富血小板纤维蛋白的临床应用

富血小板纤维蛋白(PRF)是一种免疫和血小板聚集物,包含了所有能促进伤口愈合和增强免疫反应的成分。属于新一代的血小板聚集物,处理简单,不需要任何生化处理。在纤维蛋白结构中聚集了大量的血小板和白细胞,这种结构有利于细胞迁徙,促进细胞的增殖和分化。该文就PRF的发展过程、生物学特性以及临床应用做一综述。     

富血小板纤维蛋白应用于口腔组织再生研究进展

富血小板纤维蛋白（platelet-rich fibrin,PRF）是自体来源的富含白细胞、血小板的纤维蛋白生物材料,为第二代血小板浓缩物。PRF中含有多种生长因子,可为参与组织再生的间充质干细胞提供生物性的生长环境;此外,其纤维成分也可起到生物支架作用。文章对PRF应用于口腔组织再生的研究进展做一综述。     

富血小板纤维蛋白的研究进展

富血小板纤维蛋白(Platelet-Rich Fibrin, PRF)是一种富含细胞因子和生长因子的自体来源的新型生物材料,被誉为新一代血小板浓缩物。本文就PRF的生物学特点及其医学研究作用作一综述。     

富血小板纤维蛋白超微结构的观察与探讨

目的：观察富血小板纤维蛋白的显微和超微结构并探讨其相关生物学特性。方法：实验组6名健康志愿者,随机分为3组,采取肘静脉全血制备6份PRF标本,分别行光镜、SEM和TEM观察。采集对照组2名志愿者静脉全血制备PRP进行SEM观察。结果：PRF中纤维蛋白聚集形成疏松的立体网络结构,大量的血小板和白细胞分布于纤维蛋白网中,血小板内a颗粒部分破裂。PRP中血小板伸出大量的伪足并且聚集成簇相互堆叠,少量白细胞散在。结论：PRF的纤维蛋白网状结构是其相对延长生长因子作用时间的结构基础,所含的大量白细胞可能具有很高的免疫学价值。     

富血小板血浆促进软组织损伤愈合的研究进展

富血小板血浆是全血经过离心获得的血小板浓缩物,已有研究证实其中含有多种与创伤愈合有关的生长因子。软组织损伤理想的愈合方式是快速及疤痕最少的愈合,近年来,有关富血小板血浆促进软组织损伤愈合、减少疤痕的研究逐渐增多,已成为医学研究的热点,本文就其促进软组织损伤愈合的机制及最新研究进展作一综述。     

富血小板纤维蛋白体外释放TGF－β1浓度的探讨

目的：通过检测富血小板纤维蛋白（PRF）中转化生长因子－β1的浓度,了解PRF体外释放效用因子TGF－β1的趋势。方法：兔耳中央动脉抽血,离心3000 r／min×10 min,制取PRF膜,置于5 mL新鲜DMEM溶液中,分别于37℃下静置7 d、14 d、21 d、28 d收集析出液。ELISA法检测其浓度。结果：PRF析出液中TGF－β1质量浓度随着时间的递增而增大,至28 d时达到高峰,TGF－β1质量浓度在21～28 d时呈现一个显著增高的趋势。结论：不同时间对PRF析出液中TGF－β1的质量浓度变化存在一定的影响,随着时间的增加,TGF－β1的浓度逐渐增大。     

富血小板血浆与富血小板纤维蛋白在牙周组织再生中的应用

富血小板血浆(platelet-rich plasma,PRP)与富血小板纤维蛋白(platelet-rich fibrin,PRF)因其促进组织再生的能力,在临床口腔创伤及缺损的修复中逐渐被重视及应用,并取得了令人较为满意的效果,鉴于PRP与PRF的制作较简便,且不易出现疾病传染及免疫排斥的不良反应,两者在口腔临床中...     

富血小板纤维蛋白在口腔软硬组织再生中的作用

［摘要］富血小板纤维蛋白(platelet-rich fibrin, PRF)是利用自体血提取制备的血小板浓缩制品,因其具有三维立体结构且富含血小板及高浓度的生长因子,被视为一种促进组织愈合的生物材料,近年来已在口腔软硬组织缺损修复中广泛应用。本文对PRF在口腔软硬组织再生中的作用进行论述,客观评判其疗效,为口腔医师正确应用PRF提供参考依据。     

富血小板血浆与富血小板纤维蛋白在牙周组织再生中的应用

富血小板血浆（platelet-rich plasma,PRP）与富血小板纤维蛋白（platelet-richfibrin,PRF）因其促进组织再生的能力,在临床口腔创伤及缺损的修复中逐渐被重视及应用,并取得了令人较为满意的效果,鉴于PRP与PRF的制作较简便,且不易出现疾病传染及免疫排斥的不良反应,两者在口腔临床中的应用越来越广泛。牙周炎是目前造成牙周骨缺损最为常见的疾病之一,国内外的学者们为了探寻促进牙周组织再生的方法,将PRP与PRF用于牙周治疗中,本文即将近年来有关PRP与PRF在牙周组织再生中的临床应用作一综述。     

富血小板血浆与富血小板纤维蛋白联合冻干法研究进展

手术使用的浓缩血小板是再生医学的创新材料,富血小板血浆(PRP)是第一代富含血小板的浓缩物,已广泛应用于促进软硬组织愈合。富血小板纤维蛋白(PRF)是由法国学者Choukroun等首次提出的新一代血小板浓缩物,较传统富血小板血浆更具有应用优势。冻干法是保存生物制品的常规方法,也是控制支架空隙大小并保存其原有成分的有效手段。本文就于富血小板血浆和富血小板纤维蛋白结合冻干法应用于组织发育与再生作一综述。     

富血小板纤维蛋白的研究及在组织修复中的应用

富血小板纤维蛋白(PRF)是含有丰富的细胞因子和生长因子等类似天然血凝块的浓缩体。因有助于修复组织缺损,在实验和临床应用研究中得到了广泛的认可。该文就PRF的制备、生理特性、组织修复的应用等研究进展作一综述。     

The use of leukocyte- and platelet-rich fibrin in the management of soft tissue healing and pain in post-extraction sockets: A randomized clinical trial

This study aimed to evaluate the clinical effect of leukocyte- and platelet-rich fibrin (L-PRF) to improve epithelialization and decrease postoperative pain in post-extraction sockets. Thirty two participants requiring extractions of posterior teeth were randomized into two groups: 1) extractions and socket filling with L-PRF membrane (test group) and 2) extraction with spontaneous healing (control group). One week after extraction, an assessment of soft tissue healing around the sockets was performed using the healing index. Also, postoperative pain by visual analog scale (VAS) and number of consumed analgesic tablets were recorded. In the first week, the sockets of the test group presented a significantly (mean of 3.81 ± 0.54; p = 0.0138) higher level of healing when compared to the sockets of the control group (mean of 3.18 ± 0.65). The participants of control group reported a significantly (mean of 5.12 ± 1.08; p = 0.0128) higher level of postoperative pain when compared to the test group (mean of 4 ± 1.15). Also, the control group consumed a greater number of analgesics (mean of 1.75 ± 0.85; p = 0.0136) when compared to the test group (mean of 1 ± 1.15). The results of the present study demonstrate that whenever improved healing of the extraction socket is needed, the use of L-PRF should be considered. In addition, the use of L-PRF decreases postoperative pain and discomfort.     

The role of titanium-prepared platelet-rich fibrin in palatal mucosal wound healing and histoconduction

Objective: We aimed to determine the clinical effects of titanium-prepared, platelet-rich fibrin (T-PRF) on human palatal mucosal wound healing (PMWH) and to identify its effect on time-dependent changes in palatal soft-tissue thickness (PSTT) in terms of histoconduction, which is a new concept.

Materials and methods: Free gingival graft (FGG) donor sites were treated with T-PRF and compared with an untreated control group. The results of colour match and H₂O₂-bubbling tests for complete wound epithelization (CWE) were recorded on days 3, 7, 14 and 21. Pain level, number of analgesics used and bleeding status were recorded for the first 7 days. PSTT was measured at baseline and after 1 and 6 months.

Results: Colour match scores of the test group were significantly higher than those of the control group at 7 and 14 days. CWE was observed at a higher frequency in the test group than in the control group on day 14. Post-operative bleeding prevalence was lower in the test group than in the control group for the first 2 days. A time-dependent decrease in PSTT was observed at 1 and 6 months in the control group compared with baseline (baseline, 4.23?±?0.62?mm; 1 month, 4.01?±?0.68?mm; and 6 months, 3.93?±?0.69?mm). However, no significant difference was found in the test group (baseline, 4.29?±?0.64?mm; 1 month, 4.61?±?0.51?mm; and 6 months, 4.51?±?0.58?mm).

Conclusion: The T-PRF membrane exhibited positive effects on PMWH. T-PRF, which is a promising autogenous matrix for histoconduction, may also be preferred as an autogenous alternative to connective tissue grafts in the treatment of gingival recessions and peri-implant mucosal recessions.     

Randomized double-blind clinical trial evaluation of bone healing after third molar surgery with the use of leukocyte- and platelet-rich fibrin

The objective of this study was to evaluate the use of leukocyte- and platelet-rich fibrin (L-PRF) in bone healing after mandibular third molar extraction. In this prospective, double-blind, split-mouth study, 34 extractions were performed. On one side, the socket was sutured primarily (control side); on the other side, L-PRF was inserted before suturing. The patients were assessed for postoperative bone regeneration, pain and soft tissue healing. The primary outcome was bone regeneration, which was performed through tomographic evaluation in the immediate postoperative period and 3 months after the procedure. The ITK-SNAP software was used for image evaluation by the intensity of grey of each voxel. Pain was analysed using a visual analogue scale (VAS), and soft tissue healing was analysed both based on the modified healing index of Landry et al., and by comparing pre- and postoperative periodontal probing at the distal of the lower second molar. The application of L-PRF improved bone density, which was higher in test group (p = 0.007). There was no statistical difference related to pain or soft tissue between the groups (p > 0.05). There was evidence for improved bone healing in response to L-PRF. However, to better understand the effect of L-PRF more clinical trials with larger samples are necessary.     

Standardization of relative centrifugal forces in studies related to platelet-rich fibrin

Platelet-rich fibrin (PRF), a second-generation platelet concentrate, has been the focus of intensive research endeavors over the past 2 decades. Over the years, however, numerous reports have inaccurately reported relative centrifugal force (RCF) values, which has caused considerable confusion in the field. Furthermore, the use of trade names such as leukocyte and platelet-rich fibrin (L-PRF) and advanced platelet-rich fibrin (A-PRF) has further confused many readers, since studies have not always used centrifugation parameters with equal rotor sizes, angulation of tubes, and/or tube design. This has led to considerable misperception in the report of relative centrifugal force. Herein is described necessary parameters pivotal for the future report of RCF in studies related to PRF, which include: 1) dimensions of the rotor (radius at the clot and end of the tube); 2) rotor angulation for the tube holder; 3) revolutions per minute (RPM) and time; 4) RCF value calculated at either the RCF-minimum, RCF-clot, or RCF-maximum; 5) composition and size of tubes used to produce PRF; and 6) centrifugation model used. This editorial aims to minimize confusion in the field and create more transparent research reporting RCF values in future studies.     

富血小板纤维蛋白的研究进展

富血小板纤维蛋白(PRF)是一种富含细胞因子和生长因子的自体来源的新型生物材料,被誉为新一代血小板浓缩物。其分子结构类似天然血凝块,为组织细胞提供迁移、增殖和分化的场所。近来,许多学者将PRF作为移植材料应用于口腔种植前上颌窦底提升术后的骨移植中,并获得了良好的效果。本文就PRF及其在口腔种植上颌窦底提升术中的应用进展作一综述。     

Improvement in the repair of defects in maxillofacial soft tissue in irradiated minipigs by a mixture of adipose-derived stem cells and platelet-rich fibrin

To find out if adipose-derived stem cells (ASC) and platelet-rich fibrin (PRF), alone or combined, had any effect on the repair of maxillofacial soft tissue defects in irradiated minipigs, ASC were isolated, characterised, and expanded. Twenty female minipigs, the right parotid glands of which had been irradiated, were randomly divided into 4 groups of 5 each: those in the first group were injected with both ASC and PRF (combined group), the second group was injected with ASC alone (ASC group), the third group with PRF alone (PRF group), and the fourth group with phosphate buffer saline (PBS) (control group). Six months after the last injection, the size and depth of each defect were assessed, and subcutaneous tissues were harvested, stained with haematoxylin and eosin, and examined immunohistologically and for apoptosis. Expanded cells were successfully isolated and identified. Six months after injection the defects in the 3 treated groups were significantly smaller (p<0.001) and shallower (p<0.001) than those in the control group. Those in the combined group were the smallest and shallowest. Haematoxylin and eosin showed that the 3 treated groups contained more subcutaneous adipose tissue than the control group, and also had significantly greater vascular density (p<0.001) and fewer apoptotic cells (p<0.001). Both ASC and PRF facilitate the repair of defects in maxillofacial soft tissue in irradiated minipigs, and their combined use is more effective than their use as single agents.     

富血小板纤维蛋白衍生物的研究进展

富血小板血浆和富血小板纤维蛋白（PRF）目前已成熟地应用于口腔医学领域,随着制备技术与基础研究的不断深入,PRF的衍生物也相继出现并应用于口腔临床中。本文就PRF衍生物的演变、制备技术、生物学特性及医学研究进展作一综述。