95例甲型H1N1流感患者危险因子及临床治疗分析

Objective To explore the curative effect of antivirus drugs to the patients with different degree A(H1N1) influenza, and to summarize the reasonable treatment protocols. Methods The clinical data of 95 adult patients with A( H1N1) influenza from June 2009 to February 2010 were collected and analyzed retrospectively. The differences in mild, severe and critical patients in physical status, hospital days, temperature peak, persistent fever period, oseltamivir treatment, other antiviral drugs and combined therapy were compared. Results There were statistical differences in risk factor (5.3%, 18.2%,66.1%, P<0.05), persistent fever period (2.2 days,5.6 days, 9.4 days, P<0.01), courses of treatment of oseltamivir were (4.1 ±0.4) days, (6.3 ±0.5) days, (9.2±1.8) days respectively ( P < 0.05). There were statistical differences among critical patients and mild, severe patients in mean onset days of oseltamivir oral(P<0.01). Cleaning time of virus in critical cases was longer than others cases. Comprehensive therapy included mechanical ventilation, antibiotics, corticoeteroid and blood plasma was necessary in severe patients. Conclusions The patients with critical high risks, especially with respiratory diseases or endocrine diseases, would progress to severe condition. The longer the fever, the easier to progress to severe condition. Oseltamivir is safe and effective, it is important to use it as early as possible. Oseltamivir should be used longer on the patients with severe condition, especially with respiratory disease. The comprehensive therapy is extremely important to severe patients.     

95例甲型H1N1流感患者危险因子及临床治疗分析

Objective To explore the curative effect of antivirus drugs to the patients with different degree A(H1N1) influenza, and to summarize the reasonable treatment protocols. Methods The clinical data of 95 adult patients with A( H1N1) influenza from June 2009 to February 2010 were collected and analyzed retrospectively. The differences in mild, severe and critical patients in physical status, hospital days, temperature peak, persistent fever period, oseltamivir treatment, other antiviral drugs and combined therapy were compared. Results There were statistical differences in risk factor (5.3%, 18.2%,66.1%, P<0.05), persistent fever period (2.2 days,5.6 days, 9.4 days, P<0.01), courses of treatment of oseltamivir were (4.1 ±0.4) days, (6.3 ±0.5) days, (9.2±1.8) days respectively ( P < 0.05). There were statistical differences among critical patients and mild, severe patients in mean onset days of oseltamivir oral(P<0.01). Cleaning time of virus in critical cases was longer than others cases. Comprehensive therapy included mechanical ventilation, antibiotics, corticoeteroid and blood plasma was necessary in severe patients. Conclusions The patients with critical high risks, especially with respiratory diseases or endocrine diseases, would progress to severe condition. The longer the fever, the easier to progress to severe condition. Oseltamivir is safe and effective, it is important to use it as early as possible. Oseltamivir should be used longer on the patients with severe condition, especially with respiratory disease. The comprehensive therapy is extremely important to severe patients.     

营养风险筛查2002的临床应用和分析

Objective To evaluate the clinical application of Nutritional Risk Screening 2002 (NRS 2002) in inpatients.Methods Totally 400 inpatients who were admitted to Tianjin Tianhe Hospital from Novem- ber 2008 to March 2009 were enrolled in this study.Physical examinations,including body height and body meas-urement,were performed the next morning after admission.The nutritional status was evaluated with NRS 2002.Results In all 400 inpatients.NRS 2002 was strongly practicable in 306 patients (76.5%) and weakly practica-ble in 94 patients (23.5%);Ninety-six patients (24.0%) had nutritional risks,which were most common in the department of internal medicine and the Department of neurology.The average age of patients with nutritional risks was (79.0±11.4) years,which was significantly higher than that of patients without nutritional risks [(58.1±15.8) years] (P<0.01).Conclusion NRS 2002 is effective and practicable in evaluating the nutritional status of inpatient.     

Caveolin-1过表达抑制宫颈鳞癌Hela细胞的体外生长研究

Objective To investigate the effects of caveolin-1 overexpressing on the growth of cervical squamous cell cancer Hela cell line. Methods Eukaryotic expression vector of human caveolin-1 gene was introduced into Hela cells by Lipofectamine. The clones stably overexpressed caveolin-1 were identiffed by RT-PCR, immunofluorescence cell staining techniques and Westernblotting. Cells proliferation viabihty was tested by MTT assay, and flow cytometry was used to assay the cell cycle and apoptosis, and the relative phosphorylation level of extracellular regulated protein kinases (Erk1/2) were detected by Westernblotting. Results The clones stably overexpressed caveolin-1 were obtained. Compared with the parental Hela cells, the tranfected cells exhibited a slower rate of growth. FAGS analysis results revealed that overexpression of caveolin-1 resulted in the cell cycle arrest in the G0/G1 [ ( 68. 04 ± 2. 57 ) % vs ( 53.41 ±1.01)%] phase and increased the apoptotic cell fraction[ (19. 18 ±2.20)% vs (5.63 ±0.55)%, P <0. 05 ]. Western blotting results showed that overexpression of caveolin-1 reduced the phosphorylation of Erk1/2(0.28 ±0.05 vs 0.81 ±0.07, P <0.05). Conclusions Overexpression of caveolin-1 suppressed the growth of Hela cells and induced apoptosis, down-regulation of Erk1/2 phosphorylation might be involved in its mechanism.     

结晶型硫化镍诱发细胞恶性转化过程中基因组总体DNA甲基化的改变

Objective To observe the effect of crystalline NiS on genome DNA methylation profile in in vitro cultured cells.Methods 16HBE Cells were treated with crystalline NiS at 0.25,0.50,1.00 and 2.00 μg/cm2 for 24 h and three times at total.DAC treatment was given at 3 μmol/L for 72 h.5-mC immunofluorescence and SssI emthyltrasferase assay methods were applied to investigate if the hypomethylation of genome DNA involved.Results The results of 5-mC immunofluorescence showed that the fluorescence intensity of NiS-treated cells were decreased in some degree, and transformed cells were decreased dramatically.By the SssI methylase assay, an average of (81.9 ± 7.3 )% methylated CpG were found in negative control cells.By contrast, ( 77.9 ± 6.2) %, ( 75.3 ± 6.8 ) %, ( 59.5 ± 4.9 ) %, ( 67.4 ±5.1 ) % methylated CpG were observed in cells treated with NiS for three times at dosage of 0.25,0.50,1.00and 2.00 μg/cm2 which were abbreviated as NiS0.25, NiS0.50, NiS1.00, NiS2.00 respectively.The ANOVA analysis results showed that there was a significant difference in the 5 groups above ( F = 124.95,P ＜0.01 ).The results of Dunnett-t test showed that the methylated CpG of both group NiS1.00 and NiS2.00were significantly decreased compared with the negative control group(t values were 7.64,4.89 respectively,P ＜0.01 ).For methylated CpG, (46.2 ±4.1 ) % and (43.6% ±4.3)% were observed in NiS-transformed cells (NSTC1 and NSTC2) which were dramatically decreased compared with the negative control group(t values were 12.79,13.56 respectively, P ＜ 0.01 ).Conclusion Genomic DNA methylation levels were decreased during NiS induced malignant transformation.     

结晶型硫化镍诱发细胞恶性转化过程中基因组总体DNA甲基化的改变

Objective To observe the effect of crystalline NiS on genome DNA methylation profile in in vitro cultured cells.Methods 16HBE Cells were treated with crystalline NiS at 0.25,0.50,1.00 and 2.00 μg/cm2 for 24 h and three times at total.DAC treatment was given at 3 μmol/L for 72 h.5-mC immunofluorescence and SssI emthyltrasferase assay methods were applied to investigate if the hypomethylation of genome DNA involved.Results The results of 5-mC immunofluorescence showed that the fluorescence intensity of NiS-treated cells were decreased in some degree, and transformed cells were decreased dramatically.By the SssI methylase assay, an average of (81.9 ± 7.3 )% methylated CpG were found in negative control cells.By contrast, ( 77.9 ± 6.2) %, ( 75.3 ± 6.8 ) %, ( 59.5 ± 4.9 ) %, ( 67.4 ±5.1 ) % methylated CpG were observed in cells treated with NiS for three times at dosage of 0.25,0.50,1.00and 2.00 μg/cm2 which were abbreviated as NiS0.25, NiS0.50, NiS1.00, NiS2.00 respectively.The ANOVA analysis results showed that there was a significant difference in the 5 groups above ( F = 124.95,P ＜0.01 ).The results of Dunnett-t test showed that the methylated CpG of both group NiS1.00 and NiS2.00were significantly decreased compared with the negative control group(t values were 7.64,4.89 respectively,P ＜0.01 ).For methylated CpG, (46.2 ±4.1 ) % and (43.6% ±4.3)% were observed in NiS-transformed cells (NSTC1 and NSTC2) which were dramatically decreased compared with the negative control group(t values were 12.79,13.56 respectively, P ＜ 0.01 ).Conclusion Genomic DNA methylation levels were decreased during NiS induced malignant transformation.     

Change of circulating Th1 and Th2 cells in chronic hepatitis B patients after pegylated interferon α-2a treatment

Objective To investigate the feature of Th1 and Th2 cells in chronic hepatitis B(CHB) patients complete responsed after pegylated interferon α-2a treatment. Methods Serum and PBMC were obtained from 7 enrolled CHB patients, 9 health controls and 9 untreated hepatitis B patients, and monitored for ALT, HBV DNA, HBV markers as well as the frequency of CD3+ CD4+ IFN-γ+ T cells, CD3+ CD4+ IL-4+ T cells. Results All the 7 patients occured HBsAg conversion, the short est conversion time was 36 weeks, and the long est conversion time was 49 weeks, the average time was(39.28 ± 6.44)weeks. The frequency of CD3+CD4+IFN-γ+T cells in 7 completely responsed patients decreased gradually, but it was still higher than the health control group in followed 6 months; the frequency of CD3+CD4+IL-4+T cells increased in whole response time point but still lower than the health control group and decreased in followed 6 month. Conclusions In patients with whole response to pegylated interferon α-2a, Th1 immune reaction decreases and Th2 immune reaction is insufficient which correlates with the hepatic inflammation.     

血液灌流结合连续性静脉-静脉血液滤过对急性百草枯中毒疗效的研究

Objective To explore the therapic effects of hemoperfusion (HP) with continuous venovenous hemofiltration (CVVH) on the patients with acute paraquat poisoning. Methods Nighty-one patients with acute paraquat poisoning were randomly divided into HP group (49 cases) and HP-CVVH group(42 cases). The mortality, survival duration and the death causes between the two groups were compared and analyzed. Results There were no significant differences in mortality (59.2% versus 61.9%) between the two groups. The mean time between poisoning and death in HP-CVVH group was (4.9±3.1) days, which was significantly longer than that (3.5 ±2.0) days in HP group (P＜0.05).The death proportion on 4th day after poisoning in HP group was 62.1%(18/29), which was significantly higher than that (30.8%, 8/26) in HPCVVH group (P＜0.05). The hypoxia appeared in 4.3±2.5 days after poisoning in HP-CVVH group, which was significantly longer than that (3.2±1.9) days in HP group (P＜0.05). The mortality due to respiratory failure in HP group was 20.4%(10/49),which was significantly lower than that (40.5%, 17/42) in HP-CVVH group (P＜0.05). The incidence of acute renal failure in HP group was 63.3%(31/49), which was significantly higher than that (40.5%,17/42) in HP-CVYH group (P＜0.05). Conclusion The combined therapy of HP and CVVH can prevent the patients with acute paraquat poisoning from early death and prolong the survival duration, but can not reduce mortality for the patients with acute paraquat poisoning.     

三氧化二砷联用华蟾素对K562细胞增殖和凋亡的影响

Objective To investigate the effect of As2 O3 in combination with cinobufacini on proliferation and apoptosis of the K562 cells and provide theoretical basis for clinical application.Methods Cell proliferation was assayed by analyzing the growth and viability of the cells.Apoptosis was assayed by performing cell morphology,Annexin-V/PI staining,DNA-PI staining,and DNA gel electrophoresis.Results After exposure to As2O3 and cinobufacini,the growth of K562 cells was inhibited and the viability of K562 cells was decreased. After treated with 1.0μmol/L As2O3,0.125μg/ml cinobufacini,0.25μg/ml cinobufacini,1.0μmol/L As2O3 + 0.125 μg/ml cinobufacini,1.0μmol/L As2O3 + 0.25μg/ml cinobufacini for 24 and 48 hours,the proliferation inhibition rate were(24 ± 1.3)%,(21 ± 1.5)%,(38 ± 3.1)%,(57 ±2.7)%,(66 ±3.3)% and(49 ±2.9)%,(48 ±2.7)%,(61 ±2.1)%,(77 ±3.8)%,(82 ±4.2)%,the apoptosis rate of K562 cells were(4.8 ± 0.5)%,(5.6 ± 0.7)%,(9.8 ± 0.6)%,(11.9 ± 1.2)%,(15.2±1.5)% and(11.0 ±0.9)%,(12.9 ±1.1)%,(18.4 ±1.5)%,(21.0 ±2.0)%,(28.0 ±1.9)%.The percentage of apoptotic cells was a time- and dose-dependent manner.Typical DNA ladder was shown by DNA gel electrophoresis.Conclusions As2O3 combined with cinobufacini inhibited the proliferation of K562 cells and induced apoptosis of the K562 cells,the combination of the two drugs had better effect.     

慢性乙型肝炎患者血清XCL1与免疫损伤的相关性分析

Objective To explore the relevance between serum XCL1 levels and liver damage in hepatitis B patients.Methods The serum concentration of XCL1 was detected by enzyme linked immu-nosorbent assay (ELISA).Peripheral blood T-cell subsets were detected by flow cytometry (FCM).Liver function was assayed by automatic biochemistry analyzer, hepatitis B antigen/antibody semi-quantitative index was detected by time-resolved fluorescence analyzer, and HBV-DNA load was detected by automatic fluorescence quantitative PCR.Results Serum concentration of XCL1 in control group ( n = 20), mild chronic hepatitis B (CHB) group ( n =29), moderate CHB group ( n =20) and severe CHB group ( n =26)were (8.24±1.94) pg/ml, (10.99±1.94) pg/ml, (12.83 ±2.59) pg/ml, (13.72 ±3.13) pg/ml,respectively.The concentration of XCL1 in all CHB groups was significantly higher than control group ( P＜ 0.05 ).The concentration of XCL1 in severe CHB group was significantly higher than mild CHB group (P ＜ 0.05).XCL1 was positively correlated with ALT, AST, TBIL and DBIL, and the coefficients were (r =0.463、 0.472、 0.413、 0.440, P ＜0.01 ), respectively.The serum XCL1 levels in hepatitis B virus with low load group was lower than hepatitis B virus with high load group.The percentage of CD4 + T in hepatitis B virus with low load group and high load group were (41.26 ± 11.33)%, (33.01 ± 5.96)%,and the difference was statistically significant.Conclusion Serum concentrations of XCL1 were closely related to the degree of liver inflammation in hepatitis B patients.XCL1 may be involved in the process of chronic hepatitis B.     

CYP1A1、GSTT1、GSTM1基因与乳腺癌易感性的病例对照研究

目的探讨雌激素代谢通路相关基因CYP1A1、GSTT1、GSTM1与乳腺癌易感性的关系。方法采用聚合酶链反应(PCR)、限制性片段长度多态性(RFLP)及琼脂糖凝胶电泳法,对天津市360例正常女性和315例女性乳腺癌患者的CYP1A1、GSTT1、GSTM1基因及多态性进行检测,Logistic回归分析评估单基因、联合基因以及相关因素对乳腺癌的危险度。结果 CYP1A1基因、GSTT1基因、GSTM1基因在两组间分布频率有差异(χ2值分别为20.677,47.250,43.621,P=0.000)。基因型联合分析显示,随着携带危险基因型数目的增加,个体罹患乳腺癌的危险性增加(χ2=51.366,P=0.000)。多因素非条件Logistic回归分析结果显示,CYP1A1基因与体育锻炼、摄入肉类(每日多于150g)、摄入蔬菜(每日超过300g)的交互作用有统计学意义[OR(95%CI)分别为0.465(0.362～0.597)、1.559(1.344～1.808)、0.465(0.362～0.597)];GSTT1基因缺失、GSTM1基因缺失是乳腺癌的危险因素[OR(95%CI)分别为3.245(1.645～6.375)、2.462(1.818～3.334)],且GSTT1基因与体育锻炼、累计行经年数的交互作用有统计学意义[OR(95%CI)分别为1.546(1.113～2.147)、3.735(1.401～9.956)],GSTM1基因与哺乳期限、绝育手术的交互作用有统计学意义[OR(95%CI)分别为1.206(1.024～1.420)、1.690(1.353～2.111)]。结论雌激素代谢通路相关基因与乳腺癌发生有关,且其与雌激素暴露影响因素、生活方式等存在交互作用。     

母亲和新生儿GSTM1、GSTT1和细胞色素P_(450)1A1基因多态性与早产易感性关系

目的探讨母亲和新生儿GSTM1、GSTT1基因以及细胞色素P4501A1基因多态性对早产的影响。方法采用病例-对照调查方法,应用多重PCR和限制性内切酶PCR(RFLP-PCR)技术对早产母亲和对照母亲以及早产新生儿和对照新生儿GSTM1、GSTT1基因和CYP1A1基因MSPI位点多态性分别进行检测。结果GSTM1、GSTT1基因和CYP1A1基因MSPI位点多态性在早产母亲和对照母亲组中没有显著性差异(P>0.05);以上基因在早产新生儿和对照新生儿组中也没有显著性差异(P>0.05)。GSTM1与GSTT1联合基因型在母亲和新生儿的病例与对照组中也没有显著性差异(P>0.05);GSTT1缺失型和CYP1A1变异型联合基因在早产母亲组与对照母亲组中有显著性差异(χ2=4.683,P<0.05,OR=2.440)。结论携带GSTT1缺失型基因以及CYP1A1变异型基因的母亲,其发生早产的危险性增大。     

Niemann-Pick C1 Like 1研究新进展

Niemann-Pick C1 Like 1(NPC1L1)是肠道胆固醇吸收的关键蛋白,也是胆固醇吸收抑制剂依泽替米贝的分子作用靶点.NPC1L1的表达受一些核因子受体调控.敲除apoE-/-小鼠NPC1L1基因能显著抑制动脉粥样硬化的发生和发展,为动脉粥样硬化和冠状动脉性心脏病提供了新的治疗靶点.     

环境外暴露及GSTT_1、GSTM_1基因型对1-羟基芘作为多环芳烃暴露标志物的影响

目的研究多环芳烃(PAHs)暴露(环境空气暴露和吸烟)、GSTT1、GSTM1基因型对1-羟基芘(1-OHP)作为PAHs暴露标志物的影响。方法选取51名巡警作为研究组,48名内勤警察作为对照组,测定两组人群环境空气中的PAHS浓度以及尿中的1-OHP浓度,采用HPLC方法分析环境空气中PAHs浓度和研究对象尿样中的1-OHP;PCR方法测定GSTT1、GSTM1基因型。结果对照组环境PAHs浓度为12.79ng/m3,研究组为20.85ng/m3。研究组、对照组内部相同吸烟条件下GSTT1、GSTM1不同基因型人群尿中1-OHP浓度没有差别,相同基因型下的非吸烟人群中,对照组尿中1-OHP浓度均小于研究组,吸烟人群中没有发现同样的规律。按吸烟分层后,研究组、对照组内部吸烟者尿中的1-OHP浓度均大于非吸烟者,并且对照组吸烟者尿中1-OHP浓度大于研究组非吸烟者。结论PAHs暴露及吸烟是影响尿中1-OHP浓度的重要因素;在环境空气中PAHs浓度较低的情况下,吸烟对尿中1-OHP浓度的贡献更大。但GSTT1、GSTM1基因型不是影响尿中1-OHP浓度的主要因素。     

铜川市甲型流感监测流行情况

目的通过甲型流感监测,探索铜川市2009年9月-2010年1月该病流行趋势。方法实时荧光定量PCR方法 (Real-timePCR)。结果共监测476份样本,阳性184份,阳性率38.7%。结论 184份阳性标本中,甲型H1N1阳性样140份,阳性率76.1%。     

CYP1A1和GSTM1基因多态与肺癌发病关系的病例-对照研究

目的 探讨肺癌易感性标记物CYP1A1及GSTM1基因多态以及吸烟等其他环境暴露因素与肺癌发生的关系。方法 采用病例-对照研究的方法，收集原发性肺癌病例91例以及非肺部疾患的住院病例(对照)91例，所有的研究对象均采静脉血进行DNA抽提，并用PCR方法检测CYP1A1以及GSTM1基因多态，同时调查研究对象吸烟等其他环境暴露因素。应用Logistic回归分析方法进行单因素和多因素的分析。结果 无论是单因素分析还是多因素分析均未显示出CYP1A1和GSTM1基因多态与肺癌发病的关联。多因素分析结果表明：化程度的OR为0．63(95％CI：0．45～0．86)，吸烟量的OR为1．56(95％CI：1．14～2．14)，无抽油烟机的OR为3．77(95％CI：1．48～9．56)，食用动物油的OR为1．67(95％CI：1.25～2．24)，常吃胡萝卜的OR为0．47(95％CI：0．22～0．98)，饮酒的OR为6．58(95％CI：1.53～28．30)，家族肺癌史的OR为3．75(95％CI：1．64～8．58)。结论 CYP1A1和GSTM1基因多态与肺癌发病无明显的关联，吸烟、饮酒、食用动物油、家族肺癌吏以及无抽油烟机是肺癌的危险因素，而高化程度和常吃胡萝卜与降低肺癌风险有关。     

1起甲型H1N1流感暴发的调查

[目的]总结甲型H1N1流感暴发的经验、教训,为今后处理类似突发事件提供参考。[方法]采用现况调查的方法,对2009年10月发生在青岛某大学费县校区学生中的甲型H1N1流感暴发疫情进行调查。[结果]此次甲型H1N1流感暴发共发病16例,罹患率为0.28%。病例分布在2个年级、9个班,发病年龄为18-21岁,男生11例,女生5例,男女之比为2.20∶1。患者临床表现为发热、头痛、头晕、咳嗽、腰腿酸痛等症状。无明确与甲型H1N1流感病例和流感样病例接触史。[结论]这是1起发生在学校由甲型H1N1流感病毒感染引起的暴发疫情,控制措施及时有效。     

硫酸基转移酶SULT1E1、SULT1A1基因多态性与子宫平滑肌瘤易感性的关联研究

目的:探讨硫酸基转移酶SULT1E1、SULT1A1基因多态性对子宫平滑肌瘤易感性的影响。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析法检测子宫平滑肌瘤组和对照组SULT1E1基因rs3736599位点、SULT1A1基因rs9282861位点的多态性情况。结果:①病例组和对照组SULT1E1 rs3736599位点基因型分布差异有统计学意义(P=0.032),携带突变A等位基因(基因型为A/A和A/G)女性发生子宫平滑肌瘤的风险是野生型纯合子G/G女性的3.497倍(P=0.034,OR=3.497,95%CI:1.12～10.91)。②病例组和对照组SULT1A1 rs9282861位点基因型分布差异无统计学意义。结论:硫酸基转移酶SULT1E1基因rs3736599多态性可能与子宫平滑肌瘤易感性相关,携带突变A等位基因可能是子宫平滑肌瘤的危险因素。     

Detection of CYP1A1 gene polymorphism using designed RFLP and distributions of CYP1A1 genotypes in Japanese

Isoleucine (Ile)-valine (Val) polymorphism, which is caused by a point mutation from A to G in exon 7, is reported to be associated with an elevated risk of lung cancer among Japanese. Because CYP1A1 catalyzes bioactivation of environmental procarcinogens, such as benzo[a]pyrene, it is very important to study the clinical meaning of Ile-Val polymorphism using an epidemiological study. In an epidemiological study, easy, economical, rapid and reliable identification of the CYP1A1 genotype is necessary. The present study shows that the new method, designed restriction fragment length polymorphism (designed RFLP), can detect Ile-Val polymorphism of CYP1A1 The Ile-Val polymorphism detected using this new method was consistent with that found by the allele-specific PCR amplifications (ASA) method in six cases tested. This new method detected Ile-Va1 polymorphism of CYP1A1 using 240 healthy Japanese who lived in the northern Kyusyu region. The frequency of the genotypes was as follows: Ile/Ile, 159 (66.2%); Ile/Val, 65 (27.1%); Val/Val, 16 (6.7%). The frequency of the Ile gene was 0.798 and that of the Val gene, 0.202. There was no difference in Ile-Val polymorphism based on sex or age. Racial differences influenced the distribution of this polymorphism, but Japanese regional differences did not. Since this new method, designed RFLP, is rapid, reliable and suitable for large-scale screening of polymorphisms, it may be used routinely to detect Ile-Val polymorphism of CYP1A1 Furthermore, it will help to evaluate the relationship between CYP1A1 polymorphism and individual sensitivity to xenobiotics that may affect the incidence of lung cancer.