有明显的促进修复作用 ,且 10 μg/ g的浓度较为理想     

19.

Characterization of insulin-like growth factor I and epidermal growth factor receptors in meningioma   总被引：1，自引：0，他引：1  

M Kurihara  Y Tokunaga  K Tsutsumi  T Kawaguchi  K Shigematsu  M Niwa  K Mori 《Journal of neurosurgery》1989,71(4):538-544

Receptors for insulin-like growth factor I (IGF-I) and epidermal growth factor (EGF) were localized and characterized in eight samples of human meningioma (four fibrous, two meningothelial, and two angioblastic types), using quantitative autoradiographic techniques. Effects of both growth factors on deoxyribonucleic acid (DNA) synthesis in the cultured meningioma cells were examined. High numbers of specific binding sites for both IGF-I and EGF were homogeneously present in tissue sections derived from fibrous and meningothelial types of meningiomas, whereas binding sites for these growth factors were not detectable in adjacent leptomeninges. While relatively large numbers of IGF-I binding sites were located in the wall of the intratumoral vasculature, the number of binding sites in the stromal component was lower in angioblastic-type meningiomas, including a low number of EGF binding sites detected only in the stromal portion. Scatchard analysis revealed the presence of a single class of high-affinity binding sites for both IGF-I and EGF in the meningiomas examined (dissociation constant (Kd) = 0.6 to 2.9 nM, and the maximum number of binding sites (Bmax) = 16 to 80 fmol/mg for IGF-I; and Kd = 0.6 to 4.0 nM, Bmax = 3 to 39 fmol/mg for EGF). Both growth factors increased the synthesis of DNA, in a dose-dependent manner, as measured by 3H-thymidine incorporation. The combination of IGF-I and EGF synergistically stimulated the synthesis of DNA, and the effects seen with 10% fetal bovine serum could be reproduced at a concentration of 10(-10) M. These observations can be interpreted to mean that both IGF-I and EGF may be involved in the growth modulation of meningiomas, possibly through paracrine or autocrine mechanisms.     

20.

Characterization, quantification, and potential clinical value of the epidermal growth factor receptor in head and neck squamous cell carcinomas   总被引：7，自引：0，他引：7  

J Santini  J L Formento  M Francoual  G Milano  M Schneider  O Dassonville  F Demard 《Head & neck》1991,13(2):132-139

Epidermal growth factor (EGF) stimulates the growth of several types of epithelial tissues and possesses a strong mitogenic activity that is mediated through its cell surface receptor (EGFR). The aim of this study was to characterize EGFR and measure its levels in head and neck tumors biopsies (70 patients); use of a simplified competition technique with a radiolabeled ligand allowed evaluation of functional EGFR. Five samples (4 tumors and 1 control) were used to characterize EGF binding. Graphic representation identified a single family of binding sites. Kd values revealed high affinity for EGF binding: mean Kd, 0.156 +/- 0.108 nM (0.095-0.347 nM). EGF-binding characteristics (Kd) were similar in nontumoral tissue samples (controls) and in tumor material. In 59 of 60 cases, EGFR levels were higher in the tumor than in the corresponding controls. A significant correlation was found between EGFR levels and tumor size and stage. Controls exhibited a trend toward higher EGFR levels in elevated sizes and stages. According to a cutoff EGFR value of 100 fmol/mg protein, which separated all controls from tumors, EGFR-positive tumors (greater than 100 fmol/mg protein) had a greater probability of complete response to chemotherapy than EGFR-negative tumors; other tumor characteristics, such as the degree of tumoral differentiation, tumor size, or stage, were unable to operate such a discrimination in the response to chemotherapy. EGFR may thus be an interesting biological marker for head and neck cancer.     

表皮细胞生长因子与碱性成纤维细胞生长因子促进创面修复效应的比较性研究

目的 采用小型猪背部创伤模型,定量研究与比较重组人表皮细胞生长因子与重组人碱性成纤维细胞生长因子的促修复效应与作用机制。方法 以创伤模型制作器切割小型猪背部皮肤,造成典型全层皮肤损伤。将１６只小型猪背部的１６０个创面分成两大组,分别以三种不同剂量的ｒｈＥＧＦ（５０,１０及０．５μｇ／创面）和ｒｈＦＧＦ（１５０、９０及３０Ｕ／ｃｍ＾２创面）治疗,每组同时各设溶媒为阴性对照。以伤腔容积与创面愈合面积,     

膀胱移行细胞癌表皮生长因子及其受体表达和意义

目的 探讨表皮生长因子(EGF)、EGF受体(EGFR)在膀胱移行细胞癌(BTCC)中的表达及意义。方法 用免疫组化ABC法对56例BTCC标本和20例癌旁组织EGF、EGFR进行研究。结果 20例癌旁正常膀胱组织中EGF，EGFR几乎不表达；56例ETCC标本中EGF，EGFR表达明显，与正常组比较有显著性差异(P＜0．01)，不同分期和分级BTCC中的阳性表达率均存在显著性差异(P＜0．05)。结论 EGF，EGFR的检测可以作为判断BTCC预后的一个指标。     

重组人表皮生长因子软膏对烧伤创面修复的促进作用

目的　探讨重组人表皮生长因子 (rh EGF)软膏治疗烧伤的有效浓度 ,并观察对创面愈合的促进作用。方法　将 12 0例烧伤患者分为两部分 ,第一部分选择 15例浅 °烧伤患者 ,采用开放实验 ,分三组每组 5例 ,进行三种不同浓度 rh EGF软膏 (0 .5 μg/ g,10 μg/ g,5 0 μg/ g)的疗效比较 ,确定临床使用浓度。第二部分 ,选择浅 °和深 °烧伤患者10 5例为试验对象 ,在第一部分研究的基础上选取最适药浓度 ,进行多中心随机双盲实验 ,所有患者均采用自身对照。以创面愈合为指标 ,判断创面愈合时间 ,并观察创面动态愈合率及不良反应。结果　第一部分患者 10 μg/ g和 5 0 μg/ g组的创面愈合时间差异不显著但较 0 .5 μg/ g组明显缩短 ,有统计学意义 (P<0 .0 1)。第二部分选用 10 μg/ g浓度的 rh EGF软膏与水溶性软膏基质进行随机双盲研究。浅 °创面用药组愈合时间为 (8.39± 2 .2 5 )天 ,空白对照组为 (9.5 2± 2 .5 6 )天 ,组间比较具有统计学意义 (P<0 .0 1) ;用药组不同时间内的愈合率较对照组明显提高 ;深 °创面愈合时间用药组、对照组分别为 (16 .80± 2 .99)天 ,(18.2 7± 3.17)天 ,组间比较有统计学意义 (P<0 .0 1)。结论　rh EGF软膏对烧伤创面有明显的促进修复作用 ,且 10 μg/ g的浓度较为理想     

Characterization of insulin-like growth factor I and epidermal growth factor receptors in meningioma

Receptors for insulin-like growth factor I (IGF-I) and epidermal growth factor (EGF) were localized and characterized in eight samples of human meningioma (four fibrous, two meningothelial, and two angioblastic types), using quantitative autoradiographic techniques. Effects of both growth factors on deoxyribonucleic acid (DNA) synthesis in the cultured meningioma cells were examined. High numbers of specific binding sites for both IGF-I and EGF were homogeneously present in tissue sections derived from fibrous and meningothelial types of meningiomas, whereas binding sites for these growth factors were not detectable in adjacent leptomeninges. While relatively large numbers of IGF-I binding sites were located in the wall of the intratumoral vasculature, the number of binding sites in the stromal component was lower in angioblastic-type meningiomas, including a low number of EGF binding sites detected only in the stromal portion. Scatchard analysis revealed the presence of a single class of high-affinity binding sites for both IGF-I and EGF in the meningiomas examined (dissociation constant (Kd) = 0.6 to 2.9 nM, and the maximum number of binding sites (Bmax) = 16 to 80 fmol/mg for IGF-I; and Kd = 0.6 to 4.0 nM, Bmax = 3 to 39 fmol/mg for EGF). Both growth factors increased the synthesis of DNA, in a dose-dependent manner, as measured by 3H-thymidine incorporation. The combination of IGF-I and EGF synergistically stimulated the synthesis of DNA, and the effects seen with 10% fetal bovine serum could be reproduced at a concentration of 10(-10) M. These observations can be interpreted to mean that both IGF-I and EGF may be involved in the growth modulation of meningiomas, possibly through paracrine or autocrine mechanisms.     

Characterization, quantification, and potential clinical value of the epidermal growth factor receptor in head and neck squamous cell carcinomas

Epidermal growth factor (EGF) stimulates the growth of several types of epithelial tissues and possesses a strong mitogenic activity that is mediated through its cell surface receptor (EGFR). The aim of this study was to characterize EGFR and measure its levels in head and neck tumors biopsies (70 patients); use of a simplified competition technique with a radiolabeled ligand allowed evaluation of functional EGFR. Five samples (4 tumors and 1 control) were used to characterize EGF binding. Graphic representation identified a single family of binding sites. Kd values revealed high affinity for EGF binding: mean Kd, 0.156 +/- 0.108 nM (0.095-0.347 nM). EGF-binding characteristics (Kd) were similar in nontumoral tissue samples (controls) and in tumor material. In 59 of 60 cases, EGFR levels were higher in the tumor than in the corresponding controls. A significant correlation was found between EGFR levels and tumor size and stage. Controls exhibited a trend toward higher EGFR levels in elevated sizes and stages. According to a cutoff EGFR value of 100 fmol/mg protein, which separated all controls from tumors, EGFR-positive tumors (greater than 100 fmol/mg protein) had a greater probability of complete response to chemotherapy than EGFR-negative tumors; other tumor characteristics, such as the degree of tumoral differentiation, tumor size, or stage, were unable to operate such a discrimination in the response to chemotherapy. EGFR may thus be an interesting biological marker for head and neck cancer.