抗胰岛素的单克隆抗体

用猪胰岛素免疫的 BALB/C 小鼠脾细胞,与小鼠骨髓瘤细胞 NS-1融合,获得两株分泌抗猪胰岛素单克隆抗体的杂交瘤细胞,命名为4B9和3B2。其中,以4B9细胞的抗体分泌能力最强。其培液抗体的 ELISA 效价为10~(-2)～10~(-3);注入同系小鼠腹腔诱生的腹水抗体效价达10~(-6)～10~(-7)。其分泌抗体属 IgG_1亚类;对猪胰岛素的亲和常数(Ka)为5.86×10~9L/mole;与用 DNA 重组技术制备的人胰岛素,呈交叉反应。     

八种甾体化合物的雌激素活性及对兔子宫胞浆雌二醇受体亲和力比较

本文比较了炔诺酮肟(NETO)、炔诺酮(NET)、醋炔诺酮肟(NETO-Ac)、醋炔诺酮(NET-Ac)、18-甲基炔诺酮肟(NGTO)、18-甲基炔诺酮(NGT)、醋18-甲基炔诺酮肟(NGTO-Ac)和醋18-甲基炔诺酮(NGT-Ac)8种化合物的雌激素活性及对兔子宫胞浆雌二醇(E_2)受体的亲和力。实验结果证实:NETO的雌激素活性及对E_2受体的亲和力比母体NET明显增强。NETO-Ac及NGTO-Ac也比相应的母体化合物NET-Ac及NGT-Ac有所增强,表明C_3位酮基肟化后,雌激素活性及对受体的亲和力都有增强的趋势。而炔诺酮类C_(18)甲基化后,雌激素活性及对雌二醇受体的亲和力均比相应的母体化合物有所下降。     

血栓素B_2酶免疫分析

本文介绍了血小板释放TXB_2的酶免疫分析法。用混合酸酐法交联β-半乳糖苷酶与TXB_2。通过双抗体沉淀法分离免疫复合物与游离TXB_2。以4-甲基伞形酮-β-D-半乳糖苷作底物测定酶促反应产物的荧光强度。该方法TXB_2最低检测值<0.008pmol(<3.125pg),批内变异系数为5.9％,批间变异系数为5.4％。酶免疫分析法与放射免疫分析法测定血小板样品进行比较,直线相关系数r=0.955,回归方程Y=0.811x+1.397。     

复方左旋18-甲基炔诺酮微球的研究

左旋18-甲基炔诺酮(LNG)与雌二醇(E_2)按5:2重量比配伍,以天然可生物降解的明胶为材料,用相分离法制备了复方左旋18-甲基炔诺酮微球,平均球径为10～40μm,包封率达65％～75％。制成注射液后,经加速与室温观察具有一定的物理化学稳定性,其热解活化能为134.4KJ/mol,体外溶出试验与不包球的注射液相比,T_(1/2)有极显著性差异(P<0.01),说明制成微球后具有明显的缓释作用。经复方与单方的微球注射液组织学对比观察,复方可减轻小鼠卵巢充血的副作用,提示临床应用复方时,LNG引起不规则出血等副作用有可能得以改善。     

放射免疫测定法及其临床应用 血浆18-羟-11-去氧皮质酮(18-OH-DOC)放免测定(不经层析)及其临床应用

在 Erlager 法的基础上,适当改进反应条件,在 pH 8～9条件下,分别采用80°～85℃反应1小时和室温(20°～28℃)反应42小时两种方法制备18-OH-DOC-C_3-羧甲基肟。再用混合酸酐法制备18-OH-DOC-3-BSA,半抗原与载体蛋白的克分子比例分别达27:1和24:1。采用皮内多点注射法免疫动物,在免疫期间,对各兔的抗血清质量进行了监测和比较,选择最佳抗体建立了不经层析的血浆     

兔抗VIP抗体制备及特性研究

用碳二亚胺制备血管活性肠肽(VIP)一牛血清白蛋白连接物免疫新西兰白兔,获得优质抗体R3-6,最终滴度为1:450,000,亲和常数为5.05 x 10~10M-1,结合位点数为88.07pM.该抗体与促胰液素、胰高糖素等7种胃肠激素在浓度为10~-8M时无明显交叉反应,与VIP10～28的反应表明本抗体为抗整个VIP分子的抗体。应用该抗体进行人血浆VIP的放射免疫分析,其灵敏度、精确度及回收率均较满意。     

荧光分光光度法测定复方甲基炔诺酮片中炔雌醇的含量

本文应用荧光分光光度法测定复方甲基炔诺酮片中炔雌醇的含量。方法系根据Kober反应的基本原理,采用混合试剂[硫酸-水(3:1) 0.5％(W/V)对苯二酚]激发荧光,荧光强度比较稳定,再现性较好,甲基炔诺酮和辅料基本不干扰,平均回收率的标准偏差低于4％。本法比过去的方法简便迅速且适用于单片分析。     

口服短效避孕药治疗药物流产后出血时间延长的临床观察

目的:观察短效复方18-甲基炔诺酮对药物流产后出血时间延长的治疗效果。方法:对71例药物流产后出血时间超过2周,B超显示宫腔无孕囊、宫腔形态基本正常的妇女,随机分为两组,观察组口服短效复方18-甲基炔诺酮;对照组口服维生素C、止血敏,两组均口服抗生素及宫缩剂。结果:两组3 d内止血者分别为96.05%、15.15%,下次月经复潮时间为(28.26±2.34)d、(34.37±3.15)d,两组止血时间比较均有显著性差异(P<0.01),两组月经量及行经天数比较无显著性差异(P>0.05)。结论:用短效复方18-甲基炔诺酮治疗药物流产后出血时间延长具有效果好、用药方便、价廉的优点,值得临床推广。     

甾体酮肟的合成

本文以炔孕酮、△~6-炔孕酮、dl-18-甲基炔诺酮为原料,经中间体2-羟亚甲基物(Ⅱ)与亚硝酸反应得相应的2-酮肟-3-酮物(Ⅲ_(a-c)),再与盐酸羟胺缩合便得2,3-双酮肟(Ⅳ_(a-c)),并确证了它们的结构。其中Ⅳ_c对大鼠子宫胞浆黄体酮受体具有一定结合能力。     

肌酸激酶同工酶CK-MM和CK-BB单克隆抗体的研制与鉴定

肌酸激酶同工酶CK-MM和CK-BB单克隆抗体杂交瘤细胞系的建立与特异性分析表明,经PAP-McAb-PAP检测系统和ELISA筛选的特异性抗体,经4次克隆后,共获得3株稳定分泌抗CK-MM,2株抗CK-BB特异性抗体,其中CK-MM-McAb与CK-MM,CK-BB呈特异性反应,效价可达10~(-9),而与CK-BB,Alb,Mb无交叉反应;CK-BB-McAb与CK-BB,CK-MB呈特异性反应,H_8的效价为10~(-10),而且与CK-MM,Alb,Mb无交叉反应。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.