草分枝杆菌F．U．36治疗支气管哮喘临床观察

目的：评价草分枝杆菌F.U．36辅助治疗支气管哮喘的疗效。方法：38例支气管哮喘患者随机分为治疗组和对照组，两组基本条件大致相同。其中治疗组加用草分杖杆菌F.U．36，其它治疗两组完全一致。结果：治疗组和对照组总有效率分别为94．7％及63．1％，两组比较差异显著（P〈0．05）。结论：草分枝杆菌F．U．36辅助治疗支气管哮喘，疗效得到明显提高。     

异位性皮炎的最新治疗进展

异位性皮炎（AD）是一种以皮肤瘙痒、皮疹多形性为特征的一种慢性炎症性疾病，其发病机制还不太明确，因此，在治疗上尚无可完全根治的特效方法。但随着对其免疫调节功能的研究，一些新的治疗方法也逐渐应用于临床，本文对现有的治疗方法进行总结归纳如下，希望能对临床治疗提供参考和借鉴。     

草分枝杆菌联合阿昔洛韦治疗扁平疣疗效观察

目的：观察草分枝杆菌联合阿昔洛韦治疗扁平疣的临床疗效。方法：将132例扁平疣患者随机分为治疗组78例，口服阿昔洛韦联合草分枝杆菌注射液肌内注射；对照组54例，仅口服阿昔洛韦，疗程均为1个月。结果：治疗组有效率85．90％，对照组50％，两组比较差异有统计学意义（P〈0．01）。结论：分草枝杆菌注射液联合阿昔洛韦治疗扁平疣疗效满意。     

皮安诺肤痒冲剂治疗荨麻疹湿疹及异位性皮炎疗效观察

荨麻疹、湿疹及异位性皮炎是皮肤科常见病，两年来我科应用皮安诺肤痒冲剂治疗荨麻疹、湿疹及异位性皮炎222例，收到满意效果，并与应用赛庚啶治疗进行了对照观察，报告如下：     

草分枝杆菌注射液辅助治疗老年复治性肺结核的临床研究

老年复治性肺结核是指年龄65岁以上疾病迁延不愈或65岁以后复发的患者。在此之前曾服用抗结核药物,特别是不规则用药者,使结核菌对部分抗结核药物产生耐药性,疗效差。鉴于肺结核的发生、发展及转归与机体的免疫状态密切相关,草分枝杆菌注射液具有免疫调节功能,我们采用草分枝杆菌注射液联合抗结核药物治疗老年复治性肺结核60例,取得良好效果,现报告如下。1资料与方法1．1病例选择2005年3月至2007年9月,河北省胸科医院收治的复治性肺结核患者,年龄均在65岁以上,在院外曾规律或不规律服用异烟肼、     

特应性皮炎的免疫学发病因素研究进展

特应性皮炎（AD）,也称“异位性皮炎”、“遗传过敏性皮炎”,是一种与遗传过敏素质相关的复发性、慢性炎症性皮肤病,表现为剧烈瘙痒、皮肤干燥、慢性湿疹样皮炎,常伴发哮喘、过敏性鼻炎。     

他克莫司治疗异位性皮炎的研究进展

异位性皮炎(AD)是一种常见的慢性瘙痒性皮肤病，具有遗传易感性.患者的年龄通常在30岁以下，大多数自幼年发病。在发达国家，超过20％的儿童均被确认曾罹患该病。尽管多数患者随着年龄增长病情会逐渐改善，但仍有近60％的患者出现皮肤干燥、苔藓样变、刺激性接触反应。治疗的目的主要是减轻炎症、缓解症状。一般为口服抗组胺药，局部使用润肤剂和皮质类固醇激素等。自1952年以来，     

母牛分枝杆菌菌苗辅助治疗耐多药肺结核疗效观察

目的 观察和评价母牛分枝杆菌菌苗（微卡）在治疗耐多药结核病病中的效果。方法随机将58例耐多药肺结核患者分为治疗组（29例）和对照组（29例）。根据患者既往用药史及药敏报告，选择未使用药物及敏感药物组合化疗方案。2组均采用统一结核病化疗方案：3LivTHAK／15LivTH，治疗组肌肉注射母牛分枝杆菌菌苗22．5μg，强化期每10天1次，继续治疗期前3个月，15d／次，后12个月，1月／次，共用21次。对照组不用母牛分枝杆菌菌苗。观察2组痰菌阴转、病灶吸收、空洞闭合情况。结果治疗3、6、9、18个月末痰菌阴转率治疗组为58．6％、68．9％、82．7％、96．6％；对照组为31．O％、41．3％、55．2％、72．4％（P〈0．05）；疗程结束时治疗组和对照组胸部x线病灶吸收率分别为93．1％和68．9％（P〈0．05）；空洞闭合率分别为92．6％和65．4％（P〈0．05）。随访治疗组无1例复发，对照组的细菌复发率为9．5％（P〉0．05）。结论母牛分枝杆菌菌苗联合其他化疗药物治疗耐多药结核病，能加快痰菌转阴、病灶吸收和空洞闭合的速度，随访一年无1例复发，不良反应少且较轻微。母牛分枝杆菌菌苗是非常有效的耐多药肺结核患者免疫治疗剂。     

草分子杆菌F·U·36对支气管扩张症临床疗效观察

目的:探讨草分子杆菌F·U·36对中重度支气管扩张症患者的临床疗效.方法:将95例中重度支气管扩张症患者随机分为治疗A组、治疗B组及对照组,对照组在伴发感染时予抗感染、平喘等治疗,治疗A组和治疗B组在此基础上分别加用草分子杆菌F·U·36针3.44 μg和卡介菌多糖核酸针0.7 mg肌肉注射,每周3次,共12周.3组均观察半年,评价治疗前后肺功能FVC、FEV1,血清IgG、IgM、IgA水平以及呼吸道再发感染情况的变化.结果:治疗半年后治疗A组血清IgG、IgM水平较其他两组有明显提高(P<0.05),且呼吸道再发感染的次数和持续时间均明显减少(P<0.05).结论:草分子杆菌F·U·36能通过调节患者机体免疫功能,有效改善中重度支气管扩张症患者的预后     

乌体林斯联合顺铂治疗恶性胸腔积液的临床研究

目的研究乌体林斯联合顺铂治疗恶性胸腔积液的临床疗效.方法将64例恶性胸水患者随机分为治疗组和对照组各32例,治疗组用乌体林斯加顺铂胸腔内注射,对照组单用顺铂胸腔内注射,观察治疗前后的近期疗效、活动状况、外周血免疫指标变化和不良反应.结果治疗组的近期疗效、活动状况改善程度均明显优于对照组(P<0.01);治疗组治疗后的CD3、CD4、CD4/CD8及自然杀伤细胞活性均高于治疗前(P<0.05),对照组免疫指标治疗前后无明显变化,治疗后两组免疫指标比较差异有统计学意义(P<0.05);治疗组不良反应仅为发热、胸痛,而对照组出现骨髓抑制、胃肠道反应和胸痛. 结论乌体林斯联合顺铂胸腔注射治疗恶性胸水有较好的临床疗效.     

乌体林斯对慢性阻塞性肺病患者免疫功能的影响

【目的】研究乌体林斯注射液对慢性阻塞性肺病（COPD）稳定期患者免疫功能的影响及,临床意义。【方法】将COPD稳定期患者随机分为乌体林斯治疗组和对照组,对比两组患者用药前后急性加重的发病频率、住院次数及血清CD3＋、CD4＋、CD8＋及CD4＋／CD8＋的变化。【结果】乌体林斯治疗组较对照组患者急性加重发病频率、住院次数明显减少,治疗组用药前后比较血清CD4＋及CD4＋／CD8＋水平有明显增加;和对照组比较亦有明显增加。【结论】乌体林斯能提高COPD患者的免疫功能,减少COPD急性加重频率,减少住院次数,提高生活质量,是一种良好的免疫调节剂,值得临床推广使用。     

草分支杆菌F.U.36混悬注射液对人脐血树突状细胞体外扩增的影响

为了探讨草分支杆菌F．U．36混悬注射液（鸟体林斯,U）对人脐血来源树突状细胞（DC）体外扩增有无影响,应用Ficou-Hypaque法分离人脐血单个核细胞（CB-MNC）,对照组以RPMI1640培养液诱导培养CB．MNC。实验组分为Utilin“s”组（仅加入Utilin“s”PRMI1640）,GTI组（GM-CSF,TNF-α,IL-4）和GTIU组（GM-CSF。TNF-α IL-4和Utilin“s”）。在光学显微镜下观察DC生长情况。至培养第10天,应用流式细胞仪检测各组细胞免疫表型,并将细胞涂片行瑞氏-姬姆萨染液染色,在油镜下观察摄片。结果显示：3个实验组均得到一定数量的典型DC;Utilin“s”组CDIa^＋、HLA-DR^＋细胞比例高于对照组;GTIU组HLA-DR’细胞比例升高最明显,高于GTI组。结论：草分支杆菌F．U．36混悬注射液不仅能促进脐血DC体外扩增。还能协同rhGM—CSF、rhTNF-α、rhIL4促进DC成熟。     

益生菌对小年龄儿童湿疹治疗SCORAD指数的Meta分析

目的利用Meta分析评价益生菌对小年龄儿童湿疹的治疗效果。方法按照系统评价的要求全面检索PubMed、Medline、EMBASE等文献数据库,根据临床和统计标准采用Cochrane协作网提供的Rev-Man 5.2软件进行合并分析。结果共检索到85篇文献,符合纳入标准的有5项临床随机对照研究,共计528例湿疹病例进入Meta分析。纳入分析的5项研究的异质性检验具有临床和统计学上的同质性（ P＝0.18,I2＝36％）,用固定效应模型进行Meta分析,结果显示益生菌对小年龄儿童湿疹治疗前后SCORAD指数均数的合并统计值及95％的可信区间为1.71（1.17～2.24）,与对照组相比,添加益生菌并不能明显降低湿疹患儿的SCORAD指数。结论益生菌治疗小年龄儿童湿疹无明显疗效。     

核糖体蛋白RPL36A siRNA对U937细胞的促增殖、抑凋亡作用

本研究探讨RPL36A基因在急性髓系白血病(AML)病人的表达情况及可能的作用机制。应用RT-PCR检测急性髓系白血病细胞、正常人单个核细胞、U937细胞中RPL36A基因的表达情况;RPL36A siRNA经脂质体介导转入U937细胞,采用MTT法及DNA倍体分析检测细胞增殖,AO/EB、TUNEL、Annexin V/FITV检测细胞凋亡,RT-PCR和Western blot检测RPL36A基因及蛋白表达水平的变化。结果表明:RPL36A在初治AML细胞和U937细胞中表达明显增高;MTT法及DNA倍体分析发现,U937细胞增殖受到抑制,细胞周期阻滞;AO/EB染色、TUNEL、Annexin V/FITV检测显示,转染RPL36A siRNA的细胞组凋亡率高于对照组;RPL36A siRNA作用可引起U937细胞的RPL36A的mRNA水平的降低和蛋白表达量的下降,且呈时间依赖性(r分别为0.9813和0.9537)。结论:AML细胞高表达RPL36A基因,RPL36A基因的高表达可能促进AML细胞的过度增殖并抑制其凋亡。     

Comparison of the effect of GIP and GLP-1 (7–36amide) on insulin release from rat pancreatic islets

After ingestion of glucose both GIP (gastric inhibitory polypeptide, glucose-dependent insulinotropic polypeptide) and GLP-1(7-36amide) (glucagon-like polypeptide-1, 7-36amide) may play a physiological role in augmenting insulin release. Their insulinotropic effect was compared in isolated rat islets after 24-h maintenance in tissue culture (11 mmol l-1 glucose). Ten islets per vial were then incubated in Krebs-Ringer-Hepes buffer for 30 min; insulin was measured radioimmunologically. Both hormones were always compared in the same experiment. At 16.7 mmol l-1 glucose both GIP and GLP-1(7-36amide) 2 x 10(-10) mol l-1 significantly increased insulin release; 10(-10) mol l-1 of either hormone had no significant effect. The response at 10(-9) and 10(-8) mol l-1 was similar for both; at 4 x 10(-10) mol l-1 GLP-1(7-36amide), however, was clearly more effective than GIP. At low glucose (2.8 or 5.0 mol l-1) no significant differences were found. A concentration of 10(-8) mol l-1 of both hormones was slightly stimulatory. At 8.3 mmol l-1 glucose, 10(-9) mol l-1 GLP-1(7-36amide) was 60% more effective than GIP (4.8 +/- 0.4 vs. 3.0 +/- 0.4, n = 13, P less than 0.005), the response to 10(-8) mol l-1 was similar. These data show comparable effects of high concentrations of GIP and GLP-1(7-36amide) on glucose-induced insulin release; at presumably physiological concentrations, however, GLP-1(7-36amide) was clearly more effective. The combination of the two peptides was not more than additive, suggesting that they act via the same final mechanism.     

The effect of haemodialysis on cerebrospinal fluid and plasma amino acids.

The concentration of free amino acids in CSF and plasma has been measured in 19 patients with renal disease undergoing haemodialysis therapy. The values obtained have been compared with those measured in non-haemodialysed patients. At the end of a 10-h dialysis period, the total CSF amino acid concentration had fallen by only 23.3% compared with a 57.7% fall in plasma. Examination of the results for individual amino acid concentrations showed 3 different patterns of inter-relationship between plasma and CSF.     

Immunoglobulins in myasthenia gravis: effect of human lymph IgG 3 and F (ab')2 fragments on a cholinergic receptor preparation from Torpedo marmorata.

Lymph IgG from patients with myasthenia gravis was shown to inhibit the binding of radio-labelled Naja naja siamensis alpha-neurotoxin to membrane fragments from the electric organ of Torpedo marmorata. This effect could be related to IgG subclass 3 and to the F(ab')2 fragment. These findings confirm the involvement of antibodies of postsynaptic structures in myasthenia gravis.     

The effect of sucrose and other carbohydrates on human alkaline phosphatase isoenzyme activity.

The modification of alkaline phosphatase (ALP) activity by ten carbohydrates (d-isomers) was studied. These included three disaccharides : sucrose, lactose and maltose; four hexoses: glucose, galactose, mannose and fructose; and three pentoses: arabinose, ribose and xylose.In all cases the inhibition of placental isoenzyme activity was in proportion to the concentration of carbohydrate. Intestinal and hepatic ALP was activated at low carbohydrate concentrations and inhibited at high concentration. The exception was maltose which inhibited hepatic alkaline phosphatase markedly at all concentrations.Lineweaver-Burk plots generated by kinetic studies at pH 9.8, 10.15, 10.5 and 10.7 showed a mixed-type inhibition.With the use of sucrose in the assay medium it was possible to distinguish two types of term placental-like ALP isoenzymes of tumor origin, which differ in their sensitivity to inhibition by l-leucine. The Nagao type (d-leucine sensitive) was inhibited more by sucrose than was the non-d-leucine sensitive isoenzyme.The inhibitory effect of carbohydrates on placental ALP activity was independent of pH and substrate concentration, unlike the modifying effect on the activity of intestinal and hepatic enzyme by the carbohydrates studied. It is suggested that carbohydrate-rich media may accentuate conformational differences of the various isoenzymes with consequent effects on the availability of the active sites to the substrate.