共查询到19条相似文献,搜索用时 203 毫秒
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目的:建立活血止痛散和活血止痛胶囊中三七的专属性鉴别方法,并采用高分离度快速液相色谱-三重串联四极杆质谱(RRLC-QQQ/MS)对结果加以验证。方法:采用高效薄层色谱法研究三七与其混伪品人参、红参、三七茎叶的特征条带。以高效硅胶G薄层板为固定相,二氯甲烷-无水乙醇-水(70:45:6.5)展开,10%硫酸乙醇溶液显色,105℃加热至条带清晰。分别置紫外光灯(365 nm)和日光下检视。RRLC-QQQ/MS分析采用Agilent Rapid Resolution HT SB-C18(2.1 mm×100 mm,1.8 μm)色谱柱,以5 mmol·L-1醋酸铵溶液-乙腈为流动相梯度洗脱。离子化模式为ESI-,碎裂电压100 V。结果:三七可检出三七皂苷R1,未检出人参皂苷Rb3、人参皂苷Rf。人参和红参可检出人参皂苷Rb3和人参皂苷Rf,未检出三七皂苷R1。三七茎叶可检出人参皂苷Rb3,未检出三七皂苷R1或人参皂苷Rg1。以三七对照药材、三七皂苷R1(应检出)和人参皂苷Rb3(不得检出)为对照,可实现活血止痛制剂中三七的专属性鉴别和人参(红参)或三七茎叶的非法掺杂投料检查。6个厂家的93批样品均未发现人参、红参或三七茎叶冒充三七非法投料。RRLC-QQQ/MS与高效薄层分析结果一致。结论:该方法简便、快速、灵敏、准确,可为含三七中成药的质量控制提供参考。 相似文献
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摘 要 目的: 建立同时测定人参三七颗粒中人参皂苷Rg1、Re、Rb1和三七皂苷R1含量的方法。方法: 采用HPLC法,色谱柱为Sunfire C18(150 mm×4.6 mm,5 μm)分析柱,流动相以乙腈-水梯度洗脱;检测波长为203 nm;柱温30℃;流速1.0 ml·min-1。结果:人参皂苷Rg1,Re,Rb1和三七皂苷R1之间有较好的分离度,4种成分在线性范围内与峰面积之间线性关系良好,人参皂苷Rg1、Re、Rb1和三七皂苷R1加样回收率分别为99.83%,97.84%,98.43%,97.34%,RSD分别为2.08%,1.66%,1.73%和1.42%(n=5)。结论:本方法可同时测定人参三七颗粒中的人参皂苷Rg1,Re,Rb1和三七皂苷R1含量。 相似文献
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目的 建立同时测定三七提取液中三七皂苷R2(S)、七叶胆苷XVII及人参皂苷F2含量的高效液相色谱法。方法 色谱柱为Waters Acquity UPLC CSH-C18(50 mm×2.1 mm,1.7 μm),流动相为0.01%甲酸-水(A)和0.01%甲酸-乙腈(B),梯度洗脱,检测波长为203 nm,进样量为5 μL,流速为0.35 mL·min-1,柱温为40 ℃。结果 在43 min内可完成三七皂苷R2(S)、七叶胆苷XVII和人参皂苷F2的分离测定。3种皂苷峰面积和浓度线性关系良好(r2>0.999 8);日内和日间精密度RSD<3.4%;回收率为98.4%~102.1%。结论 该方法简便、准确,重复性好,可用于三七提取液中皂苷成分的测定。 相似文献
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目的 考察60Co-γ射线辐照灭菌对三七粉中有效成分三七皂苷R1含量的影响。方法 选择5,8,10 kGy 3种辐照剂量对三七粉进行辐照。采用UPLC测定,比较辐照前后三七粉的需氧菌总数、霉菌和酵母菌总数及其主要有效成分之一三七皂苷R1的含量。结果 三七粉经过5 kGy剂量辐照后,已能达到卫生学标准要求,且有效成分三七皂苷R1含量较辐照前无明显变化;经过8,10 kGy剂量辐照后,三七粉中所含三七皂苷R1有明显变化。结论 60CO-γ射线辐照灭菌的效果理想,但要慎重选择辐照的剂量。 相似文献
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摘 要 目的: 以犬心肌缺血模型为对象,研究中药三七对犬心肌缺血保护作用谱效关系及药效物质基础。方法: 在建立中药三七液相指纹图谱分析方法的基础上,采用结扎犬冠状动脉致急性心肌缺血的模型,运用双变量相关分析和多元回归分析,将三七提取物药效数据与指纹图谱共有峰的相对峰面积相关联,研究药物治疗心肌缺血作用的物质基础及谱效关系。结果: 研究证实人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1均与药效呈显著正相关,其中人参皂苷Rb1可显著降低血清中乳酸等物质水平的升高,人参皂苷Rg1可显著降低血清FFA水平升高,是中药三七治疗心肌缺血的主要有效成分。结论:通过谱效关系研究获得了药效活性物质,建立了三七药材谱效关系评价的方法,客观反映了药物内在质量,为该类中药的创新研究提供了借鉴。 相似文献
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中药三七对大鼠心肌缺血保护作用的谱效学研究 总被引:3,自引:1,他引:2
目的 以大鼠心肌缺血模型为对象,研究中药三七对大鼠心肌缺血保护作用谱效关系及药效物质基础。方法 在建立中药三七液相指纹图谱分析方法的基础上,研究药物治疗心肌缺血作用的物质基础及谱效关系。结果 人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1是中药三七治疗心肌缺血的主要有效成分。结论 通过谱效关系研究直接获得药效活性物质,建立了三七药材谱效关系评价的新方法,客观反映了药物的内在质量,为该类中药的进一步研究提供了新的思路。 相似文献
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摘 要 目的:建立HPLC梯度法同时测定复方三七维康胶囊中三七皂苷R1、人参皂苷Rb1、Rg13种皂苷的含量。方法: 色谱柱:大连Spherisorb C18分析柱(200 mm×4.6 mm,5 μm);流动相:乙腈-水,梯度洗脱;流速:1.0 ml·min-1;检测波长 203 nm。结果:三七皂苷R1、人参皂苷Rg1和Rb1分别在1.06~18.55 μg(r=0.997 3)、2.02~35.35 μg(r=0.998 2)和2.02~35.35 μg(r=0.998 2)之间线性关系良好。平均回收率三七皂苷R1为100.8%(RSD=1.53%,n=5),人参皂苷 Rg1为98.9%(RSD=1.87%,n=5),人参皂苷Rb1为99.7%(RSD=1.90%,n=5)。结论:HPLC梯度洗脱法能够将多种皂苷很好地分离检测,该方法准确可靠,重复性好,可用于控制其质量。 相似文献
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A series of 6-(N-arylamino)-7-methylthio-5,8-quinolinedione derivatives4a-4l was newly synthesized for the evaluation of antifungal activity. 6-(N-Arylamino)-7-methylthio-5,8-quinolinediones were prepared
by regioselective nucleophilic substitution of 6,7-dichloro-5,8-quinolinediones with arylamines in the presence of Ce3+, and Na2S/dimethylsulfate. The MIC values of4a-4l were determined for antifungal susceptibilityin vitro againstCandida species by agar streak method. The derivatives4a-4l had generally potent antifungal activities against all human pathogenic fungi. Especially they had the most potent activity
againstC. krusei at 12.5≈0.8 μg/ml. Compounds4d, 4g, 4h, 4j and4k had more potent antifungal activities than fluconazole. Compounds4g and4h completely inhibited the fungal growth at 0.8≈6.3 μg/ml against allCandida species, while fluconazole inhibited the growth at 25 μg/ml. The compounds such as4g and4h containing an N-(4-bromo-2-methylphenyl)- or N-(4-bromo-3-methylphenyl)amino substituent exhibited the most potent antifungal
activities. 相似文献
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Antifungal activities of 6-[(N-4-bromophenyl)amino]-7-chloro-5,8-quinolinedione (RCK7) were tested. The MIC values of RCK7
were determined for antifungal suceptibility,in vitro againstAspergillus niger, Cryptococcus neoformans andTrichophyton mentagrophyte by standard agar streak method.In vitro, RCK7 showed more potent antifungal activity than fluconazole and ketoconazole. Also, RCK7 was tested forin vivo antifungal activity in the treatment of systemic infection withCandida albicans in normal mice. The therapeutic potential of RCK7 had been assessed by evaluating their survival rate against systemic infections
compared with that of ketoconazole. ED50 of intraperitoneally administered RCK7 was 2.05±0.30 mg/kg but that of ketoconazole was 8.00±0.73 mg/kg, respectively. When
RCK7 was administered intravenously at the ED50 (2.05 mg/kg), the colony counts ofCandida albicans in the liver after 7 days and 14 days were reduced as likely as ketoconazole at the ED50 (8.00 mg/kg), and the better survival rates than ketoconazole’s were achieved after 14 days. The results suggest that RCK7
may be a potent antifungal agent. 相似文献
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目的 对制川乌配方颗粒与标准煎剂抗炎药效等效性进行研究。方法 应用二甲苯致小鼠耳肿胀法比较制川乌标准煎剂及配方颗粒对其抑制作用。结果 在抗炎药效等效性研究中,在小鼠耳廓肿胀抑制率22.51%~38.65%共同效应范围内,1/2、1、2倍临床等效剂量(195.00、390.00、780.00 mg/kg)制川乌标准煎剂等效于0.07、0.20、0.46倍临床等效剂量(27.36、77.67、178.29 mg/kg)制川乌配方颗粒所产生的抗炎效应,制川乌配方颗粒等效剂量DEE制川乌配方颗粒=-22.950+0.258× DEE制川乌标准煎剂(R2=0.958,r=0.979,P<0.01),回归系数b0=-22.950,95% CI为(4.549,41.351),与总体均值0相比,差异有显著性(P<0.05),常数项b1=0.258,95% CI为(-0.218,0.299),与总体均值1相比,差异有显著性(P<0.05)。制川乌配方颗粒与制川乌标准煎剂药剂量在1/8~1倍临床等效应剂量范围内(48.75~390.00 mg/kg)具有可比性,制川乌配方颗粒的等剂量效应EED制川乌配方颗粒=2.963+1.573×EED制川乌标准煎剂(R2=0.923,r=0.961,P<0.01),回归系数b0=2.963,95% CI为(-5.373,11.300),与总体0比较差异有显著性(P<0.05),常数项b1=1.573,95% CI为(1.230,1.915),与总体均值1相比差异有显著性(P<0.05)。结论 在抗炎方面制川乌配方颗粒和制川乌标准煎剂的等效应剂量不具有等效性,制川乌配方颗粒等效应剂量较制川乌标准煎剂小。在抗炎方面制川乌配方颗粒和制川乌标准煎剂的等剂量效应不具有等效性,制川乌配方颗粒等剂量效应较制川乌标准煎剂大。 相似文献
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Synthesis of 35 new compounds of α-chloro-β-(5-nitro-2-furyl) acrylamides and 5-[α-chloro-β-(5-nitro-2-furyl ) vinyl]-oxadiazoles by known methods are reported. In preliminary test in mice 10 compounds were found to possess pronounced activity against Schistosomiasis japonica. Among these Ⅰ12, Ⅰ13, Ⅰ14, Ⅰ20, Ⅱ1 and Ⅱ8 were shown to be the most effective. 相似文献
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C. L. Fortes-Dias M. L. D. Jannotti F. J. L. Franco A. Magalhes C. R. Diniz 《Toxicon》1999,37(12):172
A phospholipase A2 inhibitor has been previously purified and cloned from the blood plasma of the South American rattlesnake, Crotalus durissus terrificus. This inhibitor, named CNF for Crotalus neutralizing factor, interacts with crotoxin, the main neurotoxin from C. d. terrificus venom, abolishing its phospholipase A2 activity. Crotoxin is a heterodimer of an acidic subunit (CA) and a basic phospholipase A2 (CB). CNF acts by forming a stable non-toxic complex with CB, replacing CA in the toxic CA–CB of crotoxin.In the present investigation, we have shown that CNF has a broader specificity. It is able to inhibit the PLA2 activity of the whole venom from the bushmaster snake (Lachesis muta muta), a species evolutionary related to Crotalus. Inhibition experiments have been carried out with four PLA2 active components isolated from L. m. muta venom, one basic and three acidic ones. CNF inhibition is not restricted to the basic PLA2, but extended to the three acidic forms as well. 相似文献
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Toshihiro Akihisa Satoshi Kamo Taketo Uchiyama Hiroyuki Akazawa Norihiro Banno Yosuke Taguchi Ken Yasukawa 《Journal of natural medicines》2006,60(4):331-333
Three triterpene acids, ursolic acid (1), corosolic acid (2), and oleanolic acid (7), were isolated from the ethanol extract of leaves of Perilla frutescens (L.) Britton var. japonica Hara (Labiatae; Japanese name: Egoma). These acids are the active principles responsible for the cytotoxicity against three
cultured human tumor cell lines, HL-60 (leukemia carcinoma), MCF-7 (breast carcinoma), and Hep-G2 (hepatic carcinoma), with
half maximal inhibitory concentration (IC50) values of 12–48 μM. 相似文献
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狭叶藜芦中活性成分的化学研究 Ⅲ.β1-查茄碱的结构和狭叶藜芦碱丙、碱丁的部分结构研究 总被引:2,自引:0,他引:2
自狭叶藜芦中首次分离出β1-查茄碱和两个新碱狭叶藜芦碱丙、碱丁。根据光谱数据和衍生物的制备,测定了β1-查茄碱的结构,初步推定了狭叶藜芦碱丙和碱丁的部分结构。 相似文献