人偏肺病毒与呼吸道合胞病毒感染后细胞因子改变的比较

人偏肺病毒与呼吸道合胞病毒是呼吸道感染的常见病原体,其感染后的发病机制尚不完全清楚,广泛接受的假说是引起机体固有免疫反应.大量研究显示两者产生不同的细胞因子效应,也提示人偏肺病毒感染的发病机制可能有别于呼吸道合胞病毒.本文就目前国内外对人偏肺病毒感染后与呼吸道合胞病毒细胞因子改变的比较研究进行综述.     

也门儿童的人麦塔肺病毒和呼吸道合胞病毒

发展中国家中能够增加呼吸道感染严重性的因素很少被关注。我们报道的因素与发生在也门儿童的严重的呼吸系统疾病有关（266位呼吸合胞病毒感染和66位人麦塔肺病毒感染）．年龄．室内空气污染和不完全接种疫苗是危险因素．这不同于发达国家．     

人类偏肺病毒研究进展

研究表明,婴幼儿急性呼吸道感染（acute respiratory infection,ARI）的主要病原是人类呼吸道合胞病毒（hRSV）、流感病毒（甲、乙型）、副流感病毒（主要是Ⅰ、Ⅲ型）、肠道病毒和腺病毒,还有一部分病原尚未明了。     

呼吸道合胞病毒核酸检测阳性患儿的临床特征分析

目的分析呼吸道合胞病毒核酸(RSV-RNA)检测阳性患儿的临床特征,为临床诊断和治疗儿童呼吸道合胞病毒感染(RSV)提供依据。方法对深圳市盐田区人民医院2016-09~2017-09住院患儿中有呼吸道感染症状者进行咽拭子采样,提取RNA后用逆转录聚合酶链反应(RT-PCR)法检测RSV基因,测定病毒载量,同时抽血检测血常规和C反应蛋白(CRP)。结果 1 641例呼吸道感染患儿中RSV-RNA阳性114例,阳性率为6.95%。6个月、6个月~1岁、1岁~2岁这三组阳性率分别为14.52%、10.18%、10.96%,显著高于3岁~5岁组和≥5岁组,差异有统计学意义(P0.05);7~9月份组阳性率为15.01%,高于其他月份组,差异有统计学意义(P0.05);男童组阳性率为6.22%,女童组阳性率为7.94%,差异无统计学意义(P0.05);RSV-RNA阳性患者白细胞计数偏高占29.82%,白细胞计数正常占65.79%,白细胞计数偏低占4.39%;病毒载量在各年龄组、各性别组差异无统计学意义(P0.05);病毒载量与白细胞计数、中性粒细胞比例、淋巴细胞比例、CRP无显著相关性(P0.05)。结论 RSV-RNA检测对小儿呼吸道感染诊断具有重要价值,血常规及CRP检测对于是否病毒感染所能提供信息有限。     

人呼吸道合胞病毒转录调节基因转染肺腺癌细胞系的研究

目的获得稳定表达人呼吸道合胞病毒(hRSV)M2-1基因的人肺腺癌细胞系.方法通过基因重组法构建hRSV M2-1基因真核表达载体,用脂质体将其转染人肺腺癌PAa和A549细胞,经G418筛选获得阳性表达细胞株,并用逆转录-聚合酶链反应(RT-PCR)和Western Blot进行验证.结果得到了约650bp的基因插入片段,DNA测序表明该基因高度保守.筛选出了稳定高量表达M2-1基因的PAa和A549细胞,并被证实有M2-1蛋白表达.结论获得了稳定表达hRSV M2-1蛋白的PAa和A549细胞株.     

成人呼吸道合胞病毒下呼吸道感染临床特征和预后分析

目的 分析成人呼吸道合胞病毒(RSV)下呼吸道感染患者的临床特征及预后.方法 以2016年8月至2019年11月入住中日友好医院呼吸科或重症监护病房(ICU)的RSV核酸阳性患者148例为研究对象,同时取甲型流感病毒(FluA)核酸阳性的下呼吸道感染患者266例为对照组,根据是否合并免疫功能低下,分为免疫功能正常组和免...     

成人呼吸道合胞病毒的电镜和免疫电镜研究

本研究主要探讨呼吸道合胞病毒（ＲＳＶ）成人感染的电镜诊断。应用常规电镜和金标记电镜技术观察９７例ＲＳＶ阳性患者鼻咽部脱落细胞。一些标本的脱落上皮细胞表面在常规电镜下查见ＲＳＶ形态特征的病毒颗粒，免疫电镜可见金颗粒特异性标记。提示，ＲＳＶ是成人呼吸道感染的病原之一。     

97例婴幼儿合胞病毒下呼吸道感染的临床及治疗

合胞病毒(RSV)下呼吸道感染是儿科常见病,由于其转归与哮喘关系密切,故越来越受到重视。本文收集了我院1994年1月至1998年3月间97例RSV下呼吸道感染病例,现报告如下。     

Absence of human metapneumovirus co-infection in cases of severe respiratory syncytial virus infection

It has been suggested that co-infection of human metapneumovirus (hMPV) in severe respiratory syncytial (RSV) virus bronchiolitis is very common. To evaluate the epidemiology of hMPV co-infection in children with severe lower respiratory tract infection caused by RSV virus. This was an observational cohort study in which hMPV and RSV viral load was measured by RT-PCR in tracheal specimens from the target population. hMPV could not be detected in any of the 30 mechanically ventilated children with RSV lower respiratory tract infection. Our study suggests that hMPV co-infection is not very common in severe RSV lower respiratory tract infection.     

Human metapneumovirus and respiratory syncytial virus infections in older children with cystic fibrosis

BACKGROUND: Human metapneumovirus (hMPV) has been isolated from children with acute respiratory infection worldwide. Its epidemiology remains to be defined in children with cystic fibrosis (CF). We describe the epidemiology and clinical impact of hMPV in CF children and compared it to respiratory syncytial virus (RSV). METHODS: CF children ages 7-18 years were studied prospectively during the 1998 -1999 RSV season. Nasopharyngeal specimens were collected during acute respiratory illnesses and tested for respiratory viruses. Blood specimens were drawn early, mid, and end of the RSV season, and tested for serological evidence of hMPV and RSV infections. Rates of lower respiratory tract illnesses (LRTI) and hospitalizations for pulmonary exacerbations were compared during the time intervals they developed serological evidence of infection to their non-infection intervals. RESULTS: Six of 44 CF children had a virus positive respiratory illness in 56 LTRI events and 18 hospitalizations. Serological evidence of hMPV and RSV infections occurred in 16 and 20 CF children, respectively; 8 had infections with both viruses. A greater proportion of CF children had >or=1 LRTI during their infection intervals compared to their non-infection intervals (13/25 vs. 5/25; P=0.03). A trend for higher rates of LRTI was observed in the infection intervals compared to non-infection intervals (9.5 +/- 11.0 vs. 4.2 +/- 9.9 per 1,000 child-days; P=0.06), and it was significantly greater with a more conservative estimate (one event per child per interval; 7.4 +/- 7.7 vs. 2.6 +/- 5.4 per 1,000 child-days; P 相似文献   

Acute lower respiratory tract infections by human metapneumovirus in children in Southwest China: A 2‐year study

Human metapneumovirus (hMPV) has been reported to cause both upper and lower respiratory tract diseases in susceptible populations, particularly in children and the elderly. In this study, we describe a hospital‐based epidemiological study of hMPV in patients presenting to a children's hospital and show the demographic and clinical characteristics associated with hMPV infection in China, retrospectively. Specimens were collected over a 2‐year period from children hospitalized with acute lower respiratory tract infections (ALRTI) and analyzed for the presence of hMPV using real‐time RT‐PCR assays. The presence of hMPV was detected in 227 (25.9%) of the 878 children studied and may circulate year‐round in the area, peaking during the winter–spring season. Younger children (aged less than 6 months) had the highest positive rate. Infections by hMPV showed similar epidemiology and clinical manifestations as for respiratory syncytial virus (RSV) and were found in high co‐infections with RSV. Subgroup A2 hMPV was the most predominant genotype identified during the study period. This study indicates that hMPV is one of the major respiratory pathogens found in children in southwest China and vaccine development should be under consideration. Pediatr. Pulmonol. 2010; 45:824–831. © 2010 Wiley‐Liss, Inc.     

Retrospective comparison of respiratory syncytial virus and metapneumovirus clinical presentation in hospitalized children

Respiratory syncytial virus (RSV) and Human metapneumovirus (hMPV), members of Pneumoviridae family are common causes of acute respiratory tract infections (ARTI) among children. Study material includes routine nasopharyngeal samples obtained during 8-year period for hMPV and one single season for RSV in children hospitalized for ARTI between 0 and 15 years at the Center Hospitalier Universitaire (CHU) Saint Pierre in Brussels. Positive samples for RSV or hMPV identified by viral culture, lateral flow chromatography test for RSV or direct fluorescent assay for hMPV were selected retrospectively. Characteristics of children hospitalized for RSV or hMPV infections were compared. Children hospitalized for RSV infection were significantly younger and requiring more respiratory support, longer hospital stay and transfers in Pediatric intensive Care Units than those hospitalized for hMPV infection. Pneumonia diagnostic and antibiotics therapies were more significantly associated with hMPV infections. In conclusion, despite their genetic similarities, RSV, and hMPV present epidemiological and clinical differences in pediatric infections. Our results should be confirmed prospectively.     

Impact of human metapneumovirus and respiratory syncytial virus co-infection in severe bronchiolitis

We have previously shown high rates of co-infection with Respiratory Syncytial Virus (RSV) and human Metapneumovirus (hMPV) in infants with severe bronchiolitis at our institution in 2000-2002, and that co-infection was associated with increased disease severity. In this study, we have attempted to identify differences in intubated infants with severe RSV infection with and without hMPV co-infection. Here we show that RSV+/hMPV+ were clinically symptomatic for longer than RSV+/hMPV- infants, but that no differences in airway total cell concentration, differential cell count or cytokine/chemokine concentrations were detectable.