降糖孜亚比提片对糖尿病小鼠肝脏的影响

目的研究降糖孜亚比提片对糖尿病肝脏的降糖作用。方法采用四氧嘧啶糖尿病小鼠模型对降糖孜亚比提片进行研究。结果对四氧嘧啶引起的高血糖小鼠的血糖有明显的抑制作用;增加肝糖原的含量,减少肝糖原的输出;并且保护肝脏。结论降糖孜亚比提片具有改善糖尿病肝脏的作用。     

二氢杨梅素对糖尿病小鼠降糖作用研究

目的:研究藤茶中二氢杨梅素的降糖作用。方法:一次性尾静脉注射四氧嘧啶(75mg/kg)建立糖尿病小鼠模型,以二甲双胍为阳性对照,研究二氢杨梅素对糖尿病小鼠的体重、摄食量、饮水量、空腹血糖、糖耐量、糖原、抗氧化能力、血清胰岛素和胰腺组织的影响。结果:二氢杨梅素100、150 mg/kg对四氧嘧啶致病小鼠能极显著降低血糖;提高糖尿病小鼠的糖耐量,减少四氧嘧啶对胰岛的损害,促进糖尿病小鼠肝糖原的合成和胰岛素的分泌;提高糖尿病小鼠的抗氧化能力。结论:二氢杨梅素对四氧嘧啶糖尿病小鼠有降糖作用,其降糖机制可能是通过提高抗氧化作用减轻四氧嘧啶对肝脏和胰岛B细胞的损伤,促进了肝糖原和胰岛素的合成。     

麦冬总皂苷对实验性高血糖小鼠的降糖作用

目的：研究麦冬总皂苷对实验性糖尿病小鼠血糖的影响。方法：麦冬总皂苷（100,200,400 mg/kg）灌胃,测定正常小鼠及由四氧嘧啶、肾上腺素、葡萄糖所致高血糖小鼠血糖水平。结果：麦冬总皂苷对四氧嘧啶、肾上腺素及葡萄糖引起的小鼠高血糖均有抑制作用。结论：麦冬总皂苷对实验性高血糖小鼠有明显的降糖作用。     

丹蛭降糖胶囊对糖尿病小鼠血糖、耐力及免疫功能的影响

目的：观察丹蛭降糖胶囊对四氧嘧啶致糖尿病小鼠血糖、耐力及免疫功能的影响。方法：四氧嘧啶诱导小鼠糖尿病模型,除正常组外,设模型组、西药对照组、中药对照组及丹蛭降糖胶囊低、中、高剂量组,给药15天后,测定小鼠体重、空腹血糖,进行小鼠耐力（游泳时间）试验,进行免疫器官脾脏和胸腺称重并计算脏体系数。结果：丹蛭降糖胶囊能显著降低四氧嘧啶致糖尿病小鼠空腹血糖水平,并明显增加体重,增强耐力,增加脾脏和胸腺脏体系数。结论：丹蛭降糖胶囊对糖尿病小鼠具有降糖、增强耐力和免疫力作用。     

苦瓜总皂苷降血糖作用的研究

目的 观察苦瓜总皂苷对正常小鼠、肾上腺素性高血糖小鼠及四氧嘧啶性糖尿病家兔血糖及胰岛素水平的影响.方法 小鼠ip肾上腺素及家兔耳缘iv四氧嘧啶制备糖尿病模型,苦瓜总皂苷ig给药,葡萄糖氧化酶法测定血糖.结果 正常小鼠ig苦瓜总皂苷后,其血糖与给药前及对照组相比无明显差异;用苦瓜总皂苷连续ig给药7 d,大、中剂量组对肾上腺素性高血糖小鼠有明显降血糖作用;对四氧嘧啶性糖尿病家兔有显著降血糖作用.结论 苦瓜总皂苷对肾上腺素性高血糖小鼠和四氧嘧啶性糖尿病家兔具有较好的降血糖作用,但对正常小鼠血糖及四氧嘧啶性糖尿病家兔的胰岛素无明显影响.     

知母皂苷对小鼠血糖的影响

目的:研究知母皂苷对正常小鼠和糖尿病小鼠糖耐量及空腹血糖的影响,探讨其降糖作用机制.方法:测定知母皂苷对α-葡萄糖苷酶活性的抑制;正常小鼠口服给药,测定空腹血糖和糖耐量;四氧嘧啶致糖尿病小鼠模型口服给药,测定空腹血糖及糖耐量.结果:知母皂苷对正常小鼠无降血糖作用,但可使糖耐量曲线趋于平缓;知母皂苷能够显著提高糖尿病小鼠糖耐量,降低实验性糖尿病小鼠的空腹血糖.结论:知母皂苷具有一定的降糖作用.     

辣木粉调脂降糖作用研究北大核心CSCD

目的:探讨辣木粉调脂降糖作用。方法:利用对小、大鼠高脂血症模型评价辣木粉的降脂作用;利用对正常小鼠血糖的影响、四氧嘧啶糖尿病小鼠模型的降糖作用及对Ⅰ型糖尿病小鼠模型的影响评价辣木粉的降糖作用;利用对高脂、Ⅱ型糖尿病大鼠模型试验的影响评价辣木粉的调脂降糖作用。结果:辣木粉各剂量组能显著降低大、小鼠高脂血症模型中TC、LDL-C的含量;辣木粉各剂量组能明显升高大鼠高脂血症模型HDL-C的含量;辣木粉三个剂量组对正常小鼠血糖无明显影响;辣木粉各剂量组能显著降低四氧嘧啶糖尿病小鼠模型血糖含量的作用;辣木粉8. 0g/kg组对Ⅰ型糖尿病小鼠模型血糖有明显的降低作用;辣木粉6. 0g/kg、3. 0g/kg组能显著降低高脂Ⅱ型糖尿病大鼠模型血糖、TG、TC及LDL-C含量;辣木粉1. 5g/kg组能显著降低TC含量。结论:辣木粉具有较好的调脂降糖作用,其作用机制有待进一步研究。     

藏药降糖舒胶囊对四氧嘧啶糖尿病小鼠的防治作用研究

目的 研究藏药降糖舒胶囊对四氧嘧啶所致糖尿病小鼠的血糖的调节作用.方法 从小鼠尾静脉注射100m g/kg的四氧嘧啶造成糖尿病小鼠模型,以藏药降糖舒胶囊1,2,3 g/kg连续灌胃糖尿病小鼠7d后,测定其空腹血糖并进行糖耐量实验.结果 藏药降糖舒胶囊2,3g/kg具有降低糖尿病模型小鼠空腹血糖及餐后血糖的作用,且降血糖效果随其剂量增加而增强.结论 藏药降糖舒胶囊对四氧嘧啶糖尿病小鼠的高血糖有防治作用.     

辣木粉调脂降糖作用研究

目的:探讨辣木粉调脂降糖作用。方法:利用对小、大鼠高脂血症模型评价辣木粉的降脂作用;利用对正常小鼠血糖的影响、四氧嘧啶糖尿病小鼠模型的降糖作用及对Ⅰ型糖尿病小鼠模型的影响评价辣木粉的降糖作用;利用对高脂、Ⅱ型糖尿病大鼠模型试验的影响评价辣木粉的调脂降糖作用。结果:辣木粉各剂量组能显著降低大、小鼠高脂血症模型中TC、LDL-C的含量;辣木粉各剂量组能明显升高大鼠高脂血症模型HDL-C的含量;辣木粉三个剂量组对正常小鼠血糖无明显影响;辣木粉各剂量组能显著降低四氧嘧啶糖尿病小鼠模型血糖含量的作用;辣木粉8. 0g/kg组对Ⅰ型糖尿病小鼠模型血糖有明显的降低作用;辣木粉6. 0g/kg、3. 0g/kg组能显著降低高脂Ⅱ型糖尿病大鼠模型血糖、TG、TC及LDL-C含量;辣木粉1. 5g/kg组能显著降低TC含量。结论:辣木粉具有较好的调脂降糖作用,其作用机制有待进一步研究。     

铁皮石斛膏降糖作用的研究

目的:观察铁皮石斛膏的降糖作用.方法:采用四氧嘧啶糖尿病小鼠模型观察铁皮石斛膏的降糖作用, 并观察其对糖耐量的影响.结果:铁皮石斛膏3g(生药)/kg剂量灌胃给药可降低四氧嘧啶所致糖尿病模型小鼠的血糖值,并明显改善该模型小鼠的糖耐量,减少其血糖曲线下面积;6g(生药)/kg剂量对正常小鼠糖耐量也有一定改善作用.结论:铁皮石斛膏具有一定的降低血糖和改善糖耐量的作用.     

鹰嘴豆降血糖有效部位的药效学筛选

目的：对鹰嘴豆4个提取部位对正常和高血糖模型小鼠血糖值的影响进行研究，并筛选出其中的有效部位，为进一步研究奠定基础。方法：分别对正常小鼠、肾上腺素高血糖小鼠和四氧嘧啶糖尿病小鼠灌胃4个提取部位，一周后测定血糖值和肝糖原含量。结果：鹰嘴豆提取部位Ⅲ可显著性降低正常小鼠和肾上腺素高血糖小鼠的血糖值，对四氧嘧啶糖尿病小鼠血糖无作用。结论：鹰嘴豆提取部位Ⅲ在降血糖筛选实验中作用最显著，故将其做为有效部位进行进一步研究。     

Antihyperglycaemic effect and acute toxicity of Securigera Securidaca L. seed extracts in mice

The antihyperglycaemic activity of a Securigera securidaca aqueous infusion and an ethanol maceration extract of seeds was studied in normoglycaemic, glucose-induced hyperglycaemic and alloxan-induced diabetic mice. The acute toxicity of the ethanol extract was more than that of the aqueous one. The phytochemical analysis showed that the seed extracts were rich in flavonoids. The intraperitoneal and oral administration of the aqueous and ethanol extracts significantly reduced blood glucose in alloxan-induced diabetic mice. In normoglycaemic and glucose-induced hyperglycaemic mice, the blood glucose levels were not significantly different from the control. Glibenclamide was not able to lower blood glucose in alloxan-induced diabetic mice, while it significantly lowered the blood sugar in normoglycaemic mice. The results indicate that S. securidaca seed extracts significantly reduce blood glucose in alloxan-induced diabetic mice by a mechanism different from that of sulfonylurea agents.     

桑叶多糖降血糖作用及其机制研究

目的探讨桑叶多糖降血糖的作用机制。方法以四氧嘧啶诱导小鼠糖尿病模型,桑叶多糖以0.25、0.50、1.00 g/kg ig给药,连续6周,每周称体质量;在实验2、4、6周末各测空腹血糖;实验末期进行糖耐量试验,测定糖化血清蛋白、血清胰岛素水平;测定肝组织肝糖元、肝匀浆蛋白水平及己糖激酶(HK)、丙酮酸激酶(PK)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)水平。结果桑叶多糖能明显缓解糖尿病小鼠症状;具有明显控制糖尿病小鼠空腹血糖值升高,降低糖化血清蛋白和血糖曲线下面积的作用;具有促进血清胰岛素、肝HK的分泌和肝糖元的合成,提高肝SOD活性,降低肝MDA水平,促进体质量恢复的作用;桑叶多糖中剂量还具有促进肝PK分泌的作用,而对肝GSH-Px活性影响不明显;糖尿病小鼠肝脏、肾脏和脾脏指数均增高,桑叶多糖具有降低脏器指数的作用。结论桑叶多糖通过提高四氧嘧啶糖尿病小鼠抗氧化能力,使胰岛素分泌增加,同时提高肝HK、PK活性等综合作用,促使血糖进入肝细胞,使肝糖元合成增加,葡萄糖氧化分解加快,从而达到调节糖代谢、降低血糖、改善糖尿病症状的作用。     

Antihyperglycemic and antilipidperoxidative effects of dry matter of culture broth of Inonotus obliquus in submerged culture on normal and alloxan-diabetes mice

AIM OF THE STUDY: The antihyperglycemic and antilipidperoxidative effects of the dry matter of culture broth (DMCB) of Inonotus obliquus were investigated. MATERIALS AND METHODS: The normal, glucose-induced hyperglycemic and alloxan-induced diabetic mice were used to evaluate the antihyperglycemic and antilipidperoxidative effects of the DMCB of Inonotus obliquus. RESULTS: Treatment with the DMCB (500 and 1000 mg/kg body weight) exhibited a mild hypoglycemic effect in normal mice, and failed to reduce the peak glucose levels after glucose administration. However, euglycemia was achieved in the DMCB of Inonotus obliquus (1000 mg/kg) and glibenclamide-treated mice after 120 min of glucose loading. In alloxan-induced diabetic mice, the DMCB (500 and 1000 mg/kg body weight for 21 days) showed a significant decrease in blood glucose level, the percentages reduction on the 7th day were 11.90 and 15.79%, respectively. However, feeding of this drug for 3 weeks produced reduction was 30.07 and 31.30%. Furthermore, the DMCB treatment significantly decreased serum contents of free fatty acid (FFA), total cholesterol (TC), triglyceride (TG) and low density lipoprotein-cholesterol (LDL-C), whereas effectively increased high density lipoprotein-cholesterol (HDL-C), insulin level and hepatic glycogen contents in liver on diabetic mice. Besides, the DMCB treatment significantly increased catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities except for decreasing maleic dialdehyde (MDA) level in diabetic mice. Histological morphology examination showed that the DMCB restored the damage of pancreas tissues in mice with diabetes mellitus. CONCLUSIONS: The results showed that the DMCB of Inonotus obliquus possesses significant antihyperglycemic, antilipidperoxidative and antioxidant effects in alloxan-induced diabetic mice.     

枸杞果柄对四氧嘧啶糖尿病小鼠血糖的影响

目的 探讨枸杞果柄水煎剂对四氧嘧啶糖尿病小鼠血糖的影响.方法 制备四氧嘧啶糖尿病小鼠模型,将其分为降糖灵治疗组、低剂量枸杞果柄治疗组、高剂量枸杞果柄治疗组、模型对照组,另设正常对照组(非糖尿病小鼠).前3组小鼠分别以25 mg·kg-1·d-1降糖灵水溶液、5.0 g·kg-1·d-1枸杞果柄水煎剂、7.5 g·kg-1·d-1枸杞果柄水煎剂灌胃,后2组小鼠均灌服等量的生理盐水.14 d后测定小鼠空腹血糖.结果 给药14 d后降糖灵治疗组、低剂量枸杞果柄治疗组小鼠血糖较治疗前有极显著下降(P<0.01),高剂量枸杞果柄治疗组小鼠血糖较治疗前有显著下降(P<0.05);降糖灵治疗组、不同剂量枸杞果柄治疗组血糖降低平均值与模型对照组、正常对照组血糖变化平均值之间均存在极显著差异(P<0.01).结论 一定剂量的枸杞果柄水煎剂对四氧嘧啶糖尿病小鼠血糖有降低作用.     

川麦冬多糖降血糖实验研究

目的:考察川麦冬多糖的降血糖作用。方法:用四氧嘧啶诱导的糖尿病小鼠模型,以空腹血糖值为指标研究川麦冬多糖提取物的降血糖作用。结果:川麦冬多糖(400mg/kg)能显著降低四氧嘧啶引起的糖尿病小鼠的空腹血糖和提高血清胰岛素水平。结论:川麦冬多糖具有降血糖作用。     

Antidiabetic and hypolipidemic effects of Momordica cymbalaria Hook. fruit powder in alloxan-diabetic rats

This study was undertaken to investigate the effect of Momordica cymbalaria fruit powder on blood glucose and other biochemical parameters in alloxan-induced diabetic rats. The treatment was given for 15 days. After the treatment, a significant reduction was observed in fasting blood glucose levels in the treated diabetic rats, but no hypoglycaemic activity in the treated normal rats. M. cymbalaria treatment showed considerable lowering of serum cholesterol and triglycerides in the treated diabetic group. There was a significant improvement in hepatic glycogen level in treated diabetic rats close to normal level after the treatment with M. cymbalaria. These results suggest that the M. cymbalaria fruit powder possesses antidiabetic and hypolipidemic effects in alloxan-induced diabetic rats.     

Hypoglycemic effects of Potentilla fulgens L in normal and alloxan-induced diabetic mice

Tap roots of Potentilla fulgens L. traditionally chewed along with betel nut (Areca catechu) and betel leaves (Piper betel), are commonly used by local practitioners for various types of ailments. The crude methanolic extract of the roots was tested for its effects in normoglycemic and alloxan-induced diabetic mice. Hypoglycemic activity was observed to be dose- and time- dependent. The extracts reduced blood glucose level 2 h following administration in both normal and alloxan-induced diabetic mice. In alloxan-induced diabetic mice blood glucose was markedly reduced by 63%, while in normal mice a 31% reduction was observed 24 h after the effective dose of extract was administered. Further, in the diabetic mice a prolonged anti-hyperglycemic action was observed where glucose levels was, found to be significantly low (79%) when compared with control even on the third day. Glucose tolerance was also improved in both normal and diabetic mice. The results were compared against those of insulin, glibenclamide, metformin, and the probable mechanism of action is discussed.     

番石榴多糖对糖尿病小鼠的血糖及抗氧化能力的影响

目的:观察番石榴多糖对四氧嘧啶诱导的糖尿病小鼠血糖及抗氧化水平的影响。方法:通过对小鼠腹腔注射四氧嘧啶建立糖尿病小鼠模型,将小鼠分成5组:正常对照组、糖尿病对照组、番石榴多糖Ⅰ组、番石榴多糖Ⅱ组、格列苯脲组。在给小鼠灌胃多糖3 d和10 d后,采用血糖仪检测小鼠血糖值,第10日麻醉处死小鼠,分别检测小鼠血清和肝脏中的SOD和MDA水平。结果:与糖尿病对照组比较,灌喂番石榴多糖的两组小鼠的血糖值均较低,同时两组小鼠的血清及肝脏中的T-SOD含量较高,MDA含量较低。结论:番石榴多糖能够显著降低糖尿病小鼠血糖,提高其抗氧化能力,是一种潜在的糖尿病治疗药物。     

苦瓜不同部位对实验性糖尿病小鼠血糖的影响

目的 研究苦瓜不同部位对实验性糖尿病模型小鼠体质量、进食量、进水量和血清中血糖值( GLU)、总胆固醇(TC)、甘油三酯(TG)、超氧化物歧化酶(SOD)、丙二醛(MDA)和游离脂肪酸(NEFA)的影响.方法 雄性昆明种小鼠喂以高脂饲料(10％蛋黄,10％猪油,1％胆固醇,0.2％胆盐,78.8％基料)1个月,诱发胰岛素抵抗,继以腹腔注射低剂量四氧嘧啶(90mg·kg-1),使之产生高血糖症,建立实验性糖尿病小鼠模型.苦瓜水提物冻干粉经100％甲醇回流提取得部位Ⅰ,残渣为部位Ⅱ.分别以10 g·kg-1(相当于生药材重量)的剂量灌胃给药,4周后测定小鼠空腹血糖、糖耐量、血液各项生化指标.结果 苦瓜部位Ⅰ和部位Ⅱ提取物均能缓解由糖尿病所引起的多食多饮病症,降低实验性糖尿病小鼠的GLU、NEFA、TC和MDA值(P＜0.05),升高实验性糖尿病小鼠SOD值(P＜0.05).结论 苦瓜部位Ⅰ和部位Ⅱ提取物能够降低实验性糖尿病小鼠的血糖值,并可调节其血脂代谢,且部位Ⅰ效果更好.