Declining sensitivity to interleukin 3 of murine multipotential hemopoietic progenitors during their development. Application to a culture system that favors blast cell colony formation.

When spleen cells of 5-fluorouracil (5-FU)-treated mice were cultured in the presence of interleukin 3 (IL-3), most colonies revealed IL-3 concentration-dependent colony formation except for mast cell colonies and blast cell colonies. While most colonies were smaller in lower concentrations of IL-3, the size of the blast cell colonies were similar between high and low IL-3 groups. These data suggested that blast cell colony development requires less IL-3 than the development of multilineage colonies from blast cell colonies. This notion was supported by experiments in which IL-3 was added twice to cultures of spleen cells of 5-FU-treated mice. When low concentrations of IL-3 were added on day 7, there was a reduction in the number of multilineage colonies formed without an effect on the number of blast cell colonies. Using this information, we developed a culture system that favors blast cell colony formation by cells of normal mice. When low (20 U/ml) concentrations of IL-3 were added to cultures of spleen cells of normal mice on day 7 of incubation in media containing 2-5% fetal calf serum, blast cell colonies were the predominant colony type. The blast cell colonies revealed high but variable secondary replating efficiencies. These data suggest that multipotential progenitors may become less sensitive to IL-3 as they differentiate in culture.     

Hemopoietic colony-forming cells in umbilical cord blood with extensive capability to generate mono- and multipotential hemopoietic progenitors.

We report identification of a unique class of human hemopoietic colony-forming cells with extensive ability to generate progenitors for secondary colonies. Mononuclear cells isolated from human umbilical cord blood formed colonies consisting of 40-500 blast cells after 25 d of incubation in methylcellulose culture in the presence of erythropoietin and medium conditioned by phytohemagglutinin-stimulated leukocytes. Replating of these blast cell colonies revealed that 100% of the primary colonies had the ability to generate secondary colonies, including multipotential colonies. These colonies could be distinguished from other hemopoietic colonies in situ by the complete absence of signs of terminal differentiation. Replating of granulocyte-erythrocyte-macrophage-megakaryocyte (GEMM) colonies, consisting of an average of 2 x 10(4) cells, revealed less capacity for secondary colony formation. This human blast cell colony assay may provide a method for quantitation of more primitive hemopoietic stem cells than progenitors for GEMM colonies (CFU-GEMM) in man.     

Procalcitonin and interleukin 6 for predicting blood culture positivity in sepsis

Background

Procalcitonin and interleukin 6 (IL-6) are well-known predictors of blood culture positivity in patients with sepsis. However, the association of procalcitonin and IL-6 with blood culture positivity was assessed separately in previous studies. This study aims to examine and compare the performance of procalcitonin and IL-6, measured concomitantly, in predicting blood culture positivity in patients with sepsis.

Methods

Forty adult patients with sepsis were enrolled in the study. Blood cultures were drawn before the institution of antibiotic therapy. The area under the curve (AUC) of the receiver operating characteristic curve was estimated to assess the performance of procalcitonin and IL-6 in predicting blood culture positivity.

Results

Positive blood cultures were detected in 10 patients (25%). The AUC of procalcitonin and IL-6 was 0.85 and 0.61, respectively. The combined performance of procalcitonin and IL-6 was similar to that of procalcitonin alone, AUC of 0.85. On univariate analysis, only procalcitonin and IL-6 were associated with blood culture positivity. Multivariate logistic regression analysis showed that only procalcitonin was associated with blood culture positivity (odds ratio, 12.15 [1.29-114.0] for levels above the median compared with levels below the median). Using procalcitonin cut points of 1.35 and 2.14 (nanogram per milliliter) enabled 100% and 90% identification of positive blood cultures and reduced the need of blood cultures by 47.5% and 57.5%, respectively.

Conclusions

Compared with IL-6, procalcitonin better predicts blood culture positivity in patients with sepsis. Using a predefined procalcitonin cut points will predict most positive blood cultures and reduce the need of blood cultures in almost half of patients with sepsis.     

The effects of recombinant interleukin 2-activated natural killer cells on autologous peripheral blood hematopoietic progenitors

In the present study, we demonstrate that resting and rIL-2-activated NK cells had no inhibitory effects on peripheral blood-derived hematopoietic progenitor (HP) cells. Peripheral blood HP cells were similar to bone marrow progenitors in phenotype and clonogenic colony formation capabilities. Peripheral blood HP cells could be cocultured in vitro with rIL-2-activated autologous NK cells for 3 d without adverse effects on the HP cells. Acute myelogenous leukemia patients in stable remission were shown to have normal percentages of NK cells and elevated percentages of peripheral blood HP cells. NK cells from most of these patients could be activated with rIL-2 to lyse fresh uncultured tumor cells as well as autologous leukemia cells without effecting the peripheral blood HP cells. These results suggest that rIL-2-activated NK cells may be used to purge peripheral blood HP cell preparations of residual tumor cells before hematopoietic reconstitution.     

The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells

The role of recombinant B cell stimulatory factor 2 (BSF-2/IL-6) in the regulation of growth and differentiation of B cells was investigated. rBSF-2 at 200 pg/ml could induce 50% of the maximum Ig production in B lymphoblastoid cell lines, the specific activity being estimated as 5 X 10(6) U/mg. rBSF-2 augmented PWM-induced IgM, IgG, and IgA production in mononuclear cells (MNC); the effect was exerted by directly acting on PWM-induced B blast cells to induce Ig production. However, rBSF-2 did not induce any growth of activated B cells. In contrast, rBSF-2 showed a potent growth activity on a murine hybridoma clone, MH60.BSF2. The concentration required for half-maximal [3H]TdR uptake was approximately 5 pg/ml, which was 40 times less than that required for Ig induction in a B cell line. Anti-BSF-2 antibody inhibited PWM-induced Ig production in MNC, but not PWM-induced proliferation. The antibody was effective even when added on day 4 of an 8-d culture, indicating that BSF-2 is one of the essential late-acting factors in PWM-induced Ig production.     

氯氮平和利培酮对首发精神分裂症患者细胞免疫因子的影响

目的了解氯氮平、利培酮对首发精神分裂症患者血清白细胞介素-2(IL-2)、白细胞介素-6(IL-6)的影响。方法对62例首发精神分裂症患者给予氯氮平或利培酮治疗,分别在治疗前和治疗后第4、8周末、6个月末用酶联免疫吸附法检测血清IL-2、IL-6水平及其变化。结果两组患者治疗后第4、8周末血清IL-2、IL-6水平均显著低于治疗前(P<0.05);不同药物对IL-2、IL-6影响差异无显著性(P>0.05)。结论氯氮平和利培酮对IL-2、IL-6均有抑制作用,利培酮对IL-2、IL-6的影响大一些。     

Effects of 2',3'-dideoxynucleosides on proliferation and differentiation of human pluripotent progenitors in liquid culture and their effects on mitochondrial DNA synthesis.

2',3'-Dideoxynucleosides (ddNs) including 3'-azido-3'-deoxythymidine (AZT), 3'-fluoro-3'-deoxythymidine (FLT), 3'-amino-3'-deoxythymidine (AMT), 2',3'-dideoxycytidine (ddC), and 2',3'-didehydro-3'-deoxythymidine (D4T) were tested for their effects on proliferation and differentiation of pluripotent progenitor cells (CD34+) purified from human bone marrow cells grown in liquid cultures. These highly purified progenitor cells undergo extensive proliferation during 14 days, with a marked differentiation during the last 7 days. These differentiated cells exhibit normal morphological features in response to specific hematopoietic growth factors of both erythroid and granulocyte-macrophage lineages, as demonstrated by flow cytometry cell phenotyping. The potencies of these ddNs in inhibiting proliferation of granulocyte-macrophage lineage cells were in the order FLT > AMT = ddC > AZT >> D4T, and the potencies in inhibiting proliferation of erythroid lineage cultures were in the order FLT > AMT > AZT > ddC >> D4T. The toxic effects of ddNs assessed in these liquid cultures were in agreement with data obtained by using semisolid cultures, demonstrating the consistency of these two in vitro hematopoietic systems toward ddN toxicity. ddC was toxic to CD34+ progenitor cells and/or cells in the early stages of differentiation, whereas the inhibitory effect of AZT on the erythroid lineage was predominantly observed on a more mature population of erythroid progenitors during the differentiation process. Slot blot analysis of granulocyte-macrophage cultures demonstrated that exposure to ddC and FLT was associated with a decrease in total mitochondrial DNA (mtDNA) content, suggesting that these two ddNs inhibit mtDNA synthesis. In contrast, no difference in the ratio of nuclear DNA to mtDNA was observed in cells exposed to toxic concentrations of AZT and AMT is not associated with an inhibition of mtDNA synthesis. This human pluripotent progenitor liquid culture system should permit detailed investigations of the cellular and molecular events involved in ddN-induced hematological toxicity.     

The activity of natural cytotoxic cells is augmented by interleukin 2 and interleukin 3

Murine natural killer (NK) and natural cytotoxic (NC) cells showed different patterns of augmentation of lytic activity after preincubation for 24 h with either poly-IC, interleukin 2 (IL-2), or interleukin 3 (IL-3): (a) Poly-IC augmented only NK cells, with no effect on NC activity, as we have previously observed (4); (b) IL-2 augmented both NK and NC activity; and (c) IL-3 augmented only NC lysis, without affecting NK activity. In addition, both precursor and the augmented effector cells showed differences in expression of the Qa- 5 surface marker: NK precursors and effectors are Qa 5+, whereas NC precursors and effector cells are Qa-5-.     

热疗对荷VX2瘤兔血清白细胞介素2,6,8的影响

目的探讨热疗对荷VX2瘤兔血清细胞因子的影响.方法实验于2004-12/2005-07在大同市第七人民医院实验室进行.选健康雄性大白兔40只,随机分为热疗组20只、对照组20只.所有动物进行VX2瘤株移植,接种后11d,实验动物右后肢均可触及平均直径为2.5 cm大小的肿瘤.对照组不干预,热疗组动物进行热疗照射(采用HG-2000体外高频热疗机,输出功率在90%～95%,电压为200V,温度控制在43℃左右),时间为10min,共15 d,1次/d.在VX2瘤株移植前、治疗前和治疗后1,3,4周监测动物血中白细胞介素2,6,8的变化,同时观察两组动物肿瘤大小及存活时间.结果40只动物全部进入结果分析.①白细胞介素2两组兔治疗后3,4周均较治疗前降低(P＜0.05,0.01),但热疗组显著高于对照组[(61.85±2.02),(41.76±2.27)μg/L;(54.77±2.55),(35.21±1.60)μg/L,P＜0.01].②白细胞介素6两组兔治疗后3,4周均较治疗前升高(P＜0.05,0.01),但热疗组显著低于对照组[(5.42±0.19),(6.41±0.18)μg/L;(5.89±0.12),(6.96±0.24)μg/L,P＜0.01].③白细胞介素8两组兔治疗后4周均较治疗前降低(P＜0.05),但两组间无差异.④热疗组动物生存期较对照组延长15 d左右,肿瘤的局部转移也较对照组少.结论热疗对于肿瘤的治疗是有用的辅助疗法;细胞因子的变化可能是直接杀死肿瘤细胞诱发机体免疫功能的作用,或与加热后瘤细胞产生调亡及热休克蛋白相关.     

热疗对荷VX2瘤兔血清白细胞介素2,6,8的影响

目的：探讨热疗对荷VX2瘤兔血清细胞因子的影响.方法：实验于2004-12/2005-07在大同市第七人民医院实验室进行.选健康雄性大白兔40只,随机分为热疗组20只、对照组20只.所有动物进行VX2瘤株移植,接种后11d,实验动物右后肢均可触及平均直径为2.5 cm大小的肿瘤.对照组不干预,热疗组动物进行热疗照射（采用HG-2000体外高频热疗机,输出功率在90%～95%,电压为200V,温度控制在43℃左右）,时间为10min,共15 d,1次/d.在VX2瘤株移植前、治疗前和治疗后1,3,4周监测动物血中白细胞介素2,6,8的变化,同时观察两组动物肿瘤大小及存活时间.结果：40只动物全部进入结果分析.①白细胞介素2：两组兔治疗后3,4周均较治疗前降低（P＜0.05,0.01）,但热疗组显著高于对照组[（61.85&;#177;2.02）,（41.76&;#177;2.27）μg/L;（54.77&;#177;2.55）,（35.21&;#177;1.60）μg/L,P＜0.01].②白细胞介素6：两组兔治疗后3,4周均较治疗前升高（P＜0.05,0.01）,但热疗组显著低于对照组[（5.42&;#177;0.19）,（6.41&;#177;0.18）μg/L;（5.89&;#177;0.12）,（6.96&;#177;0.24）μg/L,P＜0.01].③白细胞介素8：两组兔治疗后4周均较治疗前降低（P＜0.05）,但两组间无差异.④热疗组动物生存期较对照组延长15 d左右,肿瘤的局部转移也较对照组少.结论：热疗对于肿瘤的治疗是有用的辅助疗法;细胞因子的变化可能是直接杀死肿瘤细胞诱发机体免疫功能的作用,或与加热后瘤细胞产生调亡及热休克蛋白相关.     

Association between nucleated red blood cells in blood and the levels of erythropoietin, interleukin 3, interleukin 6, and interleukin 12p70

The appearance of nucleated red blood cells (NRBC) in the circulation is associated with a variety of severe diseases, and indicates a relatively poor prognosis. Whether a malfunction of the bone marrow leads to this phenomenon is as unknown as the possible role that cytokines could play in this process. We analyzed erythropoietin, interleukin (IL)-3, IL-6, and IL-12p70 in the blood of 301 patients with circulating NRBCs. Two hundred fifty NRBC-negative patients served as controls. Multiple logistic regression revealed a significant association between the appearance of NRBCs in the blood and erythropoietin (odds ratio, 1.017; 95% confidence limits, 1.007-1.027; P < 0.001), IL-3 (odds ratio, 1.293; 95% confidence limits, 1.180-1.417; P < 0.001), IL-6 (odds ratio, 1.138; 95% confidence limits, 1.016-1.275; P < 0.05), and age (odds ratio, 1.019; 95% confidence limits, 1.009-1.030; P < 0.001), respectively. Gender and IL-12p70 were not significantly associated with the appearance of NRBC in the blood. To estimate the RBC production in the bone marrow, the increase in the reticulocyte concentration in blood was measured. The reticulocyte concentration in NRBC-positive patients was 69 +/- 2/nL, which was significantly higher than in NRBC-negative patients (60 +/- 2/nL; P < 0.01). Taken together, NRBC could be a marker that sums up hypoxic and inflammatory injuries. Thus, generally, the appearance of NRBC in blood is a valid parameter to identify patients at high mortal risk. Moreover, the increased number of reticulocytes in the blood of NRBC-positive patients may indicate that the appearance of NRBC is not associated with disturbed bone marrow function as far as the erythropoiesis is concerned.     

The early progenitors of mouse dendritic cells and plasmacytoid predendritic cells are within the bone marrow hemopoietic precursors expressing Flt3

Flt3 ligand (Flt3L) is a growth factor for hemopoietic progenitors and can promote the expansion of both conventional dendritic cells (DCs) and plasmacytoid predendritic cells (p-preDCs). The cells responding to Flt3L treatment and the precursors for the DCs and p-preDCs had not been fully characterized. We examined different mouse bone marrow (BM) hemopoietic precursor populations for the surface expression of Flt3 and tested them for early DC and p-preDC precursor activity. Most DC precursor activity, other than that given by multipotent hemopoietic stem cells, was within the downstream precursors expressing Flt3. The majority of mouse BM common lymphoid precursors expressed high levels of Flt3 and these were the most efficient precursors of both DCs and p-preDCs. In contrast, only a small proportion of the common myeloid precursors (CMPs) expressed Flt3, but the precursor activity for both DCs and p-preDCs was within this minor Flt3+ CMP fraction. The granulocyte and macrophage precursors and pro-B cells did not express Flt3 and had no DC or p-preDC precursor activity. These findings demonstrate that the early precursors for all DC subtypes are within the BM Flt3+ precursor populations, regardless of their lymphoid or myeloid lineage orientation.     

血液透析对慢性肾功能衰竭患者血清SIL2R、IL6和TNFα的影响

目的：探讨慢性肾功能不全（ＣＲＦ）患者细胞免疫功能状况,以及血液透析（血透）的影响。方法：应用酶联免疫吸附（ＥＬＩＳＡ）双抗体夹心法检测３０例ＣＲＦ未透析患者和６２例血透患者血透前后及对照组血清可溶性白介素－α受体（ＳＩＬ－２Ｒ）、白介素－６（ＩＬ－６）和肿瘤坏死因子－α（ＴＮＦ－α）水平。结果：与对照组相比,ＣＲＦ未透析患者和血透患者血清ＳＩＬ－２Ｒ、ＩＬ－６和ＴＮＦ－α水平无显著差异。血清ＳＩＬ－２Ｒ较透前显著增高。结论：ＣＲＦ患者均存在显著的细胞免疫功能紊乱,单次透析对细胞免疫功能无显著影响。     

Deficient interleukin 2 activity in MRL/Mp and C57BL/6J mice bearing the lpr gene

Spleen cells from MRL-lpr and B6-lpr mice have a marked defect in the ability to produce interleukin 2 (IL-2) in response to concanavalin A stimulation. This defect precedes the onset of clinical illness, increases with age, and eventually becomes virtually absolute. It is not due to cellular suppression of IL-2 production, nor does it reflect the presence of a soluble inhibitor of IL-2 activity. Failure to restore IL-2 production with macrophage-replacing factors, such as interleukin 1 and phorbol myristic acetate, suggests that IL-2 deficiency reflects a primary T cell defect rather than a macrophage defect. MRL-lpr and B6-lpr spleen cells also have an age-dependent reduction in IL-2 response that apparently results from a deficiency of cell surface receptors for IL-2. Congenic MRL-+/+ and B6-+/+ mice, which lack the lpr gene responsible for accelerated autoimmunity and lymphoproliferation, have normal IL-2 activity. These findings suggest that a defect in IL-2 activity may contribute to impaired immunoregulation in mice bearing the lpr gene. The absence of such a defect in MRL-+/+ and B6-+/+ mice further suggests that a single autosomal recessive gene is responsible for IL-2 deficiency.     

Effect of steroids on lipopolysaccharide/interleukin 2-induced interleukin 18 production in peripheral blood mononuclear cells

Interleukin (IL) 18, a powerful inducer of the immunoregulatory cytokine interferon-gamma (IFN-gamma), presents upstream of the cytokine activation cascade in the inflammatory response. The anti-inflammatory properties of steroids permit their use in various conditions, although effects are transient and pathological states are not fully relieved by short-term steroidal use. We examined the effect of lipopolysaccharide (LPS)/IL-2 on the cytokine cascade in human peripheral blood mononuclear cells (PBMCs). We also examined the effect of steroids on LPS/IL-2-induced cytokine production in human PBMCs taken from healthy volunteers. Cell-free supernatant fractions were assayed for IL-18, IL-12, IL-2, IFN-gamma and IL-10 protein, using enzyme-linked immunosorbent assays, and synergy between LPS and IL-2 in enhanced production of IL-18 was observed. Steroids suppressed the production of IL-18 and other secondary cytokines in LPS/IL-2-stimulated PBMCs, in a concentration- and time-dependent manner, although inhibition was incomplete even at high concentrations. Effects of steroid treatment on expression of membrane-bound LPS receptor antigen (mCD14) and intercellular adhesion molecule-1 (ICAM-1) in PBMCs were studied by flow cytometric analysis. Steroid treatment up-regulated mCD14 expression in a concentration-dependent manner, with no effect on ICAM-1 expression. These results suggest that the incomplete counteraction of steroids in the LPS/IL-2-initiating cytokine cascade is due, at least partly, to the up-regulation of mCD14 by steroid preparations, which increases susceptibility to bacterial endotoxins.     

Histiocytosis X. Purified (T6+) cells from bone granuloma produce interleukin 1 and prostaglandin E2 in culture.

We have investigated the secretory function of cell suspensions from bone eosinophilic granulomas surgically collected in two patients with histiocytosis X. Unseparated cell preparations spontaneously produced interleukin 1 (IL-1) and prostaglandin E2 (PGE2). In order to ascertain that this secretion was due to the characteristic Langerhans cell-like histiocytosis X cells predominantly found in the bone lesions, we have purified T6+ cells by the use of a fluorescence-activated cell sorter. Such highly purified cell preparations were found to secrete IL-1 and PGE2 spontaneously in culture. Stimulation with endotoxins and treatment with interferon gamma (IFN gamma) revealed an intense IL-1 secretory function of histiocytosis X cells. Since both IL-1 and PGE2 are able to induce bone resorption in vitro, our findings are compatible with the hypothesis that histiocytosis X cells are responsible for the typical osteolytic lesion observed in histiocytosis X through the local secretion of these two mediators.     

氯氮平对不同病程女性精神分裂症患者血清白细胞介素 6及可溶性白细胞介素 6受体的影响

目的探讨女性精神分裂症患者血清白细胞介素 6(IL 6)、可溶性白细胞介素 6受体 (sIL 6R)治疗前后变化及其与抗精神病药物氯氮平血清水平的关系. 方法采用酶联免疫吸附法测定 40例女性精神分裂症患者 (病程≥ 5年组和病程 < 5年组,每组 20例 )治疗前及治疗后第 1、 2和 4周血清 IL 6和 sIL 6R水平,同时用高效液相色谱法测定氯氮平水平,以 20例女性健康者血清 IL 6、 sIL 6R水平作对照.治疗前与治疗后第 4周评定阴性和阳性症状量表 (PANSS)各一次. 结果①病程≤ 5年组患者治疗前血清 IL 6和 sIL 6R水平均显著高于正常对照组 (P< 0.01);治疗后第 1、 2和 4周血清 IL 6和 sIL 6R水平均显著低于对照组 (P< 0.01).②病程 >5年组患者治疗前及治疗后第 1,2和 4周血清 IL 6、 sIL 6R水平均显著低于正常对照组( P< 0.01).③除病程 >5年组患者第 1周血清 IL 6水平与氯氮平水平呈负相关( r=0.57,P< 0.01)外,余各时点血清 sIL 6R水平与氯氮平水平均无显著相关性( r=- 0.36～ 0.17,P >0.05).④氯氮治疗 4周后, PANSS减分率与 IL 6,sIL 6R减分率无显著相关( r=- 0.22～- 0.01,P >0.05). 结论女性精神分裂症患者血清 IL 6、 sIL 6R水平与健康女性差异显著 ,氯氮平对 IL 6和 SIL 6R有抑制作用,氯氮平的抗精神病作用可能与对 IL 6,sIL 6R的调节作用有关.     

脐血输注对妇科恶性肿瘤患者外周血中IL-2、IL-6和sIL-2R的诱导表达和T细胞亚群改变

目的　探讨妇科恶性肿瘤患者免疫功能的变化机制及脐血输注对其的影响。方法　将化疗后妇科恶性肿瘤患者 30例分为脐血治疗组 15例、非脐血治疗组 15例 ,并设正常人对照组 30例 ,采用MTT法、ELISA法和流式细胞仪检测的方法观察脐血输注后患者免疫功能变化。结果　①患者外周血CD4 + 细胞含量低于正常人、CD8+细胞含量高于正常人 (t均为 2 .5 6 ,P <0 .0 5 ) ,CD4 + /CD8+ 比例倒置 ;脐血治疗组 30名脐血输注后患者CD3+ 细胞含量明显升高 (t=3.0 1,P <0 .0 1)、CD4 + 细胞含量明显升高 (t=2 .74 ,P <0 .0 5 ) ,CD4 /CD8比例恢复正常。②30名患者外周血IL 2活性较正常人降低 ,可溶性IL 2R(sIL 2R)含量明显增加 (t分别为 4 .33、5 .89,P <0 .0 1) ;脐血输注后患者IL 2活性增加 ,sIL 2R含量降低 ,与对照组相比 ,差异有显著性 (t分别为 2 .32、2 .4 2 ,P <0 .0 5 )。而IL 6活性有一定程度下降 ,但无统计学意义 (t=0 .2 7,P >0 .0 5 )。结论　妇科恶性肿瘤患者的免疫功能低于正常人 ,脐血输注后可以通过免疫活性细胞与淋巴因子水平的变化调节患者免疫功能。     

心肌康对白介素-2和6在病毒性心肌炎中的作用研究

目的 :探讨病毒性心肌炎 (VMC)免疫发病机制及心肌康对 VMC小鼠血白介素 2和 6 (IL 2、IL 6 )水平的调节作用。方法 :腹腔注射柯萨奇 B3 病毒复制小鼠 VMC模型。将昆明种小鼠随机分为正常对照组、模型组、生脉散组及心肌康大、中、小剂量治疗组 ,用放射免疫法测定各组小鼠血清 IL 2、IL 6含量。结果 :与正常对照组比较 ,模型组 IL 2、IL 6水平明显升高 ,而生脉散组及心肌康治疗组小鼠血 IL 2、IL 6水平明显低于模型组。结论 :IL 2、IL 6分泌增加在 VMC急性期发病机制中起着重要作用。心肌康能有效地改善并调节 VMC感染机体的免疫功能 ,为治疗 VMC安全、有效的手段。