胃癌高、低发区健康居民血清微量元素分析

[目的]通过检测胃癌高、低发区健康居民血清中的微量元素含量,探讨微量元素与胃癌的关系.[方法]随机抽取高、低发区年龄在50～65岁范围的常住健康居民,高发区206人,低发区102人,抽取静脉血5 ml分离出血清,采用等离子体质谱法(ICP-MS)和原子荧光光谱法(AFS)检测镉(Cd)、锰(Mn)、钼(Mo)、汞(Hg)等16种微量元素,对数据进行非参数秩和检验.[结果]高发区健康居民血清中As、Ba、Cr、Hg、Li、Se、Fe、Zn 8种元素高于低发区,差异有统计学意义(P＜0.05);而Mo、Cd元素含量低于胃癌低发区,差异有统计学意义(P＜0.05).Co、Mn、Ni、Pb、Sr、Cu在两地健康居民血清中差异无统计学意义.[结论]胃癌高、低发区健康居民体内的微量元素负荷存在差异,钼、铁、铜、锌等元素可能与胃癌的发生有一定的关系.     

白族药野坝子的微量元素分析

用原子吸收分光光度法 (AAS)测定白族药野坝子的 Cu、Zn、Fe、Mn、Ca、Se、Ge、Ni、Co、Cd、Sr、Ba、Li、Mo、Si、Pb、Cr、Al的含量 ,发现野坝子中除含人体必需的多种微量元素外 ,还富含 Cu、Zn、Fe、Mn、Se,这为进一步研究和综合开发利用这一民族药提供了依据     

几种药食两用野菜中16种元素的含量测评

采用微波消解法处理马齿苋、苋菜和苦菜样品,电感耦合等离子体发射光谱(ICP-AES)测定其中K、Na、Ca、Mg、P、Sr、Fe、Mn、Zn、Cu、Co、Cr、Al、Ba、Ni和Cd 16种常量和微量元素的含量。16种元素的检出限为0.00003~0.00917μg/ml,回收率90.00%~107.85%,相对标准偏差(RSD)均小于5.0%。结果显示,马齿苋、苋菜和苦菜中除含有人体必需的常量元素K、Na、Mg、Ca外,还含有Fe、Zn、Mn、Cr、Co等必需的微量元素和其它元素P、Ni、Ba、Al、Sr、Cd、Ba等。     

ICP-OES和ICP-MS测定黄金茶中26种矿质元素含量

收集了2种不同产地的黄金茶样品,经过微波消解或密封消解后,采用电感耦合等离子体发射光谱法(ICP—OES)和电感耦合等离子体质谱法(ICP—MS)测定了其中Al,As,B,Ba,Ca,Cd,Co,Cr,Cs,Cu,Fe,K,Mg,Mn,Mo,Na,Ni,P,Pb,Rb,S,Sb,Se,Sr,V和Zn等26种矿质元素的含量,并用生物标准参考物质灌木枝叶组合样(GBW07603)评价分析了方法的准确度。研究结果表明:黄金茶中人体必需宏量元素K,Na,Ca,Mg,S,P和人体必需微量元素Zn,Cu,Fe,Mn等含量十分丰富,而有害元素Pb,Cd,As,Sb等含量极低。2种不同产地的黄金茶中一些元素的含量存在明显的差异,可能与地壳中化学元素分布的不均一性等因素有关。     

ICP—OES法同时测定中草药中18种微量元素

采用微波消解等离子体发射光谱法(ICP-OES)对10种中药中的18种微量元素Al、As、B、Ba、Ca、Cd、Cr、Cu、Fe、Mo、Mg、Mn、Na、Ni、P、Pb、Sr、Zn进行了测定。结果表明,除As、Cd、Mo、Pb外,各元素相对标准偏差(RSD)基本上小于5%;各元素的加标回收率在95%~106%之间。方法简便、快速、准确、可靠。     

201例血小板减少患者与正常人发检结果比较

血小板减少在各年龄段发病，系统性红斑狼疮患者有三分之一合并血小板减少，因此认为该病与免疫有关，但血小板减少真正病因尚不清。笔者用ICP法检验201例血小板减少患者头发中32元素含量与相同性别、相同年龄的正常人发检结果比较发现：La、Ce、Sb、Th、Al、Ge、Ga、Fe、Zr、Sc、Mo、Ti、Bi、B、Ba、Mn、Co、Li、Nb、V、Cr、Sr、Sn、Cd、Pb、Y等26种元素较正常人发中含量为高有相关性。笔者还检验癌症和系统性红斑狼疮患者合并血小板减少的76例与相同性别、年龄的正常人头发中32种元素含量，检验发现：La、Nb、Sb、Ge、Th、Mo、Ce、Ni、Fe、Ga、Bi、Al、Co、Sc、Mn、B、Li、Ba、Zr、Ti、V、Sn、Cd、Cr、Sr、Mg等26种元素较正常人发中含量为高有相关性。由此可见血小板减少多因子，即多种元素不平衡所致。血小板减少在现代医学属疑难病，但用元素平衡医学食疗法治疗血小板疗效特好，通常两个月治愈，最快的仅两个星期即可治愈。     

201例血小板减少患者与正常人发检结果比较

血小板减少在各年龄段发病，系统性红斑狼疮患者有三分之一合并血小板减少，因此认为该病与免疫有关，但血小板减少真正病因尚不清。笔者用ICP法检验201例血小板减少患者头发中32元素含量与相同性别、相同年龄的正常人发检结果比较发现：La、Ce、Sb、Th、A1、Ge、Ga、Fe、Zr、Sc、Mo、Ti、Bi、B、Ba、Mn、Co、Li、Nb、V、Cr、Sr、Sn、Cd、Pb、Y等26种元素较正常人发中含量为高有相关性。笔者还检验癌症和系统性红斑狼疮患者合并血小板减少的76例与相同性别、年龄的正常人头发中32种元素含量，检验发现：La、Nb、Sb、Ge、Th、Mo、Ce、Ni、Fe、Ga、Bi、Al、Co、Sc、Mn、B、Li、Ba、Zr、Ti、V、Sn、Cd、Cr、Sr、Mg等26种元素较正常人发中含量为高有相关性。由此可见血小板减少多因子，即多种元素不平衡所致。血小板减少在现代医学属疑难病，但用元素平衡医学食疗法治疗血小板疗效特好，通常两个月治愈，最快的仅两个星期即可治愈。     

电感耦合等离子体光谱法测定芜菁中微量元素的含量

目的:了解微量元素含量与中药药效的关系。建立一种电感耦合等离子体发射光谱法(ICP—AES)测定芜菁中微量元素含量的方法。方法:采用等离子体发射光谱法(ICP—AES)和原子吸收法测定了芜菁中微量元素的含量。结果:芜菁中含有Al,B,Ba,Be,Ca,Co,Cr,Cu,Fe,Li,Mg,Mn,Ni,P,Sr,Ti,V,Zn,Pb等19种元素。P,Mg,Fe,Sr,B,Cr,Zn,Mn等元素的含量较高。回收率为93.6%~106.0%。结论:等离子体发射光谱法(ICP—AES)具有检出限低、准确度高、线性范围宽的优点,可同时测定芜菁中的多种元素。     

全株丹参中多元素ICP-MS定量分析研究

目的 对药用植物丹参体内多种元素含量分布进行初步分析研究. 方法 样品经过消解后,In元素作内标,国家标准物质对照,以电感耦合等离子体质谱(ICP-MS)技术考察全株丹参中K、Na、Ca、Mg、As、Pb、Cd、Fe、Mn、Cu、Zn、Cr、Ba、Be、Co、Ni、V等47种元素. 结果定量检出的丹参元素中,K、Na、Ca、Mg、Fe、Mn、Cu、Zn、Al、B等含量较高,Hf、Ir、Pt、Au等未检出,丹参根茎叶中元素含量存在差异,根部Fe最丰富,As、Pb、Cd、Cu等含量均满足中国药典要求,通过国家标准物质对照与加标回收实验进行质量控制,结果满意,回收率85%～114%,线性范围0～150μg/g(r＞0.999),RSD(n=5)＜9%. 结论方法应用于植物药元素定量分析研究可行,简便快速,灵敏度高.     

脐带组织九种元素含量与胎龄的关系

目的探索胎龄与脐带组织中硒(Se)、钴(Co)、镉(Cd)、砷(As)、铅(Pb)、镍(Ni)、钼(Mo)、锌(Zn)、锰(Mn)元素含量的关系。方法采用电感耦合等离子体质谱(ICP-MS)检测2004年至2016年间因产前诊断引产或出生后诊断的166例神经管缺陷患儿脐带组织中Se、Co、Cd、As、Pb、Ni、Mo、Zn、Mn九种元素含量,元素含量采用中位值及四分位间距[M(P_(25),_(P75))]表示,采用有序多分类Logistic回归模型调整混杂因素后分析胎龄与元素含量的关系。结果脐带组织中Ni、Mo、Zn、Mn含量的中位值及四分位间距分别为28.99(20.10,49.76) ng/g、26.52(21.75,33.11) ng/g、52.69(44.79,61.78)μg/g、0.42(0.33,0.55)μg/g,且不同胎龄组的差异有统计学意义。以胎龄16～22周组为参照,Ni在38～44周组的OR值及95%CI为0.08(0.02-0.27);Mo在28～32周、38～44周组OR值及95%CI分别为2.91(1.06-8.02)、5.66(1.89-6.88);Zn在23～27周、33～37周组OR值及95%CI分别为0.32(0.13-0.76)、0.26(0.09-0.75);Mn在23～27周、28～32周、33～37周组OR值及95%CI分别为0.14(0.06-0.37)、0.28(0.10-0.79)和0.10(0.03-0.32)。脐带组织中Se、Co、Cd、As、Pb含量的中位值及四分位间距分别为0.56(0.50,0.64)μg/g、3.47(2.48,5.14) ng/g、1.33(0.57,3.51) ng/g、6.33(3.96,9.47) ng/g和27.32(18.37,50.34) ng/g,不同胎龄组间差异未见统计学意义。结论脐带组织中Zn、Mn、Mo、Ni量与胎龄有关,而Se、Co、Cd、As、Pb含量不随胎龄变化而改变。     

Determination of mineral contents of Turkish herbal tea (Salvia aucheri var. canescens) at different infusion periods

Inductively coupled plasma atomic emission spectrometry has been used for the determination of contents of major and minor elements in Salvia aucheri var. canescens (sage) and its infusions for different intervals. Twenty-five elements (Al, B, Ba, Bi, Ca, Cd, Co, Cr, Cu, Fe, Ga, Ln, K, Li, Mg, Mn, Na, Ni, P, Pb, S, Sr, Ti,V, and Zn) were detected in both S. aucheri and its infusions. The ranges of mineral levels varied from Cd (1.6668 mg/kg) to K (13,570 mg/kg) and Ni (0.05273 mg/kg) to K (196.25 mg/kg) in the herb and its infusions, respectively. The levels of K, Ca, Na, Mg, and S in all infusions in this work were higher than those of the other elements. However, Al, Ba, Ca, Cd, Co, Cu, Fe, Li, K, Mg, Na, Ni, P, Pb, Sr, Ti, and Zn contents were high in the first period of infusion. As a result, the first period of infusion may be proposed as the optimum infusion time because of nutritional properties. These results may be useful for the evaluation of dietary information.     

Assessment of reference values for selected elements in a healthy urban population

Reference values for 26 elements, namely Al, Ba, Be, Bi, Ca, Cd, Co, Cr, Cu, Fe, Hg, Li, Mg, Mn, Mo, Ni, Pb, Sb, Si, Sn, Sr, Tl, V, W, Zn and Zr are proposed in serum and blood of 110 healthy adults of the urban area of Rome. They were included in the study on the basis of strict criteria of eligibility and exclusion. With the exception of Ba, Bi, Co, Cr, Ni, Sb, Sn Tl in serum, and Bi, Hg, Si, V and W in whole blood, experimental data for each all the other analytes were found to approach a normal distribution. The estimated 5-95% references ranges (in ng ml(-1)) were reported. For several elements the reference ranges observed overlapped information available in the literature. Gender, age, body mass index, smoking habits and alcohol consume were used as grouping variables. Mutual associations were observed for several elements, as follows: Be, Ca, Co, Cr, Cu, Li, Mo, Pb and Zn with sex; Ca, Pb and Si with age (< and > 45 years); Co, Cr, Mo, Sb and Tl with body mass index; Cd and Pb with smoking habit; Cr and Pb with alcohol consume.     

Concentration of elements in serum of patients affected by multiple sclerosis with first demyelinating episode: a six-month longitudinal follow-up study

Twenty-six chemical elements and oxidative status were determined in serum of 12 patients with first demyelinating episode and brain magnetic resonance imaging compatible with the disease at different time points. Quantifications of Al, Ba, Be, Bi, Ca, Cd, Co, Cr, Cu, Fe, Hg, Li, Mg, Mn, Mo, Ni, Pb, Sb, Si, Sn, Sr, V, Tl, W, Zn and Zr, as well as of serum oxidative status and antioxidant capacity were carried out. The results were compared with values obtained from healthy subjects living in the same geographic area. Concentration variability, expressed as coefficient of variation (CV), was evaluated over a six months longitudinal follow-up. The CV was higher for Li and Pb, while showed minimal variation for Ca, Cu, Mg and Zn--elements strictly body regulated. Significant difference (p < or = 0.05) in mean concentrations of Ba, Ca, Cd, Cr, Li, Mn, Mo, Ni, Sb, Si, Sn and Zr between patients at time 0 and controls was also found.     

ICP-OES法分析18种中药材中11种元素及其分布特征

目的:采用电感耦合等离子原子发射光谱法研究18种中药材中11种无机元素的含量,寻找不同中药材中元素分布特性和相关性,为控制中药材中的有关元素含量、保障中药材质量和安全提供依据。方法:收集18种不同产地的中药材,经微波消解后,电感耦合等离子体原子发射光谱法(ICP-OES)同时测定铅、镉、钴、铬、镍、铜、锰、铁、锌、钡和铝元素含量,并应用SPSS 15.0软件对测定结果进行统计分析。结果:建立的ICP-OES法线性相关系数大于0.9999,相对标准偏差小于10%,加标回收率在80%～120%之间。药材中铝、铬、钴、镉、铜、铁、锰、镍、锌元素间存在相关性;铝、铬、铁、锰、镍、锌元素在部分药材品种中具有一定规律性和特异性;铝、钡、铁元素在某些药材中存在含量异常现象。结论:ICP-OES法简便快速、准确、可靠,是中药材中部分元素测定的一种较好方法,为从安全性和有效性方面控制药材质量提供了一种解决方案。     

Preliminary Health Risk Assessment of Potentially Toxic Metals in Surface Water of the Himalayan Rivers,Nepal

Bulletin of Environmental Contamination and Toxicology - This study investigates the contamination levels and risk assessments of 14 elements (Ba, Cd, Co, Cr, Cu, Pb, Li, Mn, Mo, Ni, Sb, Sr, V and...     

Comparison of element concentrations in fir and rhododendron leaves and twigs along an altitudinal gradient

Concentrations of 23 elements (Ca, K, Mg, P, Al, Cu, Fe, Mn, Mo, Na, Ni, Zn, Ag, Ba, Be, Cd, Co, Cr, Pb, Sb, Th, Tl, and V) in leaf and twig samples of a fir (Abies fabri) and a rhododendron (Rhododendron williamsianum) collected along an altitudinal gradient on Mount Gongga, China, are reported in the present study. Most of the macronutrients (K and P), micronutrients (Fe, Zn, Cu, Na, Ni, Mo, and Al), and trace elements (Pb, Tl, Ag, Cd, Ba, Co, V, Be, and Cr) are significantly enriched in fir when compared to rhododendron; however, Ca, Mg, Mn, Ba, and Cd are more enriched in rhododendron than in fir. Most of the elements in both plants are more significantly enriched in twigs than in leaves. The relationship between element concentration in plants and altitudinal gradient is nonlinear. Altitudes of 3,200 and 3,400 m are turning points for fir and rhododendron growth, respectively. Concentrations of all trace elements in the two plants along the altitudinal gradient are well below the toxic level in plants. No known industrial sources of the elements investigated exist in the Mount Gongga area, China. Element concentrations in the present study are higher than those found in mosses collected from the same area, indicating that the area is not contaminated. The element concentrations that we observed in plant samples were due to soil uptake. The pronounced differences between the two species are due to the different uptake characteristics of fir and rhododendron.     

频繁献血对21种元素代谢的影响

用ＩＣＰ－ＭＳ对３０例频繁献血者血清２１种元素水平进行测试分析，结果Ｂ、Ｃｒ、Ｉ、Ｓｅ、Ａｌ、Ｍｇ、Ｌｉ、Ｃｏ、Ｐ、Ｃｅ、Ｃａ、Ｌａ、Ｚｎ、Ｆｅ等１４种元素与对照组比较有显著差异，但Ｍｎ、Ｎｉ、Ｃｕ、Ｍｏ、Ｂａ、Ｃｄ、Ｓｒ等７种元素及性别、献血年限、献血总量、末次献血时间之间无明显差异。提示频繁献血不仅影响Ｆｅ代谢，也导致多种元素代谢紊乱。     

Trace element transfer from the mother to the newborn--investigations on triplets of colostrum, maternal and umbilical cord sera.

OBJECTIVE: To investigate the trace element transfer from the mother to the newborn. DESIGN: The concentrations of the eight essential elements calcium (Ca), cobalt (Co), copper (Cu), magnesium (Mg), manganese (Mn), molybdenum (Mo), tin (Sn), and zinc (Zn), and of the non-essential and toxic elements barium (Ba), beryllium (Be), bismuth (Bi), cadmium (Cd), cesium (Cs), lanthanum (La), lithium (Li), lead (Pb), rubidium (Rb), antimony (Sb), strontium (Sr), and thallium (Tl) were determined in umbilical cord (n = 29) and corresponding maternal sera (n = 29) as well as in colostrum (n = 27). RESULTS: Umbilical cord serum concentrations of Ca, Mn, and Zn were 120%, 150%, and 148% of the maternal value, respectively. Maternal sera had twice the Cu concentrations found in healthy adults and five-times higher Cu than umbilical cord sera. Concentration ratios colostrum/maternal serum and colostrum/umbilical cord serum were approximately one for Co, 1.4 for Mg, two for Ca, Mn, and Sn, five for Cu (maternal serum), eight for Mo, and ten for Zn. Concentrations of the toxic elements Cd and Pb decreased in the order colostrum (Pb 2.6 microg/L; Cd 0.6 microg/L), maternal sera (0.8 microg/L; 0.3 microg/L), umbilical cord sera (0.4 microg/L; 0.2 microg/L). Maternal serum Ba and Rb was 182% and 66% of the umbilical cord value. For Sr and Li, an almost perfect correlation between umbilical cord and maternal sera was found. For Ba, Co, Cu, Mn, Zn none, and for Ca, Cs, Mn, Mo, Rb only weak positive correlations between these two compartments could be established. CONCLUSIONS: The results of this study indicate that an active transport mechanism for the transport of Ca, Mn, Rb, and Zn from the mother to the newborn exists, whereas Cs, Li, and Sr follow concentration gradients. As regards Cu, the placenta showed to have a blocking effect on the transfer from the mother to the baby.     

Quantification of chemical elements in blood of patients affected by multiple sclerosis

Although some studies suggested a link between exposure to trace elements and development of multiple sclerosis (MS), clear information on their role in the aetiology of MS is still lacking. In this study the concentrations of Al, Ba, Be, Bi, Ca, Cd, Co, Cr, Cu, Fe, Hg, Li, Mg, Mn, Mo, Ni, Pb, Sb, Si, Sn, Sr, Tl, V, W, Zn and Zr were determined in the blood of 60 patients with MS and 60 controls. Quantifications were performed by inductively coupled plasma (ICP) atomic emission spectrometry and sector field ICP mass spectrometry. When the two groups were compared, an increased level of Co, Cu and Ni and a decrement of Be, Fe, Hg, Mg, Mo, Pb and Zn in blood of patients were observed. In addition, the discriminant analysis pointed out that Cu, Be, Hg, Co and Mo were able to discriminate between MS patients and controls (92.5% of cases correctly classified).