枸杞籽油对慢性脑低灌注损伤大鼠的保护作用

目的 观察枸杞籽油对慢性脑低灌注损伤大鼠空间记忆及皮层自由基代谢的影响.方法 SD大鼠50只,随机分为假手术组、模型组及枸杞籽油高、中、低剂量组,除假手术组外,其余各组经双侧颈动脉结扎建立慢性脑低灌注损伤模型,枸杞籽油80、40、20 μL· kg-1灌胃60 d后,进行Morris水迷宫实验,检测其空间记忆能力,水迷宫实验结束后,取大鼠海马及皮层组织测其T-AOC活力及丙二醛(MDA)含量.结果 与假手术组相比,模型组出现明显的学习记忆障碍,治疗组较模型组学习记忆障碍有明显改善.与模型组相比,治疗组脑组织内T-AOC活性显著升高,MDA含量明显降低.结论 枸杞籽油能明显减轻大鼠慢性脑缺血所致认知功能障碍,可能与其清除自由基作用有关.     

PKC和MMPs在枸杞籽油抗动脉粥样硬化中的作用和机制

目的 研究枸杞籽油抗动脉粥样硬化的功效及其可能的作用机制.方法 高脂膳食制备家兔动脉粥样硬化实验模型,观测其血浆中TC、TG、LDL-C、HDL-C、ApoA、ApoB的变化;56 d后,测定血清SOD、GsH-Px、T-AOC活性和MDA的含量;石蜡切片进行病理学检测;免疫印迹检测PKC、MMP-2、MMP-9在血管中的表达.结果 枸杞籽油可增加实验家兔血浆中HDL-C、ApoA的含量,降低血浆中TC、TG、LDL-C、ApoB的含量,增强血清中SOD、GSH-PX、T-AOC的活性,降低血清中MDA的含量;降低PKC、MMP-2、MMP-9在血管中的表达.结论 枸杞籽油具有显著的抗动脉粥样硬化效应.其机制可能是通过PKC途径,减少MMPs的分泌、抑制SMC的增生、分泌胶原、降血脂和抗氧化等.     

不同方法制备枸杞籽油微乳的比较研究

目的:优选枸杞籽油微乳的处方组成,比较枸杞籽油微乳的3种制备方法。方法:以枸杞籽油自身作为油相,利用外观、乳滴粒径、三元相图和伪三元相图等评价指标,优选枸杞籽油微乳的制剂处方;以乳滴粒径和粒度分布为指标,比较超声乳化法、高压乳匀法和自乳化法制备枸杞籽油微乳的不同特点。结果:优选的处方为1%枸杞籽油、1%吐温80、2%甘油;超声乳化法的工艺参数为超声功率480W,工作-间歇时间为10s-5s,超声时间25min,制得微乳的平均粒径为(74.6±45.3)nm(多分散系数(P.I)=0.368);高压乳匀法的工艺参数为均质压力500bar循环3次,制得微乳平均粒径为(69.0±31.5)nm(P.I=0.378);在相同处方比例下,自乳化法无法自发形成微乳,而当枸杞籽油:吐温80:甘油=1:7:2时,可自发形成微乳。结论:不同方法均能制得粒径小而均匀、外观透明有乳光的枸杞籽油微乳,但各法适用范围和特点不同。     

播娘蒿籽油对小鼠学习记忆能力的影响

目的探讨播娘蒿籽油对小鼠记忆获得障碍模型的影响。方法以跳台法和避暗法测试小鼠学习记忆的能力,用比色法测定小鼠脑组织胆碱乙酰转移酶(ChAT)、乙酰胆碱酯酶(AchE)活性,用碱性羟胺法测定乙酰胆碱(Ach)含量。结果播娘蒿籽油可减少小鼠跳台和避暗错误次数,并能增加小鼠脑组织乙酰胆碱(ACh)含量和胆碱乙酰转移酶(ChAT)活性,同时降低脑组织乙酰胆碱酯酶(AchE)活性,有助于小鼠空间学习记忆能力的改善。结论播娘蒿籽油可以显著促进小鼠记忆。     

梁氏情境应激箱对小鼠抑郁样行为的诱导作用

目的观察和比较梁氏情境应激箱对小鼠抑郁样行为的诱导作用,对其表观效度进行评价。方法将小鼠放入4种不同应激环境中15 min,随后采用饮糖偏爱实验、社交行为实验、新奇抑制摄食实验以及强迫游泳实验,评价4种情境应激环境对小鼠抑郁样行为相关指标的影响。结果①与饲养鼠笼、正方形箱和三角形箱3种情境应激环境相比较,梁氏情境应激箱处理显著性降低ICR小鼠的饮糖偏爱率。此外,间隔3 d重复3次给予梁氏情境应激刺激仍能明显抑制小鼠的饮糖行为;②ICR和KM小鼠属于远交系动物,而C57BL/6J小鼠为近交系动物。梁氏情境应激箱处理对3种小鼠的社交行为均具有明显的抑制作用;③梁氏情境应激箱处理对小鼠新奇抑制摄食实验中进食潜伏期和强迫游泳实验中不动时间未见明显影响。结论梁氏情境应激箱处理可产生特定的情境应激反应,引起小鼠典型的抑郁样行为表现。作为一种新的抑郁动物模型及其实验方法,具有良好的表观效度。     

花椒籽油对大鼠动脉粥样硬化的作用研究

目的探究花椒籽油对大鼠脂质代谢的影响,从而探讨其对动脉粥样硬化的作用。方法 24只健康SD大鼠随机分为3组:对照组、高脂组、花椒籽油组,对照组普通饲料喂养,其余两组均高脂饲料喂养,花椒籽油组同时给予花椒籽油灌胃,8周后腹主动脉采血,测定其TC、TG、HDL-C的含量。结果与高脂组相比,花椒籽油组血清TC、TG显著降低(P<0.01,P<0.05),HDL-C显著升高(P<0.01)。结论花椒籽油可有效降低高脂大鼠的TC、TG含量,升高HDL-C含量,对大鼠的动脉粥样硬化形成有一定的防治作用。     

火麻仁油对D-半乳糖致衰老小鼠学习记忆障碍的保护作用研究

目的:研究火麻仁油对D-半乳糖(D-gal)致衰老小鼠学习记忆能力的保护作用,并探讨其作用机制.方法:采用D-半乳糖诱导小鼠衰老模型,用跳台法、水迷宫法和Morris水迷宫法测定衰老小鼠学习记忆能力,检测小鼠脑组织丙二醛(MDA)含量和总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及大脑皮层和海马组织中乙酰胆碱(Ach)含量和乙酰胆碱酯酶(AchE)、胆碱乙酰化转移酶(ChAT)活性,观察脑组织组织形态学变化.结果:火麻仁油(3、6和12 mL/kg)能明显延长跳台实验的跳台潜伏期,缩短错误次数;缩短水迷宫实验达到时间和减少错误次数;缩短Morris水迷宫实验逃避潜伏期和第1次到达原平台时间,增加穿越原平台次数和逗留时间;能显著增加脑组织中T-AOC、SOD、GSH-Px活性和降低MDA含量,增加大脑皮层和海马组织中Ach含量和AchE、ChAT活性,减轻脑组织损伤.结论:火麻仁油可显著改善D-半乳糖致衰老小鼠学习记忆能力,其机制可能与其提高脑组织抗氧化和清除自由基能力,增强中枢胆碱能神经系统功能有关.     

油酰乙醇胺对东莨菪碱诱导小鼠认知功能损伤的保护作用

目的 探讨油酰乙醇胺对东莨菪碱诱导小鼠认知功能损伤的保护作用。方法 将小鼠随机分为6组：对照组、模型组、多奈哌奇组（阳性药,3 mg·kg^-1）和油酰乙醇胺低、中、高剂量（50、100、200 mg·kg^-1）组,每组6只。在ig给药4周后,除对照组外,各组ip 3 mg·kg^-1的东莨菪碱建立阿尔茨海默病（AD）模型。避暗、跳台行为学实验检测小鼠记忆功能; ELISA法检测小鼠海马和大脑皮层中乙酰胆碱（Ach）和乙酰胆碱酯酶（AChE）水平;HE染色观察小鼠大脑皮层及海马损伤。结果 与对照组比较,模型组的避暗潜伏期显著缩短、避暗错误次数显著增加（P<0.001）;大脑皮层、海马的Ach水平显著减少（P<0.01、0.001）,AChE活性显著升高（P<0.001）;模型组的小鼠脑组织形态结构不均匀,组织细胞呈弥散状,提示组织存在病变。与模型组比较,各给药组的避暗潜伏期显著升高、避暗错误次数显著减少（P<0.01）;油酰乙醇胺给药组的小鼠大脑皮层、海马组织Ach水平显著升高（P<0.05、0.01）,AChE活性显著降低（P<0.01、0.001）;小鼠脑组织形态结构病理改变减轻。结论 油酰乙醇胺对东莨菪碱诱导学习记忆障碍模型小鼠的认知功能具有改善作用,其作用机制可能与调节胆碱能系统功能、促进神经细胞存活有关。     

胡萝卜籽油抗胃溃疡作用的研究

目的:观察胡萝卜籽油对实验性胃溃疡的作用.方法:采用十二脂肠注射、灌胃、十二指肠加腹腔注射药方法,分别对幽门结扎型、应激型和利血平型胃溃疡大鼠进行对照实验,通过测量溃疡发生指数,研究胡萝卜籽油对胃溃疡的作用.结果:胡萝卜籽油组与空白对照组相比溃疡发生指数有显著性差异(P＜0.5).结论:胡萝卜籽油对幽门结扎型、应激型和利血平型实验性胃溃疡大鼠均有明显的防治作用.     

超声法提取枸杞多糖工艺研究

目的 优选比较超声法提取枸杞多糖的最佳工艺条件.方法 以枸杞子中枸杞多糖的含量和枸杞多糖的收得率为指标,用比色法测定其含量,用正交试验法L9(34)对实验的提取料液比、提取时间、提取温度和溶液pH值等4个因素进行考察,确定超声法提取枸杞多糖的最佳工艺,并对其结果进行验证.结果 以枸杞多糖的含量和收得率为指标,超声法提取枸杞多糖的最佳工艺是A3B3C2D1.结论 超声提取法提取枸杞多糖具有得率高、操作时间短、有效成分破坏少及提取结果稳定等特点,具有一定的推广意义.     

豆腐果苷对慢性应激小鼠行为学的影响

目的：研究豆腐果苷对慢性应激抑郁小鼠行为学的影响。方法：连续28d给予小鼠慢性不可预见性的刺激与孤养,建立小鼠抑郁模型,同时给予豆腐果苷（40、20、10mg/kg,i.g.）或盐酸氟西汀（2.6mg/kg,i.g.）,采用敞箱试验、蔗糖饮水实验及情绪性行为评分等观察豆腐果苷的抗抑郁行为效应。结果：豆腐果苷可逆转慢性应激小鼠自发活动减少,糖水偏爱度降低,情绪性行为评分及体重的降低。结论：豆腐果苷对慢性应激小鼠具有抗抑郁作用。     

枸杞多糖对小鼠海马神经元细胞系缺糖缺氧损伤保护作用及机制研究

目的探讨枸杞多糖(LBP)对小鼠海马神经元细胞系HT22细胞缺糖缺氧损伤保护作用的机制。方法采用含1 mmol/L连二亚硫酸钠的无糖DMEM培养基建立HT22细胞缺糖缺氧损伤模型,设对照组、模型组和LBP高、中、低剂量(100、50、25 mg/L)组,以CCK8法检测细胞活性,流式细胞术检测检测细胞凋亡情况;Western blotting检测Bcl-2、Bax蛋白表达情况;利用实时荧光定量PCR(RT-qPCR)检测TNF-α、NF-κB、I-κBα、Caspase-9基因转录活性。结果与对照组比较,模型组HT22细胞存活率显著降低、凋亡率显著增加(P<0.05);TNF-α、NF-κB、Caspase-9基因转录活性显著上调,I-κBα基因转录活性显著下调(P<0.05);Bax蛋白表达水平显著升高,Bcl-2的蛋白表达水平显著降低(P<0.05)。与模型组比较,LBP各剂量组细胞存活率显著上升、凋亡率显著下降(P<0.05);TNF-α、NF-κB、Caspase-9基因转录活性显著下调,I-κBα基因转录活性显著上调(P<0.05);Bax蛋白表达水平显著降低,Bcl-2的蛋白表达水平显著增高(P<0.05)。结论 LBP可能是通过下调TNF-α、NF-κB、Caspase-9基因转录活性以及Bax蛋白表达,增强I-κBα基因转录活性及Bcl-2的蛋白表达,抑制缺糖缺氧损伤诱导神经细胞凋亡的发生。     

枸杞子多糖对小鼠白细胞介素-2活性的增强作用(英文)

本文研究了中药枸杞子的提取物枸杞子多糖(LBP)对成年(2月龄)和老年(16月龄)小鼠脾白细胞介素-2(IL-2)活性的影响。在诱导脾细胞IL-2的培养液(脾细胞5×10~6/ml和ConA 4μg/ml)中加入LBP,所制备的含IL-2上清液使成年小鼠胸腺细胞在体外增殖活性([~3H]TdR参入法)升高。老年小鼠IL-2促进淋巴细胞增殖水平显著低于成年。LBP可使老年小鼠IL-2的这种作用显著提高,达成年水平。这些结果表明,LBP对IL-2的活性有增强作用并使老年小鼠IL-2活性得到恢复。     

枸杞多糖抗辐射效应研究

研究表明 ,枸杞中多糖类成分 (LBP)具有较强的生理活性 ,如清除自由基[1 ] ,抗衰老[2 ] ,调节免疫[3,4 ] ,促进精细胞染色体损伤的修复[5] 等 ,但有关LBP的抗辐射作用的研究少见报道。1　材料与方法1 .1　LBP的制备　宁夏枸杞 ,取适量打碎 ,加 1 0倍的水溶液 ,捣浆 ,过滤加乙醇至 60 % ,沉淀物 60℃干燥 ,得LBP。将LBP调配成低剂量 (4g·L- 1 )、中剂量 (1 2g·L- 1 )、高剂量(2 4 g·L- 1 )水溶液备用。1 .2　动物存活率测定　昆明种♂小白鼠 ,体重 (1 7± 2 ) g ,由湖北省卫生防疫站提供。分组灌胃上述 3种剂量LBP ,剂量 0 5ml…     

Moclobemide up-regulates proliferation of hippocarnpal progenitor cells in chronically stressed mice

AIM: To explore the action mechanism of antidepressants. METHODS: The PC12 cell proliferation was detected by flow cytometry,. The proliferation of hippocampal progenitor cells and level of brain-derived neurotrophic factor (BDNF) were measured by immunohistochemistry. RESULTS: Treatment with N-methylaspartate (NMDA) 600μmol/L for 3 d significantly decreased the percentage of S-phase in PC12 cells, while in the presence of classical antidepressant, moclobemide (MOC) 2 and 10μmol/L, the percentage in S-phase increased. Furthermore, the proliferation of progenitor cells in hippocampal dentate gyrus (subgranular zone), as well as the level of BDNF in hippocampus significantly decreased in chronically stressed mice, while chronic administration with MOC 40 mg/kg (ip) up-regulated the progenitor cell proliferation and BDNF level in the same time course. CONLUSION: Up-regulation of the proliferation of hippocampal progenitor cells is one of the action mechanisms for MOC, which may be closely related to the e     

Moclobemide up-regulates proliferation of hippocampal progenitor cells in chronically stressed mice

AIM: To explore the action mechanism of antidepressants. METHODS: The PC 12 cell proliferation was detected by flow cytometry,. The proliferation of hippocampal progenitor cells and level of brain-derived neurotrophic factor (BDNF) were measured by immunohistochemistry. RESULTS: Treatment with N-methylaspartate (NMDA)600 μmol/L for 3 d significantly decreased the percentage of S-phase in PC12 cells, while in the presence of classical antidepressant, moclobemide (MOC) 2 and 10 μnol/L, the percentage in S-phase increased. Furthermore,the proliferation of progenitor cells in hippocampal dentate gyrus (subgranular zone), as well as the level of BDNF in hippocampus significantly decreased in chronically stressed mice, while chronic administration with MOC 40 mg/kg (ip) up-regulated the progenitor cell proliferation and BDNF level in the same time course. CONLUSION:Up-regulation of the proliferation of hippocampal progenitor cells is one of the action mechanisms for MOC, which may be closely related to the elevation of BDNF level at the same time. These results also extend evidence for our hypothesis that up-regulation of the hippocampal neurogenesis is one of the common mechanisms for antidepressants.     

A review of the anticancer and immunomodulatory effects of Lycium barbarum fruit

The anticancer effects of traditional Chinese medicine (TCM) have attracted the attention of the public vis-à-vis existing cancer therapies with various side effects. Lycium barbarum fruit, commonly known as Gou Qi Zi in China, is a potential anticancer agent/adjuvant. Its major active ingredients, L. barbarum polysaccharides (LBP), scopoletin and 2-O-??-d-glucopyranosyl-l-ascorbic acid (AA-2??G), are found to have apoptotic and antiproliferative effects on cancer cell lines. Moreover, LBP also contributes to body??s immunomodulatory effects and enhances effects of other cancer therapies. It is not known whether there are any undesirable effects. Further studies on its pharmacological mechanisms and toxicology could facilitate a safe usage of this TCM herb.     

Warfarin overdose due to the possible effects of Lycium barbarum L.

We reported an 80-year-old Chinese woman on chronic stable dose of warfarin who experienced two episodes of an elevated international normalized ratio (INR) after drinking herbal tea containing Lycium barbarum L. Our case illustrated the potential herbal-drug interaction between warfarin and L. barbarum L. in keeping with a previous case report. Enquiry about herbal intake may be a crucial part in the management of anticoagulation in this locality.     

The antioxidant effects of pumpkin seed oil on subacute aflatoxin poisoning in mice

This study was aimed at the investigation of the antioxidant effect of pumpkin seed oil against the oxidative stress‐inducing potential of aflatoxin. For this purpose, 48 male BALB/c mice were used. Four groups, each comprising 12 mice, were established. Group 1 was maintained as the control group. Group 2 was administered with pumpkin seed oil alone at a dose of 1.5 mL/kg.bw/day (～1375mg/kg.bw/day). Group 3 received aflatoxin (82.45% AFB₁, 10.65% AFB₂, 4.13% AFG_1, and 2.77% AFG₂) alone at a dose of 625 μg/kg.bw/day. Finally, group 4 was given both 1.5 mL/kg.bw/day pumpkin seed oil and 625 μg/kg.bw/day aflatoxin. All administrations were oral, performed with the aid of a gastric tube and continued for a period of 21 days. At the end of day 21, the liver, lungs, kidneys, brain, heart, and spleen of the animals were excised, and the extirpated tissues were homogenized appropriately. Malondialdehyde (MDA) levels and catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH‐Px) activities were determined in tissue homogenates. In conclusion, it was determined that aflatoxin exhibited adverse effects on most of the oxidative stress markers. The administration of pumpkin seed oil diminished aflatoxin‐induced adverse effects. In other words, the values of the group, which was administered with both aflatoxin and pumpkin seed oil, were observed to have drawn closer to the values of the control group. © 2011 Wiley Periodicals, Inc. Environ Toxicol 28: 681–688, 2013.