高压氧联合复方丹参注射液治疗新生儿缺氧缺血性脑病疗效分析

目的：评价高压氧联合复方丹参注射液对新生儿缺氧缺血性脑病的疗效。方法：将60例患儿随机分为高压氧组、高压氧联合复方丹参组及对照组。对照组仅进行常规治疗，高压氧组在常规治疗同时进行高压氧治疗，每日一次，10天为一疗程，高压氧联合复方丹参组在高压氧组治疗基础上加点复方丹参注射液，10天为一疗程。结果：高压氧联合复方丹参组有效率明显高于对照组，也高于单用高压氧组，疗效有显著差别。结论：高压氧联合复方丹参注射液对新生儿缺氧缺血性脑病疗效稳定，无毒副作用，值得推广。     

纳洛酮和复方丹参注射液联合治疗新生儿缺氧缺血性脑病临床观察

目的：探讨纳洛酮和复方丹参注射液联合治疗新生儿缺氧缺血性脑病（HIE）的临床疗效。方法：把62例HIE患儿随机分为治疗组32例和对照组30例。对照组常规治疗,治疗组在常规治疗基础上加用纳洛酮和复方丹参注射液。通过观察临床症状恢复时间及新生儿神经行为（NBNA）评分来判定药物的疗效。结果：治疗组疗效明显优于对照组。结论：纳洛酮和复方丹参注射液联用有缩短HIE病程的作用,对提高治愈率、降低病残率有积极的意义。     

复方丹参注射液氧驱动雾化吸入辅助治疗高原地区毛细支气管炎患儿的临床疗效观察

目的：观察氧驱动复方丹参注射液雾化吸入辅助治疗高原地区毛细支气管炎患儿的临床疗效。方法：将86例毛细支气管炎患儿随机分为复方丹参注射液治疗组和对照组,治疗组患儿在常规治疗的基础上雾化吸入复方丹参注射液;对照组患儿在常规治疗基础上雾化吸入α-糜蛋白酶,两组均为2次/天,疗程（3～5）天。比较两组在咳嗽消失、气喘缓解、哮鸣音消失及住院时间方面的差异。结果：治疗组在缓解气喘、咳嗽消失、肺部哮鸣音消失、缩短住院时间方面明显优于对照组,差异有显著性（P〈0.05）。结论：复方丹参注射氧动液雾化吸入是临床辅助治疗高原毛细支气管炎患儿安全、有效的药物。     

胞二磷胆碱与复方丹参注射液治疗新生儿缺氧缺血性脑病35例临床观察

目的:观察胞二磷胆碱与复方丹参注射液治疗新生儿缺氧缺血性脑病的临床效果。方法:选择在我院住院的缺氧缺血性脑病新生儿患儿共70例,随机分为观察组和对照组各35例,两组患儿均给予供氧、控制惊厥、治疗脑水肿、限制入液量和对症支持等常规治疗。对照组给予单一注射神经生长因子;观察组给予胞二磷胆碱与复方丹参注射液治疗。结果:观察组总有效率明显优于对照组,差异有统计学意义(P<0.05)。结论:常规治疗基础上加用胞二磷胆碱与复方丹参注射液治疗新生儿缺氧缺血性脑病疗效满意,值得推广。     

复方丹参注射液加高压氧治疗新生儿缺氧缺血性脑病及预后研究

目的：探讨应用复方丹参注射液加高压氧治疗新生儿缺氧缺血性脑病的临床效果及远期预后情况方法：采用随机分组分为治疗组和对照组，两组均给常规治疗，治疗组加用复方丹参注射液、高压氧治疗，观察近期疗效、行为神经评分及长期随访。结果：治疗组近期疗效及长期随访结果均明显优于对照组．结论：复方丹参注射液加高压氧治疗新生儿缺氧缺血性脑病能起到积极的治疗作用并能明显改善预后     

复方丹参注射液早期治疗新生儿中重度缺氧缺血性脑病的疗效分析

目的：观察复方丹参注射液对中重度缺氧缺血性脑病（HIE）的治疗效果。方法：86例中重度HIE新生儿随机分为两组：常规治疗组（对照组）42例,即三支持、两对症;复方丹参注射液干预组44例,在常规治疗的基础上早期（生后6h内）应用复方丹参注射液治疗。比较两组患儿头颅CT变化、行为神经评分（NBNA）及12个月时CDCC测定结果。结果：复方丹参注射液干预组头颅CT改变、NBNA评分及MDA、PDA等各项指标与对照组比较均有明显改善（P〈0.05）。结论：早期应用治疗复方丹参注射液中重度HIE有明显疗效。     

复方丹参注射液治疗新生儿缺氧缺血性脑病

６０例新生我缺氧缺血性脑病患儿随机分为复方丹参注射液治疗组和常规治疗对照组各３０例。两组患儿在一般情况,孕产期情况,临床表现,影像学检查方面均相似（Ｐ〉０．０５）。治疗组在常规治疗基础上给复方丹参４ml加１００g/Ｌ葡萄糖２０ml,一日１次。结果显示复方丹参治疗组临床疗效明显优于对照组（Ｐ〈０．０５）,说明复方丹参注射液能减轻新生儿缺氧缺血性脑病的脑损伤。     

联用纳络酮及复方丹参注射液治疗HIE疗效观察

目的：观察纳络酮及复方丹参注射液联用治疗新生儿缺氧缺血性脑病（ＨＩＥ）临床效果。方法：对６９例中、重度ＨＩＥ患儿随机分为治疗组和对照组,对照组给予常规治疗,治疗组在常规治疗基础上联用纳络酮及复方丹参注射液。结果：治疗组总有效率 ９４．５９％,对照组总有效 ６２．５０％,Ｘ２＝ ９．５２,Ｐ＜０．０１,差别有非常显著性。结论：纳络酮及复方丹参注射液治疗ＨＩＥ有显著疗效,明显提高治愈率。     

复方丹参注射液治疗急性高血压性脑出血的临床观察

目的：观察复方丹参注射液治疗急性高血压性脑出血的疗效。方法：60例急性高血压性脑出血随机分为复方丹参组30例，对照组（西药常规治疗）30例。复方丹参治疗组在西药常规治疗基础上加用复方丹参注射液16ml加入5％葡萄糖500ml静滴，1次／d，连续14天，观察28天，评定疗效。结果：复方丹参组总有效率为96．67％，优于对照组73．33％（P＜0．05）；复方丹参组治疗14天CT复查血肿平均吸收率为62．96％，优于对照组44．96％，优于对照组44．96％。结论：复方丹参注射液治疗急性高血压性脑出血有较好疗效，安全，值得临床应用。     

干扰素丹参佐治毛细支气管炎观察

目的：探讨临床应用干扰素、复方丹参注射液超声雾化吸入治疗婴幼儿毛细支气管炎的疗效。方法：60例患儿随机分为干扰素，复方丹参注射液及超声雾化吸入的治疗组30例和常规治疗对照组30例。结果：采用干扰素、复方丹参注射液超声雾化治疗毛细支气管炎较对照组有明显的差别。结论：应用干扰素复方丹参注射液联合超声雾化吸入治疗婴幼儿毛细支气管炎疗效显著。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.