系统性红斑狼疮患者抗磷脂抗体的检测

目的探讨抗磷脂抗体(APA)在正常人的分布及其与系统性红斑狼疮(SLE)的关系。方法应用酶联免疫吸附实验(ELISA)检测82例正常人和32例SLE患者血中6种APA。结果正常人IgG型抗心磷脂抗体(aCL)、抗磷脂酰肌醇抗体(aPI)、抗磷脂酸抗体(aPA)、抗磷脂酰乙醇胺抗体(aPE)、抗磷脂酰丝氨酸抗体(aPS)和抗磷脂酰胆碱抗体(aPC)的阳性率分别为6.10%、6.10%、7.32%、7.32%、3.66%和3.66%,IgM型aCL、aPA、aPC、aPS、aPE和aPI的阳性率分别为2.44%、3.66%、3.66%、6.10%、6.10%和4.88%。SLE患者IgG型aCL、aPA、aPS、aPC、aPI的A值和阳性率显著高于正常人,IgM型aCL、aPA、aPS、aPE、aPC的A值和阳性率显著高于正常人;SLE时IgG和IgM型APA的总阳性率分别为68.75%和71.88%。结论在正常人中存在滴度较低的APA;这些APA互相间有一定的关系,多同时出现。SLE时进行APA检查,对抗磷脂综合征的诊断可能有一定的意义。     

SLE患者血清抗磷脂抗体的分布及临床意义

目的:探讨抗磷脂抗体(APA)在SLE患者血清中的分布及临床意义。方法:应用酶联免疫吸附实验(ELISA)法检测64例正常人和56例SLE患者血清中6种APA。结果:SLE患者IgG型抗心磷脂抗体(aCL)、抗磷脂酰肌醇抗体(aPI)、抗磷脂酸抗体(aPA)、抗磷脂酰丝氨酸抗体(aPS)、抗磷脂酰胆碱抗体(aPC)的A值和阳性率均显著高于正常人;IgM型aCL、aPA、aPE、aPS、aPC的A值和阳性率均显著高于正常人。SLE时IgG型和IgM型APA的总阳性率分别为67.86%和69.65%。结论:正常人血清中存在低滴度的APA,SLE患者血清中APA的阳性率高于正常人。APA的检测对早期诊断SLE可能有一定意义。     

梅毒、红斑狼疮和皮肌炎患者抗磷脂抗体检测

目的：探讨抗磷脂抗体与梅毒、系统性红斑狼疮(SLE)和皮肌炎(DM)的关系。方法：应用酶联免疫吸附试验检测16例梅毒患者、32例SLE患者和11例皮肌炎患者血清中的抗磷脂抗体。结果：①梅毒患者抗磷脂抗体的吸光度（A值)[lgG型抗磷脂酰胆碱抗体(aPC)除外]和IgM型阳性率[抗磷脂酸抗体(aPA)除外]与正常人相比显著升高，IgG型抗磷脂抗体阳性率只有抗心磷脂抗体(aCL)和抗磷脂酸抗体(aPA)，与正常人相比显著升高；②SLE患者抗磷脂抗体的A值和阳性率[IgG型抗磷脂酰乙醇胺抗体(aPE)和IgM型抗磷脂酰肌醇抗体(aPI)除外1与正常人相比显著升高；③DM患者抗磷脂抗体的A值[IgG型抗磷脂酰乙醇胺抗体(aPE)除外1与正常人相比显著升高。④梅毒和SLE患者总阳性率与正常人相比显著升高。结论：自身免疫性疾病患者的抗磷脂抗体与感染时有所不同；抗磷脂抗体虽然存在于多种自身免疫性疾病中，但以SLE的阳性率最高。     

梅毒与系统性红斑狼疮患者抗心磷脂抗体的比较研究

目的 对梅毒和系统性红斑狼疮(SLE)患者血清中抗心磷脂(ACL)抗体的反应强度以及类型进行比较研究。方法 应用ELISA法对99例梅毒和75例SLE患者的血清中ACL抗体的反应强度以及ACL抗体的IgG与IgM类型进行检测。结果 SLE和梅毒患者血清中IgG型ACL抗体阳性率分别为48.00%和40.40%;梅毒的阳性血清反应强度高于SLE患者(P<0.001);IgM型ACL抗体的阳性率在SLE和梅毒患者中分别为18.67%和61.62%,梅毒的ACL抗体阳性率显著高于SLE患者(P<0.001),且梅毒患者IgM型ACL抗体阳性血清反应强度高于SLE患者(P<0.005)。结论 这种ACL抗体IgG、IgM阳性率与反应强度的差异,反映了SLE患者和梅毒患者产生ACL抗体的机理与功能有所不同。     

银屑病患者血清抗角蛋白抗体的免疫荧光研究

为了探讨血清抗角蛋白抗体与银屑病的关系以及该抗体的生理、病理意义,本文以正常人皮肤冰冻切片为抗原,用IIF检测了35例银屑病患者的血清,并与其它皮肤病和内科疾患作了对照观察.银屑病患者血清抗角蛋白抗体阳性率IgG11.76%,IgM54.29%,IgA54.29%,与正常人(IgG66,67%,IgM96.43%,IgA100%)相比,均明显降低.但23例其它皮肤病IgM60.85%,IgA43%和25例内科疾患IgM63.38%也明显低于正常人.结果提示,抗角蛋白抗体血清阳性率的降低,并非银屑病的特异表现,两者之间并无特殊联系.     

红斑、丘疹及鳞屑性皮肤病

971414 寻常性银屑病患者血浆肿瘤坏死因子水平检测/王惠芳…//中国皮肤性病学杂志.-1996,10（6）.-327 检测40例寻常性银屑病患者血浆中肿瘤坏死因子（TNF）含量及IgG、IgA、IgM与C_3水平,以探讨TNF在银屑病发病中的意义。对照组为43例正常人。结果:银屑病组血浆TNF较正常对照组显著升高。男性高于女性（P<0.05）,进行期高于静止期,伴甲部损害者TNF明显升高。TNF水平与病情严重程度呈正相关,与IgA、IgG、IgM、C_3无明显线性关系。本实验结果证实银屑病患者TNF血浆含量高于正常人46倍。40例中血浆TNF最低值均明显高于正常人,提示TNF参与了银屑病的病理生理过程。而TNF含量高低与疾病预后有一定关系。（刘辅仁）971415 银屑病患者皮损和正常皮肤中Ig和C_3的免     

病毒性皮肤病

951983 单纯疱疹病毒2型血清IgG及IgM抗体的酶免疫检测/钱起丰…//中华皮肤科杂志。-1995,28(3).-185～186 为了解发病情况,采用德国Virotech公司生产的HSV-2 IgG及IgM抗体酶免疫试剂检测生殖器疱疹患者60例和40例正常人。结果HSV-2抗体总阳性率为88.3％(53/60),其中IgG抗体阳性率为73.3％(44/60),IgM抗体阳性率为20.0％(12/60),其中有3例两者均为阳性。40例正常人分别为20％(8/40)及0,与患者组相比差异有显著性(P<0.01)。初发型与     

140例疑似生殖器疱疹患者单纯疱疹病毒Ⅱ型抗原和抗体检测结果分析

目的：探讨单纯疱疹病毒Ⅱ型（HSV-2）抗原和抗体检测对生殖器疱疹的诊断意义。方法：对性病门诊140例有新发水疱的现症生殖器疱疹（GH）病人用ELISA方法进行单纯疱疹病毒Ⅱ型（HSV-2）抗原和抗体检测，了解现症GH病人HSV-2抗原和抗体之间的关系。结果：对于有新发水疱的现症GH病人HSV-2抗原检测阳性率为89．3％，而HSV-2IgG阳性率为66．4％，HSV-2IgM阳性率为14．5％。对初发病人：HSV-2抗原阳性率为89．7％（70／78）；HSV-2IgG阳性率为39．7％（31／78）；HSV-2IgM阳性率为25．6％（20／78）。对复发病人：HSV-2抗原阳性率为88．7％（55／62）；HSV-2IgG阳性率为100％（62／62）；HSV-2IgM阳性检出率为0。结论：对于有新发水疱的现症GH病人HSV-2抗原检测阳性检出率较高。而HSV-2IgM抗体作为诊断指标仍有待进一步探讨。     

银屑病患者血清中抗溶血性链球菌抗体的类别及其意义

目的：探讨银屑病患者血清中抗β－溶血性链球菌抗体的类别及其意义。方法：用ELISA法测定各型银屑病患者及正常对照血清中抗β－溶血性链球菌相应的抗体IgG、IgM、IgA。结果：各型银屑病患者血清中存在不同水平的抗β－溶血性链球菌之相应的IgG、IgM、IgA类抗体；与正常对照相比，IgM类抗体各型银屑病均高于正常对照，并有统计学差异（P＜0.05）；银屑病各型之间的比较，点滴状和斑块状银屑病与其余型别不同，IgG类抗体占优势。结论：在各型银屑病中虽然抗β－溶血性链球菌各类别抗体水平不同，但趋势一致，特别是IgM类抗体的检测提示此微生物在银屑病病因的可能一致性，其中以点滴状银屑病为一典型的代表。     

银屑病患者血清抗EB病毒抗体的检测

目的：探讨EB病毒感染与银屑病的关系。方法：采用酶免疫法检测53例银屑病患者血清EB病毒抗壳抗原／IgG、IgM抗体，抗早期抗原－D／IgG抗体。抗核心抗原－1／IgG抗体。结果：银屑病患者血清抗ER病毒早期抗原－D／IgG抗体阳性率明显高于正常对照组（P＜0．001）。结论：银屑病患者体内EB病毒可能处于激活状态。     

New fillers for the new man

ABSTRACT:  Two new collagen-based lidocaine-containing dermal fillers, ArteSense™/ArteFill™ (Artes Medical, San Diego, CA) and Evolence® (Colbar LifeScience Ltd., Herzliya, Israel), have proved to be of particular interest to men, many of whom seek a long-lasting or permanent correction. ArteFill™ has been available in the United States since 2006, and it is expected that Evolence® will reach the American market in 2008. The properties of the two products will be described, and experience based on the administration of many hundreds of syringes of both products by a Canadian dermatologist will be detailed here, with tips and precautions to optimize patient outcomes.     

Outcomes and adverse effects of ablative vs nonablative lasers for skin resurfacing: A systematic review of 1093 patients

It is generally believed that ablative laser therapies result in prolonged healing and greater adverse events when compared with nonablative lasers for skin resurfacing. To evaluate the efficacy of ablative laser use for skin resurfacing and adverse events as a consequence of treatment in comparison to other modalities, a PRISMA‐compliant systematic review (Systematic Review Registration Number: 204016) of twelve electronic databases was conducted for the terms “ablative laser” and “skin resurfacing” from March 2002 until July 2020. Studies included meta‐analyses, randomized control trials, cohort studies, and case reports to facilitate evaluation of the data. All articles were evaluated for bias. The search strategy produced 34 studies. Of 1093 patients included in the studies of interest, adverse events were reported in a total of 106 patients (9.7%). Higher rates of adverse events were described in nonablative therapies (12.2% ± 2.19%, 31 events) when compared with ablative therapy (8.28% ± 2.46%, 81 events). 147 patients (13.4%) reported no side effects, 68 (6.22%) reported expected, transient self‐resolving events, and five (0.046%) presented with hypertrophic scarring. Excluding transient events, ablative lasers had fewer complications overall when compared with nonablative lasers (2.56% ± 2.19% vs 7.48% ± 3.29%). This systematic review suggests ablative laser use for skin resurfacing is a safe and effective modality to treat a range of pathologies from photodamage and acne scars to hidradenitis suppurativa and posttraumatic scarring from basal cell carcinoma excision. Further studies are needed, but these results suggest that ablative lasers are a superior, safe, and effective modality to treat damaged skin.     

Genetic alterations in RAS‐regulated pathway in acral lentiginous melanoma

Studies integrating clinicopathological and genetic features have revealed distinct patterns of genomic aberrations in Melanoma. Distributions of BRAF or NRAS mutations and gains of several oncogenes differ among melanoma subgroups, while 9p21 deletions are found in all melanoma subtypes. In the study, status of genes involved in cell cycle progression and apoptosis was evaluated in a panel of 17 frozen primary acral melanomas. NRAS mutations were found in 17% of the tumors. In contrast, BRAF mutations were not found. Gains of AURKA gene (20q13.3) were detected in 37.5% of samples, gains of CCND1 gene (11q13) or TERT gene (5p15.33) in 31.2% and gains of NRAS gene (1p13.2) in 25%. Alterations in 9p21 were identified in 69% of tumors. Gains of 11q13 and 20q13 were mutually exclusive, and 1p13.2 gain was associated with 5p15.33. Our findings showed that alterations in RAS‐related pathways are present in 87.5% of acral lentiginous melanomas.     

Self‐resolving superficial primary cutaneous mucormycosis in a 7‐week‐old infant

A 7‐week‐old girl, born at 30 weeks' gestational age, presented to clinic for evaluation of a crop of vesicular lesions that were noted after removal of a bandage that had been in place for 4 days. A punch biopsy of the lesion revealed fungal elements that were later identified as Rhizopus spp. The lesion began to self‐resolve, and no further treatment was needed, with full resolution of the lesion by 1 month after presentation. Clinicians should be aware of the variable presentations of mucormycosis and consider fungal infection in the differential diagnosis when evaluating vulnerable patients with skin eruptions.     

Multiple New-Onset Subcutaneous Nodules of the Upper Extremity Digits: Giant Cell Tumor of Tendon Sheath and Lipoma

A black woman with the concurrent onset of two subcutaneous nodules located on the digits of her upper extremities is described. Initially, a single systemic disorder was considered; yet, the lesions differed in morphology and consistency. Microscopic examination of the nodules showed a giant cell tumor of tendon sheath and a lipoma. Although Occam's “razor” suggests that multiple lesions in the same person are more likely to represent variable manifestations of a single disorder than several different diseases in that individual, the simultaneously appearing lesions in this patient represented two different conditions.