当归多糖对人头皮毛囊体外培养的影响

目的:评价当归多糖对体外培养人头皮毛囊生长的影响。方法:观察不同浓度的当归多糖对体外培养人头皮毛发生长的影响。结果:低剂量组(62.5μg/mL、125μg/mL)在培养初期和末期加速毛发生长并可延长毛发的生长时间,中剂量组(250μg/mL、500μg/mL)仅在培养初期加速毛发生长而对毛发生长时间的影响不明显,高剂量组(1000μg/mL)在整个生长周期抑制毛发生长,同时缩短毛发生长时间。结论:低剂量当归多糖对体外培养的人头皮毛囊生长有促进作用。     

女贞子等对人头皮毛囊体外培养影响的研究

目的 研究中药对体外培养人头皮毛囊生长的影响。方法 观察不同浓度的野山楂、女贞子、猪苓、白芨提取物混合剂以及人参总皂甙对体外培养人头皮毛发生长的影响。结果 野山楂、女贞子、猪苓、白芨提取物混合剂低剂量组(1.28μg/mL和6.4μg/mL)在培养初期和末期加速毛发生长,32μg/mL在培养末期加速毛发生长。低剂量组(1.28μg/mL,6.4μg/mL,32μg/mL)可延长毛发的生长时间,尤以1.28μg/mL和6.4μg/mL组的延长作用更强;中剂量组(160μg/mL和800μg/mL)仅在培养初期加速毛发生长,而对毛发生长时间的影响不明显;高剂量组(4mg/mL和20mg/mL)在整个生长周期抑制毛发生长,同时缩短毛发生长时间。人参总皂甙高剂量组(40μg/mL和200μg/mL)抑制毛发的生长,缩短毛发的生长时间,且浓度越高,抑制作用越强,缩短作用越明显。结论 低剂量的野山楂、女贞子、猪苓、白芨提取物混合剂对体外培养的人头皮毛囊生长有促进作用。     

米诺地尔对培养人头皮毛囊生长的影响

通过毛囊器官培养模型及3H－TdR掺入实验观察不同浓度米诺地尔对人头皮毛囊生长的影响。结果：0．05mmol/L,0.1mol/L及0.5mmol/L米诺地尔组在加药后1－3天内毛囊平均每天生长长度和总生长长度均明显大于对照组（P＜0．01，P＜0．05），3H－TdR掺入实验救 射活性显著高于对照组，2mmol/L米诺地尔组毛囊生长速度显著受抑（P＜0．05），3个低浓度米诺地尔组毛囊组织学发生退行期改变的时间均较对照组延迟，米诺地尔在一定的浓度范围促进人头皮毛囊生长，对毛囊的正调节作用是直接的，但高浓度反而抑制毛囊生长。     

首乌、川芎、甘草、侧柏叶对体外培养人头皮毛囊的影响

目的探讨首乌、川芎等中药水提取物混合剂对体外培养人头皮毛囊生长的影响。方法将体外培养的人头皮毛囊分别设为对照组和不同浓度的中药组。显微镜下观察各组毛囊毛发生长情况。结果首乌、川芎、甘草、侧柏叶提取物混合剂中剂量组(100μg/mL和200μg/mL)可加速毛发生长,延长毛发生长长度和生长时间。高剂量组(1000μg/mL和2000/μgmL)抑制毛发生长,同时缩短毛发生长时间。低剂量组(2μg/mL和20μg/mL)对毛发生长无明显作用。结论中剂量首乌、川芎等提取物混合剂对体外培养的人头皮毛囊生长有促进作用。     

表皮生长因子对游离毛囊生长的影响

目的 通过毛囊器官培养观察表皮生长因子（ＥＧＦ）对人头皮毛囊生长的影响。方法 用Ｗｉｌｌｉａｍｓ Ｅ无血清培养基进行人头皮毛囊游离培养,分为２组,ＥＧＦ组（４３根）和对照组（４３根）。结果 ＥＧＦ ２０μｇ／Ｌ组在加药后１～３ｄ毛干平均生长０．２２ｍｍ／ｄ,且平均生长０．１６ｍｍ／ｄ,两组差异有显著性（Ｐ〈０．０５）,ＥＧＦ对毛囊生长有更明显的促进作用。但ＥＧＦ组毛囊于培养的第４～６天即出现退行期     

WilliamsE无血清培养基中培养人头皮毛囊

建立体外毛囊器官培养技术,有助于了解毛囊生物学特性,为探讨毛囊生长调节机制以及为脱发等相关疾病的治疗奠定基础。Ｐｈｉｌｐｏｔｔ等[1]在1990年已建立了人头皮游离毛囊培养的模型,并对一些细胞因子、皮质类固醇及药物对毛囊生长的影响进行了探讨。我国也已逐渐开展了这方面的工作,建立了游离毛囊培养的方法[2]。本试验采用ＷｉｌｌｉａｍｓＥ无血清培养基(含氢化可的松、牛胰岛素、转铁蛋白、亚硒酸钠、青霉素、链霉素)进行游离毛囊培养,对毛囊的形态变化、生长长度及生长时间进行了观察与检测。一、材料和方法1标本来源:18～35岁新鲜…     

硒与氟对体外培养的人头皮毛囊丙二醛水平的影响

目的观察离体培养条件下氟与硒对人头皮毛囊脂质过氧化物水平的影响。方法构建人头皮游离毛囊培养模型,在模型中,加入不同浓度的氟化钠和亚硒酸钠,筛选浓度后分为7组;用硫代巴比妥酸(th ibab ituric ac id,TBA)法测定毛囊培养基中丙二醛(MDA)含量,并进行统计分析。结果0.1 mmol/L氟化钠不能增加游离毛囊培养基中MDA含量,1 mmol/L和10 mmol/L的氟化钠能增加MDA含量;0.01 mmol/L的亚硒酸钠能拮抗1 mmol/L氟化钠的上述作用,但不能拮抗10 mmol/L氟化钠对MDA水平的影响。结论一定浓度的氟化钠能增加培养基中MDA的水平,0.01mmol/L亚硒酸钠能拮抗一定浓度的氟化钠对毛囊培养基MDA水平的影响。     

分离酶法体外分离和培养人毛囊黑素细胞

越来越多的研究发现,人毛囊黑素细胞(hair follicle melanocyte,HFM)在白癜风的色素恢复中起重要作用.1995年Tobin等^[1]首次报道胶原酶法体外分离培养人HFM成功,我们加以改进后发现分离酶法更易成功.这为更好地研究人HFM的生物学特性提供了重要基础.     

小鼠触须毛囊体外培养的研究

目的 为了建立较理想的小鼠触须毛囊体外培养模型,对不鼠触须毛囊体外培养方法进行了研究。方法 通过小鼠触须毛囊体外无血清培养基的方法,对不同鼠龄及不同生长周期的毛囊进行了研究。结果 ①毛囊在Ｗｉｌｌｉａｍｓ－Ｅ加Ｌ－谷酰胺培养基上生长平均达８天;②通过对比不同阶段的生长期毛囊发现生长期－Ⅱ毛囊生长力明显优于其它各期毛囊;③对不同鼠龄毛囊体外增培养生长状况的研究发现出生３５天和６５天鼠优于出生７天乳鼠     

人毛囊体外分离及培养技术的改进

在研究机体上皮的发生和创面愈合的实验模型中,毛囊是一个良好的观察器官和实验对象。从1990年Philpott1成功进行体外游离毛囊培养以来,毛囊的分离及培养技术延续至今一直没有很大的改进,我们在人游离毛囊体外培养试验中发现,现有实验方法存在一些不足并进行了改进,现提出与大家探讨。1单株毛囊分离前皮条的大小取皮后,在对头皮进行消毒后会首先将头皮修剪成合适大小的皮条,相关文献报道2,3皮条的大小为(3~5)mm×10mm,我们认为皮条的适宜宽度为3mm,当毛囊宽度适宜时可以获得更多的实验用单株毛囊且节省分离时间,而5mm皮条宽度太宽,不利于毛…     

儒家文化影响性文化

在中华文化发展史上,占最主要地位的是儒家文化。从历史来看,似乎儒家文化和性文化处于对立状态,但经作者分析研究认为,儒家文化与性文化有许多一致的、相互促进之处。儒家文化影响性文化。     

体外HPV感染皮肤粘膜细胞及器官模型的研究进展

  人乳头瘤病毒（HPV）感染与多种疾病相关,特别是高危型人HPV与生殖器肿瘤、皮肤肿瘤等发生发展存在关联。HPV阳性的肿瘤细胞系是较早研究HPV特性的体外细胞模型,特别是致癌特性。近年来,体外感染皮肤粘膜细胞系的出现推动HPV感染体外细胞模型的研究进程,但由于HPV的种属特异性以及复制和增殖对上皮细胞分化的依赖性,目前不能在常规的细胞培养中产生完整的感染过程。器官型培养模型的出现和发展可以模拟HPV病毒在皮肤粘膜的感染过程,是更好的研究HPV感染和治疗药物的模型。本文对目前体外HPV感染皮肤粘膜细胞及器官模型的研究进展进行综述。     

Quantitative digital image analysis applied to demonstrate the stratified distribution of involucrin in organ cultured human skin

Abstract In this study, quantitative digital image analysis was utilized to measure the optical density of immunostains of involucrin at different depths in the epidermis to obtain reliable ordinal-scaled interpretations of the staining intensity. The distribution of involucrin within the epidermis was investigated in air-liquid interface and submerged skin organ cultures at different time-points. A greyscale calibration procedure to standardize the optical units was used. By the 2nd day of culture, staining of involucrin had shifted markedly towards the mid or basal epidermis. Air-liquid interface cultures showed a less intensive shift than the submerged cultures. Up to the 7th day, involucrin staining remained in the upper epidermis in the air-liquid interface cultures, though weak staining was already observed in the basal epidermis. The results suggest that air-liquid interface conditions maintained physiological conditions better than submerged conditions which result in cultures that may have to increase their involucrin synthesis to improve the barrier function against the surrounding liquid during culture. Alternatively, changes in involucrin synthesis could reflect disturbed homeostasis. Concentrating measurements on certain cell layers might give more detailed information about changes in involucrin expression. Although the detection method was used to study the histochemistry of skin, it could easily be applied to other tissues as well. Received: 29 June 1998 / Received after revision: 28 October / Accepted: 2 November 1998     

采用HaCaT细胞培养沙眼衣原体

目的 探讨新的培养沙眼衣原体的细胞。方法 借鉴McCoy细胞培养沙眼衣原体的方法,将E型标准株和临床标本接种于HaCaT细胞,分别用碘染、单克隆荧光抗体检测、PCR扩增沙眼衣原体内源性质粒的方法检测沙眼衣原体是否可以在体外感染HaCaT细胞。用HaCaT细胞传代培养沙眼衣原体E型标准株5代,分别计数5次传代的包涵体数,并用SPSS17软件进行单因素方差分析,以确定沙眼衣原体在HaCaT细胞中是否增殖。结果 碘染后可见 HaCaT细胞胞质内有典型的包涵体。单克隆荧光抗体检测,在荧光显微镜下可见HaCaT细胞内有黄色荧光颗粒。PCR扩增HaCaT细胞内沙眼衣原体内源性质粒为阳性。沙眼衣原体E型标准株在HaCaT细胞内经传代5次,包涵体数逐渐增加。结论 HaCaT细胞在体外培养沙眼衣原体成功,且沙眼衣原体E型标准株在HaCaT细胞中可以增殖。     

不同DNA提取方法对PCR检测解脲支原体的影响

目的：比较PK、NK和PCI三种不同DNA提取方法对解脲支原体PCR的影响。方法：从临床得到的330份怀孕妇女的尿标本，接种到液体培养基和A8琼脂平板，液体培养阳性的标本1／10稀释后，用PK、NK和PCI三种不同DNA提取方法进行提取，分别作PCR及抑制实验，比较三种提取方法的敏感性和特异性。结果：液体培养和A8琼脂平板培养的阳性率分别为68．1％和39％，两者符合率为76％，PK、NK和PCI的敏感性为52．5％，55％，87．5％；特异性为65．8％，76．3％，60．5％；抑制率为33．3％，33．0％，3．3％。结论：在PK、NK和PCI三种不同DNA提取方法中，以PCI敏感性最高，抑制率最低。     

Ex vivo human skin and SZ95 sebocytes exhibit a homoeostatic interaction in a novel coculture contact model

The sebaceous gland displays key functions of the human skin, such as hormone synthesis in situ, antimicrobial activity and participation to inflammatory responses. Consequently, there is an emerging need of advanced in vitro models to study complex interactions between the sebaceous gland and the other skin compartments. Despite the evolution of both full‐skin organ culture and reconstructed three‐dimensional skin models, no satisfactory solutions have been provided for the integration of sebaceous glands and/or sebaceous gland cells in those models, probably due to their problematic maintenance both in vitro and ex vivo. We have developed a coculture model of explant skin in direct contact with immortalized SZ95 sebocytes, which resulted in overall improved structural integrity of the epidermis, higher percentage of proliferating basal epidermal cells and reduced apoptosis of differentiating keratinocytes after 6 days, as detected by Ki67 and TUNEL staining, respectively. Furthermore SZ95 sebocytes exhibited morphological and biochemical signs of normal differentiation and lipid accumulation, while interleukin‐6 expression in the supernatant of the cocultures was decreased in comparison with the control. The data provide evidence of a beneficial interaction between sebocytes and skin explants and provide the rationale for their integration in future three‐dimensional skin models.     

A three-dimensional skin culture model for mouse keratinocytes: application to transgenic mouse keratinocytes

The study of mouse epidermal biology has been hampered by the lack of a good in vitro model for the culture of mouse keratinocytes which allowed the reconstruction of a fully differentiated epidermis. We adapted the Pruniéras' model, also called the Dead de-Epidermized Dermis model (DED), to mouse keratinocytes and showed that a neo-epidermis can be reconstructed exhibiting a complete differentiation program. We also used this model to culture transgenic mouse keratinocytes. We observed that transgene expression occurred in the correct location and that the neo-epidermis mimed previous in vivo observations obtained with integrin skin-targeted transgenic mice. Therefore, this model will be a powerful tool to further investigate normal mouse and transgenic keratinocyte biology.     

Dispersed Cell Culture of Human Sweat Duct Cells under Serum-free Conditions

Human eccrine gland duct cells were successfully cultured using a serum-free medium, K-GM medium. Eccrine sweat ducts were isolated from dispase treated skin specimens from palms or soles. After treatment of the isolated ducts with trypsin and EDTA, dispersed cells were cultured in K-GM medium. In primary cultures, small colonies were seen 3 to 4 days after inoculation. Then the cells rapidly proliferated and formed large colonies with a paving stone-like cell arrangement. During the culture, small dome shaped areas were sometimes formed in the centers of colonies. Cultures multiplied for a maximum of 7 passages. The plating efficiencies of the 1st to 6th passage cells were about 20% to 30%. Immunocytochemically, cultured cells were positively stained with anti-carcinoembryonic antigens, K8.37 and K8.13, but not with anti-S100 protein, anti-HLA-DR, 34βB4, or PKK3. An electron micrograph of the cultured cells showed a multilayer of flattened cells linked by desmosomes. These results indicate that the cultured cells possessed the staining properties compatible with those of the ductal portion of eccrine sweat glands. No contamination by other mesenchymal cells, such as fibroblasts, was seen during the culture.     

裸鼠重建毛囊的组织学研究

目的 观察毛乳头细胞诱导毛囊再生民政部和毛囊重建情况。方法 采用器官型培养技术和裸鼠移植技术,将培养的毛囊毛乳头细胞、真皮鞘细胞和头皮（或包皮）真皮成纤维细胞分别制成胶原凝胶,再接种于毛囊上皮上段、下段和球部细胞,进行体外增习移植试验,结果 在毛囊毛乳头细胞与毛囊各段上皮细胞的器官型培养中均可见毛囊样结构形成;毛囊真皮鞘细胞凝胶上培养的毛球部细胞也重建出毛囊样结构。移植到裸鼠皮下后则可见较为完整的     

对部分大学生沉迷网络色情文化的心理分析

网络色情文化不仅对人们身心发展与性价值观影响甚大,甚至会使人们陷入色情风险情境当中。作为未来社会中流砥柱、社会生活方式引领者的大学生群体应远离网络色情文化,但现实却是部分大学生沉迷于网络色情文化,严重影响其工作和学习生活。为此本文从不良社会文化、大学生性意向发展的特点、部分大学生价值观发展的特点以及大学生需要的补偿等方面探讨了部分大学生沉迷网络色情文化的心理原因。