维生素C联合顺铂与单药顺铂对Hela细胞生长状况影响的比较

目的 探讨维生素C(VC)和顺铂(DDP)联合应用对人宫颈癌Hela细胞株的体外抑制作用.方法 将VC、DDP单独或联合应用于Hela细胞,用MTT法检测细胞增殖的抑制率,并于用药后应用激光共聚焦显微镜观察细胞形态学变化.结果 不同浓度的顺铂对细胞生长均有抑制作用,随药物浓度的增加,抑制作用加强.VC和DDP联合应用后...     

维生素C增加人宫颈癌Hela细胞株对顺铂敏感性的研究

目的研究维生素C与顺铂合用对人宫颈癌Hela细胞株增殖的影响。方法用MTT比色法检测细胞生长抑制作用,流式细胞术测定各组细胞周期分布和凋亡率。结果维生素C和顺铂均可抑制Hela细胞生长,将肿瘤细胞阻滞在G0/G1期并诱导细胞凋亡。两药联合作用时表现出协同作用,细胞生长抑制率显著提高,凋亡率明显增加。结论维生素C可与顺铂协同抑制人宫颈癌Hela细胞的增殖,其协同作用的机制可能与诱导细胞凋亡有关。     

维生素C增加入宫颈癌Hela细胞株对顺铂敏感性的研究

目的研究维生素C与顺铂合用对人宫颈癌Hela细胞株增殖的影响。方法用MTT比色法检测细胞生长抑制作用，流式细胞术测定各组细胞周期分布和凋亡率。结果维生素C和顺铂均可抑制Hela细胞生长，将肿瘤细胞阻滞在G0／G1期并诱导细胞凋亡。两药联合作用时表现出协同作用，细胞生长抑制率显著提高，凋亡率明显增加。结论维生素C可与顺铂协同抑制人宫颈癌Hela细胞的增殖，其协同作用的机制可能与诱导细胞凋亡有关。     

以Celecoxib为基础的联合方案对宫颈癌细胞抑制作用的研究

目的:探讨环氧化酶-2(cox-2)抑制剂Celecoxib与化疗药顺铂(DDP)联合应用对人宫颈癌Hela细胞的作用及机制。方法:用免疫细胞化学SP法检测Hela细胞cox-2蛋白表达后,将Celecoxib、DDP单独或联合处理Hela细胞,用MTT法检测细胞增殖的抑制率,流式细胞仪检测细胞周期及凋亡。结果:Celecoxib可下调Hela细胞cox-2蛋白表达,抑制其增殖作用呈浓度和时间依赖性。在一定浓度范围内DDP也抑制Hela细胞生长,作用呈量效关系。Celecoxib与DDP联合应用后抗增殖作用较单一用药显著增强(P<0.05),DDP浓度≥1 mg/L时两者有协同或相加作用。Celecoxib及DDP均可有效诱导Hela细胞凋亡,联用凋亡率明显增加并出现G0-G1期细胞阻滞。结论:Celecoxib与DDP联用可通过抑制增殖、诱导细胞凋亡和细胞周期阻滞对Hela细胞有协同抗肿瘤效应,提示两者联合可能在宫颈癌临床治疗上具有潜在价值。     

VEGF-C对宫颈癌细胞增殖和凋亡影响的研究

目的:探讨VEGF-C对宫颈癌Hela细胞增殖和凋亡的影响。方法:采用MTT比色法,检测不同浓度的VEGF-C对宫颈癌Hela细胞增殖的结果:及采用流式细胞仪检测VEGF-C联合顺铂(DDP)对宫颈癌Hela细胞凋亡的影响。结果:随着VEGF-C的浓度的逐渐升高,由50ng/ml、100ng/ml、150ng/ml、升至200ng/ml,Hela细胞的增殖率逐渐增加;不同浓度的VEGF-C预处理Hela细胞48h后,细胞的凋亡率依次下降。结论:VEGF-C能促进宫颈癌Hela细胞的增殖,抑制DDP诱导宫颈癌细胞Hela的凋亡。     

肿瘤坏死因子相关凋亡诱导配体和顺铂对Hela细胞的诱导凋亡作用

目的 观察不同浓度的肿瘤坏死因子相关凋亡诱导配体(TRAIL)和顺铂对Hela细胞的诱导凋亡作用.方法 采用不同浓度的TRAIL作用于Hela细胞24 h,TRAIL 100 ng/mL、顺铂10 μmol/L及两者联合作用于细胞24 h,应用ELISA方法检测细胞凋亡率.结果 不同浓度的TRAIL作用于细胞后,细胞凋...     

多西紫杉醇联合顺铂对人骨肉瘤细胞的增殖抑制作用

目的:研究多西紫杉醇联合顺铂对人骨肉瘤细胞的增殖抑制作用。方法:应用细胞计数、形态学观察、流式细胞术等方法检测和观察分别及联合应用多西紫杉醇和顺铂对人骨肉瘤细胞株的增殖抑制。结果:多西紫杉醇和顺铂分别及联合用药,在一定范围内均对骨肉瘤细胞有明显的增殖抑制和诱导凋亡作用,并且联合用药组作用明显强于单独用药组(抑制率≥59.34%,P<0.05;凋亡率18.31%,P<0.01)。结论:多西紫杉醇和顺铂联合用药比单独用药对骨肉瘤细胞株增殖抑制作用更强。     

丹参酮Ⅱ A联合顺铂对Hela细胞生长及p53蛋白表达水平的影响

目的:研究丹参酮Ⅱ A联合顺铂在体外对宫颈癌Hela细胞生长的影响及细胞中p53蛋白表达水平的变化。方法:采用MTT法测定丹参酮Ⅱ A联合顺铂对Hela细胞增殖的影响;HE染色法观察细胞的形态学变化;流式细胞仪检测药物作用后的细胞凋亡情况;Western Blot测定p53蛋白水平的表达。结果:丹参酮ⅡA联合顺铂用药组比同等剂量的两药单用组及对照组对Hela细胞的增殖抑制率高(P<0.05);对照组细胞未见凋亡,而两药单用组及联合组均可见细胞凋亡;药物处理组均可检测到细胞亚G1峰,两药联合组细胞亚G1峰与单药组差异显著;在只含溶媒组、丹参酮Ⅱ A组、顺铂组及丹参酮Ⅱ A联合顺铂组中p53蛋白水平表达逐步升高。结论:丹参酮Ⅱ A联合顺铂比两药单用能显著抑制Hela细胞的生长;二者通过增加p53蛋白的表达,促进细胞凋亡。     

龙葵诱导宫颈癌Hela细胞凋亡及其机制研究

目的研究中药龙葵诱导人宫颈癌Hela细胞凋亡的作用及其机制,为临床用药提供相关依据。方法体外培养人宫颈癌Hela细胞株,不同浓度龙葵干预后,应用MTT法检测Hela细胞生长抑制率。结果龙葵对于宫颈癌Hela细胞的生长率具有一定的抑制作用,且其抑制作用的强弱与干预时间以及龙葵浓度呈正相关,其中以100μM浓度的龙葵干预72h最明显,与对照组相比有统计学差异(P0.05)。结论龙葵可增加宫颈癌Hela细胞的凋亡率,能够抗人宫颈癌Hela细胞的增殖,可以作为抗肿瘤的有效药物加强研究。     

姜黄素与顺铂联合对人肺腺癌A549细胞增殖和凋亡作用

目的:研究姜黄素、顺铂和两者联合应用对人肺癌A549细胞增殖、凋亡和细胞周期的影响。方法:采用MTT法观察姜黄素和顺铂对人肺癌A549细胞增殖的影响;采用流式细胞仪检测姜黄素、顺铂和两者联合应用对人肺癌A549凋亡和细胞周期的影响。结果:①姜黄素及顺铂能抑制人肺腺癌A549细胞的增殖,并呈浓度及时问依赖性;姜黄素10,15,20μmol/L分别与顺铂1,2 mg/L联合24,48,72 h的抑制率显著高于单用顺铂、姜黄素组(均为P<0.05),两者呈现出相加的抗瘤效果。②姜黄素和顺铂均可在一定程度上诱导细胞凋亡,并且凋亡率与浓度呈正相关。两种药物联用后,诱导细胞凋亡作用显著增强。③姜黄素将细胞周期阻滞在G2/M期(P<0.01),顺铂将细胞周期阻滞在S期(P<0.01)。结论:姜黄素、顺铂均可抑制人肺癌A549细胞的增殖、诱导细胞的凋亡,在一定浓度范围内,呈量-效关系,而且两者联合应用具有相加或协同作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.