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胃康复对脾虚证胃粘膜癌前病变上皮细胞核及线粒?… 总被引:7,自引:0,他引:7
目的:观察胃康复冲剂对胃粘膜上皮细胞核、线粒体Zn、Cu和DNA含量的影响。方法:应用胃康复冲剂治疗61例脾虚证胃粘膜肠上皮化生(IM)和不典型增生(ATP)患者,治疗前后均作胃镜检查,取胃窦部粘膜测定细胞核与线粒体的微量元素及细胞核DNA含量。结果:4组脾虚证和4组IM亚型,在治疗前胃粘膜上皮细胞核Zn、Cu和DNA水平均较健康对照组高,而线粒体Zn、Cu水平则较健康对照组低(P〈0.05~0. 相似文献
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脾虚证胃病胃粘膜亚细胞结构及其微量元素、DNA研究的临床意义 总被引:4,自引:0,他引:4
采用透射电镜、能谱仪、图象分析技术和组织化学染色,对88例脾虚证胃病患者的胃粘 膜,进行肠化生分型、亚细胞结构、Zn、Cu和DNA检测。发现细胞核与核仁增大、分叶枝增多、染色 质密集、线粒体退变,以及核内Zn,Cu和DNA含量,随下列顺序递增:健康对照、脾气虚、脾虚气 滞证,慢性浅表性胃炎→慢性萎缩性胃炎→胃癌;肠化生完全性小肠型→不完全性小肠型→完全性 结肠型→不完全性结肠型。线粒体及其峙的数量,线粒体内Zn、Cu含量,则随以上顺序递减。提示 脾虚气滞证胃病以细胞核蛋白合成代谢旺盛、线粒体能量代谢衰微为特征,也就是以细胞分裂增殖 加速、细胞分化障碍为病理特征;脾气虚以细胞核、线粒体二者代谢同步衰微为特征,也就是以细胞 早衰老化为病理特征。脾虚气滞证胃病是癌前疾病,Ⅱb型肠化生是癌前病变。 相似文献
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大骨节病区和常硒及低硒非病区儿童发铜锌锰铁含量测定 总被引:2,自引:0,他引:2
采用原子吸收光谱法测定了大骨节病区、常硒非病区和低硒非病区儿童发Cu、Zn、Mn、Fe含量。结果表明,低硒非病区组儿童发Cu、Zn、Mn、Fe含量与常硒非病区组无明显差异(P>0.05),大骨节病区组儿童除发Cu含量与常硒、低硒非病区无显著性差异(P>0.05)外,发Zn、Mn、Fe含量均明显高于常硒、低硒非病区组(P<0.01或0.05),大骨节病区组儿童硒以外某些微量元素状况与常硒、低硒非病区 相似文献
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消化系癌血清微量元素与T细胞亚群相关性研究的价值 总被引:5,自引:3,他引:2
目的探讨消化系癌血清微量元素与T细胞亚群关系的临床意义.方法用原子吸收分光光度法测定食管癌312例和健康人100例血清Zn,Cu,Fe,Mn,Ca.48例癌患者和健康人外周血T细胞亚群用间接免疫荧光法测定.结果消化系癌患者血清中Cu含量,Cu/Zn比,CD8+显著高于健康人(P<001);Zn,Ca含量,CD3+,CD4+,CD4+/CD8+比值明显低于健康人(P<005~P<001);Ⅲ~Ⅳ期及转移癌患者Cu含量,Cu/Zn比,CD8+明显高于Ⅰ~Ⅱ期癌患者(P<005~P<001).直线相关分析表明:血清Cu,Cu/Zn比值与CD4+,CD4+/CD8+比成负相关,Zn与CD4+/CD8+比成正相关(P<005).多因素Logistic回归分析表明,Cu,Cu/Zn比升高,发生消化系癌的相对危险度升高;Zn,Ca含量升高,发生消化系癌相对危险度降低.结论对消化系癌患者适量补Zn,调节和改善宿主抗肿瘤免疫力;Cu/Zn比对消化系癌阳性诊断率为70%,特异性为73%.因此,Cu/Zn值可做为消化系癌的一项诊断指标,并对从高危人群中筛检消化系癌患者有一定的意义 相似文献
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脾肾阴虚证血清氧自由基损伤初探 总被引:2,自引:0,他引:2
目的从氧自由基损伤及机体抗氧化能力方面探讨脾阴虚证、肾阴虚证患者的客观病理学改变.方法观察脾阴虚组30例,肾阴虚组16例和健康人对照组16例.取受检者清晨空腹静脉血分离血清后分别测定:总SOD,Cu,ZnSOD(黄嘌呤氧化酶法);MDA(TBA法);总抗氧化能力(比色法).VitC(2,4DNPH比色法);VitE(高效液相色谱法);Cu,Zn(原子吸收法);数据以均数±标准差表示.用单因素方差分析,Schefe法作两两比较,P<005为显著性检验界值.结果总SOD及Cu,ZnSOD肾阴虚组低于脾阴虚与健康人组(F=1769和3032,P<005).脾阴虚组和肾阴虚组MDA高于健康人组(F=491,P<005),而总抗氧化能力却低于健康人组(F=5901,P<005).VitE肾阴虚组低于脾阴虚组和健康人(F=672,P<005).结论脾阴虚证、肾阴虚证患者都有一定程度的氧自由基损伤,但在形成的原因和机体防御消除的机制等方面是不同的 相似文献
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胃癌患者血清,组织和血细胞锌,铜,硒的含量变化 总被引:1,自引:0,他引:1
本文应用原子吸收检测技术对32例胃癌患者的血清、胃癌组织、红细胞、白细胞和血小板锌、铜、硒的含量进行检测,与胃炎组比较,结果:胃癌组血清锌含量降低(P<0.01),而血清铜含量和血清Cu/Zn比值升高(P<0.01),血清硒含量降低(P<0.01);白细胞锌含量降低(P<0.01),白细胞铜无显著变化;红细胞、血小板锌、铜、硒含无显著变化;胃癌组织中锌、硒含量增加(P<0.05),而铜含量无显著变化;锌、铜、硒及血清Cu/Zn比值与胃癌细胞分化程度以及有无肿转移无相关性。测定胃癌患者血清、组织、白细胞锌、铜、硒含量,可以估价体内这些元素的总体状态,为观察病情、估计预后提供依据。 相似文献
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不同病理类型胃息肉的细胞DNA定量研究 总被引:4,自引:0,他引:4
为探讨不同病理类型胃息肉的DNA生物学行为及其与胃癌的关系,应用流式细胞术对57例正常胃粘膜、胃息肉和胃癌组织作细胞DNA定量分析。正常胃粘膜、炎性息肉、增生性息肉、腺瘤性息肉及胃癌中,DNA非整倍体检出率分别为0%、7.7%、9.1%、36.4%和58.3%。各型胃息肉和胃癌细胞增殖指数(PI)均显著地高于正常胃粘膜(P<0.01);增生性息肉组和炎症性息肉组PI低于胃癌组,差异有显著性(P<0.05)或非常显著性意义(P<0.01),而腺瘤性息肉组PI与胃癌组近似(P>0.05)。结果表明胃息肉是一种细胞增殖活跃性病变,其中腺瘤性息肉的DNA生物学行为更接近于胃癌,可能容易癌变。 相似文献
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肝硬化和肝癌患者体内铜含量变化及其临床意义 总被引:5,自引:0,他引:5
采用原子吸收分光光度法等对肝硬化和肝癌患者体内铜含量(Cu)和血清铜蓝蛋白(Cp)进行了测定。结果表明:①肝硬化患者血清Cp和尿Cu高于正常人(P<0.05和0.01)。②肝癌患者血清Cu与Cp呈一致性升高;尿Cu明显升高且与血请Cu呈正相关(r=0.567,P<0.01)。③硬化肝Cu与癌周组织(有肝硬化改变)相近并高于正常肝和肝癌组织。④肝硬化和肝癌组头发Cu均明显低于对照组(P<0.01)。⑤大肝癌患者血清Cu明显高于小肝癌患者(P<0.05)。⑥治疗后肝癌缩小与血清Cu降低相一致。⑦血清Cu和Cp可作为诊断肝癌、特别是甲胎蛋白(AFP)阴性肝癌的辅助指标,前者亦可作为判断疗效的参考指标。 相似文献
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目的:观察胃康复冲剂治疗胃粘膜肠化生(IM)和不典型增生(ATP)的临床疗效。方法:中西医证病结合,辨证施治,随证加减应用胃康复冲剂作为治疗组(61 例);不进行辨证浑用胃苏冲剂作为对照组(54例)。治疗前后均作胃镜检查,取胃窦部粘膜作病理诊断和组织化学染色IM 分型。结果:治疗组显效率和总有效率分别为52.46% 、90.16% ,对照组为37.04% 、85.15% ,两组显效率有显著性差异(P< 0.05)。治疗组对不同类型胃粘膜癌前病变的治疗显效率和总有效率均高于对照组(P< 0.05~0.01)。结论:胃康复冲剂的疗效明显优于胃苏冲剂。 相似文献
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HBV 侵犯PBMC致线粒体功能改变 总被引:1,自引:0,他引:1
目的探讨HBV侵犯外周血单个核细胞(PBMC)后对其线粒体功能的影响.方法HBsAg阳性6个月以上,无肝炎症状及体征,肝功正常患者58例.多聚酶链反应检测PBMC中HBVDNA,噻唑兰(MTT)比色法测线粒体功能.HBsAg、HBeAg检测用固相放免法检测.结果慢性HBV感染者58例,PBMC中检出HBVDNA31例(535%),PBMC中HBVDNA阳性组MTT比色法查线粒体功能的A(OD500nm)值明显低于HBVDNA阴性组(005±003vs029±007,P<001).结论慢性HBV感染时,HBV常侵犯PBMC,并导致PBMC线粒体功能降低、能量代谢异常.这可为HBV慢性感染免疫功能异常的原因之一. 相似文献
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Distribution of gasses to the cast volume and volume of pores can be maintained within the acceptable limits by means of correct setting of technological parameters of casting and by selection of suitable structure and gating system arrangement. The main idea of this paper solves the issue of suitability of die casting adjustment—i.e., change of technological parameters or change of structural solution of the gating system—with regards to inner soundness of casts produced in die casting process. Parameters which were compared included height of a gate and velocity of a piston. The melt velocity in the gate was used as a correlating factor between the gate height and piston velocity. The evaluated parameter was gas entrapment in the cast at the end of the filling phase of die casting cycle and at the same time percentage of porosity in the samples taken from the main runner. On the basis of the performed experiments it was proved that the change of technological parameters, particularly of pressing velocity of the piston, directly influences distribution of gasses to the cast volume. 相似文献
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目的本文旨在了解医务人员现代结控知识掌握的现状及培训效果?方法于培训前后进行问卷调查,内容包括:病例发现?结核病诊断及化疗?结果培训前疫情报告和转诊,回答正确者占75.2%?71.7%;对临床表现?查痰和诊断依据,回答正确者占83.5%?42.5%?40.8%;抗痨药物?用药方法?化疗原则?短化方案?短化疗程?治愈标准六项,回答正确者占58%?14.4%?20.8%?9.2%?17%?24.3%?培训后再次调查发现,90%以上医务人员对现代结控基本知识已掌握?结论各级医务人员现代结控知识是很贫乏的,因此,对其进行系统培训是极为必要的,此项工作省时?省力?投入少,可收到事半功倍的效果。 相似文献
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John W. Braasch 《Journal of hepato-biliary-pancreatic sciences》1994,1(4):342-348
The historical evolution of the pylorus-preservation resection of the head of the pancreas is traced from the first resections early in this century to relative standardization of the operation, to a lowering of the operative mortality, and to an interest in improving nutritional status after resection. There are many theoretical advantages for the function of the upper gastrointestinal tract after pylorus and gastric preservation, such as maintenance of gastric capacitance and equilibration of osmotic pressure in gastric digestants, foodstuff digestion and absorption, and bowel motility. After the pylorus-preserving resection, gastric emptying is normal, pyloric function to prevent duodenal reflux is often normal, and gastric acids and serum levels of duodenal hormones are at normal levels, whereas after standard pancreatoduodenectomy, all of these are often abnormal. No prospective blinded studies have been published comparing nutritional values after the two operative procedures, but evidence is presented of a satisfactory result with regard to gastric capacitance, body weight gain, and lack of postgastrectomy symptoms. An undoubted advantage of the pylorus-preserving feature is a simplification of the operation. These gains are achieved without increase in operative mortality, without increase in the incidence of jejunal ulcer, and without theoretical or actual decrease in value of the procedure as a cancer operation, except in patients with duodenal carcinoma proximal to the ampulla of Vater. 相似文献
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Pylorus preservation has been advocated to decrease the morbidity associated with the classical or standard pancreaticoduodenectomy. The proposed advantages are decreased incidence of peptic ulceration, dumping syndrome, and nutritional problems. However, after an initial period of enthusiasm for the procedure, it is now being found that marginal ulceration at the duodenojejunal anastomosis is encountered with increasing frequency. Delay in gastric emptying occurs frequently, with an overall incidence of 30%. With the availability of better pancreatic enzyme supplements, the current incidence of nutritional problems and weight loss after the standard Whipple procedure is unknown. Whether there is a difference in long-term survival after the two procedures performed for adenocarcinoma of the head of the pancreas is still debatable. A controlled trial is needed to answer many of these questions, and pylorus-preserving pancreaticoduodenectomy should be used cautiously until further data become available. 相似文献
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