胰岛素样生长因子-1对成骨细胞生长影响的实验研究

目的:探讨胰岛素样生长因子-1对成骨细胞生长增殖的影响.方法:采用酶消化法获取兔骨膜成骨细胞,取第三代成骨细胞与0.1ng/ml、1ng/ml、10ng/ml的胰岛素样生长因子-1共同体外培养,在第1～7d观察细胞的形态、生长特点,MTT法测定细胞增殖情况,并通过细胞计数绘制细胞生长曲线.结果:不同浓度的胰岛素样生长因子-1对成骨细胞的生长增殖与对照组比较均有显著性差异(P＜0.01),各浓度之间对成骨细胞的生长增殖亦存在显著性差异(P＜0.05).结论:胰岛素样生长因子-1对成骨细胞生长增殖具有促进作用,在0.1～10ng/ml范围内此种作用与浓度呈正相关.     

胰岛素样生长因子-1(IGF-1)对成骨细胞的成骨影响

目的：探讨不同浓度IGF－1对兔成骨细胞的成骨影响。方法：采用组织块细胞培养技术获得兔成骨细胞，第2代成骨细胞分别在含0.1ng/ml,1ng/ml,10ng/ml,20ng/mlIGF－1的DMEM中培养，24，36h后行MTT法检测细胞增殖情况，72，108h收集培养上清进行骨钙素放射免疫法（RI）测定。结果：IGF－1与成骨细胞培养24，36h，经MTT法检测，不同浓度IGF－1与对照组比较，有显著性差异（P＜0．001）；IGF－1浓度为0．1、1、10ng/ml，各组之间相比存在显著性差异（P＜0．05）；IGF－1与成骨细胞培养72．108h，经RI检测，不同浓度IGF－1对成骨细胞合成骨钙素与对照组比较无显著性差异（P＞0．05）：结论：IGF－1对成骨细胞有明显促进增殖作用，在0．1－10ng/ml范围，存在浓度依赖性，未发现IGF－1对成骨细胞合成骨钙素有影响。     

重组人酸性成纤维细胞生长因子对人成骨细胞生长及增殖的影响

目的研究酸性成纤维细胞生长因子(acidfibroblastgrowthfactoraFGF)对成骨细胞生长及增殖的影响。方法取人胚胎颅盖骨骨内膜,利用组织块培养法进行成骨细胞原代培养,DMEM-F12培养液传代、扩增培养,以细胞形态学、细胞形成钙结节的能力及碱性磷酸酶活性鉴定成骨细胞。应用光镜、电镜、MTT法及流式细胞仪检测,分别从细胞形态、细胞生长及增殖特性分析不同浓度的aFGF对人成骨细胞生长和增殖的影响。结果10～100ng/mlaFGF可明显促进成骨细胞生长、增殖使S期细胞数增加,但高浓度aFGF(>1μg/ml)对成骨细胞生长具有抑制作用。结论aFGF对体外培养的人成骨细胞具有促生长和增殖作用,其有效浓度为10～100ng/ml。     

胰岛素样生长因子对体外培养豚鼠肋软骨细胞的影响

目的：了解胰岛素样生长因子(insulin-1ike growthfactor-1，IGF-1)对体外培养豚鼠肋软骨细胞分裂增殖及功能代谢的影响。方法：体外单层培养豚鼠肋软骨细胞，对照组培养液为无血流清DMEM，实验组在培养液DMEM中加入IGF-1使其终浓度分别为10ng／ml、50ng／ml、100ng／ml，作用6天后，检测细胞DNA含量和培养液中糖醛酸的含量。结果：IGF-1在10～100ng／ml浓度范围能明显增加培养软骨细胞的DNA及糖醛酸的含量，且以50ng／ml作用效果最明显，与对照组相比，有统计擘意义(P＜0．01)。结论：IGF-1对体外培养肋软骨细胞有刺激，并以剂量依赖性方式影响细胞的增殖及功能代谢。     

OGP和IGF-1对人牙周膜细胞增殖和总蛋白含量的影响

目的 探讨成骨生长肽(OGP)和胰岛素样生长因子-1(IGF-1)对体外培养的人牙周膜细胞(PDLC)增殖及其总蛋白含量的影响.方法 体外培养人PDLC与不同浓度的OGP和IGF-1单独或联合应用,用四甲基偶氮唑盐比色法(MTT)观察人PDLC增殖的情况,用考马斯亮蓝染色法检测细胞内总蛋白含量的变化.结果 OGP明显促进人PDLC增殖,第3天最大显效浓度为10-9mol/L,最小显效浓度为10-11mol/L.IGF-1也促进人PDLC增殖,第3天最大显效浓度为100ng/ml,最小显效浓度为1ng/ml.在第1、3、6天,OGP与IGF-1最大显效浓度联合和最小显效浓度联合,对人PDLC增殖活性和细胞内总蛋白合成的促进作用均较对照组增加.结论 OGP和IGF-1均可促进人PDLC增殖和细胞内总蛋白含量的提高,在一定范围内呈浓度时间依赖关系,二者联合具有协同效应.     

rhIGF-1对成骨细胞增殖、BMP-2及Cbfa1基因表达的影响

目的通过重组人胰岛素样生长因子(rhIGF-1)对体外成骨细胞增殖、骨形态蛋白-2(BMP-2)及核心结合因子1(Cbfa1)基因表达的影响,探讨rhIGF-1对骨代谢影响的可能机制。方法不同浓度的rhIGF-1(0、10、50、100ng/ml)刺激体外培养的大鼠成骨细胞,采用噻唑蓝(MTT)法检测细胞增殖能力,用半定量RT-PCR法检测成骨细胞BMP-2、Cbfa1基因的表达。结果rhIGF-1可明显促进成骨细胞增殖(P0.05),并促进成骨细胞BMP-2、Cbfa1基因的表达(P0.01),具有浓度依赖性。结论rhIGF-1可促进成骨细胞的增殖、分化及成熟,可能是通过增强BMP-2、Cbfa1基因的表达来实现的。     

胰岛素样生长因子-Ⅰ对兔关节软骨细胞增殖及代谢的影响

目的　探讨胰岛素样生长因子 - (IGF- )对体外生长的关节软骨细胞分裂增殖及功能代谢的影响。方法　采用体外单层培养的方法 ,应用兔关节软骨细胞 ,对照组培养液为 10 %小牛血清 DMEM,实验组在培养液DMEM中加入 IGF- 使其终浓度分别为 3、10、30、10 0及 30 0 ng/ ml,作用细胞 2、4和 6天 ,检测细胞 DNA含量和基质中糖醛酸的含量。结果　 IGF- 在 3～ 30 0 ng/ ml浓度范围能明显增加培养软骨细胞的 DNA及糖醛酸的含量 ,且以30～ 10 0 ng/ ml作用 4天刺激效果最明显 ,与对照组相比 ,有统计学意义 (P<0 .0 1)。结论　 IGF- 对体外培养软骨细胞有刺激 ,并以剂量时间依赖性方式影响增殖及功能代谢。     

骨组织工程中促细胞粘附因子bFGF的研究

目的：针对骨组织工程中成骨细胞与基质材料间促粘附这一重要问题,研究碱性成纤维细胞生长因子（bFGF)对成骨细胞粘附特性的影响。方法：取兔骨髓基质干细胞来源的成骨细胞体外培养,分别用0ng/ml、10ng/ml、100ng/ml浓度的bFGF诱导培养,观察接种后各时间点成骨细胞粘附情况,体视学计量粘附细胞数量。结果：10ng/mlbFGF诱导成骨细胞24小时后,细胞粘附数量增加最明显,显高于对照组及100ng/ml组（P＜0．01）。实验还发现,成骨细胞粘附最快发生在接种后4小时内。结论：一定浓度碱性成纤维细胞生长因子具有促进成骨细胞粘附特性的作用。     

重组人胰岛素样生长因子Ⅰ对大鼠成骨细胞增殖、凋亡及Ⅰ型胶原蛋白合成的影响

目的 观察胰岛素样生长因子Ⅰ（IGF-Ⅰ）对体外培养的大鼠成骨细胞的增殖、凋亡及Ⅰ型胶原蛋白合成的影响，探讨IGF-Ⅰ对骨代谢影响的可能机制。方法 不同浓度rhIGF-Ⅰ刺激体外培养的大鼠成骨细胞，采用噻唑蓝（MTT）法测定细胞增殖能力；肿瘤坏死因子α（TNF-α单独或与rhIGF-Ⅰ共同刺激成骨细胞，流式细胞仪检测细胞周期和凋亡率；rhIGF-Ⅰ刺激成骨细胞，免疫细胞化学结合计算机图像分析系统检测Ⅰ型胶原蛋白的表达。结果 一定浓度IGF-Ⅰ能明显增加成骨细胞数量（P〈0．05），在0．1～100ng／ml这种作用与IGF-Ⅰ的浓度呈正相关；TNF-α在0．1～100ng/ml浓度范围内呈剂量依赖性地促进成骨细胞凋亡（P〈0．05），并使S期细胞减少（P〈0．05），而IGF-Ⅰ能抑制，TNF-α对成骨细胞的促凋亡作用（P〈0．05）；经IGF-Ⅰ的刺激，成骨细胞Ⅰ型胶原蛋白的表达明显高于对照组（P〈0．05）。结论 IGF-Ⅰ对大鼠成骨细胞有明显的促增殖作用，在0．1～100ng/ml之间呈浓度依赖性，IGF-Ⅰ能抑制，TNF-α诱导的大鼠成骨细胞凋亡，IGF-Ⅰ能促进大鼠成骨细胞Ⅰ型胶原蛋白的合成。     

HB-EGF对系膜细胞增殖及转化生长因子-β1表达的影响

目的：观察肝素结合性表皮生长因子样生长因子（HB-EGF）对肾小球系膜细胞（GMC）增殖及转化生长因子-β1（TGF-β1）表达的作用。方法：以原代培养大鼠肾小球系膜细胞为细胞模型,采用MTT、ELISA、RT-PCR等方法观察外源性HB-EGF对系膜细胞增殖及TGF-β1表达的影响。结果：（1）不同浓度HB-EGF对系膜细胞增殖有不同影响,10ng/ml时无刺激GMC增殖作用（P〉0.05）,100、1000ng/ml均能刺激GMC增殖（P〈0.01）,100ng/ml作用最明显。（2）HB-EGF（10ng/ml、100ng/ml、1000ng/ml）能刺激系膜细胞TGF-β1的表达（P〈0.01）,100ng/ml时作用最强。（3）100ng/mlHB-EGF刺激GMC24h、36h、48h,系膜细胞TGF-β1的表达均增加,随作用时间而变化,刺激36h和48h比24h作用更明显（P〈0.05）。结论：一定浓度范围内HB-EGF刺激GMC增殖,促进系膜细胞TGF-β1的表达,且具有一定的时间及浓度依赖性。     

Scoliosis and Growth: Patterns of Asymmetry in Normal Vertebral Growth

This study reviews published observations of asymmetrical appearance of primary ossification centres in human fetal vertebral arches. It reports studies of vertebral asymmetry in 39 vertebral columns of infants and children including asymmetry in pedicle length and vertebral arch height, asymmetry in neurocentral fusion, and vertebral body flattening on its left anterior aspect. It relates these patterns of asymmetry to the commonly observed left thoracic scoliosis of infancy and right thoracic scoliosis of adolescents and adults. It discusses the implications of these observed asymmetries of normal vertebral growth in the aetiology of scoliosis, and the possible influences of handedness and aortic pressure in the production of these vertebral asymmetries in adolescence.     

Scoliosis and Growth: Patterns of Asymmetry in Normal Vertebral Growth

This study reviews published observations of asymmetrical appearance of primary ossification centres in human fetal vertebral arches. It reports studies of vertebral asymmetry in 39 vertebral columns of infants and children including asymmetry in pedicle length and vertebral arch height, asymmetry in neurocentral fusion, and vertebral body flattening on its left anterior aspect. It relates these patterns of asymmetry to the commonly observed left thoracic scoliosis of infancy and right thoracic scoliosis of adolescents and adults. It discusses the implications of these observed asymmetries of normal vertebral growth in the aetiology of scoliosis, and the possible influences of handedness and aortic pressure in the production of these vertebral asymmetries in adolescence.     

Role of Insulin-like Growth Factor-1 in the Regulation of Skeletal Growth

The importance of the insulin-like growth factor (IGF)-I axis in the regulation of bone size and bone mineral density, two important determinants of bone strength, has been well established from clinical studies involving patients with growth hormone deficiency and IGF-I gene disruption. Data from transgenic animal studies involving disruption and overexpression of components of the IGF-I axis also provide support for a key role for IGF-I in bone metabolism. IGF-I actions in bone are subject to regulation by systemic hormones, local growth factors, as well as mechanical stress. In this review we describe findings from various genetic mouse models that pertain to the role of endocrine and local sources of IGF-I in the regulation of skeletal growth.     

Growth of Incidental Meningiomas

The aim of this study was to assess the growth of incidental meningiomas, to establish a strategy for dealing with these tumours. The cases of 37 patients with a meningioma revealed incidentally by computerized tomography or magnetic resonance imaging, who were followed at least once by an additional imaging study, were reviewed. The tumour volume was calculated, to estimate the annual growth rate of the incidental meningiomas. Nine of the 37 patients (24.3%) showed a considerable increase (the annual growth rate > 1 cu cm/year) in their tumour volume (tumour growth). There was no significant difference in the follow-up period, age, or the volume of tumour between the patients with and without tumour growth. However, a multivariate analysis revealed that the likelihood of tumour growth independently and significantly increased according to a decrease in the age of the patients (Odds ratio 0.18 for one-standard-deviation change (ISD) 12.6 years, p = 0.042) and according to an increase in the volume of the tumour (Odds ratio 3.64 for ISD 4.46 cu cm, p = 0.042). The majority of patients with incidental meningioma can be apparently observed without any surgical intervention, because their annual growth rates are generally less than 1 cu cm/year. However, clinical and radiological observations would be advisable for these patients (especially young patients and patients with a large tumour), in view of the presence of rapidly growing tumours in some of the patients.     

Effect of Growth Factors on Growth of Bovine Parathyroid Cells in Serum-Free Medium

p < 0.05) and IGF-I and IGF-II alone ( p < 0.05) significantly stimulated growth over controls ( t -test). Furthermore, the combination of TGFα with IGF-I and IGF-II exhibited significant enhancement above that seen with IGF-I and IGF-II alone ( p < 0.01). EGF did not stimulate growth over controls. EGFr may be expressed in BPCs, but TGFα exhibits a more potent growth stimulus than EGF. Addition of IGF-I or IGF-II to the growth medium further enhances this effect.