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李卫东 《针灸推拿医学(英文版)》2009,7(2):91-93
目的:观察神经傍刺治疗枕神经痛的疗效。方法:将82例枕神经痛患者随机分为两组。治疗组46例,采用神经傍刺法,取上天柱、天牖穴;对照组36例,采用一般针刺法,取天柱、风池、百会、失眠II、完骨、合谷等。治疗3~5次为1疗程。最多治疗3个疗程,观察疗效。结果:近期总有效率治疗组为93.5%,对照组为69.4%,两组比较差异有统计学意义(P〈0.01)。远期治愈率治疗组为64.1%,对照组为43.3%,两组比较差异有统计学意义(P〈0.05)。结论:神经傍刺法治疗枕神经痛疗效优干一般针刺法。 相似文献
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《中医科学杂志(英文)》2020,7(2):171-180
ObjectiveTo investigate the cardioprotective effect of Yiqi Huoxue granule (YQHXG) in the regulation of autophagy in rats induced with myocardial infarction (MI).MethodsAn acute MI animal model was established by ligation of the left anterior descending branch of the coronary artery in Sprague-Dawley rats. Besides, 20 rats received sham operation were classified into a control group. The remaining 59 rats were randomly divided into MI model group (n = 19), YQHXG group (n = 20), and perindopril group (n = 20). Relevant indicators on days 7 and 28 were observed in each group. Left ventricular function was determined by echocardiography. The structure and morphology of mitochondria, and the number of autophagic vesicles, were observed by transmission electron microscopy. The mRNA and protein expression levels of LC3, FUNDC1, Beclin-1, and BNIP3 were examined in the tissue of the MI marginal area.ResultsCompared with the MI model group, YQHXG showed obvious improvements in cardiac functions. Observing the microscopic morphology of the heart tissue, myocardial tissue damage attenuated, autophagic signs of autophagosomes and autolysosomes reduced, vacuolization in mitochondria mitigated, and mitochondria arranged in order. YQHXG could reduce the degree of tissue lesion after MI and regulate the expression of autophagy-related molecules at different stages. On Day 7, YQHXG significantly downregulated the expression of Fundc1, Becn1, Bnip3 mRNA and reduced the levels of FUNDC1, Beclin-1, BNIP3, and LC3 B proteins expression (all P < .001). On Day 28, YQHXG could upregulate the expression of Becn1, Fundc1 and Bnip3 mRNA and increased the levels of the corresponding proteins expression (all P < .001). Besides, it also increased LC3 B protein expression level (P = .0344).ConclusionYQHXG regulated the expression of mitochondrial autophagy-related factors in myocardial tissue and mitochondrial autophagic activity at different stages to protect the heart following MI. 相似文献
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目的观察头穴丛刺方法对慢性脑缺血大鼠认知功能和VEGF表达的影响。方法 40只大鼠采用改良的双侧颈总动脉永久性结扎法(2VO)制备慢性脑缺血模型,造模成功的大鼠分为假手术组(A组)、模型组(B组)和头穴丛刺组(C组)和尼莫地平组(D组)。C组采用头穴丛刺法,D组行尼莫地平灌胃,A组和B组不予治疗。治疗结束后采用Morris水迷宫检测大鼠的认知功能;光镜下观察海马细胞形态变化;免疫组化检测海马VEGF蛋白表达。结果术后8星期B、C、D组逃避潜伏期明显延长,与A组相比差异具有统计学意义(P<0.05);C组和D组与B组相比,逃避潜伏期缩短(P<0.05)。HE染色C组和D组变性、死亡神经细胞较B组明显减轻。免疫组化A组偶见VEGF阳性细胞表达,B、C、D组均可见VEGF蛋白表达增高,与A组比较差异具有统计学意义(P<0.05);C组和D组较B组表达升高(P<0.05)。结论头穴丛刺能够明显改善大鼠的认知功能,其可能通过上调VEGF蛋白表达,从而对慢性脑缺血后新生血管的形成具有促进作用,对神经元恢复具有重要的意义。 相似文献
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[目的] 探讨运用头针丛刺方法针刺焦氏头穴运动区下点治疗顽固性特发性面神经麻痹的临床疗效。[方法] 将56例患者随机分为两组, 治疗组采用头针丛刺方法针刺焦氏头穴运动区下点, 对照组采用常规针刺方法针刺面部穴位, 针刺1个月后观察临床疗效。[结果] 两组患者治疗后与治疗前相比差异均有统计学意义(P<0.05), 治疗后组间比较差异有统计学意义(P<0.05), 治疗组优于对照组。治疗组总有效率为96.4%, 对照组总有效率为85.7%.[结论] 运用头针丛刺方法针刺焦氏头穴运动区下点, 治疗顽固性面瘫疗效确切优于传统针刺方法。 相似文献
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Clinical Study on Acupuncture in Treating Diarrhea-predominant Irritable Bowel Syndrome 总被引:1,自引:0,他引:1
目的:观察腹泻型肠易激综合征(irritable bowel syndrome,IBS)患者结肠粘膜5.HT变化及隔药灸治疗的临床疗效,初步探讨其作用机理。方法:-临床收集腹泻型IBS患者73例,随机分为隔药灸组(37例)和针刺组(36例)进行治疗,观察临床疗效,并观察隔药灸治疗对典型患者结肠粘膜5-HT表达的影响。结果:隔药灸组痊愈率为43.2%,针刺组痊愈率为36.1%,两组间无统计学差异(P〉0.05);隔药灸治疗能够改善患者结肠粘膜5-HT异常增高的表达。结论:隔药灸能够改善腹泻型IBSIIg床症状,可能是通过调节5-HT表达发挥治疗作用。 相似文献
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目的:头穴电刺激脑梗死大鼠不同时间窗的疗效观察。方法:将健康雌性大鼠分为模型对照组、假手术对照组及头穴电刺激组,每组又分为治疗1天、3天、7天、14天4个亚组,每组15只。头穴电刺激组、模型对照组采用线栓法制备大脑中动脉急性脑梗死大鼠模型,假手术对照组只分离动脉不结扎插线。对3组大鼠Zea Longa评分,造模24 h内对头穴电刺激组大鼠针刺大椎、百会进行电刺激治疗,每日1次,每次30 min,治疗1天、3天、7天、14天,其余两组于每天相同时间将大鼠固定于实验台上,不予处置。在治疗后1天、3天、7天、14天对各组大鼠进行评分,断头取脑,用PT-PCR方法检测Caspase-3mRNA的表达变化。采用SPSS统计软件进行统计分析。结果:头穴电刺激治疗14天组和治疗7天组Zea Longa评分疗效显著(P〈0.05);头穴电刺激组中治疗14天组Caspase-3 mRNA的表达量明显低于治疗1天、3天(P〈0.05)。结论:对急性脑缺血性脑梗死大鼠行头穴电刺激治疗,可促进其肢体功能的恢复,14天疗程对大鼠肢体功能的恢复疗效最佳。 相似文献
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目的观察天麻素对戊四氮诱导癫痫模型大鼠p-AKT和caspase 3的影响。方法将69只Wistar大鼠分为空白对照组18只、癫痫模型组28只(PTZ组,pentylenetetrazole,55~75 mg/kg)、天麻素干预组23只(Gas组,Gastrodin,200 mg/kg),除空白对照组外均腹腔注射戊四氮(PTZ)诱导形成癫痫模型,天麻素干预组给予天麻素(Gas)干预。电镜下观察各组大鼠海马组织形态学变化,免疫组化及Western blot检测各组大鼠海马组织丝氨酸/苏氨酸蛋白激酶(p-AKT)、含半胱氨酸的天冬氨酸蛋白水解酶(caspase 3)蛋白的表达。结果与对照组比较,PTZ组海马区表现为明显的癫痫病理改变,神经元变性及坏死显著,主要为大锥体细胞和部分齿状回颗粒细胞,损伤细胞细胞核固缩,核碎裂样改变,胞浆肿胀,尼氏体消失,细胞周隙增大,体积增大。药物干预后病理改变改善。大鼠癫痫发作后,癫痫模型组p-AKT、caspase 3蛋白表达高于空白对照组(P0.05)。与癫痫模型组比较,天麻素干预组caspase 3的表达下调(P0.05),在第3天差异尤为显著(P0.01)。结论天麻素通过减少p-AKT、caspase 3蛋白表达,起到抗凋亡的作用,对癫痫模型起到一定保护作用。 相似文献
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ObjectiveTo observe the differences in the therapeutic effect on refractory facial paralysis between meridian sinew needling therapy and routine penetrating needling therapy.MethodsA total of 46 patients with refractory facial paralysis were randomly divided into a meridian sinew needling group and a routine penetrating needling group, 23 cases in each one. The same acupoints were selected in the two groups. Besides using routine acupuncture technique, a part of acupoints were stimulated with the meridian sinew needling technique in the meridian sinew needling group. In the routine penetrating needling group, a part of acupoints were stimulated with the routine penetrating needling technique. In both of the groups, the needles were retained for 30 min and the treatment was given once every two days, 10 treatments made one course. A total of 3 courses of treatment were required at the interval of 2 days. The score and grade of facial nerve function were recorded before and after treatment in the patients of the two groups. The clinical therapeutic effect was evaluated.ResultsAfter treatment, the scores of facial nerve function were significantly improved compared with that before treatment in the patients of the two groups (both P < 0.05). The difference value of the score of facial nerve function before and after treatment in the meridian sinew needling group was higher than that in the routine penetrating needling group, indicating a statistical significance (P < 0.05). The grades of facial nerve function after treatment were different significantly as compared with those before treatment in the patients of the two groups (both P < 0.05). However, the difference was not significant between the two groups after treatment (P > 0.05). After treatment, the total effective rate in the meridian sinew needling group was 100%, which was higher than 91.3% in the routine penetrating needling group, while without statistical significant difference (P > 0.05).ConclusionMeridian sinew needling therapy of acupuncture greatly improves facial nerve function as compared with routine penetrating needling therapy. It would be an potential effective acupuncture technique for refractory facial paralysis. 相似文献
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电针诱导大鼠脑缺血耐受作用的穴位特异性研究 总被引:17,自引:3,他引:17
目的:探讨电针诱导大鼠脑缺血耐受作用的穴位特异性。方法:40只雄性SD大鼠,随机分为4组,每组各10只,即空白组、戊巴比妥组、针刺肢体组和针刺“百会”组。最后一次处理24h后采用颈内动脉尼龙线线栓法致右大脑中动脉栓塞(120min)模型,观察再灌注后24h时神经功能损害并取大脑行TTC染色以测量脑梗死容积。结果:再灌注24h时神经功能损害评分及脑梗死容积。针刺“百会”组均明显小于其余3组(P均<0.05),针刺肢体组与两对照组无明显差异(P>0.05)。结论:电针“百会”预处理减轻大鼠神经功能损害程度明显优于针刺肢体。 相似文献
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目的:探析头穴丛刺法联合川芎嗪对大鼠脑梗死后神经元突触重建的影响。方法:行简单随机法将72只SD大鼠分组,备制大脑中动脉闭塞(MCAO)脑梗死模型,A组18只(MCAO模型对照),B组18只(头穴丛刺法),C组18只(川芎嗪),D组18只(头穴丛刺法+川芎嗪)。B组、D组取百会及穴位邻近左侧与右侧2 mm处针刺;C组、D组以盐酸川芎嗪注射液20 mg/(kg·d);A组以同剂量0.9%氯化钠溶液注射。于MCAO建模7、14、28 d比较各组星形胶质细胞、突触个数,检测突触素、GFAP的含量。结果:随时间延长,各组神经元突触、星形胶质细胞个数均有增长,突触素、GFAP表达均有提高,其中B、C、D组增长明显(P<0.05)。14 d、28 d D组神经元突触、星形胶质细胞个数及突触素、GFAP表达均显著高于A、B、C组,差异有统计学意义(P<0.05),其次B、C组14 d、28 d显著高于A组(P<0.05),B、C两组7、14、28 d均无统计学意义(P>0.05)。结论:川芎嗪联合头穴丛刺可促进MCAO脑梗死大鼠神经元突触增殖,增强突触素、GFAP表达,提高突... 相似文献
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ObjectiveTo observe the effects of the thumb-tack needling therapy on knee joint pain and joint function in the elderly patients with knee osteoarthritis (KOA) and compared with medication.MethodsA total of 60 patients were randomized into a thumb-tack needling group and a medication group, 30 cases in each one. In the thumb-tack needling group, the therapy with the thumb-tack needle retaining and waiting for qi was adopted. The acupoints were Hèdĭng (EX-LE2鹤顶), Nèixīyăn (内膝眼EX-LE4), Dúbí (犊鼻ST35), Xuèhăi (血海SP10), Liángqiū (梁丘ST34) and Ashi points on the affected side. The needles were retained for 24 h. This therapy was given once every two days, 3 times a week. The treatment for 2 weeks was as one course and consecutive 2 courses of treatment were required. In the medication group, diclofenac sodium sustained release tablets were taken orally, 75 mg each time, once daily, consecutively for 4 weeks. Successively, the score of visual analogue scale (VAS) at the affected knee before treatment, after treatment and 3 months after treatment as well as Lequesne indicator before and after treatment were evaluated. The clinical therapeutic effect was observed in the patients of the two groups after treatment.Results(1) After treatment, VAS scores were reduced as compared with those before treatment in the patients of the two groups (both P < 0.05). After treatment, VAS score in the thumb-tack needling group was lower than that in the medication group (P < 0.05). (2) After treatment, the scores of all the items of Lequesne indices reduced as compared with those before treatment in the patients of the both groups, indicating significance differences (all P < 0.05). After treatment, compared with the medication group, the score of pain, morning stiffness and walking ability as well as the total score were all lower in the thumb-tack needling group, indicating significant differences (all P < 0.05). (3) The curative and remarkably effective rate and the total effective rate were 66.7% and 93.3% in the thumb-tack needling group, higher than 56.7% and 86.7% in the medication group respectively (both P < 0.05).ConclusionThe thumb-tack needling therapy effectively relieves knee joint pain and improves joint function in the patients and the therapeutic effect is better than medication group. Hence, this therapy deserves to be promoted in clinical practice. 相似文献
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Jiayi Ma Shuxian Sun Cheng Ni Lingru Li Jing Xia Houqin Li Huirong Song Xujun Heng Dandan Hu Yuanyuan Li 《中医科学杂志(英文)》2021,8(3):224-237
ObjectiveTo investigate the proteomic characteristics of overweight/obesity and related abnormal glucose and lipid metabolism caused by phlegm-dampness retention to identify related biomarkers.MethodsSeventy-one subjects were enrolled in the study. We assessed blood glucose, blood lipids, body mass index (BMI), and phlegm-dampness pattern, which was confirmed by a traditional Chinese medicine clinician. Of the participants, we included healthy participants with normal weight (NW, n = 23), overweight/obese participants with normal metabolism (ONM, n = 19), overweight/obese participants with pre-diabetes (OPD, n = 12), and overweight/obese participants with marginally-elevated blood lipids (OML, n = 17). Among them, the ONM, OPD, and OML groups were diagnosed with phlegm-dampness pattern. The data-independent acquisition (DIA) method was first used to analyze the plasma protein expression of each group, and the relevant differential proteins of each group were screened. The co-expressed proteins were evaluated by Venn analysis. The pathway analyses of the differential proteins were analyzed using Ingenuity Pathway Analysis (IPA) software. Parallel reaction monitoring (PRM) was used to verify the differential and common proteins in each group.ResultsAfter comparing ONM, OPD, and OML groups with NW group, we identified the differentially expressed proteins (DEPs). Next, we determined the DEPs among OPD, OML, and ONM groups. Using Venn analysis of the DEPs in each group, 24 co-expressed proteins were screened. Two co-expressed proteins were verified by PRM. IPA analysis showed that pathways including LXR/RXR activation, acute phase response signaling, and FXR/RXR activation were common to all three groups of phlegm-damp overweight/obesity participants. However, the activation or inhibition of these pathways was different among the three groups.ConclusionParticipants with overweight/obesity have similar proteomic characteristics, though each type shows specific proteomic characteristics. Two co-expressed proteins, VTN and ORM1, are potential biomarkers for glucose and lipid metabolism diseases with overweight/obesity caused by phlegm-dampness retention. 相似文献
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[目的]观察刺络放血联合艾灸对实验性慢性肾功能衰竭大鼠肾功能的影响。[方法]将54只大鼠随机分为3组:正常组、模型组、刺络放血联合艾灸治疗组(简称刺灸组)。采用腺嘌呤溶液灌胃复制慢性肾功能衰竭大鼠模型,刺灸组自造模之日起予刺络放血联合艾灸治疗21d,余动物不予任何治疗措施。以肌酐、尿素氮和肾组织的病理学变化为观察指标。[结果]21d后,模型组大鼠存活率45.5%,刺灸组大鼠存活率81.8%。模型组大鼠肌酐、尿素氮较正常组均明显上升,具有显著性差异(P0.01),刺灸组尿素氮较模型组有下降趋势,肌酐较模型组下降,具有差异(P0.01)光镜下肾脏组织病理提示模型组肾小管明显扩张,肾间质大量炎细胞浸润、肾间质纤维化,肾小管和间质可见黄褐色腺嘌呤结晶。刺灸组亦出现上述病变,病变程度较模型组轻。[结论]刺络放血联合艾灸具有减轻腺嘌呤致慢性肾功能衰竭大鼠肾功能损害和肾脏组织损伤作用的趋势。 相似文献
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Objective: To study the therapeutic effect of treating apoplectic pseudobulbar paralysis with acupuncture.Method: Consciousness-restoring & obstruction-clearing needling technique and points Fengchi (GB 20), Yifeng (TE 17), Wangu (GB
12), Neiguan (PC 6), Shuigou (GV 26), Sanyinjiao (SP 6), Shanglianquan (Ex-HN), Jinjin (Ex-HN 12), Yuye (Ex-HN 13) and Baihui
(GV 20) were selected to treat 300 cases of apoplectic pseudobulbar paralysis.Result: Cure: 189 cases, marked effect: 71 cases, effective: 32 cases, no effect: 8 cases and the whole effective rate is 97.3%.Conclusion: Consciousness-restoring & obstruction-clearing needling technique is effective in treating apoplectic pseudobulbar paralysis.
JIANG Ge-li, MD of acupuncture, senior consultant doctor, professor, engages in the theoretical study of psychosomatic medicine
and clinical treatment method of hyperplastic spine arthrosis and apoplexy sequelae.
Translator: HAN Chou-ping 相似文献
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目的观察青蒿素对糖尿病心肌病大鼠心功能及纤维化的影响。方法 Wistar大鼠随机取正常组8只,其余大鼠以一次性腹腔注射链脲佐菌素造糖尿病模型。16周后,经超声检测糖尿病心肌病造模成功大鼠,再随机分为糖尿病心肌病组、青蒿素治疗组,每组8只。各组分别采取相应干预措施。采用HE染色观察心脏的病理改变,用Masson三色染色法观察胶原纤维表达并测定其表达量,应用超声心动图评测Wistar大鼠的心脏功能。结果与正常组比较,糖尿病心肌病组胶原纤维的表达明显升高(P0.01),E/A、LVEF、FS明显下降(P0.01),心脏收缩舒张功能明显降低。与糖尿病心肌病组比较,青蒿素治疗组大鼠胶原纤维的表达明显降低(P0.01),E/A、LVEF、FS明显上升(P0.01),心脏收缩舒张功能明显改善。结论青蒿素可通过减轻心脏的纤维化来提高大鼠的心功能。 相似文献
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《世界针灸杂志》2022,32(3):223-229
ObjectiveTo analyze the effects of cluster needling at scalp acupoints (CNSA) on behavioral performance and expression of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the hippocampus of rats with schizophrenia, and therefore, to shed light on the mechanism of action of CNSA in attenuating schizophrenia.MethodsThirty-six Wistar rats were randomly divided into the control, model, risperidone, and CNSA groups (9 rats per group). The schizophrenia model was prepared by injecting 0.1 mg/mL dizocilpine maleate (MK-801) for 14 consecutive days. Subsequently, rats in the risperidone and CNSA groups were subjected to the following therapy for 14 consecutive days: (1) Risperidone group: intragastric administration of risperidone suspension (0.4 mg/kg); (2) CNSA group: the “GV 20″ “Qiánd?ng (前顶GV 21) ” “Shéntíng (神庭GV 24) ” “Xìnhuì (囟会 GV 22) ” “Tōngtiān (通天BL 7) ” “Luòquè (络却BL 8) ” “Qūchā (曲差BL 4) ” and “W?chù (五处 BL 5) ” acupoints were selected for needle positioning. Following 14-day intervention period, the Morris water maze experiment and open field experiment were performed. Finally, hippocampal tissue specimens were collected and SOD, CAT, and GSH-Px expression levels were measured by ELISA.Results(1) Morris water maze experiment: Following the 14-day model construction period, the model, risperidone, and CNSA groups showed a significant increase in escape latency (all P < 0.05) and a significant decrease in the number of platform crossings (all P < 0.05) compared with the control group, indicating successful induction of schizophrenia in the rat model. At the end of the intervention period (28d), the risperidone and CNSA groups showed a significant decrease in escape latency (both P < 0.05), and the CNSA group showed a significant increase in the number of platform crossings (P < 0.05) compared with the model group. (2) Open field experiment: At 14d, the model, risperidone and CNSA groups exhibited a significant decrease in the travelled distance and amount of time spent in the central zone (all P < 0.05) compared with the control group (all P < 0.05). At 28d, the risperidone and CNSA groups showed a significant increase in the travelled distance and percentage of time spent in the central zone (P < 0.05 or P < 0.01). (3) Antioxidant enzyme expression: At 28d, the model group exhibited significant decreases in the hippocampal SOD, CAT, and GSH-Px levels, compared with the control group (P < 0.01 or P < 0.001).ConclusionCNSA enabled the attenuation of cognitive impairment and enhancement of memory and learning abilities in the rat model of schizophrenia, plausibly through inhibition of the expression of oxidative stress factors in the hippocampus. 相似文献
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目的:观察头穴丛刺对慢性脑缺血大鼠认知功能障碍及VEGF表达的影响,并探讨其作用机制.方法:采取国内改进的双侧颈总动脉永久性结扎法制备慢性脑缺血大鼠模型,制备成功的大鼠随机分为3组:缺血组、头穴丛刺组、尼莫地平组.Morris水迷宫检测大鼠学习记忆能力,HE染色观察大鼠额叶皮层和海马区的细胞形态变化,免疫组化检查VEGF蛋白表达的情况.结果:与假手术组相比,缺血组大鼠逃避潜伏期明显延长(P<0.05);与缺血组大鼠比较,头穴丛刺组和尼莫地平组大鼠额叶皮层和海马区VEGF蛋白表达明显增多(P<0.05).结论:头穴丛刺法能上调VEGF在慢性脑缺血大鼠中的表达,减轻大鼠的缺血缺氧情况,改善慢性脑缺血大鼠认知功能障碍的状况. 相似文献
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廖恒 《世界科学技术-中医药现代化》2022,24(8):3251-3256
目的 观察“百会、神庭、水沟”穴电针联合跑轮训练对大脑中动脉闭塞(middle cerebral artery occlusion, MCAO)脑缺血再灌注(cerebral ischemiac reperfusion, CIR)损伤模型大鼠神经及运动功能重建的影响。方法 SPF级雄性SD大鼠50只,取10只体重相近的设为假手术组,将另40只采用Longa线栓法制作CIR模型并选取其中造模成功的32只采用随机数字表法均分模型组、电针组、跑轮组和电针+跑轮组,每组8只。电针组选“百会、神庭、水沟”穴电针治疗;跑轮组进行自主跑轮训练;电针+跑轮组治疗同电针组和跑轮组;治疗5次/周,共4周。采用经皮层电刺激记录运动诱发电位(motor evoked potential, MEP)并结合Zea-Longa神经学评分评价神经传导功能;采用悬吊、Grip test评分及腓肠肌张力实验评估肢体肌力和躯体平衡及运动功能的协调性;大鼠血脑屏障通透性采用伊文思蓝渗透法观察。结果 电针组Zea-Longa评分及MEP各时相点潜伏期低于跑轮组,MEP各时相点波幅峰值高于跑轮组(P<0.05)。跑轮组Grip test评分、悬吊时间和等长收缩肌力均高于电针组,MEP各时相点波幅峰值低于电针组(P<0.05)。而电针+跑轮组Grip test评分、悬吊时间、MEP各时相点波幅峰值及等长收缩肌力均高于电针组和跑轮组,Zea-Longa评分及MEP各时相点潜伏期均低于电针组和跑轮组(P<0.05)。结论 电针有利于神经传导功能重建,跑轮训练可促进CIR模型大鼠骨骼肌重塑和运动功能恢复,在防治脑缺血再灌注损伤时二者具有协同作用。 相似文献