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1.
Cocaine and a number of different fractions of a crude ethanol extract of the coca leaf (E. coca) were subjected to a local anesthetic screen using rat tail withdrawal from electric shock. Following an intradermal injection of 0.1 ml of a 2.0% (w.v) solution of cocaine HCl, an immediate response was observed. Two of the coca fractions also produced some local anesthesia. An alkaloidal fraction, containing an equivalent amount of cocaine, produced a maximum effect that was approximately 20% less than that observed with cocaine. The only other fraction producing any effect, a water soluble cocaine-free fraction, showed a maximum response that was approximately 30% of that observed with cocaine. 相似文献
2.
John A. Bedford Carlton E. Turner Hala N. Elsohly 《Pharmacology, biochemistry, and behavior》1982,17(5):1087-1088
The 24 hour lethal effects of cocaine were compared to those of a crude ethanol extract of the coca leaf (Erthroxylon coca) in male, Swiss mice. Various doses of cocaine HCl and coca leaf extracts suspended in a Tween 60, Arlacel 83, and distilled water vehicle were injected IP into groups of 10 mice. The LD50 for cocaine was 95.1 mg/kg. The LD50 for the coca extract was 3450 mg/kg. The LD50 of the extract based on its cocaine content was 31.4 mg/kg. The results clearly indicate that the coca leaf contains constituents other than cocaine that can contribute to a toxic effect of the plant. 相似文献
3.
John A. Bedford Marvin C. Wilson Hala N. Elsohly Craig Elliott Glenda Cottam Carlton E. Turner 《Pharmacology, biochemistry, and behavior》1981,14(5):725-728
The effects of two cocaine free fractions of an ethanol extract of the coca leaf (E. coca) were compared using actometric and limited access food comsumption paradigms in rats. Several intraperitoneal doses of two different fractions were tested in both procedures. Neither of the fractions produced any alteration in locomotor activity. Both fractions did, however, produce reductions in food consumption at two or more of the doses tested. The results clearly demonstrate that the coca leaf contains constituents other than cocaine that are biologically active. 相似文献
4.
John A. Bedford Greg L. Nail Ronald F. Borne Marvin C. Wilson 《Pharmacology, biochemistry, and behavior》1981,14(1):81-83
A discriminative stimulus paradigm was employed to train eight male and female Wistar rats to discriminate 5.0 mg/kg cocaine HCl from 2.0 ml/kg saline. Subjects responded in a two bar operant chamber on an FR 30 schedule for food reinforcement. All sessions followed a 10 minute pretreatment with either saline, the training dose of cocaine, four probe doses of cocaine HCl (1.0, 2.5, 7.5, 10 mg/kg), four probe doses of norcocaine (1.0, 2.5, 5.0, 7.5 mg/kg) or four probe doses of N-allylnorcocaine (5.0, 7.5, 10, 20 mg/kg). All probe doses were tested using an extinction procedure. The three highest doses of cocaine generalized to cocaine while the 1.0 mg/kg dose of cocaine generalized to saline. The two highest doses of norcocaine generalized to cocaine while the 2.5 mg/kg dose of norcocaine resulted in 57% responding on the cocaine lever with the 1.0 mg/kg dose generalizing to saline. Only the highest dose of N-allylnorcocaine was found to generalize to cocaine with the intermediate doses resulting in an intermediate level of responding occurring on the cocaine lever. The 5.0 mg/kg dose of N-allylnorcocaine generalized to saline. 相似文献
5.
George L. Vee Gregory B. Fink George H. Constantine 《Pharmacology, biochemistry, and behavior》1983,18(4):515-517
Dose response curves for reducing limited access food consumption were determined for cocaine HC1 IP, cocaine HC1 PO, and whole Erythroxylum coca extract PO. The ED50's for cocaine HC1 in drug naive rats were 19.6 mg/kg (IP) and 34.6 mg/kg (PO). When the dose of E. coca extract was expressed in terms of cocaine HC1 content, the ED50 was 52.6 mg/kg (PO). When dose response curves were determined in rats that had received cocaine (45 mg/kg, PO) for 30 days, a shift to the right in the cocaine HC1 curve (an ED50 of 98.4 mg/kg PO) indicated tolerance. However, the shift to the right was less for E. coca extract than for cocaine HCI. Although the anorexic activity of E. coca extract was less than that of an equivalent amount of cocaine in naive rats it was often more than that of equivalent doses of cocaine HC1 in tolerant rats. Interaction with other constituents of E. coca extract appears to alter the potency of the cocaine content of the extract in different directions in naive and tolerant rats. 相似文献
6.
《The International journal on drug policy》2014,25(6):1113-1123
Coca is a native bush from the Amazon rainforest from which cocaine is extracted. Growing coca is a profitable activity; however, not all farmers located in the coca-supply areas do so. Little is known about farmers’ motivations for the decision to grow coca and if so, how much to grow. This article evaluates the influence of monetary and non-monetary factors on these decisions. The study is based on a survey of 496 households in an indigenous Aymara community in Peru. The results suggest, for example, that farmers are more likely to cultivate coca when their plots are characterized by flatter agricultural slopes and when in debt. In relation to the scale of coca cultivation, farmers can be classified into two groups. The larger group (73 percent) grows a high number of coca bushes when facing economic hardship; farmers in the second group (27 percent) seem to be more motivated by the potential profits from coca production relative to coffee, the alternative crop in the area. Therefore, the results support the common notion that farmers cultivate coca in accordance with economic need. Nonetheless, non-economic factors also influence the number of coca bushes cultivated and offer an additional opportunity to reduce coca cultivation if explicitly considered in drug-control policies. This research also discusses potential farmers’ responses to different coca-growing reduction strategies. Common drug-control policies such as organic coffee certification, road construction, and education have mixed effects on coca cultivation, depending on the type of coca grower. As such, farmers’ motivations are heterogeneous and the design of effective drug-control policies needs to reflect this. 相似文献
7.
《Substance use & misuse》2013,48(10):1227-1262
Current criticisms of chemical aversion therapy are delineated and their validity assessed. Data pertaining to the effectiveness, acceptability, in-trusiveness, availability of alternative treatments, cost-effectiveness, and theoretical foundations of chemical aversion therapy are examined. It is concluded that available evidence supports the efficacy of chemical aversion therapy with respect to production of conditioned aversion to alcohol and treatment outcome. 相似文献
8.
F C Colpaert C J Niemegeers P A Janssen 《Pharmacology, biochemistry, and behavior》1979,10(4):535-546
The experiments reported here were undertaken to examine the neuropharmacological characteristics of drugs inducing stimulus generalization with cocaine as a cue. Experiment 1 indicated that d-amphetamine (ED50: 0.17 mg/kg), 1-amphetamine (0.45 mg/kg), methylamphetamine (0.15 mg/kg), methylphenidate (0.55 mg/kg) and nomifensine (0.32 mg/kg) induce stimulus generalization with cocaine in rats trained to discriminate 10 mg/kg cocaine from saline; this generalization occurred in 100% of the animals, proceeded along steep slopes (s: 1.27 to 1.88 in log-probit plots), and was not associated with behaviorally toxic effects. Amantadine (57.8 mg/kg; s=1.85), apomorphine (0.33 mg/kg; s=1.77), piribedil (8.4 mg/kg; s=10.6) and bromocryptine (>40 mg/kg) also induced stimulus generalization to some extent, but this generalization was partial in some cases, proceeded along a shallow slope with piribedil, and was invariably associated with severe rate depressant effects. Ten mg/kg, but not 1.25 mg/kg hydroxyamphetamine induced generalization in 3 out of 8 rats. Experiment 2 revealed that tranylcrypromine (2.5 mg/kg; s=1.7), fentanyl (0.068 mg/kg; s=1.34), morphine (>10 mg/kg), phencyclidine (0.81 mg/kg; s=1.43), and benztropine (9.2 mg/kg) induce stimulus generalization with cocaine, whereas lidocaine, procaine, chlordiazepoxide, imipramine, desipramine, mescaline, LSD, isopropamide, and atropine do not. Experiment 3 shows that propranolol (1.25 to 40 mg/kg) and isoproterenol (0.63 to 2.5 mg/kg) induce a biphasic generalization with cocaine. Experiment 4 discloses that rats trained to discriminate 10 mg/kg propranolol from saline generalize their training drug along a linear gradient, but generalize cocaine along a biphasic gradient. It is suggested (a) that stimulus generalization with cocaine may be contingent upon increasing the functional availability of endogenous dopamine and, perhaps, of norepinephrine irrespective of the presynaptic mechanism from which such increase may result and (b) that differential stimulus generalization of drugs with cocaine (in terms of dose, subjects, slope, and rate effects) may parallel their differential primary reinforcing properties. 相似文献
9.
J C Winter 《Pharmacology, biochemistry, and behavior》1985,22(1):15-17
A group of six rats was trained to discriminate the effects of diphenhydramine (10 mg/kg; 30 min pretreatment time) and saline in a two-lever choice task using a fixed ratio schedule of water reinforcement. Stimulus control was assumed to be present when 80% or more of the first ten responses were appropriate for the treatment condition on each of five consecutive days. Diphenhydramine established stimulus control in each of the subjects. The mean number of sessions prior to the onset of criterion performance was 26 (standard error = 7). A second group of six rats was similarly trained with chlorpheniramine (10 mg/kg; 30 min pretreatment time) and saline. Four of the group reached criterion performance in a mean of 56 sessions (SE = 7). The diphenhydramine stimulus generalized completely to promethazine, azatidine, and chlorpheniramine. In rats trained with chlorpheniramine, only promethazine and azatidine substituted completely while diphenhydramine yielded intermediate results, i.e., significantly different from both training conditions. It is concluded that the relative propensity of antihistamines to induce sedation in humans is not correlated with distinctive stimulus properties in the rat. 相似文献
10.
Cocaine-like discriminative stimulus effects of novel cocaine and 3-phenyltropane analogs in the rat
RATIONALE: Cocaine dependence is a major health concern and there are no effective pharmacotherapies currently available. Although cocaine is an indirect DA agonist that binds to all three monoamine transporters, there is much evidence implicating a greater role for the dopamine (DAT) than norepinephrine (NET) and serotonin (SERT) transporters in the behavioral effects of cocaine. As such, several groups have developed compounds that exhibit high affinity and selectivity for the DAT. OBJECTIVE: The present investigation examined the cocaine-like discriminative stimulus effects in rats of novel cocaine analogs (RTI 12, 13, 15) and 3-phenyltropane analogs (RTI 111, 112, 113, 114, 117 120, 121, 123, 134 and 152) of which several exhibit high affinity (e.g., <7 nM) and selectivity for the DAT. RESULTS: During dose-effect testing all drugs produced 75-100% cocaine-lever responding. Analyses indicated that the potency of the compounds to produce cocaine-like discriminative stimulus effects was correlated with their affinity for the DAT and the NET but not SERT. Due to the extremely large concentrations (e.g., 10,000-31,024 nM) needed to occupy the NET in vitro, it is doubtful if the doses administered had meaningful NET activity. The selectivity at the DAT, relative to the other transporters, was not indicative of the potency with which these drugs substituted for cocaine. CONCLUSIONS: The cocaine-like discriminative stimulus of the RTI compounds tested appear to be mediated by the DAT, however the extent to which the NET is involved remains unclear. Additionally, several of the RTI compounds had properties consistent with those thought desirable in a pharmacotherapeutic for cocaine dependence. 相似文献
11.
James L. York 《Psychopharmacology》1978,60(1):19-23
Five different groups of rats were trained in a food-motivated, bar-pressing task to discriminate from the nondrug condition (physiologic saline, i.p.) the effects produced by i.p. injections of ethanol (330, 660, or 990 mg/kg), sodium barbital (80 mg/kg), or sodium phenobarbital (25 mg/kg). The establishment of highly effective discriminations required 20–40 training sessions for all drugs, with the exception that rats trained with 330 mg/kg of ethanol required 80–100 training sessions. After the drug-nondrug discriminations were well established, cross tests revealed that ethanol did not elicit drug-appropriate responding in the groups trained with sodium barbital or sodium in the groups trained with sodium barbital or sodium phenobarbital. However, both barbiturates elicited drug-approppriate responding to some extent in rats trained with ethanol as the discriminative stimulus. With barbital, the greatest generalization was observed in rats trained with the low dose of ethanol (330 mg/kg). The findings emphasize the need for the use of several training doses and for transfer tests in both directions when the stimulus effects of drugs are compared.A preliminary report of part of these findings was presented at the Annual Meeting of the American Society for Pharmacology and Therapeutics, Davis, California, 1975. 相似文献
12.
Rats were trained to discriminate cocaine, 10.0 mg/kg, using a two-lever operant procedure. Dose-effect data were determined for the substitution of cocaine, diethylpropion, methylphenidate, phenmetrazine, phentermine, and fenfluramine for the cocaine stimulus. All of these drugs, except fenfluramine, substituted fully for the cocaine stimulus. Subsequently, training was halted and cocaine, 20 mg/kg/8 h, was administered for 9 days, and dose-effect data were redetermined for all of these drugs on days 7–9 of chronic administration. Chronic administration of cocaine produced tolerance to the stimulus properties of cocaine, and cross-tolerance to the stimulus properties of methylphenidate, phenmetrazine, and phentermine, such that doses approximately two-fold higher than those used acutely were necessary to reproduce the original effect; the potency for the substitution of diethylpropion for the cocaine stimulus was decreased greater than four-fold; and fenfluramine still failed to substitute for the cocaine stimulus. These data suggest that 1) a common mechanism may mediate tolerance to the discriminative stimulus properties of cocaine, methylphenidate, phenmetrazine, and phentermine, and 2) tolerance in the drug discrimination procedure may have potential for establishing a comprehensive evaluation of dependence liability of CNS stimulants. 相似文献
13.
The involvement of dopamine (DA) receptor subtypes in the behavioral effects of CNS stimulants was studied in rats trained to discriminate occaine from saline. In substitution tests, the stimulus effects of 10mg/kg of this substance generalized tod-amphetamine (0.25–1.0 mg/kg) and the selective D2 against LY-171555 (0.05–0.25 mg/kg); but not to the D1 agonist SKF-38393 (5.0–15.0 mg/kg); in combination tests, the D1 antagonist Sch-23390 (0.0625–0.5 mg/kg) significantly blocked, and the D2 antagonist spiperone (0.25–0.5 mg/kg) partially blocked the cocaine cue. These data suggest that the involvement of DA systems in the behavioral effects of cocaine is more complex than either D1
or D2 receptor activation; for example, the stimulus properties of this substance might involve both D1
and D2 receptor activation.Some of these results were presented at the meeting of the Society for Neuroscience, Toronto, 1988 相似文献
14.
The effects of different subcutaneous doses of cocaine (0.63, 1.25, 2.50, 5.00 and 10.0 mg/kg) on self-stimulation in rats were studied. Monopolar nichrome electrodes were implanted in the medial forebrain bundle at the level of the lateral hypothalamus. Six different stimulus parameter combinations (SPC's), inducing different predictable response rates were used. Cocaine showed a dose-related response stimulation, the highest at 10 mg/kg (49.7%); the response depression was very low at all doses and ranged from 3.20–8.23%.In the described experimental conditions apomorphine, amphetamine and cocaine have some properties in common but there are also important differences. With the three compounds response stimulation is related to the total control response rates at the different SPC's and is the highest at the two SPCs inducing the lowest control response rate. The differences in response stimulation associated with low intensity and low frequency SPC's could be related to a different mechanism of action.The response depression, the highest with apomorphine and the lowest with cocaine seems to be related to the stereotype inducing property of the compounds.The obtained effects of the three compounds are discussed in terms of their possible different influence on the dopaminergic system. 相似文献
15.
Dopamine D1 and D2 mediation of the discriminative stimulus properties ofd-amphetamine and cocaine 总被引:1,自引:1,他引:0
Evidence suggests that stimulants such asd-amphetamine and cocaine act presynaptically by increasing the amount of dopamine (DA) available to stimulate postsynaptic DA receptors. Since two subpopulations of DA receptors (D1 and D2) exist, we investigated the role of both of these receptor subtypes in mediating the internal state produced by these stimulants. Two groups of rats (N=8/group) were trained to discriminate intraperitoneal (IP) injections of eitherd-amphetamine (1 mg/kg) or cocaine (10 mg/kg) from saline in a two-lever, water-reinforced, drug discrimination task. After stable performance was established (i.e., more than 85% correct under each training condition), substitution and combination tests were conducted with selective D1 and D2 agonists and antagonists. The D2 agonist quinpirole (0.0313–0.125 mg/kg) mimicked both stimulant cues while the D1 agoinst SKF 38393 (5–20 mg/kg) substituted partially for cocaine but notd-amphetamine. Combination tests with DA antagonists indicated that both the D1 antagonist SCH 23390 (0.0063–0.25 mg/kg) and the D2 antagonist haloperidol (0.125–0.5 mg/kg) attenuated the effects of both stimulants; in addition, the substitution of cocaine (20 mg/kg) ford-amphetamine was blocked by both DA antagonists. The ability of both D1 and D2 antagonists to attenuate the stimulus effects ofd-amphetamine and cocaine raises the possibility that a synergistic (enabling) interaction between D1 and D2 receptors may modulate stimulant cues. 相似文献
16.
Cats were trained to choose between two levers of an operant chamber using interoceptive cues provided by d-amphetamine or saline as the discriminative stimuli. Following training, stimulus generalization was observed to additional doses of d-amphetamine and cocaine, but not to morphine. Clozapine blocked the generalization of the drug discrimination response to d-amphetamine, but had no effect on generalization to cocaine. These data indicate that discriminative stimulus properties of psychomotor stimulants, previously described in rats, are similar in cats. 相似文献
17.
In rats trained to discriminate 10 mg/kg cocaine from 1 mg/kg saline, norcocaine, the N-demethylated metabolite, at doses of 2.5 mg/kg, 5 mg/kg and 10 mg/kg, produced a dose response curve similar to that of cocaine and generalized to cocaine at the two higher doses. As with cocaine, the discriminative stimulus produced by the norcocaine was partially attenuated by the dopaminergic antagonist pimozide and the amine depletor reserpine. Benzoylecgonine, benzoylnorecgonine and ecgonine methyl ester in doses of 10 mg/kg and 20 mg/kg did not generalize to cocaine. 相似文献
18.
RATIONALE: The relative contributions of different dopamine receptor subtypes to the discriminative stimulus effects of cocaine may be influenced by the training dose of cocaine. Substitution tests with dopamine receptor agonists have suggested that the role of dopamine D2-like receptors is diminished relative to that of D1-like receptors at a training dose of 3 mg/kg cocaine compared with a training dose of 10 mg/kg. OBJECTIVES: To test whether dopamine D2-like receptor antagonists were differentially effective at attenuating cocaine's discriminative stimulus effects at different training doses, and to test for the first time an antagonist that is selective for the dopamine D2 receptor within the D2-like receptor subfamily. METHODS: Rats were trained to press one lever after receiving cocaine and another after receiving saline (maintaining >95% drug-appropriate responding). Three dopamine D2-like receptor antagonists (haloperidol, raclopride and L-741,626) were tested in rats trained at 3 mg/kg or 10 mg/kg cocaine. At the lower training dose, the D1-like receptor antagonist SCH 39166 was also tested. RESULTS: The antagonists were not differentially effective between training groups: they all produced parallel, rightward shifts in cocaine's dose-effect function, indicating surmountable antagonism. CONCLUSIONS: The results demonstrate that D2-like receptor antagonists with different affinities for the various D2-like receptors can antagonise the discriminative stimulus effects of cocaine at two training doses. Importantly, antagonism by L-741,626 implies that stimulation of D2 receptors alone (not D3 or D4 receptors) is sufficient to mediate cocaine's discriminative stimulus effects. Finally, the claim that D1-like receptors are preferentially involved at low training doses of cocaine is only consistent with the current findings if indirect stimulation of D2 receptors by low doses of cocaine remains necessary for the expression of the D1-like receptor-mediated effect. 相似文献
19.
R E Chipkin J M Stewart K Channabasavaiah 《Pharmacology, biochemistry, and behavior》1980,12(1):93-98
Male Sprague-Dawley rats were trained to discriminate ethanol (2 g/kg, PO: EtOH) from saline (10 ml/kg, PO: SAL) in a two-bar positively reinforced operant task on a VI 15 sec schedule. After the rats reached criterion performance (greater than 90% correct responses on the appropriate lever), thyrotropin releasing hormone (pyroGlu-His-Pro-NH2: TRH), a metabolite of TRH (His-Pro diketopiperazine: HP), and a structural analog of TRH (HPCA-His-ThiaPro-NH2: OHT) were tested for their ability to antagonize the EtOH cue. These peptides were chosen for their reported ability to reverse ethanol-induced narcosis. However, at doses that did not disrupt performance, TRH, HP, and OHT did not affect the stimulus properties of ethanol at any dose tested, nor did they change the stimulus properties of saline. Naloxone and ACTH(1-10)-NH2 were also tested as ethanol antagonists of the training dose. Pretreatment with either of these compounds failed to alter ethanol-appropriate responding. In addition, (DA1a2-Met5)-enkephalin-ol, (DAla2-Met(O)5)-enkephalin-ol, substance P, delta sleep-inducing peptide, and bombesin were tested for their ability to elicit ethanol appropriate responding. The EtOH cue generalized to none of these peptides. 相似文献
20.
J C Winter 《Pharmacology, biochemistry, and behavior》1984,20(2):201-203
A group of six rats was trained to discriminate the effects of para-methoxyamphetamine (PMA; 3 mg/kg, 15 min pretreatment time) and saline in a two-lever choice task using a fixed ratio 10 schedule of water reinforcement. Stimulus control was assumed to be present when 80% or more of the first ten responses were appropriate for the treatment condition on each of five consecutive days. PMA established stimulus control in each of the subjects. The mean number of sessions prior to the onset of criterion performance was 19 (SE = 2, range = 14-24). A second group of ten rats was similarly trained with lysergic acid diethylamide (LSD; 0.1 mg/kg, 15 min pretreatment time) and saline. In rats trained with PMA, LSD yielded intermediate results, i.e., significantly different from both training conditions. Likewise, the response distribution was intermediate in nature when LSD-trained subjects were tested with PMA. Pizotyline did not antagonize PMA-induced stimulus control in rats trained with PMA and saline but did antagonize the intermediate responding produced by PMA in LSD-trained subjects. It is concluded that PMA-induced stimulus control does not depend upon activation of serotonergic receptors but that PMA does possess some LSD-like effects which are mediated serotonergically. 相似文献