共查询到20条相似文献,搜索用时 78 毫秒
1.
摘 要 目的:建立HPLC波长切换法同时测定茵栀祛黄胶囊中栀子苷、甘草苷、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量。方法: 采用Waters Sunfire C18(250 mm×4.6 mm,5 μm)色谱柱;流动相:甲醇(A) 0.05%磷酸溶液(B)(梯度洗脱),流速为1.0 ml·min-1 ,柱温为25℃,检测波长(0~15 min:在238 nm波长下检测栀子苷和甘草苷;15~50 min:在254 nm波长下检测芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚),进样量为10 μl。结果: 栀子苷、甘草苷、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的线性范围分别为0.13~3.18 μg·ml-1(r=0.999 8)、0.19~4.84 μg·ml-1(r=0.999 7)、0.28~7.02 μg·ml-1(r=0.999 9)、0.13~3.16 μg·ml-1(r=0.999 9)、0.61~15.27 μg·ml-1(r=0.999 9)、0.32~8.03 μg·ml-1(r=0.999 9)、0.39~9.81 μg·ml-1(r=0.999 9);平均加样回收率分别为98.84%(RSD=0.74%)、99.34%(RSD=0.86%)、99.54%(RSD=0.30%)、99.56%(RSD=0.80%)、99.85%(RSD=0.41%)、99.57%(RSD=0.70%)、99.64%(RSD=0.30%)(n=9)。结论: 本文建立的HPLC含量测定方法,具有操作简便、专属性高、重复性良好、结果准确可靠的特点,可用于茵栀祛黄胶囊的质量控制。 相似文献
2.
摘 要 目的: 建立尿塞通片中4个活性成分丹酚酸B、羟基红花黄色素A、芍药苷和橙皮苷含量同时测定的方法。方法: 采用Phenomsil C18色谱柱,以乙腈-0.1%磷酸为流动相,梯度洗脱;流速:1.0 ml·min-1;检测波长230 nm,柱温30℃。结果: 丹酚酸B、羟基红花黄色素A、芍药苷、橙皮苷分别在20.056~150.420μg·ml-1(r=0.999 5),6.068~45.510 μg·ml-1(r=0.999 5),5.052~37.890μg·ml-1(r=0.999 7),15.328~114.960μg·ml-1(r=0.999 6)的浓度范围内线性关系良好,平均回收率分别为98.86%、99.40%、98.21%、98.25%,RSD分别为1.07%、0.85%、1.35%、1.18%(n=6)。结论:本法简便、准确、重复性好,可用于尿塞通片的质量控制。 相似文献
3.
摘 要 目的:建立多波长切换高效液相色谱法同时测定皮肤康洗液中芍药苷、甘草苷、甘草酸铵、大黄素和蛇床子素5种有效成分的含量。方法: 色谱柱为 Diamonsil C18柱(200 mm×4.6 mm,5 μm);流动相为乙腈 0.1%盐酸水溶液,梯度洗脱;流速为1.5 ml·min-1;柱温为30℃;检测波长为232 nm(0~6 min,检测芍药苷)、277 nm(6~10 min,检测甘草苷)、254 nm(10 min以后,检测甘草酸铵、大黄素、蛇床子素)。结果: 芍药苷、甘草苷、甘草酸铵、大黄素和蛇床子素的线性范围分别为28.20~282.0 μg·ml-1(r=0.999 7)、12.150~121.500 μg·ml-1(r=0.998 8)、13.420~134.200 μg·ml-1(r=0.999 5)、0.047~0.466 μg·ml-1(r=0.999 9)、2.380~23.800 μg·ml-1(r=0.999 9),平均加样回收率分别为98.49%,99.00%,98.38%,97.36%,97.70%,RSD分别为0.71%,0.62%,0.85%,0.92%,0.78%(n=6)。结论:该方法简便、准确、灵敏度高、重复性好,可用于同时测定皮肤康洗液中上述5个有效成分的含量。 相似文献
4.
摘 要 目的:建立HPLC法同时测定玉真散中天麻素、升麻素苷、5-O-甲基维斯阿米醇苷、欧前胡素、异欧前胡素含量。方法: 色谱柱为Phenomenex Gemini C18柱(250 mm×4.6 mm,5 μm),柱温为25℃,流速为1.0 ml·min-1 ,流动相为甲醇 水梯度洗脱,检测波长为230 nm,进样量为5 μl。结果: 天麻素在2.68~214.00μg·ml-1范围内线性关系良好(r=0.999 9),平均回收率为100.2%,RSD为0.9%(n=6);升麻素苷在5.22~418.00 μg·ml-1范围内线性关系良好(r=0.999 7),平均回收率为99.9%,RSD为0.9%(n=6);5-O-甲基维斯阿米醇苷在4.57~365.80 μg·ml-1范围内线性关系良好(r=0.999 5),平均回收率为99.7%,RSD为1.0%(n=6);欧前胡素在5.22~417.20μg·ml-1范围内线性关系良好(r=0.999 6),平均回收率为99.0%,RSD为0.9%(n=6);异欧前胡素在5.29~423.20μg·ml-1范围内线性关系良好(r=0.999 8),平均回收率为100.2%,RSD为0.8%(n=6)。结论: 该方法简单、准确,可同时测定5种成分的含量,可用于玉真散的质量控制。 相似文献
5.
摘 要 目的: 建立HPLC法同时检测大败毒胶囊中绿原酸、咖啡酸和阿魏酸的测定方法。方法: 采用Agilent C18(250 mm×4.6 mm,5 μm)色谱柱;乙腈-0.4%磷酸溶液(15∶85)为流动相,流速:1.0 ml·min-1;检测波长:324 nm。柱温:30℃,进样量:10 μl。结果:线性范围:绿原酸5.42~32.43 μg·ml-1(r=0.999 8),咖啡酸2.63~26.32 μg·ml-1(r=0.999 4),阿魏酸1.44~14.44 μg·ml-1(r=0.999 9);平均回收率:绿原酸99.98%,RSD=0.2%(n=6),咖啡酸99.31%,RSD=0.4%(n=6),阿魏酸99.48%,RSD=0.8%(n=6)。结论:该方法操作简便、准确,重复性好,可用于测定大败毒胶囊中绿原酸、咖啡酸和阿魏酸的含量。 相似文献
6.
摘 要 目的: 建立高效液相色谱法同时测定枳实消痞丸中芸香柚皮苷、柠檬苦素、和厚朴酚及厚朴酚含量的分析方法。方法: 色谱柱为岛津Shim-pack VP-ODS C18柱(250 mm×4.6 mm,5 μm),流动相A为乙腈 甲醇(1∶2),流动相B为水,梯度洗脱,流速为1.0 ml·min-1,柱温为30℃,进样量为20 μl;芸香柚皮苷检测波长为λ1=283 nm,柠檬苦素检测波长为λ2=210 nm,和厚朴酚、厚朴酚检测波长为λ3=294 nm。结果: 芸香柚皮苷、柠檬苦素、和厚朴酚、厚朴酚分别在5.26~105.20μg·ml-1(r=0.999 8)、7.65~153.00 μg·ml-1(r=0.999 4)、6.21~124.20 μg·ml-1(r=0.999 3)、6.45~129.00 μg·ml-1(r=0.999 6)范围内线性关系良好,平均加样回收率(n=6)分别为99.00%(RSD=0.77%)、98.17%(RSD=1.19%)、98.78%(RSD=0.86%)、97.90%(RSD=0.99%)。结论:该方法简便、准确、可靠,可用于枳实消痞丸的质量控制。 相似文献
7.
摘 要 目的: 提高黄连上清片质量标准,建立同时测定绿原酸、栀子苷、黄芩苷、盐酸小檗碱4种成分含量的方法。方法: 采用高效液相色谱法,色谱柱为DiamonsilTM C18(250 mm×4.6 mm,5 μm),流动相为二元梯度系统,其中溶剂A为乙腈,溶剂B为0.3%磷酸水溶液,流速为1.0 ml·min-1,检测波长为238 nm,柱温30℃,进样量为10 μl。结果: 绿原酸、栀子苷、黄芩苷、盐酸小檗碱的线性范围分别为8.11~81.10 μg·ml-1(r=0.999 7)、13.08~130.80 μg·ml-1(r=0.999 7)、10.76~107.60 μg·ml-1(r=0.999 8)、7.92~79.20 μg·ml-1(r=0.999 8)范围内呈良好的线性关系,平均加样回收率分别为99.19%(RSD=0.9%)、98.44%(RSD=1.1%)、99.12%(RSD=1.0%)、99.18%(RSD=1.1%)(n=9)。结论:该方法简便、准确、重复性好,可用于黄连上清片的质量控制。 相似文献
8.
摘 要 目的:建立双波长HPLC法同时测定复方肤清洗剂中绿原酸、咖啡酸、芍药苷3种指标性成分含量的方法。方法: 采用Inertsil C18柱(250 mm×4.6 mm,5 μm)为色谱柱,柱温40℃,以乙腈-0.02%磷酸水溶液(17∶83)为流动相,流速为1.0 ml·min-1,检测波长分别为323 nm、230 nm。结果:绿原酸、咖啡酸和芍药苷分别在7.50~120.00 μg·mL-1,2.50~40.00 μg·mL-1,14.06~225.00 μg·mL-1范围内线性关系良好,r分别为0.999 9,0.999 8,0.999 7;平均加样回收率分别为98.55%,94.52%,99.18%,RSD分别为1.66%,0.98%,0.65%(n=6)。结论:该方法简便快捷、结果准确、专属性强,可用于复方肤清洗剂的质量分析与控制。 相似文献
9.
摘 要 目的:建立利用HPLC法采取波长变换同时测定小儿咳喘灵颗粒中盐酸麻黄碱、(R,S) 告依春、苦杏仁苷、绿原酸、甘草苷、甘草酸含量的方法。方法: 色谱条件:Agilent Eclipse XDB C18(250 mm×4.6 mm,5 μm),以乙腈 0.1%磷酸为流动相,梯度洗脱,流速为1.0 ml·min-1 ,测定波长为207 nm(盐酸麻黄碱、苦杏仁苷)、237 nm(甘草酸、甘草苷、绿原酸)、245 nm[(R,S) 告依春)]。结果:盐酸麻黄碱进样浓度在2.423~96.920 μg·m-1 范围与峰面积线性关系良好,r=0.999 2,平均回收率为100.1%(RSD=0.30%,n=6);(R,S) 告依春进样浓度在1.920~76.798 μg·m-1 范围与峰面积线性关系良好,r=0.999 9,平均回收率为99.86%(RSD=1.14%,n=6);绿原酸进样浓度在2.396~92.891 μg·m-1 范围与峰面积线性关系良好,r=0.999 9,平均回收率为98.57%(RSD=0.75%,n=6);苦杏仁苷进样浓度在1.982~79.279 μg·m-1 范围与峰面积线性关系良好, r=0.999 8,平均回收率为99.67%(RSD=0.59%,n=6);甘草苷进样浓度在2.136~85.440 μg·m-1 范围与峰面积线性关系良好, r=0.999 9,平均回收率为98.57%(RSD=0.69%,n=6);甘草酸进样浓度在2.432~97.260 μg·m-1 范围与峰面积线性关系良好,r=0.999 9,平均回收率为98.57%(RSD=0.11%,n=6)。结论:该方法准确、重复性好,可用于小儿咳喘灵颗粒的质量控制。 相似文献
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摘 要 目的:建立双波长高效液相色谱切换法同时测定祛白颗粒中定量指标二苯乙烯苷、阿魏酸及丹皮酚含量的方法。方法: 采用双波长高效液相色谱法,色谱柱: ODS (150 mm×4.6 mm,5 μm);流动相:甲醇:0.1%磷酸为流动相进行梯度洗脱,流速:1.0 ml·min-1;柱温:35℃,进样体积:10 μl,检测波长:丹皮酚为274 nm、阿魏酸和二苯乙烯苷为320 nm。结果:3种有效成分的线性范围分别为1.56~156.00 μg·ml-1(r二苯乙烯苷=0.999 8)、1.00~100.0 μg·ml-1(r阿魏酸=0.999 8),1.61~161.00 μg·ml-1(r丹皮酚=0.999 7);平均加样回收率100.33%~100.76%,RSD均小于2%(n=6)。结论:该含量测定方法简便易行、稳定可靠、专属性强,可用于祛白颗粒的质量控制。 相似文献
11.
C D Klaassen 《European journal of pharmacology》1973,23(3):270-275
The choleretic properties of cholic, chenodeoxycholic, and deoxycholic acid and their taurine and glycine conjugates were compared to their ability to form micelles. It has previously been concluded that deoxycholate has the lowest critical micellar concentration; chenodeoxycholate is slightly higher and cholic is much higher. Conjugation with glycine and taurine has little or no effect on the critical micelle concentration. Since the choleretic properties of bile salts are thought to be directly proportional to their osmotic activities, one might suspect that deoxycholic acid would be the least choleretic, chenodeoxycholic slightly more choleretic and cholic much more choleretic, with little difference between the conjugated and unconjugated forms. However, in the present study, cholic, chenodeoxycholic and taurocholic acid produced similar increases in bile flow (450–700 μl/kg) after an equimolar dose (55 μM/kg). Except for the conjugation of deoxycholic acid with taurine, conjugation of these bile acids with glycine or taurine always decreased the choleretic properties of the bile acids. Therefore, it has been concluded that there is not a good correlation between the in vitro osmotic properties of bile acids and their ability to increase bile flow. 相似文献
12.
从产于四川宜宾翼梗五味子果实中,分得抗癌活性成分满五酸(1),同时分得schizandronic acid(2)和cuparenic acid(3),结构由与标准化合物比较光谱数据和物理常数而确证。 相似文献
13.
Summary Four pure chemicals, ellagic acid (E), caffeic acid (C), luteolin (L) and punicic acid (P), all important components of the aqueous compartments or oily compartment of pomegranate fruit (Punica granatum), and each belonging to different representative chemical classes and showing known anticancer activities, were tested as potential inhibitors of in vitro invasion of human PC-3 prostate cancer cells in an assay employing Matrigel™ artificial membranes. All compounds significantly inhibited invasion when employed individually. When C, P, and L were equally combined at the same gross dosage (4 μ g/ml) as when the compounds were tested individually, a supradditive inhibition of invasion was observed, measured by the Kruskal-Wallis non-parametric test.An erratum to this article can be found at 相似文献
14.
[摘要]目的:建立高效液相色谱法测定熊去氧胆酸片有关物质含量的方法。方法:用十八烷基硅烷键合硅胶为填充剂,以磷酸盐缓冲液(pH3.0)-甲醇-乙腈(35:37:28)为流动相,示差折光检测器测定。结果:在选定的色谱条件下,各成分可达到很好的分离,7-酮基胆石酸、胆酸、鹅去氧胆酸、胆石酸分别在0.05~2.00,0.05~2.00,0.05~2.00,0.10~2.00mg·mL-1的范围内线性关系良好(r>0.9989);精密度、稳定性试验的RSD均不大于0.5%;回收率分别为100.1%、99.8%、100.7%和101.9%(n=9,RSD<1.4%)。7-酮基胆石酸、胆酸、鹅去氧胆酸、胆石酸定量限分别为3.0、2.5、7.0、20μg·mL-1,检测限分别为0.8、0.7、2.0、3.5μg·mL-1。测定供试品两批,供试品一7-酮基胆石酸检出量为0.03%,胆酸和胆石酸均未检出,鹅去氧胆酸检出量为0.66%;供试品二7-酮基胆石酸、鹅去氧胆酸检出量均为0.10%,胆酸和胆石酸均未检出。结论:该方法灵敏度高,专属性强,适于测定熊去氧胆酸片中有关物质的含量。 相似文献
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Shi Yong Ryu Sang Un Choi Seung Ho Lee Chong Ock Lee Zaesung No Jong Woong Ahn 《Archives of pharmacal research》1994,17(5):375-377
Thirteen kinds of naturally occurring or derivatised triterpenes, reported to have an antitumoral property, were reinvestigated
on the basis of their direct cytotoxicity or the inhibitory activity on cell growth against five kinds of cultured human tumor
cells,i. e., A-549, SK-OV-3, SK-MEL-2, XF498 and HCT15,in vitro. Ursonic acidIII, betulinic acidVIII, betulonic acidX and glycyrrhetinic acidXI were exhibited a marked inhibition on cell growth. 相似文献
18.
目的建立快速测定升麻中咖啡酸、阿魏酸及异阿魏酸含量的HPLC方法。方法色谱柱为C18柱(4.6 mm×250 mm,5μm);流动相:乙腈-0.01%磷酸梯度洗脱;柱温:30℃;检测波长:320 nm;流速:1.0 ml/min。结果咖啡酸、阿魏酸、异阿魏酸分别在5.78~115.6μg/ml(r=0.9998,n=6),4.03~80.60μg/ml(r=1,n=6),16.45~329.00μg/ml(r=1,n=6)范围内线性关系良好,回收率在97.6%~101.5%。结论建立的方法准确可靠,可以用于测定升麻中咖啡酸、阿魏酸、异阿魏酸的含量。 相似文献
19.
目的建立L-天门冬氨酸中羟基丁二酸、丁二酸和富马酸等有关物质的检查方法。方法采用HPLC法,使用AQ C18色谱柱,0.01 mol.L-1的磷酸二氢钾水溶液(磷酸调pH3)为流动相,检测波长200 nm。结果主成分和杂质可完全分离。结论建立的方法灵敏、专属,可用于L-天门冬氨酸中有关物质的测定。 相似文献
20.
Summary The antipyretic activity of three N-aryl-anthranilic acid derivatives, mefenamic acid, tolfenamic acid and flufenamic acid, was compared and their optimal antipyretic dose determined in a trial in 87 children (aged 5 months to 15 years), who suffered from infections and fever exceeding 38.5°C. Tolfenamic acid proved to be the most potent antipyretic agent of the three drugs; it was eight times more powerful than mefenamic acid and three times more powerful than flufenamic acid. The optimal antipyretic doses were: mefenamic acid 4 mg/kg, tolfenamic acid 0.5 mg/kg and flufenamic acid 1.5 mg/kg. It is evident that the antipyretic activity of these anthranilic acid derivatives is even greater than their antirheumatic effect, the difference being most noticeable in the case of tolfenamic acid. 相似文献