两性霉素B脂质体对小鼠白色念菌感染的治疗观察

两性霉素Ｂ脂质体与未包封脂质体的两性霉素的小鼠急毒试验ＬＤ５０分别为５．０７ｍｇ．ｋｇ＾－１和２．６１ｍｇ．ｋｇ＾－１。，对全身性白色念珠菌感染的实验治疗，再会两者等剂量，小鼠存活率分别为５５％和３５％，判别不明显。     

鹿茸多肽对实验性骨折的治疗作用及机理研究

目的：探讨鹿茸多肽（ＰＡＰ）治疗骨折的作用机理,方法：离体观察鹿茸多肽对＾３Ｈ－ＴｄＲ掺入家兔肋软骨,人胚关节软骨及鸡胚头盖骨成骨样细胞的影响。整体利用大鼠完全缺损性桡骨骨折模型,观察鹿茸多肽１０和２０ｍｇ·ｋｇ＾－１剂量对骨痂形成,骨折的愈合以及骨痂内羟脯氨酸和钙含量的影响。结果：离体试验表明,鹿茸多肽１０～５０ｍｇ·Ｌ＾－１对家兔和人胚软骨细胞以及鸡胚头盖骨成骨样细胞都有促进有丝分裂作用,提示     

静脉丙种球蛋白对病毒性心肌炎作用的研究

为探讨国产人静脉血丙种球蛋对病毒性心肌炎的保护及治疗作用,取ＢＡＬＢ／ｃ小鼠随机分为ＩＶＩＧ保护组与治疗组。保护组接种柯萨奇Ｂ３病毒后,立即使用剂分别为２００ｍｇ．ｋｇ＾－１．ｄ＾－１,５００ｍｇ．ｋｇ＾－１．ｄ＾－１、１ｇ．ｋｇ＾－１．ｄ＾－１的ＩＶＩＧ７天。     

马钱子碱的药代动力学

用荧光分光光度法测定马钱子碱在小鼠体内的血药肖度及其药代动力学参数。结果表明：小鼠尾静脉注射３种剂量１０，７．５和５ｍｇ．ｋｇ＾－１的Ｂ后时量曲线均符合二室开放模型。其主要动力学参数：Ｔ＾１／２ａ分别为０．１７，０．２２和０．１ｈ；Ｔ１／２β分别为１．９６，２．１１和２．０６ｈ；Ｖｃ分别为７．２３，７．５９和５６．６５Ｌ．ｋｇ＾－１ＡＵＣ分别为２．４８，１．９６和１．４５ｈ．ｍｇ。；＾－１；ＣＬｓ     

红黄通脉冲剂活血化瘀作用实验研究

红黄通脉冲剂（ＨＨＴＭ）灌胃给药，１００ｍｇ．（ｋｇ．ｄ＾－１）和２００ｍｇ．（ｋｇ．ｄ）＾－１以其所含总黄酮量计算），连续了７天。结果：均可明显延长小鼠的凝血时间和凝血酶原时间，抑制家兔的血栓形成和血小板的聚集率。     

富硒康毒性的实验研究

小鼠最大耐受量的富硒康６４００ｍｇ．ｋｇ＾－１（为临床推荐用量的８００倍）口服，观察２周，未见死亡和任何中毒症状。简化机率单位法求出腹腔注射富硒康的ＬＤ５０为１３０．１４ｍｇ．ｋｇ＾－１（１２２．０６－１３８．７５ｍｇ．ｋｇ＾－１）。长期毒性实验给大鼠（１００、２００、４００ｍｇ．ｋｇ＾－１ｄ＾－１和狗（２４、２４０ｍｇ．ｋｇ＾－１．ｄ＾－１）连续口服富硒康４５天（相当于临床推荐用量的３－５０倍）     

国产单硝酸异山梨醇酯注射剂对家兔多种离体血管平滑肌的影响

目的：研究国产单硝酸异山梨醇酯注射剂对多种离体血管平滑肌的影响。方法：观察该药物对去甲肾上腺素及氯化钾分别引起家兔多种离体血管平滑肌收缩活动的影响。结果：单硝酸异山梨醇酯对２×１０＾－６ｍｏｌ．Ｌ＾－１ＮＥ引起兔离和主动脉、肺动脉、肾动脉、股动脉螺旋条有静脉、股静脉、门静脉血管条收缩呈浓度依赖性抑制作用,其半数抑制浓度依次为（５．５±０．６、２．６±０．１、３．０±０．１、８．７±０．５、０．４±     

脂糖舒对大鼠离体小肠运动的影响

观察纯中药复方脂糖舒对小肠运动的影响。方法：大鼠随机分组,采用离休肠平滑肌实验装置,应用三种不同浓度脂糖舒（０．２０％、０．６０％、１．２０％）,观察其对离体十二指肠和回肠自发运动的作用及高浓度脂糖舒（１．２０％）对乙酰胆碱、氯化钡、肾上腺素的拮抗作用。结果：各浓度脂糖舒对十二指肠自发运动均有抑制作用,且随剂量增大而增强;高浓度脂糖舒对乙酰胆碱、氯化钡、紧不素有拮抗作用,对乙酰胆碱的拮抗作用最明显     

党参和丹参对兔离体主动脉平滑肌运动的影响

目的 观察党参和丹参水煎对兔胸主动脉血管平滑肌条舒缩活动的影响。方法 采用离体血管张力实验法,观察党参和丹参对ＮＥ和ＫＣＬ所致肌条收缩活动的影响及与内此细胞之间的关系。结果 （１）４０ｍｇ／ｍｌ党参对ＮＥ和ＫＣＩ所致免胸主动脉血管平滑肌条肌收缩量效曲线无明显影响,俣可抑制１０＾－６ｍｏｌ／Ｌ ＮＥ 引起的内皮完整的主动脉肌条的预收缩作用（Ｐ〈０．００１）,去内皮或加入Ｌ－ＮＮＡ后其舒张作用明显减弱     

合用赖氨匹林和呋喃苯胺酸的平喘作用

探讨合用赖氨匹林和呋喃苯胺酸的平喘作用，方法 采用整体动物引喘法和兔改良肺溢流法观察合用氨匹林和呋喃苯妥酸的平喘作用和对兔支气管平滑肌张力的影响。结果 赖氨匹林１５０ｍｇ．ｋｇ＾－１＋呋喃苯妥酸２０ｍｇ．ｋｇ＾－１，赖氨匹林３００ｍｇ．ｋｇ＾－１＋呋喃苯胺酸２０ｍｇ．ｋｇ＾－１均可明显延长豚鼠的引喘潜伏期及减少兔肺溢流量。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.