艾灸对大鼠皮肤创伤成纤维细胞和KGF表达的影响

目的：研究艾灸对大鼠创伤皮肤组织成纤维细胞和角化细胞生长因子（KGF）表达的影响,探讨艾灸对大鼠皮肤创伤修复的作用。方法：建立大鼠背部皮肤创伤模型,采用随机数字表法,将24只大鼠分为治疗组和模型组。根据治疗时间的不同,又将每组分为1天、3天、7天共3个时间点,每个时间点4只大鼠。治疗组进行艾灸治疗,每次30 min,每日1次。以HE染色法检测1天、3天、7天3个时间点修复组织内成纤维细胞数;以免疫组化法检测1天、3天、7天等3个时间点修复组织成纤维细胞生长因子的表达情况。结果：大鼠创面愈合指数治疗组高于模型组（P〈0.05）。大鼠皮肤创面成纤维细胞数,7天治疗组皮肤创面成纤维细胞数高于模型组（P〈0.05）;3天两组比较差异无统计学意义（P〉0.05）。大鼠创面KGF表达情况,3天模型组KGF表达呈弱阳性,治疗组表达呈阳性;7天模型组KGF表达呈阳性,治疗组表达呈强阳性。结论：艾灸能促进大鼠皮肤创伤愈合,其机制可能与调节不同时间内创面组织成纤维细胞数和KGF含量有关。     

艾灸对大鼠创伤皮肤组织血管内皮细胞和血管内皮生长因子表达的影响

目的:探讨艾灸干预大鼠皮肤创伤愈合的作用及作用机制。方法:将24只SD大鼠在其背部制造全层皮肤切线性创伤,以随机数字表法,随机分成治疗组和模型组,每组12只。再根据治疗时间的不同将每组随机分为1d组、3d组、7d组3个时间点小组,每个时间点小组4只大鼠。治疗组取创面局部进行艾灸治疗,每次30min,每日1次。以HE染色法检测修复组织内毛细血管生长情况、血管内皮细胞数;以免疫组化法检测修复组织内血管内皮细胞生长因子的表达情况。结果:大鼠创面愈合指数,治疗7d组在治疗第4天与第8天时均高于模型7d组(均P0.05)。大鼠创面修复组织毛细血管数,治疗1d组和3d组均高于相应模型组(均P0.05);治疗7d组毛细血管数低于模型7d组(P0.05)。大鼠创面修复组织血管内皮细胞数,治疗3d组高于模型3d组(P0.05);治疗7d组低于模型7d组(P0.05);治疗1d组与模型1d组比较差异无统计学意义(P0.05)。大鼠创面修复组织VEGF表达阳性细胞累计积分,治疗3d组高于模型3d组(P0.05);治疗7d组低于模型7d组(P0.05);治疗1d组与模型1d组比较差异无统计学意义(P0.05)。结论:艾灸能促进大鼠皮肤创伤愈合,其机制可能与调节不同时间内创面组织中血管内皮细胞和血管内皮生长因子含量有关。     

玉糊浆促进大鼠背部创面愈合的实验研究

目的 观察玉糊浆对大鼠背部创面愈合速度的影响,探讨其促进愈合的机制。方法 大鼠造模后根据随机数字表法将其分为玉糊浆组、凡士林组及模型组,每组 18 只。记录 3、7、14 天后创面愈合面积、HE 染色观察组织形态以及免疫组化测定肉芽组织中血管内皮生长因子（VEGF）、肿瘤坏死因子-α（TNF-α）、IL-6、IL-8、糖原合成激酶 3β（ GSK-3β）、β-连环蛋白（β-catenin）的表达。结果 创伤后第 3、7、14 天,与模型组比较,凡士林组创面面积差异无统计学意义（P>0.05）,玉糊浆组创面面积缩小（P<0.05）;与凡士林组比较,玉糊浆组创面面积缩小（P<0.05）;病理观察发现院创伤后第 3、7、14 天,与模型组比较,凡士林组创面坏死组织、炎症细胞、肉芽组织中毛细血管及纤维细胞差异不明显,玉糊浆组创面坏死组织及炎症细胞少,肉芽组织中毛细血管及纤维细胞增生明显;与凡士林组比较,玉糊浆组创面坏死组织、炎症细胞更少,肉芽组织中毛细血管及纤维细胞增生明显;创面组织各检测指标情况院与模型组同期比较,凡士林组第 3 天各检测指标水平差异无统计学意义（P>0.05）,第 7 天 GSK-3β 降低（P<0.05）,第 14 天 VEGF 升高（P<0.05）、IL-8 降低（P<0.05）;玉糊浆组第 3、7、14 天 β-catenin、VEGF 升高（P<0.05）、IL-8 降低（P<0.05）,第 7、14 天 GSK-3β 降低（P<0.05）,第 7 天 IL-6 降低（P<0.05）,第 14 天 TNF-α 降低（P<0.05）;与凡士林组同期比较,玉糊浆组第 3、7、14 天 β-catenin、VEGF 升高（P<0.05）、IL-8 降低（P <0.05）,第 3、14 天GSK-3β 降低（P<0.05）,第 7、14 天 TNF-α 降低（P<0.05）,第 7 天 IL-6 降低（P<0.05）。结论 玉糊浆可能是通过调控 Wnt/β-catenin 信号通路中 β-catenin 表达上调、GSK-3β 表达下调,以及提高创面肉芽组织中 VEGF 浓度,降低 IL-6、IL-8 及 TNF-α 浓度,达到促进创面愈合的目的。     

艾灸对腹膜透析大鼠腹膜组织形态学及VEGF、NO的影响

目的观察艾灸对腹透模型大鼠腹膜结构、血管内皮生长因子（Vascular Endothelial Growth Factor,VEGF）及一氧化氮（Nitrogen Monoxide,NO）的影响。方法将大鼠随机分为正常组、模型组、假透组和艾灸治疗组,采用腹腔注射高糖腹透液（葡萄糖浓度为2.5%）制备大鼠腹透模型。艾灸治疗组以腹腔注射前30 min温和灸神阙穴5 min为治疗方案。应用HE染色观察大鼠腹膜组织结构变化,硝酸还原酶法测定血清NO含量,采用酶联免疫吸附法和Western Blotting测定血清与腹膜组织中VEGF含量。结果 HE染色结果显示模型组大鼠腹膜组织结构欠完整,血管分布不均匀,数量减少,伴管壁明显异常增厚,与模型组比较,艾灸治疗组大鼠腹膜组织结构有明显改善,表现为大鼠腹膜组织完整,血管数量增加,管壁仅见轻度增生。与正常组比较,模型组大鼠血清与腹膜组织中VEGF含量降低,血清中NO含量增加（P〈0.05）;与模型组比较,艾灸治疗组血清中VEGF含量增加、NO含量降低（均P〈0.01）,腹膜组织内VEGF表达有增加趋势但无统计学意义。结论艾灸神阙穴能一定程度上降低腹透液引起的腹膜组织结构损伤,增加血清VEGF含量,降低血清NO含量。     

艾灸及艾烟对APOE基因敲除小鼠血清促炎因子的干预研究

目的:观察艾灸及艾烟对APOE-/-小鼠血清细胞间黏附分子(ICAM1)、血管细胞黏附分子(VCAM1)以及单核细胞趋化因子(MCP-1)水平的影响,以期初步探讨艾灸防治动脉粥样硬化的机制。方法:采用高脂饮食喂养的载脂蛋白E基因敲除小鼠(APOE-/-小鼠)作为动脉粥样硬化模型,并随机分为4组:艾灸组、艾烟组、香烟对照组组、模型组。分别运用艾灸、艾烟及香烟的方式进行干预,干预12周后观察小鼠血清ICAM1、VCAM1及MCP-1水平的变化。结果:与模型组相比,艾灸组,艾烟组小鼠血清ICAM1、VCAM1及MCP-1水平明显降低(P0.05),艾灸组,艾烟组之间无统计学意义。香烟组于模型组之间差异无统计学意义。结论:艾灸及艾烟可明显降低动脉粥样硬化小鼠血清中黏附分子和单核趋化分子的水平。     

抗炎——艾灸温通的效应机制

从艾灸适应病谱和古代文献记载分析艾灸抗炎的普遍性;从艾灸治疗炎性疾病的系列研究、热证可(贵)灸的科学内涵指出艾灸抗炎的特异性;从艾灸对慢性炎性反应的4个基本通路均可以产生影响的系列研究结果说明艾灸抗炎的整体性;从对炎性反应的过度与不足均具有调整作用说明艾灸抗炎的双向性,论证艾灸温通效应机制主要在于其抗炎作用.最后提出灸法抗炎与温热觉感受TRPV(瞬时受体电位香草酸亚家族)通道蛋白相关的可能性机制.艾灸温通效应机制在于抗炎,而艾灸抗炎机制的明确将为艾灸防治重大疾病提供理论基础.     

艾灸对佐剂性关节炎大鼠踝关节滑膜组织低氧诱导因子-1α和血管内皮生长因子表达的影响

目的:观察艾灸对佐剂性关节炎(AA)大鼠踝关节滑膜组织低氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)蛋白表达的影响,探讨艾灸抑制类风湿关节炎关节滑膜血管新生、改善关节症状的作用机制。方法:60只健康成年雄性SD大鼠随机分为正常组、模型组、艾灸组和甲氨蝶呤组,每组15只。采用右后足跖皮下注射弗氏完全佐剂法制备AA大鼠模型。艾灸组予双侧“足三里”“关元”和“阿是穴”艾灸治疗,每次20 min,每日1次,连续干预3周;甲氨蝶呤组予甲氨蝶呤(0.35 mg/kg)灌胃给药,每周2次,连续干预3周。采用足趾容积测量仪检测大鼠足跖容积;HE染色法观察大鼠踝关节滑膜组织病理情况;免疫组织化学法和Western blot法检测大鼠踝关节滑膜组织HIF-1α、VEGF蛋白表达情况。结果:与正常组比较,模型组大鼠的足趾容积和踝关节滑膜组织HIF-1α、VEGF蛋白表达均明显升高(P<0.01),滑膜组织出现明显增生且有大量新生血管形成;与模型组比较,艾灸组和甲氨蝶呤组大鼠的足趾容积和踝关节滑膜组织HIF-1α、VEGF蛋白表达均明显降低(P<0.05,P<0.01),滑...     

大鼠肾上腺摘除对艾灸抗炎免疫作用的影响

为探讨艾灸抗炎免疫作用机理，本实验观察了ＡＡ大鼠肾上朱摘除对灸疗作用的影响。结果表明，在肾上腺完整的情况下，艾灸能抗炎消肿，增强免疫功能，其跖转减小、胸脾指数改善、ＩＬ－６、ＩＬ－２含量趋于正常，与对照组比较差异显著（Ｐ〈０．０１）。肾上腺摘除后，灸疗的抗炎免疫作用被部分阻断或削弱，上述指标与肾上腺摘除对照组比较差异不显著（Ｐ〉０．０５）。提示灸疗可能通过激活ＨＰＡ系统对皮质激素的调控参与并影响其     

艾灸联合子午流注对大鼠背部创面修复作用的实验研究

目的:观察艾灸联合子午流注对大鼠背部创面愈合的促进作用并探讨其作用机制。方法:将90只SD大鼠随机分成模型组、普通艾灸组、子午流注组,雌雄各半,麻醉及消毒后按照造模标准将大鼠背部制成直径1.8 cm的创面,深达皮肤筋膜层,伤后第1天开始进行各项干预治疗,观察创面愈合的时间,计算创面愈合率,以HE染色光镜下观察巨噬细胞,中性粒细胞及微血管含量等;并通过免疫组化,运用图像分析等技术观察血管内皮生长因子(Vascular endothelial growth factor,VEGF)阳性表达细胞数。结果:与模型组比较,普通艾灸组和子午流注组创面愈合率、VEGF阳性表达均明显增加(P 0.05);与普通艾灸组比较,子午流注组创面愈合率、VEGF阳性表达均明显增加(P 0.05)。结论:艾灸联合子午流注能明显促进大鼠背部创面愈合,其机制可能跟其促进组织修复细胞以及炎性细胞的增殖,影响肉芽组织中VEGF的表达有关。     

265 六种印度植物抗炎和促伤口愈合作用的研究

本文报道了印度学者近期对六种当地产植物抗炎和促伤口愈合作用的研究结果。     

艾灸促进皮肤外伤愈合及对局部组织的修复作用

目的:探讨艾灸促进创面愈合疗效及其对创伤不同阶段局部组织形态的修复作用。方法:28只成年雄性SD大鼠随机分为模型组和艾灸组,每组14只。采用直径8mm的取皮器从大鼠背部正中线肩胛下角2cm处钻取全层皮肤制备大鼠外伤模型。造模后即刻予艾灸干预,每日1次,每次25min,持续6d。观察并拍照记录创面的愈合情况,造模后第2天和第7天采用HE和Masson染色观察各组大鼠创伤局部组织形态学变化和胶原纤维生长情况。结果:与模型组比较,艾灸组干预2次后大鼠创口面积就显著减小(P0.01,P0.05),随着干预次数增加艾灸组愈合面积显著增加(P0.01),艾灸组大鼠创伤局部组织中炎性细胞数量显著增多(P0.01),新生胶原纤维显著增多(P0.05);艾灸6次后,艾灸组较模型组创伤局部组织成纤维细胞数量和胶原纤维量明显增加,细胞和胶原纤维排列更加紧密,新生血管数量较多,受损部位的皮肤结痂较厚。结论:艾灸可以明显促进创伤局部胶原纤维生长和细胞增殖,促进外伤模型大鼠皮肤创面愈合。     

Influence of Terminalia chebula on dermal wound healing in rats

The effects of topical administration of an alcohol extract of the leaves of an evergreen plant, Terminalia chebula, on the healing of rat dermal wounds, in vivo, was assessed. T. chebula treated wounds healed much faster as indicated by improved rates of contraction and a decreased period of epithelialization. Biochemical studies revealed a significant increase in total protein, DNA and collagen contents in the granulation tissues of treated wounds. The levels of hexosamine and uronic acid in these tissues, also increased upto day 8 post-wounding. Reduced lipid peroxide levels in treated wounds, as well as ESR measurement of antioxidant activity by DPPH radical quenching, suggested that T. chebula possessed antioxidant activities. The tensile strength of tissues from extract-treated incision wounds increased by about 40%. In addition, T. chebula possessed antimicrobial activity and was active largely against Staphylococcus aureus and Klebsiella. These results strongly document the beneficial effects of T. chebula in the acceleration of the healing process.     

The effects of Polygonum cuspidatum extract on wound healing in rats

Aim of the study

Polygonum cuspidatum has long been used as a traditional medicine inducing wound healing. In this study, the extract from the Chinese medicinal herb Polygonum cuspidatum was investigated on its wound healing activity, in order to obtain an accurate elucidation of its traditional use value.

Materials and methods

After creating wound healing model on the back of rats, the extract from the Chinese medicinal herb Polygonum cuspidatum was applied. Wound healing rates were calculated at 3, 7, 14, and 21 days after the wounding, and tissues were harvested at 1, 3, 7, 14 and 21 days for histological and immunohistochemistry analysis. The stages of wound granulation tissues were evaluated histopathologically. The expression of TGF-β1 was determined by immunohistochemically.

Results

Wound healing rates were significantly higher at 3, 7, 14 and 21 days in the extract group than in the control (p < 0.05). Histological results showed more well-organized bands of collagen, more fibroblasts and hair follicle and less inflammatory cells in the extract group. The immunohistochemical results revealed that TGF-β1 increased in the extract group on day 1, 3 and 7 post-wounding (p < 0.05).

Conclusion

The present study has shown that the extract from the Chinese medicinal herb Polygonum cuspidatum possesses wound healing activity, and thus provided the evidence for its traditional use value.     

中药红油膏对大鼠创面愈合影响的实验研究

目的探讨红油膏促进大鼠创面愈合的作用机制。方法实验大鼠经手术造成背部创面,随机分为2组,实验组用红油膏涂布创面,对照组采用龙珠软膏。观察大鼠创面愈合时间;动物造模后第3,5,7天,创面取分泌物检测巨噬细胞以及创面肉芽组织测定蛋白质含量;造模后第1,3,5,7天,测定血清C反应蛋白(CRP)含量。结果与对照组比较,实验组能缩短大鼠创面愈合时间;实验组在相应时间内的巨噬细胞数量高于或显著高于对照组(P0.05或0.01);经红油膏治疗的大鼠皮肤创面,其组织中蛋白含量高于对照组(P0.05或0.01);术后第1天,实验组与对照组CRP均升高,之后实验组CRP下降,较对照组有显著性差异(P0.05或0.01)。结论红油膏能有效促进大鼠创面愈合。     

Influence of aloe vera on the healing of dermal wounds in diabetic rats

The positive influence of Aloe vera, a tropical cactus, on the healing of full-thickness wounds in diabetic rats is reported. Full-thickness excision/incision wounds were created on the back of rats, and treated either by topical application on the wound surface or by oral administration of the Aloe vera gel to the rat. Wound granulation tissues were removed on various days and the collagen, hexosamine, total protein and DNA contents were determined, in addition to the rates of wound contraction and period of epithelialization. Measurements of tensile strength were made on treated/untreated incision wounds. The results indicated that Aloe vera treatment of wounds in diabetic rats may enhance the process of wound healing by influencing phases such as inflammation, fibroplasia, collagen synthesis and maturation, and wound contraction. These effects may be due to the reported hypoglycemic effects of the aloe gel.     

Healing potential of Rosmarinus officinalis L. on full-thickness excision cutaneous wounds in alloxan-induced-diabetic BALB/c mice

Aim of the study

Rosmarinus officinalis (Rosemary) used in Jordanian folk medicine for wound management and treatment. Therefore, the present study was conducted to assess the healing efficacy of both aqueous extract and essential oil of the aerial parts on alloxan-induced diabetic BALB/c mice.

Materials and methods

Two full-thickness round wounds were created in the dorsal area of each mouse. Animals were divided into four groups of twenty mice each: untreated normal, untreated diabetic, aqueous extract- (intraperitoneal injection of 0.2 ml at a dose of 10% for 3 days) and essential oil-treated diabetic mice (topical application of 25 μl/excision wound, twice a day for 3 days). For 15 days, the wounds were visually observed; blood glucose level, body weight, regenerated granulation tissue weight and the percentage of wound contraction were measured. On days 6 and 15 after wounding, the animals were sacrificed and the histology of wound area was examined.

Results

Significant positive differences (p < 0.01) between treated and control groups were observed at different aspects of diabetic wound healing process. Reduced inflammation and enhanced wound contraction, re-epithelialization, regeneration of granulation tissue, angiogenesis and collagen deposition were detected in the treated wounds.

Conclusions

Results indicated that the essential oil of Rosmarinus officinalis was the most active in healing diabetic wounds and provide a scientific evidence for the traditional use of this herb in wound treatment. However, further scientific verification is required to confirm and assess the range of wound healing potential of essential oils of Rosemary chemotypes.     

芦荟在创伤愈合中的应用

对近几年芦荟在创面愈合中的应用进行归纳总结。芦荟具有抑菌、消炎、免疫调节、促肉芽组织形成、保湿、镇痛等药理作用,可用于抗菌、消肿生肌、修复组织损伤、促进创面愈合等;并对以芦荟为主体的一氧化氮缓释剂在创伤愈合中作用前景进行展望。     

Evaluation of phytoconstituents of Terminalia arjuna for wound healing activity in rats

The effect of topical application of phytoconstituents (fraction I, II and III) fractionated from a hydroalcohol extract of the bark of the plant, Terminalia arjuna, was assessed on the healing of rat dermal wounds using in vivo models. The results indicated a statistically significant increase in the tensile strength of the incision wounds and the percent epithelialization of excision wounds compared with control (p < 0.05). However, topical treatment with fraction I, consisting mainly of tannins, was found to demonstrate a maximum increase in the tensile strength of incision wounds. Even with respect to excision wounds, the fastest rate of epithelialization was seen with fraction I. Hexosamine estimation of granulation tissue obtained from excision wounds revealed an increase in the hexosamine content with fraction I compared with the control. In addition, fraction I from the hydroalcohol extract of Arjuna bark possessed antimicrobial activity against tested microorganisms such as Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes but not Candida albicans. These results strongly document the beneficial effects of fraction I, consisting mainly of tannins, of Terminalia arjuna in the acceleration of the healing process. Thus, the present study validates the claim made with respect to the plant as well as corroborating the astringent effect of tannins by drawing the tissues closer together.     

Topical continuous use of Lippia sidoides Cham. essential oil induces cutaneous inflammatory response,but does not delay wound healing process

Ethnopharmacological relevance

The essential oil of Lippia sidoides (EOLS) has been used in Brazilian folk medicine as a topical antiseptic agent in skin for treatment of wounds and superficial infections of the body. The aim of this study was to investigate the effects of EOLS on intact and damaged skin, including its action on expression of mediators, COX-2 and VEGF, involved in healing full-thickness cutaneous lesions in vivo.

Material and methods

EOLS was analyzed chemically and used at different concentrations to dose-response experiments in skin mice. Skin irritation tests by one-dosage and multiple-dosages and irritation to damaged skin were assessed by macroscopy, morphometry and histological and immunohistochemical analyses. To evaluate the effects of EOLS on wound healing, excision wounds were surgically created on the dorsum of rats, and the ointments at 6% and 12% were applied daily to the wound area. Cutaneous lesions were assessed by planimetric (wound contraction) and macroscopic parameters.

Results

Skin irritation tests showed that topical application of EOLS promoted cutaneous inflammation in varying degrees, which was demonstrated by increase of skin thickness and formation of cutaneous edema and erythema. Topical administration of EOLS in high concentrations presented an irritant response to skin, but this irritation is lighter when low concentrations this oil were used. Histological evaluation supported the outcome of these models, which revealed accentuated presence of inflammatory cells infiltration. In wound healing process, the lesions treated with EOLS showed intense edema and exsudation up to day 5, but there were not significant differences in the wound contraction on days 14 and 21. No immunohistochemical staining was verified to COX-2 and VEGF mediators in skin treated with EOLS 12%.

Conclusion

The continuous application of EOLS in adequate concentrations on cutaneous wounds increases inflammatory response without delay the lesions closure. The association of these results with antimicrobial action previously related to EOLS allows its indication as an alternative therapeutic modality for topical treatment of infected cutaneous wound. Nevertheless, further studies need to be performed to determine the mechanism of action and support its application in clinical practice.     

蜂蜜、蜂胶对大鼠皮肤溃疡创面愈合的影响

[目的]研究蜂蜜、蜂胶外用对皮肤溃疡模型大鼠创面愈合的影响。[方法]采用2.5 mol/L NaOH外涂建立大鼠皮肤溃疡模型。观察蜂蜜、蜂胶外用对溃疡创面愈合时间的影响,观察创面皮肤组织学变化,检测大鼠血清中超氧化物歧化酶(SOD)、丙二醛(MDA)的含量。[结果]蜂蜜、蜂胶外用能明显缩短创面愈合时间(P<0.05或P<0.01);能显著提高大鼠血清中SOD含量(P<0.05或P<0.01);能降低血清中MDA含量(P>0.05)。[结论]蜂蜜、蜂胶外用能够促进皮肤溃疡创面愈合。