THE AUTOCRINE REGULATORY EFFECT OF VASOACTIVE INTESTINAL PEPTIDE ON THE GROWTH OF HUMAN PANCREATIC CARCINOMA CELLS

In the present study, the effets of VIP on the growth of two human pancreatic carcinoma cell lines PU-PAH-1 and PANC-1 were determined using tritiated thymidine incorporation, VIP receptors, intracellular cAMP and polyamings were investlsa.ted, The results indicated that VIP at a concentcation of 10^-8mol/L to 10^-7 mol/L can significantly Stimulate the growth of PU-PAN-1 cells but not PANC-1 cells, This effect is dose-dependent and abolished by VIP receptor antagonist, [4-CI-Phe^6 , Leu^7] VIP, suggesting VIP receptors in PU-PAN-1 cells maymediate this effect. VIP can markedly elevate the levels of intracellular cAMP and polyammes in PU-PAN-1 cells,indicating that the growth-promoting effect stimulated by VIP may be via a rapid increase in the biosynth~es of cAMP and polyamines. In addition, the VIP-antibody ir2Libited the growth of PU-PAN-1 cells in serum-free culture medium. The results above suggested that VIP has an autoctine regulatory effect on this pancreatic carcinoma cell line(PU-PAN-1).     

EFFECT OF TAMOXIFEN ON THE SECRETORY FUNCTION OF HUMAN TROPHOBLASTIC CELLS

Gu et al observed the morphological damages and the suppressed secretion of HCG in vitro cultured human placental chorionic tissues caused by tamoxifen (20ug/ml).TarnoxiFeu blood concentrations, during oral administration. 20rag, b.i.d, in early pregnant     

EXPERIMENTAL STUDY ON DIMETHYLSULFOXIDEINDUCED DIFFERENTIATION OF HUMAN ESOPHAGEALEPITHELIAL CARCINOMA CELLS

ResumeObjeCtif11adidddmontrdqueietraitementdestumeurssolidespari'inductiondeladill6renciationn'etaitpasaussiefficacequeceluideleucdmieaiguepromydlocytaire(LAP)parl'acideretinoiquetouttrans,Antifestdonen4cessaired'eoplorerd'autresinducteurspourlathdrapiedestumeurssolide.dimethylsulloxyde(DMSO)estactuellementconsiderscommecandidat.WthodesEnutilisantDMSOcommeinducteurdansuneligndecancdreusedpithelialed'OesOPhagehamaineetcomparantavecARtt,nonsavonsetudidlamoPPhologtecellulaire,iecyclecellu…     

THE EFFECT OF ACUPUNCTURE ON THE PHAGOCYTIC ACTIVITY OF HUMAN LEUKOCYTES

To study the effect of acupuncture in treatmentof infectious diseases, 66 adult patients in the wardsof general surgery were given acupuncture once dailyfor 3 days after operation.Of 40 patients with ma-lignant tumors, 20 received acupuncture and 20 serv-ed as controls.Of 26 patients with inflammatorylesions,17 received acupuncture, and 9 served ascontrol.Acupoints used were Zusanli(St 36)sup-plemented by Neiguan(P 6)or Sanyin1jiao(Sp 6)ac-cording to the type of operation.It was shown that,     

EFFECT OF ACUPUNCTURE ON VASOACTIVE INTESTINAL PEPTIDE IN ISCHEMIC CEREBRO VASCULAR DISEASES

Background and purpose: Vasoactive intestinal peptide (VIP) appears to play an important role as a neurotransmitter or neuromediater in ischemic cerebrovascular diseases (ICVD). The effect of acupuncture, which is used in treatment of ICVD with good efficiency, on VIP has not been known. For finding the mechanism of acupuncture in treatment of ICVD and the effect of electro-acupuncture on VIP, the present study was performed. Materials and methods: 59 patients with acute ICVD were randomly divided into two groups. Electro-acupuncture and routine treatment were given in Group 1 (n= 29), and routine treatment was used alone in Group 2 (n= 30). The cerebrospinal fluid (CSF) and blood were taken before the beginning of treatment and after a course of treatment in both groups. The control group consisted of 38 cases of non-ICVD. VIP was measured by radioimmunoassay. Results: The level of CSF VIP in patients with acute ICVD was significantly lower than that in the controls, while the levels of plamsa VIP showed no significant difference between the ICVD and control groups, and the level of CSF VIP was not significantly correlated with the level of plasma VIP. After acupuncture treatment, the level of CSF VIP was increased and showed no significant difference as compared with the control group. Conclusion: Acupuncture might alleviate the disturbance of metabolism of VIP in CNS.     

THE EFFECT OF RADIX SALVIAE MILTIORRHIZAE ON VASOACTIVE INTESTINAL PEPTIDE IN CEREBRAL ISCHEMIA:AN ANIMAL EXPERIMENT

The levels of vasoactive intestinal peptide(VIP)in three regions of rat brain were assayed in 62 rats.Bilateral common carotid artery ligation was donein 50 rats.Half an hour before ligation 26 rats weregiven 10 g/kg of Radix Salviae Miltiorrhizae(RSM);24 rats were given same volume of normalsaline as controls.A sham operation wasdone in 12 rats.Half an hour(n=30)and 3hours(n=32)after operation,the rats were quicklydecapitated.VIP levels were assayed in cerebralcortex,hippocampus and caudate nucleus,in salin-treated animals,VIP levels of cerebral cortex andcaudate nucleus at 3 hour group were significantlydecreased compared with the sham-operated group.No significant difference was found between RSM-treated and sham-operated groups.The preliminaryresults suggest that VIP may be involved in thepathophysiological procedures of cerebral ischemiaand RSM may attenuate the dysfunction of VIPduring cerebral ischemia.     

EFFECT OF MULTIGLYCOSIDES OF TRIPTERYGIUM WILFORDH ON THE FUNCTION OF LEYDIG CELLS

Animal experiments and clinical researclies showed that multiglycosides of Tripterygium wilfordii (GTW) had male contraceptive effects, At contraceptive doses, GTW did not significantly affect the plasma level of testosterone, but higher doses of GTW did decrease     

OBSERVATION OF THE EFFECT OF SPIROBOLUS BUNGII EXTRACT ON CANCER CELLS

Mice with ascites type (U_(14)A) carcinomawere treated (i.p.200 mg/kg once only) withextract of Spirobolus bungii Brandt.An increasein cancer cell death ratio of 35% was seen 2hours after treatment.The injured cells pre-sented karyopyknosis,karyoclasis,cytoclasis andvacuolization.Tumor mitosis was immediatelyarrested.Even 24 hours after exposure the ratioof mitosis was below the starting level.Scan-ning electron microscopy also revealed signifi-cant changes:the cancer cell plasma membraneappeared crenate in the form of villus-like pro-jections and the surface of the membrane be-     

RELAXANT EFFECTS OF VASOACTIVE INTESTINAL PEPTIDE ON PULMONARY ARTERY IN CHRONICALLY HYPOXIC RATS

ＲＥＬＡＸＡＮＴＥＦＦＥＣＴＳＯＦＶＡＳＯＡＣＴＩＶＥＩＮＴＥＳＴＩＮＡＬＰＥＰＴＩＤＥＯＮＰＵＬＭＯＮＡＲＹＡＲＴＥＲＹＩＮＣＨＲＯＮＩＣＡＬＬＹＨＹＰＯＸＩＣＲＡＴＳＣｈｅｎＹｕｌｉｎｇ陈玉玲ａｎｄＬｕｏＷｅｉｃｉ罗慰慈（ＰｅｋｉｎｇＵｎｉｏｎＭ...     

乙双吗啉和γ射线联合应用对小鼠小肠上皮细胞的影响

本文研究乙双吗啉(AT1727)和γ射线联合应用对动物小肠上皮细胞的作用。腹腔注射AT1727 160mg/kg,在放射前30min给药时,其斜率和单纯放射组相仿,DMF值为1.09;放射后30min给药时,D_o值增加1.3倍,DMF值为1.05。在使用相等分割剂量做分割放射时,显示出他们之间明显的相互作用。结果显示:联合应用增加了辐射生物效应,且和所用药物浓度有关,和二者之间应用次序和相隔时间也有关。     

人脑海马内部微血管三维构筑的研究

本文收集广州地区６个月胎儿至６岁儿童新鲜大脑标本６３例（１２６侧），其中５例作脑血管有机玻璃单体铸型，３５例用碱性磷酸酶染色（包括７例墨汁灌注后复染），其余用于墨汁灌注法，在光镜暗视野全景放大观察和日立Ｓ－４５０扫描电镜观察，较完整地显示人脑海马内部血管动脉－微动脉－毛细血管前括约肌－毛细血管－微静脉－小静脉连续性立体构筑。此外还观察到微血管形态特征及微血管间存在多种吻合。     

血管活性肠肽对大鼠离体逼尿肌肌条自发性收缩作用的实验研究

目的：观察血管活性肠肽（vasoactive intestinal peptide,VIP)对离体逼尿肌肌条自发性收缩频率及收缩幅度的作用。方法：建立Wistar大鼠下尿路梗阻动物模型,6周后行充盈性膀胱测压,根据逼尿肌稳定与否将下尿路梗阻动物分为逼尿肌稳定组和逼尿肌不稳定组,用拉力传感器观察VIP对离体逼尿肌肌条的自发性收缩频率及收缩幅度的影响。结果：大鼠下尿路梗阻6周后膀胱逼尿肌不稳定发生率为62．1％,VIP能明显抑制逼尿肌的自发性收缩,降低逼尿肌肌条自发性收缩频率及收缩幅度（P＜0．05）,但梗阻后逼尿肌不稳定组,逼尿肌稳定组及对照组之间差异无显著性意义（P＞0．05）,结论：VIP能显著抑制正常和梗阻后逼尿肌自发性收缩,但其在逼尿肌不稳定发生中的作用仍需深入研究。     

肝硬化和消化性溃疡患者血浆血管活性肠肽水平的变化

为了探讨肝硬化和消化性溃疡患者血管活性肠肽的变化及其调节机制。方法测定了２０年例肝硬化和２０例消化性溃疡患者的血浆ＶＩＰ水平。     

胰腺癌肿瘤性微血管的定量检测

①目的探讨胰腺癌组织肿瘤性微血管（ＮＭＶ）定量与其病理形态及生物学行为的关系。②方法采用免疫组化法检测第Ⅷ因子相关抗原的表达，对４７例胰腺癌组织的肿瘤性微血管进行定量测定。③结果低分化腺癌微血管数量明显高于高分化腺癌，组织学分级Ⅲ级和临床晚期（Ⅲ，Ⅳ期）病人高于Ⅰ级和临床早期（Ⅰ，Ⅱ期）病人，差别均有极显著性（ｔ＝４．８７３，４．５３９，５．２５７，Ｐ＜０．００１）。④结论胰腺癌组织肿瘤性微血管生成与其组织学特征及生物学行为密切相关。     

单克隆抗体对活化腹腔巨噬细胞抗胰腺癌作用的影响

观察重组人白细胞介素２（γ－ＩＬ２）体外诱导的腹腔巨噬细胞，在ＹＰＣ３抗人胰腺癌单克隆抗体（ＹＰＣ３ｍＡｂ）介导下，通过抗体依赖性细胞介导的细胞毒性（ＡＤＣＣ）机制对胰腺癌细胞的杀伤作用。４ｈ５１Ｃｒ释放实验表明，在ＹＰＣ３ｍＡｂ作用下，活化腹腔巨噬细胞对Ｃａｐａｎ－２人胰腺癌细胞的杀伤活力明显增强，比单用巨噬细胞杀伤活力约提高７０．０％，而对照１－Ｆ／７抗登革热病毒单抗无明显影响。γ－ＩＬ２作用的腹腔巨噬细胞和脾淋巴细胞具有类似的ＡＤＣＣ杀伤效应。结果提示，γ－ＩＬ２和ＹＰＣ３ｍＡｂ联合腹腔注射，可能为胰腺癌的局部治疗提供一个新方法。     

电针大鼠“足三里”对交叉上核VIP免疫反应的影响

以免疫细胞化学与图像处理结果分析发现，针刺大鼠“足三里”不仅义叉上核中的血管活性肠肽免疫反应阳性神经元胞体数目显著增加，而且血管活性肠肽免疫反应性也显著增加。提示电针“足三里”所致的交叉上核血管活性肠肽免疫反应性的改变可能参与调节机体神经内分泌活动的昼夜节律。     

大鼠肠神经系统内γ-氨基丁酸能神经元与乙酰胆碱、一氧化氮、血管活性肠肽的共存关系

目的:探讨-氨基丁酸(GABA)在大鼠肠神经系统中与乙酰胆碱、一氧化氮、血管活性肠肽的共存关系。方法:应用免疫组织化学和免疫荧光双重染色技术对大鼠肠神经系统内谷氨酸脱羧酶(GAD)与乙酰胆碱酯酶(AchE)、一氧化氮合酶(NOS)、血管活性肠肽(VIP)的共存关系进行观察。结果:GAD样阳性神经元主要位于肌间神经丛,与AchE、NOS、VIP存在共存关系。双重标记神经元分别占GAD阳性神经元的5.6%、62.6%和51.1%,而占AchE、NOS、VIP阳性神经元的3.4%、19.8%和43.5%。结论:在肠神经系统中GABA可能通过调节其它神经递质的释放而发挥着间接的调控作用。     

雌激素受体与大肠癌的关系

应用免疫组织化学技术对４８例大肠癌标本进行了雌激素受体（ＥＲ）检测。结果发现ＥＲ的阳性率为３７．５％。４８例大肠癌按组织学分型，ＥＲ阳性率分别为：高分化腺癌组５２．３％；中分化腺癌组３８．９％；低分化腺癌组１１．１％。高分化腺癌与低分化腺癌组比较，ＥＲ阳性率有显著差别（ｐ〈０．０１）。４８例大肠癌标本按部位、性别、年龄分组，１９例结肠癌中，ＥＲ阳性率４２．１％，２９例直肠癌中，ＥＲ阳性率３４．５％