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1.
Arzu Atalay Akan Neriman Şengül Şerife Şimşek Seher Demirer 《Journal of investigative surgery》2013,26(6):369-372
Purpose: Atherosclerosis observations after splenectomy for trauma and hypersplenism suggests a possible role for the spleen in lipid metabolism. The authors examined the effects of splenectomy on serum lipids in rats and also cholesterol-fed rats with experimental atherosclerosis. Methods: This study was designed on rats. The rats were divided into five groups: splenectomy, normal diet (SP-N, n: 8), splenectomy, cholesterol-fed groups (SP-C, n: 8), splenic autotransplantation after splenectomy, normal diet (SA-N, n: 8), splenic autotransplantation after splenectomy, cholesterol-fed groups (SA-C, n: 8) and sham groups (n: 8). Total triglyceride, total cholesterol, HDL (high-density lipoprotein), LDL (low-density lipoprotein), and VLDL (very low-density lipoprotein) levels were determined in 40 rats. The rats were classified into five groups based on the surgical procedures. The spleens were removed and then the rats were fed a normal diet in Group SP-N (n = 8). The spleens were removed and then the rats were fed a diet containing 1% cholesterol in Group SP-C (n = 8). Splenectomy and splenic autotransplantations were performed and then the rats were fed a normal diet in Group SA-N (n = 8). Splenectomy and splenic autotransplantations were performed and then the rats were fed a diet containing 1% cholesterol in Group SA-C (n = 8). The rats were sham-operated in the control group (Group S, n = 8). An active splenic function was shown in rats that underwent splenic autotransplantation in both groups by using Technicium 99 m sulphurcolloide sintiscan on day 30. Blood lipid levels were repeated 6 months later. Results: There was no difference between pre- and postoperative lipid levels in the sham group and SA-N group (p >.05). All lipid levels including HDL were increased significantly in SP-C group (p <.05). Also VLDL and total tryglyceride levels were increased significantly in SP-N and SA-C groups (p <.05). Conclusions: This study showed that the spleen might have an important effect on lipid metabolism and splenic autotransplantation may be protective in conditions with increased lipid levels. 相似文献
2.
M. A. Rocha M. G. F. Sartori M. De Jesus Simões V. Herrmann E. C. Baracat G. Rodrigues de Lima M. J. B. C. Girão 《International urogynecology journal》2007,18(6):645-651
The aim of this study was to evaluate the modifications in the amount of collagen, muscular, and elastic fibers in the mid-urethra
of adult female rats during the pregnancy and after the natural childbirth, cesarean, and after simulated trauma of childbirth.
The authors evaluated the histomorphometric aspects (collagen, muscular, and elastic fibers) in the mid-urethra of 70 animals
distributed in seven groups: group 1 (n = 10)—control, group 2 (n = 10)—pregnant female rats, group 3 (n = 10)—female rats submitted to cesarean, group 4 (n = 10)—female rats with natural childbirth, group 5 (n = 10)—virgin female rats with simulated trauma of childbirth, group 6 (n = 10)—female rats submitted to cesarean followed by simulation of childbirth trauma, and group 7 (n = 10)—female rats with natural childbirth followed by simulation of childbirth trauma. The average concentration of collagen
and elastic fibers and the collagen/muscular fiber correlation in groups 1, 2, and 3 were similar and significantly inferior
to groups 4, 5, 6, and 7. The average of muscular fibers was similar in groups 1, 2, and 3 and significantly superior to groups
4, 5, 6, and 7. Pregnancy and cesarean did not induce alterations in collagen, muscular, and elastic fibers. However, the
vaginal delivery and simulation of childbirth trauma determined the decrease in muscular fibers and the increase in collagen
and elastic fibers and the correlation collagen/muscular fiber. 相似文献
3.
Ilter Ozer Murat Ulas Metin Ercan Yusuf B. Ozogul Neslihan Zengin E. Birol Bostanci 《Journal of investigative surgery》2013,26(4):177-181
The aim of this study is to evaluate the effects of duration of carbon dioxide (CO2) pneumoperitoneum on experimental colonic anastomosis. Forty-eight male Sprague–Dawley rats were used. The rats were divided into three groups. The rats in group 1 (n = 16) underwent laparotomy and colonic anastomosis without pneumoperitoneum. The rats in group 2 (n = 16) and group 3 (n = 16) were subjected to 2 and 4 hours of 12 mm Hg pneumoperitoneum, respectively, before laparotomy and colonic anastomosis. Half of the rats were sacrified on the third postoperative day; and the other half, on the seventh postoperative day. A colonic segment including anastomosis site was resected for histopathologic and biochemical evaluation. On day 3, hydroxyproline levels of the three groups were similar. The edema score of group 2 was significantly higher than that of group 1, and the necrosis score was higher in group 2 than in group 3. The scores of the other histopathologic parameters were similar. On day 7, group 3 showed significantly higher hydroxyproline levels than group 1, and group 1 showed a higher necrosis score than group 3. In conclusion, CO2 pneumoperitoneum of 12 mm Hg for 2 and 4 hours did not result in impaired healing of experimental colonic anastomosis. 相似文献
4.
The objective of this study was to trigger the formation of rat abdominal aortic aneurysm by applying calcium chloride periarterially and then to detect the degree of prevention of aneurysm occurrence by oral introduction of indomethacin in some of the rats. Thirty-one rats were divided into three groups. The infrarenal aorta above the iliac bifurcation of rats was treated with sodium chloride in group 1 (control, n = 7), calcium chloride in group 2 (n = 12), and calcium chloride-indomethacin in group 3 (n = 12) periarterially. The rats of each group were randomly selected at the end of the first, second, and third weeks postoperatively; and vessel diameters of abdominal aortas were measured by digital photography using a micrometer. Aneurysmal development was not observed in any of the rats in the control group. None of the comparisons was statistically significant (p > 0.05). Aneurysmal development was observed in all of the rats in the calcium chloride group. Results from the first, second, and third weeks postoperatively were statistically significant (p < 0.05). A middle aneurysmal development was observed in all rats in the calcium chloride-indomethacin group. Only results from the second and third weeks postoperatively were statistically significant (p < 0.05). Measurements in groups 2 and 3 were statistically significant when compared to group 1 (p < 0.001). However, the mean increase in the indomethacin-treated group (group 3) was only 26.1%. The macroscopic appearance of the control group and an aneurysm induced by calcium-chloride application are shown.Presented at the Sixteenth Annual Meeting of the Mediterranean Association of Cardiology and Cardiac Surgery, Bodrum, Turkey, September 26-29, 2004. 相似文献
5.
The study design was to decrease the damage of spinal cord on the experimentally induced acute spinal cord injury in rats. The objective of this study was to evaluate whether recombinant human erythropoietin (rHu-EPO) and methylprednisolone (MPSS) improve neurological function and histopathological changes if systemically administered after traumatic spinal cord injury. This study included 48 rats that underwent experimental SCI. Forty-eight animals were randomly divided into six groups. Animals constituted a moderate compression of 0.6 N that was produced by application of an aneurysm clip at level T3 for 1 min. rHu-EPO (1,000 and 3,000 U (Unit) per kg of body weight i.p.) and MPSS (30 mg/kg) were administered 5 min after injury, and control group was saline treated. (1) Control group (n=8), (2) MPSS group (n=8), (3) rHu-EPO 1,000 U group (n=8), (4) MPSS + rHu-EPO 1,000 U group (n=8), (5) rHu-EPO 3,000 U group (n=8), and (6) MPSS + rHu-EPO 3,000 U group (n=8). The neurological function and histopathology were evaluated at 24 and 72 h. According to the neurological functional test scores significant improvements between the control group and the other groups that had taken medical treatment were observed (P<0.001). Histopathologically severe ischemic findings were observed in the control group. A significant decrease in ischemic damage was detected in MPSS + rHu-EPO 3,000 U group (P<0.001). The most significant neurological functional and histopathological improvements were observed after systemical administration of MPSS + rHu-EPO 3,000 U and rHu-EPO 3,000 U. Furthermore, the MPSS + rHu-EPO 3,000 U group provides the most improved neurological functional and histopathological recovery. 相似文献
6.
Suleyman Orman Kerem Karaman Banu Isbilen Basok Ucler Kisa Ayse Bahar Ceyran Erdal Birol Bostanci 《Journal of investigative surgery》2018,31(2):153-162
Background: The hypothalamic–pituitary–tyhroid axis is directly affected by drastic changes in energy stores. The aim of the present study was to determine the effects of triiodothyronine (T3) treatment on cut-surface healing of remnant stomach with weight loss alterations after sleeve gastrectomy (SG). Methods: Thirty male Wistar Albino rats were divided into three groups: sham (n = 6), control (n = 12), and experimental (n = 12). Control and experimental group rats underwent sleeve gastrectomy. Experimental group rats received a single dose of T3 (400 mg/100 g) on the first postoperative day whereas control group rats received 0.9% NaCl. All rats were sacrificed on the seventh postoperative day. Results: In the group of rats receiving T3, levels of FT3 were significantly higher and that of FT4 were significantly lower compared with both the control and sham group rats (p <.05). No significant difference was found between control and T3 group rats in terms of weight loss (p >.05). Microscopic examination of the cut surface of remnant stomach in the control group rats revealed significantly more severe tissue necrosis, edema, and disruption of mucosal epithelium than in the T3 group rats (p <.05). On the other hand, bridging of the submucosal and muscular layers, tissue granulation, fibroblast accumulation, neoangiogenesis, and collagen deposition in the T3 group rats were significantly higher than in the control group rats (p <.05). Conclusions: Sleeve gastrectomy did not significantly alter thyroid hormone levels in short term. T3 hormone therapy seems to deliver constructive therapeutic effects for wound healing while causing no adverse effect on weight reduction. 相似文献
7.
We investigated the effect of inguinal canal closure as a new mechanically induced cryptorchid rat model. The effectiveness
of this new model was evaluated by histopathological examination. Thirty-one 21-day-old Wistar rats were divided into four
groups. In groups 1 (n=6), 2 (n=6) and 3 (n=7), unilateral undescended testis was created by performing inguinal canal closure with inguinoscrotal approach. Sham-operated
rats were used as controls in group 4 (n=12). The rats were killed on day 30 after surgery in group 1, day 45 in group 2 and day 60 in group 3. The seminiferous tubular
diameter, number of tubules with mature germ cell and Leydig cell clusters were evaluated. None of the rats were lost during
the study period. Signs of infection were not detected in operation site although antibiotics were not used. Overall only
three (16%) testes descended into scrotum in study groups. The operation time was 3–4 min for each rat. Histopathological
examination revealed detrimental effects of cryptorchidism on testicular growth in study groups. In all groups, except the
sham group, the mean tubular diameter and the number of tubules with mature germ cells in the left testicle were significantly
decreased compared to the right ones. Our findings were in correlation with other experimental studies using different rat
models of cryptorchidism. This new model of cryptorchidism is considered to provide a simple and effective technique for investigating
the impaired development of the testes in cryptorchidism.
Received: 26 September 2000 / Accepted: 27 December 2000 相似文献
8.
We aimed to compare intracavernosal injection (ICI), tail vein injection (IV), and periprostatic injection (PPI) of adipose-derived stem cells (ADSCs) for their ability to improve erectile function in cavernous nerve injury-induced erectile dysfunction (CNIED) rats and to explore the possible mechanism. Eighty-four male SD rats were divided into the sham group (n = 6), BCNI group (bilateral CN crush injury, n = 6), PBS-ICI group (n = 6), PBS-IV group (n = 6), PBS-PPI group (n = 6), ADSC-ICI group (n = 18), ADSC-IV group (n = 18) and ADSC-PPI group (n = 18). ADSCs were labelled with 5-ethynyl-2′-deoxyuridine (EdU), and six rats each in the ADSC-ICI group, ADSC-IV group, and ADSC-PPI group were sacrificed 2, 7, and 28 days after injection. EdU-labelled ADSCs were tracked by immunofluorescence staining. The intracavernosal pressure (ICP)/mean arterial pressure (MAP) ratio, neuronal nitric oxide synthase (nNOS)-positive nerve fibres in the dorsal penile nerve and the smooth muscle/collagen ratio in the cavernosum between groups were also evaluated. ADSCs can significantly improve erectile function through ICI or IV. The two are similar in efficacy and superior to PPI. The mechanism may be that after CN injury, ADSCs are recruited to around the MPG and secrete a variety of neurotrophic factors that promote the repair of the CN, thereby improving erectile function. 相似文献
9.
J. Pirenne M. D'Silva G. Degiovanni N. Jacquet D. L. Dunn 《Transplant international》1993,6(5):258-262
The small bowel (SB), an organ replete with lymphocytes, may provoke graft-versus-host disease (GVHD) after transplantation (Tx). Since tumor necrosis factor (TNF) has been suspected of mediating the tissue lesions of GVHD, we sought to determine whether TNF could be detected in the serum of rats undergoing GVHD after SBTx or lymphocyte transfer. For this purpose, post-operative serum TNF activity was determined in Lewis x Brown after undergoing transplantation of an entire (group 1; n=8) or a segmental (group 2; n=4) Lew SB, or after i. p. injection with lethal doses (500×106) of Lew lymphocytes (group 3; n=3). Control LBNF1 received i.p. small doses (50×106) of Lew lymphocytes (group 4; n=4). Serum TNF activity was assessed using the WEHI bioassay. In rats with acute and lethal GVHD after entire SBTx (group 1) or injection with large doses of lymphocytes (group 3), TNF activity gradually increased and reached high levels by the time the rats were agonal. In segmental SBTx rats (group 2), GVHD was less severe than in entire SBTx rats. Similarly, the increase in TNF activity was less intense and only transient since it had returned to control levels by the time the rats had completely recovered from GVHD. In control rats primed with small doses of lymphocytes (group 4), GVHD did not occur and no increase in TNF activity was detected. We conclude that: (1) GVHD after SBTx or lymphocyte transfer is associated with the appearance of TNF in the serum and (2) the intensity and the reversibility of this phenomenon correlate with clinical severity and lethality of GVHD. These data strongly suggest that TNF is involved in the pathogenesis of GVHD. 相似文献
10.
Effect of etidronate on three-dimensional trabecular structure in ovariectomized or sciatic neurectomized rats 总被引:1,自引:0,他引:1
Nishida A Ito M Uetani M Nakayama T Tanaka T 《Journal of bone and mineral metabolism》2004,22(4):335-340
The purpose of this study was to evaluate the effect of etidronate (EHDP) on three-dimensional (3D) trabecular structure in ovariectomized (OVX) and sciatic neurectomized (NX) rats. Eight-week-old female Lewis rats received ovariectomy (n = 19) or sham operation (OVX-sham; n = 10). OVX rats received either vehicle (OVX-control; n = 9) or EHDP (OVX-EHDP; n = 10). Eight-week-old female Lewis rats received NX (n = 20) or sham operation (NX-sham; n = 10). NX rats received either vehicle (NX-control; n = 10) or EHDP (NX-EHDP; n = 10). EHDP at 5mg/kg or vehicle was subcutaneously injected 5 days a week. The treatment was initiated 2 weeks after surgery and was continued for 2 weeks. At 12 weeks of age, the rats were killed, and we scanned the proximal metaphysis of the tibia; this was done using micro-CT; ( CT20; SCANCO Medical). The recovery of structural parameters was not complete in NX rats compared to OVX rats. The 3D micro-CT images showed that the subcortical spongiosa, which was preserved in OVX rats, had marked loss in NX rats. Furthermore, these trabeculae were not restored after the EHDP treatment. In conclusion, the mechanical driving of the control of trabecular structure is inactive in NX, and EHDP treatment for 2 weeks does not restore this condition. 相似文献
11.
Mehmet Haşim Güner Abdulkerim Olgun Merve Torun Eksal Kargi 《Journal of plastic surgery and hand surgery》2016,50(5):291-297
Background: The main purpose of this study was to assess the effects of ozone gas on the viability of flaps for reconstruction and to determine the optimum application method. The antioxidant, immunomodulatory, and reperfusion effects of ozone gas have been previously assessed, and successful results have been reported. However, only one study has investigated the effect of ozone gas on flap viability. In the present study, it was hypothesised that the antioxidant and reperfusion effects of ozone gas would enhance flap viability.Methods: Forty female Wistar rats were randomly divided into four groups of 10 rats each. A cranial-based, 3?×?11?cm modified McFarlane flap including the panniculus carnosus was raised from the dorsum of a rat and re-sutured to its own bed using 3/0 sharp propylene. Group 1 (n?=?10): no pharmacological agent was used after the operation. Group 2 (n?=?10): vegetable (olive) oil group; vegetable-oil-impregnated gauze was used as a dressing for 7 days. Group 3 (n?=?10): Vegetable (olive) oil with ozone peroxide group; vegetable oil with ozone peroxide-impregnated gauze was used as a dressing for 7 days. Group 4 (n?=?10): Hemo-ozone therapy group; hemo-ozone therapy was applied rectally once every day for 7 days. All rats were sacrificed at the end of week 1 and assessed macroscopically and histopathologically.Results: The proportion of substantive necrosis was less in group 4 than in the other three groups. Survival area ratios were better in groups 2 and 3 than in group 1; however, there was no significant difference between groups 2 and 3. No significant differences in the histopathological scores were observed among the groups.Conclusion: Ozone gas enhanced flap viability. No differences in flap viability were observed between the vegetable oil and vegetable oil with ozone peroxide groups. The greatest benefit ratios were found in the hemo-ozone therapy group. 相似文献
12.
Ascorbic Acid (Vitamin C) and Iloprost Attenuate the Lung Injury Caused by Ischemia/Reperfusion of the Lower Extremities of Rats 总被引:3,自引:0,他引:3
Baltalarli A Ozcan V Bir F Ferda B Aybek H Sacar M Onem G Goksin I Demir S Teke Z Zafer T 《Annals of vascular surgery》2006,20(1):49-55
The objectives of this study were to compare the protective effects of ascorbic acid and iloprost on lung injury caused by
ischemia reperfusion (I/R) of the lower extremities of rats. Wistar albino rats (n = 34) were divided into five groups. In the I/R group (n = 6), the aorta was cross-clamped for 3 hr, followed by 1 hr of reperfusion. In the vitamin C group (n = 8), animals were pretreated with 100 mg/kg ascorbic acid via the left jugular vein before aortic cross-clamping. In the
iloprost group (n = 8), animals were pretreated with 20 ng/(kg · min) iloprost by constant intravenous infusion via the left jugular venous
cannula. In the sham group (n = 6), the abdomen was left open at the same period and a juguler venous line was established. In the control group (n = 6), lungs were removed and blood samples taken immediately after sternotomy. No treatment was given in this group. After
both lungs were removed, biochemical parameters were measured and histopathological evaluation was made. Although the arterial
blood pO2 and HCO3 levels were stastistically significantly high in both the vitamin C and iloprost groups compared to the I/R group, plasma
malondialdehyde (MDA) levels were significantly low. Meanwhile, the MDA levels in the lung tissue were significantly low in
the vitamin C group compared to the I/R group. The MDA level in the lung tissue in the iloprost group was also low compared
to the I/R group, but it was not statistically significant. The lungs of the I/R group displayed intense interstitial leukocytic
infiltration in histopathological examination compared to the other groups. Pretreatment of animals with iloprost and vitamin
C significantly decreased the pulmonary injury characterized by decreased plasma leukocyte sequestration. The results suggest
that both vitamin C and iloprost are useful agents for attenuating the lung injury caused by increased oxidative stress and
neutrophil accumulation after a period of I/R of the lower extremities. 相似文献
13.
《Renal failure》2013,35(4):687-693
AbstractThis study was designed to investigate the protective effects of sitagliptin on renal damage induced by renal ischemia reperfusion (I/R) in rats. For this, rats were randomly divided into four groups (n?=?8): (1) sham group, in which the rats only underwent right nephrectomy; (2) right nephrectomy and left kidney ischemia (1?h) and reperfusion (24?h) group (I/R); (3) 5?mg/kg sitagliptin administrated group, per-oral once a day for two weeks; (4) 5?mg/kg sitagliptin administrated group, per-oral once a day for two weeks before left kidney I/R (n?=?8). Sitagliptin-treated rats that underwent renal I/R demonstrated significant decrease in the serum urea nitrogen and creatinine and also, lipid peroxidation, total oxidant status and malondialdehyde level in the renal tissue when compared to the renal I/R group. Additionally, reduced glutathione, glutathione peroxidase, superoxide dismutase, catalase and total antioxidative capacity were significantly increased after renal I/R in sitagliptin-treated rats. Our histopathological findings were in accordance with these biochemical results. In sum, in the current study all of our results indicated that sitagliptin treatment ameliorated renal damage induced by renal I/R in rats. 相似文献
14.
Ozturk H Ozturk H Gedik S Uzunlar AK Ketani A 《International urology and nephrology》2007,39(1):267-270
In the kidney, prostaglandins represent important physiological modulators of renal hemodynamics and salt and water homeostasis.
In this experimental study of bilaterally adrenalectomized (ADX) rats, we aimed to investigate whether the administration
of selective (celecoxib) inhibitor of COX-2 would alter the morphological and functional changes in rat kidney tissue. Twenty-one
male Sprague–Dawley rats weighing 225–250 g were used. The animals were divided into three groups. Group 1 rats (Sham-control,
n = 7) did not receive any treatment. In group 2 rats (ADX/Untreated, n = 7), bilateral ADX was performed via a single dorsal incision. In group 3 (ADX/COX-2) rats, the same operation was performed
as described for group 2 and then the COX-2 inhibitor celecoxib was administered by gavage for a period of 7 days. On the
7th day of the study, renal function was assessed by measurements of blood urea nitrogen (BUN) and serum creatinine levels.
Biopsies were obtained from the remaining left kidneys before killing the rats. There was no significant difference in the
BUN and creatinine values between the groups. In ADX/Untreated group, capillary congestion in glomerule, inflammation, hemorrhage
and congestion in intertubular area, and cytoplasmic vacuolation in renal tubules was observed. Mild damage was observed in
the ADX/COX-2 group. The number of macrophages was significantly decreased in ADX/COX-2 group when compared to ADX/Untreated
group (P < 0.0001). Our study indicates that celecoxib may be an important factor affecting renal morphological changes after the
bilaterally ADX. 相似文献
15.
《Renal failure》2013,35(2):318-322
AbstractWe investigated the changes in the serum levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6, the pro-inflammatory cytokines, and the possible effect of melatonin on the modulation of these inflammatory molecules after renal ischemia reperfusion (IR). The study was carried out in the laboratory of Department of Pharmacology. Forty-six male Wistar albino rats were divided into five groups as control (n?=?6), positive control (n?=?4), sham (n?=?12), renal IR (n?=?12), and renal IR melatonin (n?=?12). After 1?h renal pedicle occlusion, the blood samples were taken for the measurement of cytokine levels at second hour of the reperfusion. The rats were sacrificed after 24?h of reperfusion for histopathological evaluation. Melatonin or vehicle was administrated to IR rats. Lipopolysaccharide (LPS) was administered to the positive control group and the blood was taken at fourth hour. Serum TNF-α levels increased significantly in renal IR and LPS groups. Serum IL-6 levels were not different from control except the LPS group. There was no significant correlation between the serum TNF-α levels and the histopathological score after renal IR. Melatonin treatment reversed the increase of serum TNF-α levels and histopathological injury in renal tissue after renal IR. Melatonin may have a protective effect by reducing the serum level of TNF-α in renal IR. 相似文献
16.
Sungurtekin H Ozden A Kaleli I Sungurtekin U Gonullu M 《Journal of Hepato-Biliary-Pancreatic Surgery》1999,6(4):405-409
The purpose of this study was to examine the effect of endogenous somatostatin hormone on bacterial translocation in obstructive
jaundiced rats. Five groups of rats were studied: group I (n = 10), non-operated group (control); group II (n = 10), sham-operated group which underwent laparotomy and dissection of portal elements, while the common bile duct was not
ligated and somatostatin was not injected; group III (n = 10), same as group II, plus injection of somatostatin; group IV (n = 10), common bile duct was ligated with laparotomy but somatostatin was not injected; group V (n = 10), same as group IV, plus somatostatin injection. The blood was analyzed for somatostatin, alkaline phosphatase, and
bilirubin levels on the third and tenth days in all animals. At study termination (tenth day), peritoneal swab and blood cultures
were taken, and liver, spleen, lung, and mesenteric lymph nodes were harvested for microbiological studies. Bacterial translocation
levels were higher in groups III, IV, and V when compared with levels in groups I and II. Similar translocation levels were
obtained when blood somatostatin levels were comparable. However, the highest translocation rate was found in groups IV and
V in which the blood somatostatin level was also higher when compared with that in other groups. This finding shows that blood
somatostatin level is increased in obstructive jaundice. This may explain the bacterial translocation and related sepsis found
in obstructive jaundice.
Received for publication on Feb. 2, 1999; accepted on May 3, 1999 相似文献
17.
S. Okamoto Carlos O. Corso Rosmarie Leiderer Wolfgang Rascher Yuzo Yamamoto Yoshio Yamaoka Konrad Messmer 《Transplant international》2000,13(6):428-435
Hypotension in brain-dead organ donors is considered a determinant factor of graft viability. The aim of this study was to
elucidate the role of hypotension in brain-death associated impairment of hepatic microcirculation and function. Male Sprague-Dawley
rats with an intracranial balloon were used. Group I (n = 7) served as sham controls. In group II (n = 7) brain death was induced through inflation of an intracranial balloon. In group III (n = 7) hypotension without brain death was induced by means of pentobarbital. In group II, a steep rise of arterial pressure
was followed by a fall to a lower level (P < 0.01, vs. group I). Also in group III arterial pressure was lower (P < 0.01, vs. group I). In group II, bile production was diminished (P < 0.05). Impaired sinusoidal perfusion (P < 0.01) and enhanced leukocyte endothelium interaction (P < 0.05) were documented in hepatic microvasculature. Electron microscopic analysis revealed vacuolization of hepatocytes;
these changes were not observed in group III. Brain death induces specific changes of liver microcirculation, function and
histomorphology. Independent of associated hypotension, brain death per se impairs donor liver graft quality.
Received: 28 September 1999 Revised: 5 May 2000 Accepted: 13 September 2000 相似文献
18.
Background: Previous studies showed that nonsteroidal anti-inflammatory (NSAi) drugs suppressed prostaglandin synthesis and were able to prevent adhesion formation following surgical trauma to the peritoneum. The selective suppression innammatory cascade may prevent adhesion formation. Therefore, we planned this study to experimentally evaluate the effects of Rofecoxib, the selective cyclo-oxygcnase-2 inhibitor, in postoperative intraperitoneal adhesions in an animal model.Methods: Male Sprague-Dawley rats were divided into three groups of 10. All rats underwent midline laparotomy under ketamine anaesthesia (25 mg/kg im). In group 1 (n = 10), the sham operation group (SG) ; abdominal walls were closed without any process after 2 minutes. In Group 2 (n = 10), the control group (CG) ; standard serosal damage was constituted and the abdominal wall was closed. In group 3 (n = 10), the COX-2 group (COXG), after serosal damage, the abdominal wall was closed. A 12 mg/kg/day dose of was given orally to the rats during one week. On the 7th postoperative day, all rats were sacrificed and intra-abdominal adhesions were evaluated both macroscopically and microscopically.Results: Macroscopically, no serious adhesion formations were seen in the SG. Multiple adhesion format ions of the CG were significantly more than those of the SG (p < 0.0001). It was determined that adhesions of the COXG diminished (p < 0.0001) when macromorphological adhesion scale results of the COXG were compared with those of the CG. The adhesion scores of the CG were compared microscopically with those of the COXG and granulation tissue formation and fibrosis in the COXG were found to be significantly less than those of the CG (respecti vely p = 0.002, p < 0.0001).Conclusions: We were of the opinion that Rofecoxib, the selective cyclo-oxygenase inhibitor, was effective in the prevention of postoperative peritoneal adhesions. 相似文献
19.
Yoshihisa Yatabe Shumpei Miyakawa Teruo Miyazaki Yasushi Matsuzaki Naoyuki Ochiai 《Journal of orthopaedic science》2003,8(3):415-419
To investigate the effects of taurine administration on exercise, we studied taurine concentrations in rat skeletal muscles
after endurance running and the duration of running time to exhaustion, with and without taurine administration. For study
1 we divided 40 male SD rats into two groups: endurance exercise group (n = 20) and sedentary control group (n = 20). Each was further divided into two groups; one received distilled water (n = 10) and the other taurine solution in water 0.5 g/kg/day orally (n = 10) for 2 weeks. The exercise group performed treadmill running (60 min) once only after their nursing period. For study
2, we divided 10 male SD rats into two groups; one (n = 5) received taurine 0.5 g/kg/day, and the other (n = 5) received no taurine for 2 weeks; the two groups then performed treadmill running to exhaustion. In study 1, taurine
administration increased taurine concentrations in leg skeletal muscles, whereas the concentrations were significantly lower
in the exercised groups without taurine administration. Taurine administration reduced the decrease in taurine concentration
in skeletal muscles on exercise. In study 2, the duration of running time to exhaustion was significantly increased by taurine
administration. We concluded that peroral administration of taurine maintains the taurine concentration in skeletal muscle
on exercise and up-regulates physical endurance.
Received: October 11, 2002 / Accepted: December 17, 2002
RID="*"
ID="*" Offprint requests to: Y. Yatabe
Acknowledgments. We express our appreciation to H. Ohmori, Ph.D. for animal exercise and to the Chemical Analysis Center, University of Tsukuba
for the HPLC analysis. 相似文献
20.
Histopathological and biochemical comparisons of the protective effects of amifostine and l‐carnitine against radiation‐induced acute testicular toxicity in rats
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T. Aktoz M. Caloglu V. Yurut‐Caloglu O. Yalcin N. Aydogdu D. Nurlu E. Arda O. Inci 《Andrologia》2017,49(9)
The aim of this study was to compare the radioprotective efficacies of amifostine (AMI) and l ‐carnitine (LC) against radiation‐induced acute testicular damage. Thirty Wistar albino rats were randomly assigned to four groups: control (n = 6), AMI plus radiotherapy (RT) (n = 8), LC plus RT (n = 8) and RT group (n = 8). The rats were irradiated with a single dose of 20 Gy to the scrotal field. LC (300 mg/kg) and AMI (200 mg/kg) were given intraperitoneally 30 min before irradiation. The mean seminiferous tubule diameters (MSTDs) were calculated. Testicular damage was evaluated histopathologically using Johnsen's mean testicular biopsy score criteria. Malondialdehyde (MDA) and glutathione levels were measured in tissue samples. AMI plus RT and LC plus RT groups had significantly higher MSTDs than those in the RT group (p = .003 and p = .032 respectively). MDA values of both AMI plus RT and LC plus RT groups were significantly lower than those in RT group (p < .004 and p < .012 respectively). As a result, AMI and LC have a similar radioprotective effect against radiation‐induced acute testicular damage, histopathologically and biochemically. 相似文献