西安地区学龄前儿童乙肝表面抗体水平调查

目的 探讨学龄前儿童注射乙犁肝炎疫苗后，乙型肝炎表面抗体（HBsAb）的阳性率变化趋势。方法 应用酶联免疫吸附试验（ELISA）法，对4149例儿童入托前进行HBsAb检测。结果 0～2．0岁、2．1～3．0岁、3．1～4．0岁、4．1～5．0岁、5．0—6.0岁儿童乙犁肝炎表面抗体阳性率分别为64．65％、68．64％、71．55％、74．21％、68：38％。绎统计学分析，差异有统计学意义（X^2；19．77，P〈0．05）。结论 新生儿注射乙型肝炎疫苗后，乙型肝炎表面抗体的阳性率随年龄的增高而增高，但到5—6岁后，乙型肝炎表面抗体的阳性率随年龄的增长而里降低趋势。所以6岁以上儿童应进行注射乙型肝炎强化免疫。     

学龄前儿童乙型肝炎表面抗体检测分析

目的了解学龄前儿童乙型肝炎表面抗体状况,为乙型肝炎感染的预防提供资料。方法对159例学龄前儿童采用DR6608时间分辨荧光免疫分析系统检测乙型肝炎表面抗体的结果进行回顾性统计、分析。结果共为159例学龄前儿童检测乙型肝炎表面抗体,表面抗体检测阳性113例(71.07%),其中男童80例,检测结果阳性59例(73.75%);女童79例,检测结果阳性54例(68.35%)。结论应对学龄前儿童进行乙型肝炎表面抗体检测,而为阴性儿童接种乙型肝炎疫苗是防止儿童乙型肝炎感染的有效措施。     

14424人次儿童乙肝表面抗体调查分析

目的了解滨州市儿童对乙型肝炎的免疫力程度,为加强儿童保健提供科学依据。方法对滨州市2005至2007年连续3年的查体结果进行统计整理,按年份、年龄、性别、集体儿童、散居儿童等进行比较。结果滨州市儿童乙肝抗体阳性率保持在65%左右,抗体阳性率：集体儿童71%,散居儿童57%,且随年龄递增。结论集体儿童乙肝免疫力保持在较高水平,散居儿童特别是郊区农村需要加强预防保健。     

学龄前儿童乙肝感染率调查及干预措施

乙型肝炎病毒的传播已成为重要的世界性健康问题。我国是乙肝的流行高发区 ,据报道ＨＢｓＡｇ阳性占总人口的 10 % ,尤其学龄前这一年龄阶段的儿童属预感人群。为防治该病提供科学依据 ,现将我院片区 1999～ 2 0 0 0年入幼儿园健康体检 2～ 6岁儿童 30 5人的ＨＢｓＡｇ及肝功能检测资料分析如下。1　资料与方法1 1　 　资料　我院片区幼儿园入托体检儿童数为 30 5人 ,其中男童 16 1人 ,占 5 2 7% ,女童 14 4人 ,占4 7 1%。1 2　方法　ＨＢｓＡｇ及肝功能检测试剂由上海科华生物技术有限公司和四川迈克科技有限公司提供 ,并按说明操作及判断…     

乙肝疫苗接种后学龄前儿童HBsAg检测分析

笔者从1995年元月～1997年6月对接种过乙肝疫苗的学龄前儿童进行HB_sAg检测,发现有部分儿童HB_sAg阳性,现将结果报道如下。 1 材料与方法 1.1 检测对象 1995年元月～1997年6月来我院进行体检学龄前儿童共5435人(均已接种过乙肝疫苗)。 1.2 试剂 由上海科华实业有限公司提供,按说明书操作。 1.3 方法 手指微量血筛查,可疑者抽静脉血复查,均采用ELISA方法。其结果见表1。     

乙肝表面抗体阳性模式分析

随着基础免疫的加强，乙肝表面抗体(HBsAb)阳性的血清模式变化呈现多样化。笔者对我院2001-2003年三年来的HBsAb的变化模式进行了分析。     

育龄妇女乙肝表面抗体检测250例临床分析

目的了解育龄妇女乙肝表面抗体状况,为阻断乙肝母婴传播提供资料。方法对250例育龄妇女采用时间分辨荧光免疫法检测乙肝表面抗体结果并进行统计分析。结果育龄妇女乙肝表面抗体检测250例,检测阴性88例,阴性率35．2％,检测阳性162例,阳性率为64．8％。结论育龄妇女乙肝表面抗体阴性仍有较高的比例,孕前检测乙肝表面抗体并对阴性者及时补种乙肝疫苗,可有效预防母亲乙肝感染及阻断乙肝母婴传播。     

内江市乙肝表面抗体阳性结果分析

对2000年12月-2001年12月来我院检测乙肝两对半的4918例的抗-HBs进行统计,以了解我市现阶段抗-HBs阳性状况,为综合防治提供参考。1 材料和方法1.1 检测对象:2000年12月-2001年12月来我院体检的离     

青岛市婚育人群及学龄前儿童HBsAg阳性情况调查

对7582名婚检查、2538名产前建卡孕妇、2665名学龄前入托儿童进行了HBsAg检测,结果;婚检者在三组人群中HBsAg携带率最高,为5.57％,其中男性、女性HBsAg阳性率分另为6.60％、4.69％,两者有极显著差异(X~2=12.82,P<0.01)。提示青年男子HBV感染情况不容乐观。孕妇与儿童HBsAg阳性率分别为4.65％、2.44％,两者有极显著差异(X~2=18.72,P<0.01)。婚检人群与孕妇之间无显著差异(X~2=3.17,P>0.05)。     

乙肝血清标志物与HBV-DNA定量检测结果分析

目的观察分析乙肝血清标志物与HBV-DNA定量检测的结果,讨论其相关性。方法选取乙型肝炎患者340例为观察组,60例健康体检者为对照组,分析其一般资料,对其进行乙肝血清标志物检验和HBV-DNA定量检测,分析两种检测方法的结果及稳定性、重复性。结果对照组两检测全阴性,重复性及稳定性良好,HBV-M检测结果不同HBV-DNA阳性构成比差异有显著统计学意义(P<0.01),大三阳HBV-DNA的构成比较高,HBV-DNA定量值较高且随病程延长下降,1.2%HBV-DNA阳性患者HBV-M检测全阴性。结论采用ELISA检测乙肝血清标志物容易导致对慢性病毒性乙肝漏诊,对其传染性及治疗效果分析不足,可采用PCR检测HBV-DNA或联合检测,有助于提高对患者病情发展的检测准确性。     

血清乙肝病毒核心抗体定量检测在慢性乙肝患者病情评价中的应用价值

目的 探讨血清乙肝病毒核心抗体定量检测在慢性乙肝患者病情评价中的应用价值。方法 选取2019年3月～2021年5月某院门诊部和住院部收治的128例慢性乙肝病毒感染患者为研究对象,上述患者分为慢性乙型肝炎组（CHB组,n=72）和肝硬化组（LC组,n=56）。比较两组血清HBc Ab定量滴度与HBV血清标志物、血清生化指标和肝脏纤维化程度的关系。结果 LC组HBs Ag的定量滴度显著低于CHB组（P <0.05）,HBc Ab和HBV-DNA的定量滴度显著高于CHB组（P <0.05）。LC组的ALT、AST、ALB值显著低于CHB组（P <0.05）,TBil值明显高于CHB组。LC组HBc Ab滴度水平与HBV-DNA无显著相关性（r=-0.010, P> 0.05）,与ALT呈显著正相关（r=0.316, P <0.05）。LC组的HBs Ag和HBc Ab的滴度水平与未出现肝纤维化组、轻度纤维化组、中度纤维化组和重度纤维化组的差异具有统计学意义（P <0.05）。HBV感染患者的肝纤维化发展程度与其HBs Ag滴度水平呈负相关（r=-0.697...     

127例乙肝表面抗体ELISA检测与化学发光法的比较

目的 探讨浓度处于5～100mIU/ml的乙肝表面抗体(HBsAb)ELISA定性检测与化学发光法的符合情况.方法 采用10种国产主流HBsAb ELISA试剂对127例经用化学发光法检测已知HBsAb浓度的标本进行检测,分析ELISA检测OD值与抗体浓度的相关性,并以试剂盒临界值为准分别比较不同OD值段ELISA结果 与化学发光法结果 的符合率.结果 10种同产HBsAb ELISA试剂盒OD值与化学发光定最值均呈正相关(P<0.01);ELISA法检测结果 与化学发光的阴阳符合率比较显示,5～10mIU/ml样本符合率较低,只有37.5%,在大于100mIU/ml时可达99.4%,在10～30mIU/ml,30～100mIU/ml分别为75.3%和90.7%;OD值存0～0.05,0.05～0.105、0.105～0.21、0.21～0.3、0.3～0.5、大于0.5分别为73.4%、65.3%、60.5%、72.0%、86.5%、92.2%.结果 显示阴阳结果 符合率在较高在大于0.5时最高,在介于0.105～0.21的符合率最低;处于试剂盒临界值±20%处的标本的符合率普遍偏低,最低只有42%,最高亦不超过80%.结论 虽然10种国产ELISA试剂之间的检测结果 没有统计学差异,但是对于浓度介于5～10mIU/ml标本符合率低.而且在接近试剂盒临界值时与化学发光结果 符合率则很低,但是随着OD值的增高符合率也逐渐升高.考虑到乙肝表面抗体的特殊临床意义,如何选取一个更为科学客观的结果 判断临界值值得进一步探讨.     

乙肝表面抗原和表面抗体同时阳性对乙肝性肝癌的预测价值

目的 研究乙型病毒性肝炎(乙肝)表面抗原(HBsAg)和乙肝表面抗体(抗-HBs)同时阳性对乙肝性肝癌的预测价值。方法 回顾性分析2016～2018年在永泰县医院就诊的乙肝患者,通过电化学发光免疫分析法(ECLIA)检测患者的乙型肝炎病毒(HBV)血清标志物,共发现HBsAg3569份阳性样本,其中HBsAg和抗-HBs同时阳性的患者261例,仅HBsAg阳性患者3308例,经过4～5年的病情演变, HBsAg和抗-HBs同时阳性的患者中有25例发展为肝癌(病例组),仅HBsAg阳性患者中有165例发展为肝癌(对照组)。比较HBsAg阳性与HBsAg和抗-HBs同时阳性患者的相关指标[丙氨酸氨基转移酶(ALT)、总胆红素(TBIL)、HBsAg、HBV-DNA、肝癌发生率]及肝癌与非肝癌患者的临床资料。结果 HBsAg阳性与HBsAg和抗-HBs同时阳性患者TBIL、ALT比较,差异无统计学意义(P>0.05);HBsAg和抗-HBs同时阳性患者HBsAg(2845.7±2822.6)COI、HBV-DNA(2.62±1.85)IU/ml低于HBsAg阳性患者的(5851.5±1...     

127例乙肝表面抗体ELISA检测与化学发光法的比较

Objective To compare the difference between ELISA and chemiluminescence assay in de-tecting hepatitis B surface antibody (HBsAb) serum valued in 5～100mIU/ml. Method 127 HBsAb serum samples that had valued by chemiluminescence were detected using 10 domestic ELISA kits. The correlation between the results of ELISA kits and chemiluminescence assay were analyzed. Coincidence between the results of ELISA kits and chemiluminescence at different OD value range were also analyzed. Results The detected results between 10 domestic ELISA kits and chemiluminescence assay are positive correlation (P<0.01); The optimal coincidence rates of the results of ELISA with those samples valued by chemiluminescence at more than 100mIU/ml was 99.4%; the coinci-dence rate is from 42% to 80% in cut-off±20%. The average coincidence rate is under 60%. Conclusions Though the detected results of 10 domestic ELISA reagents is related closely, the coincidence rate of the results at 5～10mIU/ ml in detecting anti-HBs is low. So it is important to make a positive standard in detecting anti-HBs.     

127例乙肝表面抗体ELISA检测与化学发光法的比较

Objective To compare the difference between ELISA and chemiluminescence assay in de-tecting hepatitis B surface antibody (HBsAb) serum valued in 5～100mIU/ml. Method 127 HBsAb serum samples that had valued by chemiluminescence were detected using 10 domestic ELISA kits. The correlation between the results of ELISA kits and chemiluminescence assay were analyzed. Coincidence between the results of ELISA kits and chemiluminescence at different OD value range were also analyzed. Results The detected results between 10 domestic ELISA kits and chemiluminescence assay are positive correlation (P<0.01); The optimal coincidence rates of the results of ELISA with those samples valued by chemiluminescence at more than 100mIU/ml was 99.4%; the coinci-dence rate is from 42% to 80% in cut-off±20%. The average coincidence rate is under 60%. Conclusions Though the detected results of 10 domestic ELISA reagents is related closely, the coincidence rate of the results at 5～10mIU/ ml in detecting anti-HBs is low. So it is important to make a positive standard in detecting anti-HBs.     

127例乙肝表面抗体ELISA检测与化学发光法的比较

Objective To compare the difference between ELISA and chemiluminescence assay in de-tecting hepatitis B surface antibody (HBsAb) serum valued in 5～100mIU/ml. Method 127 HBsAb serum samples that had valued by chemiluminescence were detected using 10 domestic ELISA kits. The correlation between the results of ELISA kits and chemiluminescence assay were analyzed. Coincidence between the results of ELISA kits and chemiluminescence at different OD value range were also analyzed. Results The detected results between 10 domestic ELISA kits and chemiluminescence assay are positive correlation (P<0.01); The optimal coincidence rates of the results of ELISA with those samples valued by chemiluminescence at more than 100mIU/ml was 99.4%; the coinci-dence rate is from 42% to 80% in cut-off±20%. The average coincidence rate is under 60%. Conclusions Though the detected results of 10 domestic ELISA reagents is related closely, the coincidence rate of the results at 5～10mIU/ ml in detecting anti-HBs is low. So it is important to make a positive standard in detecting anti-HBs.     

127例乙肝表面抗体ELISA检测与化学发光法的比较

Objective To compare the difference between ELISA and chemiluminescence assay in de-tecting hepatitis B surface antibody (HBsAb) serum valued in 5～100mIU/ml. Method 127 HBsAb serum samples that had valued by chemiluminescence were detected using 10 domestic ELISA kits. The correlation between the results of ELISA kits and chemiluminescence assay were analyzed. Coincidence between the results of ELISA kits and chemiluminescence at different OD value range were also analyzed. Results The detected results between 10 domestic ELISA kits and chemiluminescence assay are positive correlation (P<0.01); The optimal coincidence rates of the results of ELISA with those samples valued by chemiluminescence at more than 100mIU/ml was 99.4%; the coinci-dence rate is from 42% to 80% in cut-off±20%. The average coincidence rate is under 60%. Conclusions Though the detected results of 10 domestic ELISA reagents is related closely, the coincidence rate of the results at 5～10mIU/ ml in detecting anti-HBs is low. So it is important to make a positive standard in detecting anti-HBs.     

127例乙肝表面抗体ELISA检测与化学发光法的比较

Objective To compare the difference between ELISA and chemiluminescence assay in de-tecting hepatitis B surface antibody (HBsAb) serum valued in 5～100mIU/ml. Method 127 HBsAb serum samples that had valued by chemiluminescence were detected using 10 domestic ELISA kits. The correlation between the results of ELISA kits and chemiluminescence assay were analyzed. Coincidence between the results of ELISA kits and chemiluminescence at different OD value range were also analyzed. Results The detected results between 10 domestic ELISA kits and chemiluminescence assay are positive correlation (P<0.01); The optimal coincidence rates of the results of ELISA with those samples valued by chemiluminescence at more than 100mIU/ml was 99.4%; the coinci-dence rate is from 42% to 80% in cut-off±20%. The average coincidence rate is under 60%. Conclusions Though the detected results of 10 domestic ELISA reagents is related closely, the coincidence rate of the results at 5～10mIU/ ml in detecting anti-HBs is low. So it is important to make a positive standard in detecting anti-HBs.     

127例乙肝表面抗体ELISA检测与化学发光法的比较

Objective To compare the difference between ELISA and chemiluminescence assay in de-tecting hepatitis B surface antibody (HBsAb) serum valued in 5～100mIU/ml. Method 127 HBsAb serum samples that had valued by chemiluminescence were detected using 10 domestic ELISA kits. The correlation between the results of ELISA kits and chemiluminescence assay were analyzed. Coincidence between the results of ELISA kits and chemiluminescence at different OD value range were also analyzed. Results The detected results between 10 domestic ELISA kits and chemiluminescence assay are positive correlation (P<0.01); The optimal coincidence rates of the results of ELISA with those samples valued by chemiluminescence at more than 100mIU/ml was 99.4%; the coinci-dence rate is from 42% to 80% in cut-off±20%. The average coincidence rate is under 60%. Conclusions Though the detected results of 10 domestic ELISA reagents is related closely, the coincidence rate of the results at 5～10mIU/ ml in detecting anti-HBs is low. So it is important to make a positive standard in detecting anti-HBs.     

127例乙肝表面抗体ELISA检测与化学发光法的比较

Objective To compare the difference between ELISA and chemiluminescence assay in de-tecting hepatitis B surface antibody (HBsAb) serum valued in 5～100mIU/ml. Method 127 HBsAb serum samples that had valued by chemiluminescence were detected using 10 domestic ELISA kits. The correlation between the results of ELISA kits and chemiluminescence assay were analyzed. Coincidence between the results of ELISA kits and chemiluminescence at different OD value range were also analyzed. Results The detected results between 10 domestic ELISA kits and chemiluminescence assay are positive correlation (P<0.01); The optimal coincidence rates of the results of ELISA with those samples valued by chemiluminescence at more than 100mIU/ml was 99.4%; the coinci-dence rate is from 42% to 80% in cut-off±20%. The average coincidence rate is under 60%. Conclusions Though the detected results of 10 domestic ELISA reagents is related closely, the coincidence rate of the results at 5～10mIU/ ml in detecting anti-HBs is low. So it is important to make a positive standard in detecting anti-HBs.