红斑、丘疹及鳞屑性皮肤病

２００００２６９ 寻常型银屑病患者血清补体及Ｋ＋、Ｎａ＋、Ｃｌ－、Ｃａ２＋水平的初步检测／蔡秀玲（中国医科院、中国协和医大皮肤病医院）…／／岭南皮肤性病科杂志．－１９９９，６（２）．－１～３ 检测７１例，其中斑块状（ＰＰｓ）１９例，点滴状（ＧＰｓ）１９例，斑块与点滴状并存者１２例，寻常型（ＰＶ）但未详细分类的２１例。正常人（ＮＣ）２０例。结果：血清总补体和Ｃ３水平ＰＰｓ与ＧＰｓ间、ＰＰｓ和ＧＰｓ与ＮＣ间均无统计学差异（Ｐ＞０．０５），但ＰＰｓ、ＧＰｓ、ＮＣ与ＰＰｓ＋ＧＰｓ间有显著性差异（Ｐ＜０．０…     

用套式PCR法诊断系统性念珠菌病时血清优于全血

用ＰＣＲ法诊断系统性念珠菌病既可以使用血清 ,也可以使用全血 ,但比较这两种类型样本检测的结果还未见正式报道。作者设计了含有 5对特异性内引物的嵌套式ＰＣＲ方法 ,可以扩增白念珠菌、热带念珠菌、假热带念珠菌、克鲁斯念珠菌和光滑念珠菌的核糖体ＲＮＡ(ｒＲＮＡ)的特异性靶序列。真菌特异性通用引物为 :ＩＴＳ1 :ＴＣＣＧＴＡＧＧＴＧＡＡＣＣＴＧＣＧＧ ;ＩＴＳ4:ＴＣＣＴＣＣＧＣＴＴＡＴＴＧＡＴＡＴＧＣ。白念珠菌的内引物为 :ＣＡＬ1 :ＡＡＣＴＴＧＣＴＴＴＧＧＣＧＧＴＧＧＧＣ ;ＣＡＬ3 :ＴＧＧＡＣＧＴＴＡＣＣＧＣＣＧＣＡ…     

疱液在诊断表皮下自身免疫性大疱病中的意义

目的：探讨表皮下自身免疫性大疱病疱液中抗基底膜带（ＢＭＺ）自身抗体的情况及在诊断中的意义。方法：应用非盐裂皮肤及盐裂皮肤间接免疫荧光技术（ＩＦ）检测３８例表皮下自身免疫性大疱病患者疱液和血清中ＩｇＧ、ＩｇＡ、ＩｇＭ、ＩｇＥ及ＩｇＧ亚型ＩｇＧ１～ＩｇＧ４抗ＢＭＺ抗体及滴度，并检测疱液和血清中结合补体Ｃ３的特异性抗ＢＭＺ抗体。结果：（１）疱液和血清中抗ＢＭＺ抗体及滴度和结合补体Ｃ３的特异性抗ＢＭＺ抗体均无显著性差异。（２）大疱性类天疱疮患者的疱液和血清中ＩｇＧ抗ＢＭＺ抗体的亚型分布相同，主要为ＩｇＧ１和ＩｇＧ４。结论：疱液可作为ＩＩＦ检测表皮下大疱病抗ＢＭＺ抗体的另一个有价值的标本来源。     

女性黄褐斑患者的血清酶学及血液流变学初步分析

为了了解黄褐斑与氧自由基，血液流变学的关系，本研究对４８例女性黄褐斑患者和４０例正常对照组血清过氧化脂质（ＬＰＯ）水平，红细胞超氧化歧化酶（ＳＯＤ）活性，全血谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）过氧化氢酶（ＣＡＴ）活性以及血液流变学进行了检测。发现病例组全血ＣＡＴ活性低于对照组（Ｐ〈０．０１），血清ＬＰＯ水平，红细胞ＳＯＤ活性，全血ＧＳＨ－Ｐｘ活性与对照组无显著性差异（Ｐ〉０．０５），日晒对血清酶学     

SDS—PAGE的标化及麻风病人血清谱的初步研究

作者建立了标化的分析血清抗原的ＳＤＳ－ＰＡＧＥ法，并初步分析了麻风，结核，类风湿关节炎病人的血清抗原谱带，发现１２．５％为最适分析胶浓度，在受试血清中同病的不同个体之间条带基本相同，不同病种与正常血清比较有条带减弱甚至缺失的现象，特别是多菌型风血清Ｃ、Ｅ、Ｉ带缺失，少菌型的Ｃ、Ｅ带缺失，提示其对麻风的诊断，分型可能有一定作用，值得扩大样本进一步研究。     

抗心磷脂抗体在银屑病表皮细胞异常增殖中作用机理探讨—附4…

测定了４６例寻常型银屑病患者血清抗心磷脂抗体（ＡＣＡ），以及血浆６－酮－前列腺素Ｆ１α（６－Ｋ－ＰＧＦ１α）和血栓烷（ＴＸＢ２），发现患者循环ＡＣＡ、６－Ｋ－ＰＧＦ１α及ＴＸＢ２水平低下，与皮炎－湿疹组及正常人比较差异非常显著（Ｐ＜０．０１）。相关回归分析显示患者的ＡＣＡ－ＩｇＧ与６－Ｋ－ＰＧＦ１α有一定的相关性（γ＝０．２９０，Ｐ≤０．０５）。对它们之间的关系及其在表皮细胞异常增殖中的作用进行了     

SACT的建立及其与PGL—I—ELISA的比较研究

用抗麻风菌特异酚糖脂Ｉ（ＰＧＬ－Ｉ）单克隆抗体Ｂ８Ｆ１ＩＶ建立了间接血清抗体竞争试验（ｓｃｒｕｍ ａｎｔｉｂｏｄｙ ｃｏｍｐｅｔｉｔｉｏｎ ｔｅｓｔ，ＳＡＣＴ），其性为９９％特异性为９６．５％。以本法与标准化的Ｃ ＰＧＬ－抗原的间接酶联免疫吸附试验（ＰＧＬ－Ｉ－ＥＬＩＳＡ）作了比较。所用麻风血清为１００例（ＬＬ２０、ＢＩ２０，ＢＢ２０，ＢＴ２０），正常人血清为２８例，结果显示丙地在检测ＭＢ病人上无     

皮肌炎、硬皮病及桥本氏甲状腺炎重叠综合征1例

患者女，２１岁。乏力、肌痛、间断发热，并有皮肤、肌肉萎缩变硬２年。皮肤科检查示肌肉萎缩，皮肤弹性差，Ｒａｙｎａｕｄ（＋）。实验室检查：抗Ｓｃｌ－７０（＋），抗Ｊｏ－ｌ（＋），补体Ｃ３、Ｃ４降低，ＣＫ明显高于正常值，Ｔ３降低，ＴＭＡ（＋），ＴＧＡ（＋）。心电图示Ｔ波改变。肺Ｘ线摄片不除外肺间质改变。肌电图为肌原性萎缩相。皮肤、肌肉病理活检符合硬皮病、皮肌炎改变。     

CyclinE和CDK2 mRNA及蛋白质在银屑病皮损表皮中的表达

在哺乳类细胞增生周期中，ＣｙｃｌｉｎＥ于Ｇ１晚期开始与ＣＤＫ２（ｃｙｃｌｉｎｄｅｐｅｎｄｅｎｔｋｉｎａｓｅ２）结合，在Ｇ１／Ｓ转换处形成激酶复合体，对Ｇ１／Ｓ转换起重要调控作用。近年来国外一些学者报道，过表达的ＣｙｃｌｉｎＥ可加速Ｇ１期的进行［１，２...     

沙眼衣原体泌尿生殖道感染265例临床分析

沙眼衣原体泌尿生殖道感染２６５例临床分析祖瑜，常德华国内有关沙眼衣原体泌尿生殖道感染（ＣＵＧ）的报道渐多，但缺乏系统的临床资料。１９９４年３月至１９９５年１月，本所性病门诊确诊ＳＴＤ者１２４１例，其中ＣＵＧ２６５例（２１．４％），现分析如下。ＣＵＧ的...     

New fillers for the new man

ABSTRACT:  Two new collagen-based lidocaine-containing dermal fillers, ArteSense™/ArteFill™ (Artes Medical, San Diego, CA) and Evolence® (Colbar LifeScience Ltd., Herzliya, Israel), have proved to be of particular interest to men, many of whom seek a long-lasting or permanent correction. ArteFill™ has been available in the United States since 2006, and it is expected that Evolence® will reach the American market in 2008. The properties of the two products will be described, and experience based on the administration of many hundreds of syringes of both products by a Canadian dermatologist will be detailed here, with tips and precautions to optimize patient outcomes.     

Outcomes and adverse effects of ablative vs nonablative lasers for skin resurfacing: A systematic review of 1093 patients

It is generally believed that ablative laser therapies result in prolonged healing and greater adverse events when compared with nonablative lasers for skin resurfacing. To evaluate the efficacy of ablative laser use for skin resurfacing and adverse events as a consequence of treatment in comparison to other modalities, a PRISMA‐compliant systematic review (Systematic Review Registration Number: 204016) of twelve electronic databases was conducted for the terms “ablative laser” and “skin resurfacing” from March 2002 until July 2020. Studies included meta‐analyses, randomized control trials, cohort studies, and case reports to facilitate evaluation of the data. All articles were evaluated for bias. The search strategy produced 34 studies. Of 1093 patients included in the studies of interest, adverse events were reported in a total of 106 patients (9.7%). Higher rates of adverse events were described in nonablative therapies (12.2% ± 2.19%, 31 events) when compared with ablative therapy (8.28% ± 2.46%, 81 events). 147 patients (13.4%) reported no side effects, 68 (6.22%) reported expected, transient self‐resolving events, and five (0.046%) presented with hypertrophic scarring. Excluding transient events, ablative lasers had fewer complications overall when compared with nonablative lasers (2.56% ± 2.19% vs 7.48% ± 3.29%). This systematic review suggests ablative laser use for skin resurfacing is a safe and effective modality to treat a range of pathologies from photodamage and acne scars to hidradenitis suppurativa and posttraumatic scarring from basal cell carcinoma excision. Further studies are needed, but these results suggest that ablative lasers are a superior, safe, and effective modality to treat damaged skin.     

Genetic alterations in RAS‐regulated pathway in acral lentiginous melanoma

Studies integrating clinicopathological and genetic features have revealed distinct patterns of genomic aberrations in Melanoma. Distributions of BRAF or NRAS mutations and gains of several oncogenes differ among melanoma subgroups, while 9p21 deletions are found in all melanoma subtypes. In the study, status of genes involved in cell cycle progression and apoptosis was evaluated in a panel of 17 frozen primary acral melanomas. NRAS mutations were found in 17% of the tumors. In contrast, BRAF mutations were not found. Gains of AURKA gene (20q13.3) were detected in 37.5% of samples, gains of CCND1 gene (11q13) or TERT gene (5p15.33) in 31.2% and gains of NRAS gene (1p13.2) in 25%. Alterations in 9p21 were identified in 69% of tumors. Gains of 11q13 and 20q13 were mutually exclusive, and 1p13.2 gain was associated with 5p15.33. Our findings showed that alterations in RAS‐related pathways are present in 87.5% of acral lentiginous melanomas.     

Self‐resolving superficial primary cutaneous mucormycosis in a 7‐week‐old infant

A 7‐week‐old girl, born at 30 weeks' gestational age, presented to clinic for evaluation of a crop of vesicular lesions that were noted after removal of a bandage that had been in place for 4 days. A punch biopsy of the lesion revealed fungal elements that were later identified as Rhizopus spp. The lesion began to self‐resolve, and no further treatment was needed, with full resolution of the lesion by 1 month after presentation. Clinicians should be aware of the variable presentations of mucormycosis and consider fungal infection in the differential diagnosis when evaluating vulnerable patients with skin eruptions.     

Pathogenic role of IL-17 in psoriasis and psoriatic arthritis

Psoriasis is a chronic inflammatory skin disorder resulting from a complex network of cytokines and chemokines produced by various immune cell types and tissue cells. Emerging evidence suggests a central role of IL-17 and IL-23/T17 axis in the pathogenesis of psoriasis, giving a rationale for using IL-17-blocking agents as therapeutics.Three agents targeting IL-17 signaling are being studied in Phase III clinical trials: secukinumab and ixekizumab (IL-17 neutralizing agents), and brodalumab (IL-17 receptor antagonist). Preliminary results are highly promising for all anti-IL17 agents, creating fair expectations on this class of agents as the new effective therapeutic approach for the treatment of psoriasis.